MX340258B - Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto. - Google Patents

Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.

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Publication number
MX340258B
MX340258B MX2013001510A MX2013001510A MX340258B MX 340258 B MX340258 B MX 340258B MX 2013001510 A MX2013001510 A MX 2013001510A MX 2013001510 A MX2013001510 A MX 2013001510A MX 340258 B MX340258 B MX 340258B
Authority
MX
Mexico
Prior art keywords
nucleic acid
target nucleic
detection
acid sequence
extended duplex
Prior art date
Application number
MX2013001510A
Other languages
English (en)
Other versions
MX2013001510A (es
Inventor
Jong Yoon Chun
Young Jo Lee
Original Assignee
Seegene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020110002840A external-priority patent/KR20120081482A/ko
Priority claimed from KR1020110023465A external-priority patent/KR20120105811A/ko
Application filed by Seegene Inc filed Critical Seegene Inc
Publication of MX2013001510A publication Critical patent/MX2013001510A/es
Publication of MX340258B publication Critical patent/MX340258B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/521—Single-layer analytical elements
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a la detección de una secuencia de ácido nucleico objetivo mediante en ensayo PTOCE (Escisión y Extensión del PTO). La presente invención detecta una secuencia de ácido nucleico objetivo en la cual el PTO (Oligonucleótido de Sondeo y Marcaje) hibridado con la secuencia de ácido nucleico objetivo se escinde para liberar un fragmento y el fragmento se hibrida con el CTO (Oligonucleótido de Captura y Modelado) para formar un duplo extendido, seguido por la detección de la presencia del duplo extendido. El duplo extendido provee señales (generación, incremento, extinción o disminución de señales) de etiquetas que indican la presencia del duplo extendido y tiene un valor Tm ajustable, las cuales son bien adoptables para la detección de la presencia de la secuencia de ácido nucleico objetivo.
MX2013001510A 2011-01-11 2011-06-17 Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto. MX340258B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020110002840A KR20120081482A (ko) 2011-01-11 2011-01-11 태그 프로브 절단 및 연장에 의한 타겟 핵산서열의 검출
KR1020110023465A KR20120105811A (ko) 2011-03-16 2011-03-16 Ｐｔｏ 절단 및 연장-의존적 시그널 발생에 의한 타겟 핵산서열의 검출
PCT/KR2011/004452 WO2012096430A1 (en) 2011-01-11 2011-06-17 Detection of target nucleic acid sequences by pto cleavage and extension assay

Publications (2)

Publication Number Publication Date
MX2013001510A MX2013001510A (es) 2013-02-27
MX340258B true MX340258B (es) 2016-07-01

Family

ID=46507300

Family Applications (3)

Application Number Title Priority Date Filing Date
MX2016008168A MX352460B (es) 2011-01-11 2011-06-17 Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
MX2017015093A MX2017015093A (es) 2011-01-11 2011-06-17 Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
MX2013001510A MX340258B (es) 2011-01-11 2011-06-17 Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.

Family Applications Before (2)

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MX2016008168A MX352460B (es) 2011-01-11 2011-06-17 Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
MX2017015093A MX2017015093A (es) 2011-01-11 2011-06-17 Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.

Country Status (22)

Country Link
US (6) US8809239B2 (es)
EP (6) EP2572004B1 (es)
JP (3) JP5756854B2 (es)
KR (1) KR101462192B1 (es)
CN (2) CN102959092B (es)
AU (1) AU2012205956B2 (es)
BR (3) BR122019022355B1 (es)
CA (3) CA2979858C (es)
DK (1) DK2572004T3 (es)
ES (3) ES2628327T3 (es)
IL (2) IL223608A (es)
IN (1) IN2013CN00284A (es)
MX (3) MX352460B (es)
MY (1) MY174052A (es)
NO (1) NO343803B1 (es)
PL (2) PL2572004T3 (es)
PT (2) PT2708608T (es)
RU (1) RU2566562C2 (es)
SG (1) SG190758A1 (es)
UA (1) UA115082C2 (es)
WO (2) WO2012096430A1 (es)
ZA (1) ZA201209536B (es)

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101903535A (zh) 2007-03-28 2010-12-01 信号诊断公司 高解析度分析核酸以检测序列变异的系统和方法
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
MX352460B (es) 2011-01-11 2017-11-24 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
SG192720A1 (en) * 2011-02-18 2013-09-30 Nvs Technologies Inc Quantitative, highly multiplexed detection of nucleic acids
JP5852222B2 (ja) 2011-03-29 2016-02-03 シージーン アイエヌシー Ｐｔｏ切断及び延長−依存的切断によるターゲット核酸配列の検出
US9850524B2 (en) 2011-05-04 2017-12-26 Seegene, Inc. Detection of target nucleic acid sequences by PO cleavage and hybridization
EP2710172B1 (en) * 2011-05-20 2017-03-29 Fluidigm Corporation Nucleic acid encoding reactions
DE102011055247A1 (de) 2011-11-10 2013-05-16 Albert-Ludwigs-Universität Freiburg Multianalyt-Reportersystem
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
KR101794981B1 (ko) * 2012-03-05 2017-11-09 주식회사 씨젠 Ｐｔｏ 절단 및 연장 어세이에 의한 타겟 핵산서열에서의 뉴클레오타이드 변이 검출
US9683259B2 (en) * 2012-04-19 2017-06-20 Seegene, Inc. Detection of target nucleic acid sequence by PTO cleavage and extension-dependent signaling oligonucleotide cleavage
JP2015524670A (ja) * 2012-08-16 2015-08-27 エヌブイエス テクノロジーズ，インコーポレイティド アッセイ方法及び系
JP6178430B2 (ja) 2012-12-27 2017-08-09 シージーン アイエヌシー Ｐｔｏ切断及び延長依存的非ハイブリダイゼーションアッセイによるターゲット核酸配列の検出
ES2662825T3 (es) * 2013-02-25 2018-04-09 Seegene, Inc. Detección de variación de nucleótido en una secuencia de ácidos nucleicos diana
JP2016508733A (ja) * 2013-03-13 2016-03-24 シージーン アイエヌシー メルティングピーク分析を利用したターゲット核酸配列の定量
AU2013202788B2 (en) 2013-03-14 2015-10-01 Gen-Probe Incorporated Indexing signal detection module
AU2013202805B2 (en) 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
EP3006938B1 (en) * 2013-05-30 2017-11-22 K-Mac Real time quantitative and qualitative analysis method for biosubstance
EP3022319B1 (en) * 2013-07-15 2023-05-17 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
WO2015016612A1 (en) * 2013-07-31 2015-02-05 Seegene, Inc. Method for determining snp genotype
ES2925313T3 (es) 2013-10-09 2022-10-14 Fluoresentric Inc Método para la detección de secuencias de ácido nucleico diana
WO2015057008A2 (en) 2013-10-18 2015-04-23 Seegene, Inc. Detection of target nucleic acid sequence on solid phase by pto cleavage and extension using hcto assay
EP2986719B1 (en) 2013-12-06 2020-07-29 Bio-Rad Laboratories, Inc. Fusion polymerases
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
KR102295290B1 (ko) * 2014-07-10 2021-08-30 플루어레센트릭 인코포레이티드 Dna 증폭 기술
DE102014110535A1 (de) 2014-07-25 2016-01-28 Certoplast Vorwerk & Sohn Gmbh Verfahren zur Herstellung eines Klebebandes
WO2016025452A1 (en) 2014-08-11 2016-02-18 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acids assays
KR101750464B1 (ko) 2014-11-28 2017-06-28 케이맥바이오센터주식회사 바이오물질의 실시간 정량 및 정성 분석을 위한 프로브 시스템, 상기 프로브 시스템을 구비한 반응용기 및 이를 이용한 분석 방법
FR3029290B1 (fr) 2014-12-01 2020-03-06 Biomerieux Procede d'estimation de l'affinite sonde-cible d'une puce a adn et procede de fabrication d'une puce a adn
CN107208139B (zh) 2014-12-09 2020-11-10 Seegene株式会社 与靶核酸序列有关的信号的分辨
WO2016093619A1 (en) * 2014-12-09 2016-06-16 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures and reference values
WO2017046192A1 (de) * 2015-09-18 2017-03-23 Biotype Diagnostic Gmbh Bestätigungstest für primäre nukleinsäure-amplifikate in einem kontinuierlichen reaktionsansatz und unmittelbare auswertung mittels elektrophoretischer verfahren
DE102015115836A1 (de) * 2015-09-18 2017-03-23 Biotype Diagnostic Gmbh Bestätigungstest für primäre Nukleinsäure-Amplifikate in einem kontinuierlichen Reaktionsansatz und unmittelbare Auswertung mittels immunchromatographischer Verfahren
EP3378000B1 (en) 2015-11-20 2021-09-01 Seegene, Inc. Method for calibrating a data set of a target analyte
KR102110985B1 (ko) 2015-12-15 2020-05-14 주식회사 씨젠 타겟 핵산 서열에 대한 시그널 추출
CA3006994A1 (en) 2015-12-16 2017-06-22 Fluidigm Corporation High-level multiplex amplification
KR102300738B1 (ko) 2016-02-05 2021-09-10 주식회사 씨젠 타겟 분석물질에 대한 데이터 세트의 노이즈 수준 감축 방법
EP3469100B1 (en) 2016-06-10 2021-01-27 Seegene, Inc. Methods for preparing tagging oligonucleotides
KR102301305B1 (ko) 2016-06-30 2021-09-13 주식회사 씨젠 핵산 증폭용 튜브 및 핵산 증폭용 반응 용기
JP7063886B2 (ja) 2016-09-15 2022-05-09 エフ．ホフマン－ラ ロシュ アーゲー マルチプレックスリアルタイムｐｃｒを実施する方法
WO2018066950A1 (en) 2016-10-06 2018-04-12 Seegene, Inc. . Methods for preparing oligonucleotides for detecting target nucleic acid molecules in samples
CA3049833A1 (en) 2016-12-23 2018-06-28 Albert-Ludwigs-Universitat Freiburg Two-piece mediator probe
JP6938641B2 (ja) 2016-12-29 2021-09-22 シージーン アイエヌシー プライマーダイマー形成を減少させ、増幅効率を増加させる方法
RU2665631C2 (ru) * 2017-01-25 2018-09-03 Общество с ограниченной ответственностью "Секвойя Дженетикс" Способ специфической идентификации последовательностей днк
JP6846636B2 (ja) * 2017-02-28 2021-03-24 パナソニックＩｐマネジメント株式会社 核酸検出用オリゴヌクレオチド
KR102408564B1 (ko) 2017-03-28 2022-06-14 주식회사 씨젠 타겟 핵산 서열의 존재를 결정하기 위한 분석 시그널
CN108823287B (zh) * 2017-04-28 2019-04-12 厦门大学 一种检测靶核酸序列的方法
DE102017210140A1 (de) 2017-06-16 2018-12-20 Fidlock Gmbh Verschlussvorrichtung zum lösbaren Verbinden zweier Teile
CN107236815A (zh) * 2017-07-18 2017-10-10 江西贤聚景欣医药生物科技有限公司 用于靶核酸序列检测的多重淬灭荧光探针及方法
WO2019045532A2 (en) 2017-08-31 2019-03-07 Seegene, Inc. EVALUATION OF COMPONENT PERFORMANCE USING A PAIR OF DIMER FORMER PRIMERS
AU2018339207A1 (en) 2017-09-28 2020-04-16 Seegene, Inc. . Method and device for analyzing target analyte in sample
US11401546B2 (en) * 2017-09-29 2022-08-02 Seegene, Inc. Detection of target nucleic acid sequences by PTO cleavage and extension-dependent extension assay
KR102369388B1 (ko) 2018-01-05 2022-03-02 주식회사 씨젠 시료에서 M. tuberculosis, M. bovis 및 M. bovis BCG의 존재 또는 부존재를 결정하는 방법
CA3088250A1 (en) 2018-01-22 2019-07-25 Luminex Corporation Methods and compositions for discrete melt analysis
EP3781708B1 (en) * 2018-04-20 2025-01-15 Seegene, Inc. Method for detecting a plurality of target nucleic acid sequences in sample
EP3895170B1 (en) 2018-12-14 2025-10-29 Seegene, Inc. Method for detecting a target analyte in a sample using an s-shaped function for a slope data set
WO2020127620A1 (en) 2018-12-20 2020-06-25 F. Hoffmann-La Roche Ag Detection of target nucleic acid by solid-phase molography
KR20230007464A (ko) 2020-06-05 2023-01-12 주식회사 씨젠 호흡기 병원체 검출을 위한 검체수송 키트 및 이를 이용한 호흡기 병원체 검출방법
EP4205124A4 (en) 2020-08-31 2024-08-28 Seegene, Inc. COMPUTER IMPLEMENTED METHOD FOR PROVIDING NUCLEIC ACID SEQUENCE DATASETS FOR OLIGONUCLEOTIDE DESIGN
US12497651B2 (en) * 2020-11-23 2025-12-16 Pleno, Inc. Encoded dual-probe endonuclease assays
EP4388132A1 (en) * 2021-08-19 2024-06-26 Luminex Corporation Digital amplification assay analysis method
KR20240058112A (ko) 2021-09-17 2024-05-03 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출
KR20240056605A (ko) 2021-09-30 2024-04-30 주식회사 씨젠 분자진단 시스템의 샘플 처리 및 분석 방법
EP4421814A4 (en) 2021-10-21 2025-11-12 Seegene Inc APPROVAL AND METHOD FOR READING A POSITIVE/NEGATIVE RESULT WITH RESPECT TO A TARGET TEST SUBSTANCE IN A SAMPLE
EP4424242A4 (en) 2021-10-29 2025-10-15 Seegene Inc SAMPLE COLLECTION SWAB TOOL AND METHOD FOR DETECTING A RESPIRATORY PATHOGEN
WO2023108014A1 (en) * 2021-12-07 2023-06-15 Luminex Corporation Methods and compositions for nucleic acid analysis
CN114032337B (zh) * 2021-12-14 2024-08-02 广州市金圻睿生物科技有限责任公司 一种呼吸道病原体检测试剂盒及其制备方法与应用
CN114134219A (zh) * 2021-12-14 2022-03-04 广州市金圻睿生物科技有限责任公司 一种多重核酸检测系统及其制备方法与应用
US20250145989A1 (en) 2022-01-26 2025-05-08 Seegene, Inc. Method for detecting target nucleic acids in sample
CA3247555A1 (en) * 2022-04-08 2023-10-12 Seegene, Inc. METHOD FOR DETECTING THREE TARGET NUCLEIC ACIDS IN A SAMPLE
EP4265734A1 (de) 2022-04-22 2023-10-25 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes
US20250340928A1 (en) 2022-04-22 2025-11-06 Albert-Ludwigs-Universität Freiburg Nucleic acid detection in a pcr by means of a target-sequence-unspecific modular reporter complex
WO2023214843A1 (ko) 2022-05-06 2023-11-09 주식회사 씨젠 핵산 검출 카트리지
KR20250022719A (ko) 2022-06-15 2025-02-17 주식회사 씨젠 기술 문서를 생성하는 방법 및 장치
EP4553489A1 (en) 2022-07-05 2025-05-14 Seegene, Inc. Method for obtaining approximate signal for each of multiple target analytes, and computer device for performing same
US20250336479A1 (en) 2022-07-29 2025-10-30 Seegene, Inc Apparatus and method for providing interface for setting threshold value of parameter
KR20250086619A (ko) 2022-10-14 2025-06-13 주식회사 씨젠 상이한 tm 값을 가지는 2개의 프로브를 이용하여 샘플 내 타겟 핵산을 검출하는 방법
WO2024085731A1 (ko) 2022-10-20 2024-04-25 주식회사 씨젠 타겟 핵산 분자 검출용 시약 개발에 필요한 복수 개의 세부 성능 실험 가이딩 및 실험 결과 기록서 정리 방법
EP4372100A1 (en) * 2022-11-15 2024-05-22 Stilla Technologies Data analysis method for multiplex digital pcr using color-combination
EP4621068A1 (en) 2022-11-16 2025-09-24 Seegene, Inc. Detection of target nucleic acid by quenching-pto cleavage and extension (q-ptoce) assay
WO2024128878A1 (ko) 2022-12-15 2024-06-20 주식회사 씨젠 형광 데이터의 분석 알고리즘에 대한 성능 비교 결과를 디스플레이하는 장치 및 방법
WO2024167289A1 (ko) * 2023-02-07 2024-08-15 주식회사 씨젠 검출 올리고뉴클레오타이드를 이용하여 타겟 올리고뉴클레오타이드를 검출하는 방법
AU2024230052A1 (en) * 2023-02-28 2025-08-14 Seegene, Inc. Detection of target nucleic acid by lpho-assisted pto cleavage and extension assay
CN117802237B (zh) * 2023-10-20 2025-03-21 艾普拜生物科技(苏州)有限公司 信标探针及其组合、用于检测flt3-itd基因突变的pcr扩增试剂及试剂盒

Family Cites Families (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4351760A (en) 1979-09-07 1982-09-28 Syva Company Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5631129A (en) * 1988-12-05 1997-05-20 The Salk Institute For Biological Studies Target nucleic acid amplification/detection systems and methods for the use thereof
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US5965364A (en) 1990-10-09 1999-10-12 Benner; Steven Albert Method for selecting functional deoxyribonucleotide derivatives
US6037120A (en) 1995-10-12 2000-03-14 Benner; Steven Albert Recognition of oligonucleotides containing non-standard base pairs
DK51092D0 (da) 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
US7150982B2 (en) * 1991-09-09 2006-12-19 Third Wave Technologies, Inc. RNA detection assays
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US5541311A (en) * 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
CA2170264A1 (en) 1993-09-10 1995-03-16 Michael W. Konrad Optical detection of position of oligonucleotides on large dna molecules
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US7195871B2 (en) * 1996-01-24 2007-03-27 Third Wave Technologies, Inc Methods and compositions for detecting target sequences
DE69739357D1 (de) 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
DE69719220T2 (de) 1996-11-18 2004-01-22 Takeshi Imanishi Neue nucleotidanaloga
ATE414167T1 (de) 1996-11-29 2008-11-15 Third Wave Tech Inc Fen-1 endonukleasen, gemische und spaltmethoden
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
AU9063398A (en) 1997-09-12 1999-04-05 Exiqon A/S Oligonucleotide analogues
US20060057573A1 (en) 2002-02-15 2006-03-16 Somalogic, Inc Methods and reagents for detecting target binding by nucleic acid ligands
US20100105572A1 (en) * 1997-12-19 2010-04-29 Kris Richard M High throughput assay system
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6656692B2 (en) * 1999-12-21 2003-12-02 Ingeneus Corporation Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US6322980B1 (en) 1999-04-30 2001-11-27 Aclara Biosciences, Inc. Single nucleotide detection using degradation of a fluorescent sequence
JP4724380B2 (ja) 1999-04-20 2011-07-13 独立行政法人産業技術総合研究所 核酸の測定方法に用いる核酸プローブおよびデータを解析する方法
NZ528967A (en) 1999-06-22 2005-03-24 Invitrogen Corp Improved primers and methods for the amplification and discrimination of nucleic acids
US7585632B2 (en) * 1999-10-29 2009-09-08 Hologic, Inc. Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US6893819B1 (en) 2000-11-21 2005-05-17 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US7118860B2 (en) * 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US7381532B2 (en) 1999-10-29 2008-06-03 Stratagene California Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US6265170B1 (en) * 2000-01-24 2001-07-24 Ingeneus Corporation Homogenous assay of duplex of triplex hybridization by means of multiple measurements under varied conditions
US7348141B2 (en) 2000-03-29 2008-03-25 Lgc Limited Hybridization beacon and method of rapid sequence detection and discrimination
AU7125401A (en) 2000-05-19 2001-12-03 David J Marshall Materials and methods for detection of nucleic acids
US7678541B2 (en) 2000-11-21 2010-03-16 Hologic, Inc. Methods and compositions for the detection of a nucleic acid using a non-invasive cleavage reaction
US7309573B2 (en) * 2000-11-21 2007-12-18 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US20030165865A1 (en) * 2001-01-29 2003-09-04 Hinkel Christopher A. Methods of analysis of nucleic acids
WO2002090572A2 (en) * 2001-05-09 2002-11-14 Third Wave Technologies, Inc. Nucleic acid detection in pooled samples
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
WO2003033741A1 (en) 2001-10-16 2003-04-24 Aclara Biosciences, Inc. Universal e-tag primer and probe compositions and methods
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
WO2003089654A2 (en) * 2002-04-22 2003-10-30 Wisconsin Alumni Research Foundation Single nucleotide polymorphism analysis using surface invasive cleavage reactions
US20080187910A1 (en) * 2002-06-26 2008-08-07 Nilsen Thor W Nucleic acid assay and method of detecting the presence of a nucleic acid sequence
CN102930309B (zh) 2002-09-26 2016-03-09 吉田健治 点图形形成方法
US8206904B2 (en) 2002-12-18 2012-06-26 Third Wave Technologies, Inc. Detection of nucleic acids
ES2309304T3 (es) 2003-03-17 2008-12-16 Kenji Yoshida Procedimiento de entrada/salida de informaciones utilizando una plantilla de puntos.
WO2005010199A2 (en) 2003-07-16 2005-02-03 Geneohm Sciences, Inc. Invasive cleavage reaction with electrochemical readout
US20050142595A1 (en) 2003-11-07 2005-06-30 U.S. Genomics, Inc. Intercalator FRET donors or acceptors
JP4851188B2 (ja) 2003-12-19 2012-01-11 日鉄環境エンジニアリング株式会社 核酸測定用新規混合物、及びそれを用いる核酸の新規測定方法並びにそれらに用いる核酸プローブ
CA2497324A1 (en) 2004-02-17 2005-08-17 Affymetrix, Inc. Methods for fragmenting and labelling dna
US20060029954A1 (en) 2004-06-30 2006-02-09 Applera Corporation Compositions and methods for identifying nucleotides in polynucleotide sequences
WO2006004949A1 (en) * 2004-06-30 2006-01-12 Applera Corporation Compositions and methods for detecting and quantitating polynucleotide sequences
KR101243266B1 (ko) 2005-03-05 2013-03-25 주식회사 씨젠 이중 특이성 올리고뉴클레오타이드를 사용한 방법 및 이중 특이성 올리고뉴클레오타이드
CA2609328A1 (en) 2005-05-26 2006-11-30 The Trustees Of Boston University Quantification of nucleic acids and proteins using oligonucleotide mass tags
WO2007011946A2 (en) * 2005-07-15 2007-01-25 Applera Corporation Detection of nucleic acid amplification
US20080193940A1 (en) 2006-12-29 2008-08-14 Applera Corporation, Applied Biosystems Group Systems and methods for detecting nucleic acids
WO2008083261A1 (en) 2006-12-29 2008-07-10 Applera Corporation Systems and methods for detecting nucleic acids
FI20075124A0 (fi) 2007-02-21 2007-02-21 Valtion Teknillinen Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi
KR20090067334A (ko) 2007-12-21 2009-06-25 주식회사 씨젠 핵산 중합효소의 뉴클레아제 활성을 이용한 타깃핵산분자의 검출방법
MX2010010161A (es) * 2008-03-15 2011-03-21 Hologic Inc Star Composiciones y metodos para el analisis de moleculas de acido nucleico durante reacciones de amplificacion.
EP2310522B1 (en) * 2008-06-17 2013-03-20 Sigma-Aldrich Co. LLC Real time polymerase chain reaction process using a universal detection system
CN104673888B (zh) 2008-08-15 2017-09-19 卡斯凯德生物体系股份有限公司 检测核酸
US20110171649A1 (en) * 2008-09-10 2011-07-14 Igor Kutyavin Detection of nucleic acids by oligonucleotide probes cleaved in presence of endonuclease v
US8017327B2 (en) * 2008-10-23 2011-09-13 Honeywell International Inc. Single nucleotide polymorphism genotyping detection via the real-time invader assay microarray platform
CN102216472A (zh) 2008-11-13 2011-10-12 里博克斯艾克斯有限公司 Rna检测方法
EP2256215A1 (en) 2009-04-30 2010-12-01 Steffen Mergemeier Assay system using a nuclease activity of a nucleic acid polymerase
EP2248915B1 (en) 2009-05-05 2013-09-18 Qiagen GmbH Detection of multiple nucleic acid sequences in a reaction cartridge
WO2011027966A2 (en) 2009-09-03 2011-03-10 Seegene, Inc. Td probe and its uses
US9315857B2 (en) * 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
BR112012018394B8 (pt) 2009-12-21 2021-07-27 Seegene Inc método para detecção de uma sequência de ácido nucleico alvo e kit para detecção de uma sequência de ácido nucleico alvo
EP2569449A4 (en) 2010-05-14 2013-12-04 Life Technologies Corp IDENTIFICATION OF NUCLEIC ACIDS
US9921165B2 (en) * 2010-11-05 2018-03-20 Genalyte, Inc. Optical analyte detection systems and methods of use
MX352460B (es) 2011-01-11 2017-11-24 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
JP5852222B2 (ja) 2011-03-29 2016-02-03 シージーン アイエヌシー Ｐｔｏ切断及び延長−依存的切断によるターゲット核酸配列の検出
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출

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