BR112012026221A2 - métodos e kit para detectar sequência de ácido nucléico-alvo de um dna ou uma mistura de ácidos nucleicos através de um ensaio de ptoce - Google Patents

métodos e kit para detectar sequência de ácido nucléico-alvo de um dna ou uma mistura de ácidos nucleicos através de um ensaio de ptoce

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Publication number
BR112012026221A2
BR112012026221A2 BR112012026221A BR112012026221A BR112012026221A2 BR 112012026221 A2 BR112012026221 A2 BR 112012026221A2 BR 112012026221 A BR112012026221 A BR 112012026221A BR 112012026221 A BR112012026221 A BR 112012026221A BR 112012026221 A2 BR112012026221 A2 BR 112012026221A2
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Brazil
Prior art keywords
nucleic acid
acid sequence
target nucleic
detecting
extended duplex
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BR112012026221A
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English (en)
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BR112012026221B1 (pt
Inventor
Jong Yoo Chun
Young Jo Lee
Original Assignee
Seegene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Priority claimed from KR1020110002840A external-priority patent/KR20120081482A/ko
Priority claimed from KR1020110023465A external-priority patent/KR20120105811A/ko
Application filed by Seegene Inc filed Critical Seegene Inc
Priority to BR122019022355-6A priority Critical patent/BR122019022355B1/pt
Priority to BR122019022348-3A priority patent/BR122019022348B1/pt
Publication of BR112012026221A2 publication Critical patent/BR112012026221A2/pt
Publication of BR112012026221B1 publication Critical patent/BR112012026221B1/pt

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    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/521—Single-layer analytical elements
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm

Abstract

detecção de sequências de ácidos nucléicos alvos através da clivagem de pto e do ensaio de extensão. a presente invenção refere-se à detecção de uma sequência de ácido nucléico alvo através de um ensaio de ptoce (clivagem e extensão com pto). a presente invenção detecta uma sequência de ácido nucléico alvo em que o pto (oligonucleotídeo de sondagem e marcação) hibridizado com a sequência de ácido nucléico alvo é clivado para liberar um fragmento e o fragmento é hibridizado com o cto (oligonucleotídeo de captura e molde) para formar um duplex estendido, seguido pela detecção da presença do duplex estendido. o duplex estendido fornece sinais (geração, aumento, extinção ou decréscimo de sinais) partindo das marcações que indicam a presença do duplex estendido e possui valor de t~ m~ ajustável, que são bem adotáveis para a detecção da presença da sequência de ácido nucléico alvo.
BR112012026221-1A 2011-01-11 2012-01-11 método e kit para detectar uma sequência de ácido nucleico alvo de um dna ou uma mistura de ácidos nucleicos através de um ensaio de ptoce BR112012026221B1 (pt)

Priority Applications (2)

Application Number Priority Date Filing Date Title
BR122019022355-6A BR122019022355B1 (pt) 2011-01-11 2012-01-11 método e kit para detectar uma sequência de ácido nucleicoalvo de um dna ou uma mistura de ácidos nucleicos através de um ensaio de ptoce
BR122019022348-3A BR122019022348B1 (pt) 2011-01-11 2012-01-11 método e kit para detectar uma sequência de ácido nucleico-alvo de um dna ou uma mistura de ácidos nucleicos através de um ensaio de ptoce

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
KR10-2011-0002840 2011-01-11
KR1020110002840A KR20120081482A (ko) 2011-01-11 2011-01-11 태그 프로브 절단 및 연장에 의한 타겟 핵산서열의 검출
KR10-2011-0023465 2011-03-16
KR1020110023465A KR20120105811A (ko) 2011-03-16 2011-03-16 Ｐｔｏ 절단 및 연장-의존적 시그널 발생에 의한 타겟 핵산서열의 검출
PCT/KR2011/004452 WO2012096430A1 (en) 2011-01-11 2011-06-17 Detection of target nucleic acid sequences by pto cleavage and extension assay
KRPCT/KR2011/004452 2011-06-17
PCT/KR2012/000287 WO2012096523A2 (en) 2011-01-11 2012-01-11 Detection of target nucleic acid sequences by pto cleavage and extension assay

Publications (2)

Publication Number Publication Date
BR112012026221A2 true BR112012026221A2 (pt) 2017-01-10
BR112012026221B1 BR112012026221B1 (pt) 2021-03-02

Family

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Family Applications (3)

Application Number Title Priority Date Filing Date
BR122019022355-6A BR122019022355B1 (pt) 2011-01-11 2012-01-11 método e kit para detectar uma sequência de ácido nucleicoalvo de um dna ou uma mistura de ácidos nucleicos através de um ensaio de ptoce
BR112012026221-1A BR112012026221B1 (pt) 2011-01-11 2012-01-11 método e kit para detectar uma sequência de ácido nucleico alvo de um dna ou uma mistura de ácidos nucleicos através de um ensaio de ptoce
BR122019022348-3A BR122019022348B1 (pt) 2011-01-11 2012-01-11 método e kit para detectar uma sequência de ácido nucleico-alvo de um dna ou uma mistura de ácidos nucleicos através de um ensaio de ptoce

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BR122019022355-6A BR122019022355B1 (pt) 2011-01-11 2012-01-11 método e kit para detectar uma sequência de ácido nucleicoalvo de um dna ou uma mistura de ácidos nucleicos através de um ensaio de ptoce

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BR122019022348-3A BR122019022348B1 (pt) 2011-01-11 2012-01-11 método e kit para detectar uma sequência de ácido nucleico-alvo de um dna ou uma mistura de ácidos nucleicos através de um ensaio de ptoce

Country Status (22)

Country Link
US (6) US8809239B2 (pt)
EP (6) EP3168311B1 (pt)
JP (3) JP5756854B2 (pt)
KR (1) KR101462192B1 (pt)
CN (2) CN103866037B (pt)
AU (1) AU2012205956B2 (pt)
BR (3) BR122019022355B1 (pt)
CA (3) CA2979858C (pt)
DK (1) DK2572004T3 (pt)
ES (3) ES2629759T3 (pt)
IL (2) IL223608A (pt)
IN (1) IN2013CN00284A (pt)
MX (3) MX340258B (pt)
MY (1) MY174052A (pt)
NO (1) NO343803B1 (pt)
PL (2) PL2572004T3 (pt)
PT (2) PT2572004E (pt)
RU (1) RU2566562C2 (pt)
SG (1) SG190758A1 (pt)
UA (1) UA115082C2 (pt)
WO (2) WO2012096430A1 (pt)
ZA (1) ZA201209536B (pt)

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