BR112013027876A2 - "métodos para detectar uma sequência de ácido nucleico alvo e kits para detectar uma sequência de ácido nucleico alvo" - Google Patents

"métodos para detectar uma sequência de ácido nucleico alvo e kits para detectar uma sequência de ácido nucleico alvo"

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Publication number
BR112013027876A2
BR112013027876A2 BR112013027876A BR112013027876A BR112013027876A2 BR 112013027876 A2 BR112013027876 A2 BR 112013027876A2 BR 112013027876 A BR112013027876 A BR 112013027876A BR 112013027876 A BR112013027876 A BR 112013027876A BR 112013027876 A2 BR112013027876 A2 BR 112013027876A2
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BR
Brazil
Prior art keywords
nucleic acid
target nucleic
acid sequence
cleavage
present
Prior art date
Application number
BR112013027876A
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English (en)
Other versions
BR112013027876B1 (pt
Inventor
Jong Yoon Chun
Young Jo Lee
Original Assignee
Seegene Inc
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Publication date
Application filed by Seegene Inc filed Critical Seegene Inc
Publication of BR112013027876A2 publication Critical patent/BR112013027876A2/pt
Publication of BR112013027876B1 publication Critical patent/BR112013027876B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

resumo patente de invenção: "detecção de sequências de ácido nucleico alvo por clivagem e hibridização de po". a presente invenção refere-se à detecção de uma sequência de ácido nucleico alvo por um ensaio de poch (clivagem e hibridização de po) em um substrato sólido. a presente invenção detecta a sequência de ácido nucleico alvo pelo uso de em que o po (oligonucleotídeo de sondagem) hibridizado com a sequência de ácido nucleico alvo é clivado e a clivagem do po é detectada por hibridização com o co (oligonucleotídeo de captura). na presente invenção, um po não clivado é hibridizado com o co imobilizado sobre o substrato sólido. os projetos do po e o co são convenientes e a otimização das condições de reação é rotineiramente fácil na presente invenção. onde a detecção do sinal no substrato sólido é continuamente realizada junto com a repetição da clivagem dos pos na presente invenção, o número dos pos clivados é aumentado no número de repetição da reação de clivagem e o sinal é mudado em paralelo com o número dos pos clivados. depois, a sequência de ácido nucleico alvo pode ser detectada em uma maneira em tempo real. ao contrário, a mudança do sinal não é observada na ausência da sequência de ácido nucleico alvo.
BR112013027876-5A 2011-05-04 2012-05-03 métodos para detectar uma sequência de ácido nucleico alvo e kits para detectar uma sequência de ácido nucleico alvo BR112013027876B1 (pt)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
KR20110042332 2011-05-04
KR10-2011-0042332 2011-05-04
KR20110068888 2011-07-12
KR10-2011­0068888 2011-07-12
PCT/KR2011/009317 WO2012150749A1 (en) 2011-05-04 2011-12-02 Detection of target nucleic acid sequences by po cleavage and hybridization
KRPCT/KR2011/009317 2011-12-02
PCT/KR2012/003497 WO2012150835A2 (en) 2011-05-04 2012-05-03 Detection of target nucleic acid sequences by po cleavage and hybridization

Publications (2)

Publication Number Publication Date
BR112013027876A2 true BR112013027876A2 (pt) 2016-11-29
BR112013027876B1 BR112013027876B1 (pt) 2021-06-08

Family

ID=47107925

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013027876-5A BR112013027876B1 (pt) 2011-05-04 2012-05-03 métodos para detectar uma sequência de ácido nucleico alvo e kits para detectar uma sequência de ácido nucleico alvo

Country Status (13)

Country Link
EP (1) EP2705163B1 (pt)
JP (2) JP5993939B2 (pt)
KR (1) KR101622780B1 (pt)
CN (1) CN103620056B (pt)
AU (1) AU2012251222B2 (pt)
BR (1) BR112013027876B1 (pt)
CA (1) CA2834030C (pt)
ES (1) ES2657906T3 (pt)
IL (1) IL229132B (pt)
MX (1) MX342067B (pt)
NO (1) NO2705163T3 (pt)
RU (1) RU2016104218A (pt)
WO (2) WO2012150749A1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8703653B2 (en) 2011-02-18 2014-04-22 NVS Technologies, Inc. Quantitative, highly multiplexed detection of nucleic acids
KR101760267B1 (ko) * 2012-12-27 2017-07-24 주식회사 씨젠 Pto 절단 및 연장-의존적 비-혼성화 어세이에 의한 타겟 핵산서열의 검출
EP3022319B1 (en) * 2013-07-15 2023-05-17 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
EP3027772B1 (en) 2013-07-31 2019-03-06 Seegene, Inc. Method for determining snp genotype
CN105705657B (zh) * 2013-10-18 2020-07-28 Seegene株式会社 基于利用杂交-捕捉和模板化寡核苷酸的探测和标记寡核苷酸切割及延伸分析的在固相中的靶核酸序列检测
KR102465287B1 (ko) * 2014-08-11 2022-11-09 루미넥스 코포레이션 핵산 검정에서 개선된 용융 판별 및 멀티플렉싱을 위한 프로브
US11066704B2 (en) 2016-06-10 2021-07-20 Seegene, Inc. Methods for preparing tagging oligonucleotides
KR102264865B1 (ko) 2016-06-30 2021-06-14 주식회사 씨젠 반응 분석용 형광 검출 장치
WO2022136875A1 (en) * 2020-12-23 2022-06-30 Biofidelity Ltd Improved polynucleotide sequence detection method

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4351760A (en) 1979-09-07 1982-09-28 Syva Company Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DK51092D0 (da) 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
US5665539A (en) 1991-07-12 1997-09-09 The Regents Of The University Of California Immuno-polymerase chain reaction system for antigen detection
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US5541311A (en) 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
ES2192672T3 (es) 1996-11-18 2003-10-16 Takeshi Imanishi Nuevos analogos de nucleotidos.
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
CN1273476C (zh) 1997-09-12 2006-09-06 埃克西康有限公司 寡核苷酸类似物
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
DK1923471T3 (da) * 1999-04-20 2013-04-02 Illumina Inc Detektion af nukleinsyrereaktioner på bead-arrays
US7381532B2 (en) * 1999-10-29 2008-06-03 Stratagene California Compositions and methods for the detection of a nucleic acid using a cleavage reaction
GB0205455D0 (en) * 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US8206904B2 (en) * 2002-12-18 2012-06-26 Third Wave Technologies, Inc. Detection of nucleic acids
US20050191682A1 (en) * 2004-02-17 2005-09-01 Affymetrix, Inc. Methods for fragmenting DNA
WO2005086647A2 (en) 2004-02-23 2005-09-22 University Of Maryland, Baltimore Immuno-pcr method for the detection of a biomolecule in a test sample
PL1856257T3 (pl) 2005-03-05 2012-03-30 Seegene Inc Procesy wykorzystujące oligonukleotyd o podwójnej specyficzności oraz oligonukleotyd o podwójnej specyficzności
EP1777298A1 (en) * 2005-10-13 2007-04-25 Roche Diagnostics GmbH Multiplexed solid-phase nucleic acid amplification assay
CN101541975B (zh) * 2006-06-01 2013-04-03 第三次浪潮技术公司 核酸的检测
EP2546364A1 (en) 2006-07-17 2013-01-16 Brandeis University Specialized oligonucleotides and their use in nucleic acid amplification and detection
EP2118310B1 (en) * 2006-12-29 2013-03-06 Applied Biosystems, LLC Systems and methods for detecting nucleic acids
WO2008083259A1 (en) * 2006-12-29 2008-07-10 Applera Corporation Systems and methods for detecting nucleic acids
EP2060637A1 (en) * 2007-11-14 2009-05-20 Koninklijke Philips Electronics N.V. Means and methods for detection of nucleic acids
KR20090067334A (ko) * 2007-12-21 2009-06-25 주식회사 씨젠 핵산 중합효소의 뉴클레아제 활성을 이용한 타깃핵산분자의 검출방법
EP2256215A1 (en) * 2009-04-30 2010-12-01 Steffen Mergemeier Assay system using a nuclease activity of a nucleic acid polymerase
WO2011027966A2 (en) 2009-09-03 2011-03-10 Seegene, Inc. Td probe and its uses
WO2011037306A1 (en) * 2009-09-24 2011-03-31 Seegene, Inc. Detection of target nucleic acid sequences by cyclic exonucleolytic reactions

Also Published As

Publication number Publication date
MX342067B (es) 2016-09-09
WO2012150835A3 (en) 2013-03-21
KR101622780B1 (ko) 2016-05-19
IL229132A0 (en) 2013-12-31
ES2657906T3 (es) 2018-03-07
JP6529934B2 (ja) 2019-06-12
RU2016104218A (ru) 2018-11-22
IL229132B (en) 2018-03-29
EP2705163A2 (en) 2014-03-12
EP2705163A4 (en) 2014-12-31
BR112013027876B1 (pt) 2021-06-08
WO2012150835A2 (en) 2012-11-08
CN103620056A (zh) 2014-03-05
EP2705163B1 (en) 2017-11-01
CA2834030A1 (en) 2012-11-08
RU2013152434A (ru) 2015-06-10
AU2012251222A1 (en) 2013-11-14
JP2014515616A (ja) 2014-07-03
CN103620056B (zh) 2016-06-01
JP5993939B2 (ja) 2016-09-21
WO2012150749A1 (en) 2012-11-08
RU2016104218A3 (pt) 2018-11-22
NO2705163T3 (pt) 2018-03-31
MX2013012901A (es) 2014-07-28
JP2016195603A (ja) 2016-11-24
CA2834030C (en) 2017-03-28
KR20130137703A (ko) 2013-12-17
AU2012251222B2 (en) 2016-02-11

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06T Formal requirements before examination [chapter 6.20 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 03/05/2012, OBSERVADAS AS CONDICOES LEGAIS.