PE20120856A1 - Sondas de oligonucleotidos y cebadores para la deteccion del virus de la hepatitis b - Google Patents

Sondas de oligonucleotidos y cebadores para la deteccion del virus de la hepatitis b

Info

Publication number
PE20120856A1
PE20120856A1 PE2011001490A PE2011001490A PE20120856A1 PE 20120856 A1 PE20120856 A1 PE 20120856A1 PE 2011001490 A PE2011001490 A PE 2011001490A PE 2011001490 A PE2011001490 A PE 2011001490A PE 20120856 A1 PE20120856 A1 PE 20120856A1
Authority
PE
Peru
Prior art keywords
seq
primers
hepatitis
virus
oligonucleotide probes
Prior art date
Application number
PE2011001490A
Other languages
English (en)
Inventor
Manjula Jagannath
Chandrasekhar Bhaskaran Nair
Pillarisetti Venkata Subbarao
Original Assignee
Bigtec Private Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bigtec Private Ltd filed Critical Bigtec Private Ltd
Publication of PE20120856A1 publication Critical patent/PE20120856A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SE REFIERE A UN METODO PARA DETECTAR EL VIRUS DE LA HEPATITIS B QUE COMPRENDE: A) FORMAR UNA MEZCLA DE REACCION QUE COMPRENDE i) REACTIVOS DE AMPLIFICACION DE ACIDO NUCLEICO TAL COMO CLORURO DE MAGNESIO, TAQ POLIMERASA Y TAMPON PARA AMPLIFICACION, ii) UNA MUESTRA DE PRUEBA TAL COMO SANGRE, SUERO O PLASMA, iii) SONDAS DE OLIGONUCLEOTIDOS QUE COMPRENDEN LA SEQ ID Nº 1 Y 2 CONJUGADAS CON MARCADORES DETECTABLES TALES COMO UN FLUOROFORO EN EL EXTREMO 5' QUE ES 6-CARBOXIFLUORESCEINA Y UN EXTINTOR EN EL EXTREMO 3' QUE ES TETRAMETILRODAMINA, Y iv) CEBADORES QUE COMPRENDEN LA SEQ ID Nº 3, 4, 5 Y 6 CORRESPONDIENDO LOS CEBADORES QUE TIENEN SEQ ID Nº 3 Y 4 A LA SONDA CON MARCAJE DOBLE DE SEQ ID Nº 1 Y LOS CEBADORES QUE TIENEN SEQ ID Nº 5 Y 6 A LA SONDA CON MARCAJE DOBLE DE SEQ ID Nº 2; Y B) SOMETER LA MEZCLA DE REACCION A PCR PARA OBTENER COPIAS DE LA SECUENCIA DIANA SEGUIDO DE LA MEDICION DEL AUMENTO DE LA SENAL DE FLUORESCENCIA PARA DETECTAR EL VIRUS DE LA HEPATITIS B
PE2011001490A 2009-02-13 2010-01-27 Sondas de oligonucleotidos y cebadores para la deteccion del virus de la hepatitis b PE20120856A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IN314CH2009 2009-02-13

Publications (1)

Publication Number Publication Date
PE20120856A1 true PE20120856A1 (es) 2012-07-23

Family

ID=42562138

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2011001490A PE20120856A1 (es) 2009-02-13 2010-01-27 Sondas de oligonucleotidos y cebadores para la deteccion del virus de la hepatitis b

Country Status (20)

Country Link
US (1) US20110306037A1 (es)
EP (1) EP2396428A4 (es)
JP (1) JP2012517804A (es)
KR (1) KR20110117224A (es)
CN (1) CN102317474A (es)
AP (1) AP2011005866A0 (es)
AU (1) AU2010213426A1 (es)
CA (1) CA2751745A1 (es)
CL (1) CL2011001953A1 (es)
CO (1) CO6420362A2 (es)
EA (1) EA201171052A1 (es)
IL (1) IL214514A0 (es)
MA (1) MA33040B1 (es)
MX (1) MX2011008587A (es)
NZ (1) NZ594574A (es)
PE (1) PE20120856A1 (es)
SG (1) SG173569A1 (es)
TN (1) TN2011000401A1 (es)
UA (1) UA99793C2 (es)
WO (1) WO2010092595A2 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101678553B1 (ko) * 2012-04-30 2016-11-22 (주)바이오니아 엡스타인-바 바이러스 검출용 키트 및 이를 이용한 엡스타인-바 바이러스의 검출방법
TWI586809B (zh) * 2014-11-10 2017-06-11 Taichung Veterans General Hospital In vitro detection and / or quantification of hepatitis B virus and its use of the primer, probe
CN105861649A (zh) * 2016-04-06 2016-08-17 苏州华益美生物科技有限公司 一种新型核酸扩增反应中的探针及应用
CN110088302A (zh) * 2016-11-21 2019-08-02 简·探针公司 用于检测或定量乙型肝炎病毒的组合物和方法
CA3068477A1 (en) * 2017-09-27 2019-04-04 Abbott Molecular Inc. Assay for detecting hepatitis b virus (hbv)
CN111808985A (zh) * 2019-04-11 2020-10-23 北京大学 超敏检测NAs治疗下乙型肝炎病毒DNA的寡核苷酸组合物、试剂盒、方法及用途
CN111154920A (zh) * 2020-03-09 2020-05-15 北京康美天鸿生物科技有限公司 一种用于检测乙型肝炎病毒的试剂盒及其专用引物探针组

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11262399A (ja) * 1998-03-17 1999-09-28 Srl Inc Ｂ型肝炎ウイルス検出用プライマー及びそれを用いたｂ型肝炎ウイルスの検出方法
JP4399048B2 (ja) * 1999-01-13 2010-01-13 財団法人 東京都医学研究機構 リアルタイム検出ｐｃｒ法によるｈｂｖ遺伝子の測定方法並びにそれに用いられるプライマー及びプローブ
WO2003031934A2 (en) * 2001-10-09 2003-04-17 Chiron Corporation Identification of oligonucleotides for the capture, detection and quantitation of hepatitis b viral dna
US7015317B2 (en) * 2002-05-02 2006-03-21 Abbott Laboratories Polynucleotides for the detection and quantification of hepatitis B virus nucleic acids
US20040058314A1 (en) * 2002-05-29 2004-03-25 Ming Liang He Assay for the detection and quantification of HBV cccDNA by real-time PCR
CN1392268A (zh) * 2002-06-11 2003-01-22 赵伟 一种检测多种肝炎的检测型基因芯片
KR100647277B1 (ko) * 2003-08-14 2006-11-17 삼성전자주식회사 Ｂ형 간염 검사용 ｐｃｒ 프라이머 세트 및 이를 포함하는 ｂ형 간염 검사 키트
JP4708045B2 (ja) * 2005-02-10 2011-06-22 株式会社エスアールエル Ｂ型肝炎ウイルスｃｃｃＤＮＡの測定方法並びにそのためのプライマー及びプローブ
GB0519169D0 (en) * 2005-09-21 2005-10-26 Leuven K U Res & Dev Novel anti-viral strategy
CN100469895C (zh) * 2006-06-12 2009-03-18 山东省医药生物技术研究中心 用于乙肝病毒的荧光定量pcr检测试剂盒
JP2009213465A (ja) * 2007-10-30 2009-09-24 Toshiba Corp Ｂ型肝炎ウイルス薬剤耐性株検出用核酸プライマーセット、アッセイキットおよびｂ型肝炎ウィルスの薬剤耐性株の検出方法
WO2009122422A1 (en) * 2008-03-31 2009-10-08 Bigtec Private Limited Probes and primers for detection of hepatitis b virus and a method thereof

Also Published As

Publication number Publication date
WO2010092595A2 (en) 2010-08-19
CN102317474A (zh) 2012-01-11
CA2751745A1 (en) 2010-08-19
EP2396428A2 (en) 2011-12-21
IL214514A0 (en) 2011-09-27
EA201171052A1 (ru) 2012-02-28
SG173569A1 (en) 2011-09-29
CL2011001953A1 (es) 2012-04-20
AP2011005866A0 (en) 2011-10-31
AU2010213426A1 (en) 2011-09-01
MX2011008587A (es) 2011-09-06
CO6420362A2 (es) 2012-04-16
MA33040B1 (fr) 2012-02-01
EP2396428A4 (en) 2012-07-25
TN2011000401A1 (en) 2013-03-27
NZ594574A (en) 2012-06-29
US20110306037A1 (en) 2011-12-15
UA99793C2 (ru) 2012-09-25
JP2012517804A (ja) 2012-08-09
KR20110117224A (ko) 2011-10-26
WO2010092595A3 (en) 2010-10-14

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