MX2015008494A - Deteccion de secuencia de acidos nucleicos objetivo por ensayo de escision de pto y no hibridacion dependiente de la extension. - Google Patents

Deteccion de secuencia de acidos nucleicos objetivo por ensayo de escision de pto y no hibridacion dependiente de la extension.

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Publication number
MX2015008494A
MX2015008494A MX2015008494A MX2015008494A MX2015008494A MX 2015008494 A MX2015008494 A MX 2015008494A MX 2015008494 A MX2015008494 A MX 2015008494A MX 2015008494 A MX2015008494 A MX 2015008494A MX 2015008494 A MX2015008494 A MX 2015008494A
Authority
MX
Mexico
Prior art keywords
extension
detection
nucleic acid
acid sequence
cto
Prior art date
Application number
MX2015008494A
Other languages
English (en)
Other versions
MX361191B (es
Inventor
Jong Yoon Chun
Young Jo Lee
Original Assignee
Seegene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seegene Inc filed Critical Seegene Inc
Publication of MX2015008494A publication Critical patent/MX2015008494A/es
Publication of MX361191B publication Critical patent/MX361191B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a la detección de una secuencia de ácidos nucleicos objetivo por un ensayo de PCE-NR (Escisión de PTO y No Hibridación Dependiente de la Extensión). La presente invención adopta la ocurrencia de la inhibición de la hibridación entre el HO con el CTO por la formación del dúplex extendido dependiente del objetivo. Por lo tanto, la presente invención puede detectar secuencias objetivo incluso cuando el HO no se escinde. A este respecto, el diseño de la porción de marcado 5´ de secuencias de PTO, CTO y HO se puede realizar fácilmente y las condiciones para las reacciones también se pueden establecer fácilmente. Además, la detección del híbrido entre el CTO y el HO se puede realizar en un recipiente diferente de aquel para la extensión del CTO.
MX2015008494A 2012-12-27 2013-12-27 Detección de secuencia de ácidos nucleicos objetivo por ensayo de escisión de pto y no hibridación dependiente de la extensión. MX361191B (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20120154834 2012-12-27
KR20130008580 2013-01-25
KR20130034670 2013-03-29
PCT/KR2013/012312 WO2014104818A1 (en) 2012-12-27 2013-12-27 Detection of target nucleic acid sequence by pto cleavage and extension-dependent non-hybridization assay

Publications (2)

Publication Number Publication Date
MX2015008494A true MX2015008494A (es) 2015-09-10
MX361191B MX361191B (es) 2018-11-29

Family

ID=51021756

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015008494A MX361191B (es) 2012-12-27 2013-12-27 Detección de secuencia de ácidos nucleicos objetivo por ensayo de escisión de pto y no hibridación dependiente de la extensión.

Country Status (12)

Country Link
US (2) US9783845B2 (es)
EP (1) EP2943586B1 (es)
JP (1) JP6178430B2 (es)
KR (1) KR101760267B1 (es)
CN (1) CN104903465B (es)
AU (1) AU2013371128B2 (es)
BR (1) BR112015014152B1 (es)
CA (1) CA2896213C (es)
ES (1) ES2653642T3 (es)
MX (1) MX361191B (es)
RU (1) RU2620958C2 (es)
WO (1) WO2014104818A1 (es)

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EP3469100B1 (en) 2016-06-10 2021-01-27 Seegene, Inc. Methods for preparing tagging oligonucleotides
KR102301305B1 (ko) 2016-06-30 2021-09-13 주식회사 씨젠 핵산 증폭용 튜브 및 핵산 증폭용 반응 용기
US20190376126A1 (en) * 2016-12-23 2019-12-12 Albert-Ludwigs-Universität Freiburg Two-part mediator probe
KR102279689B1 (ko) 2016-12-29 2021-07-22 주식회사 씨젠 프라이머 다이머 형성을 감소시키고 증폭 효율을 증가시키는 방법
CN108823287B (zh) 2017-04-28 2019-04-12 厦门大学 一种检测靶核酸序列的方法
CN107236815A (zh) * 2017-07-18 2017-10-10 江西贤聚景欣医药生物科技有限公司 用于靶核酸序列检测的多重淬灭荧光探针及方法
US11591645B2 (en) 2017-08-31 2023-02-28 Seegene, Inc. Evaluation of performance of components using a pair of dimer-forming primers
WO2019066461A2 (en) 2017-09-29 2019-04-04 Seegene, Inc. DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY CLEAVAGE ANALYSIS AND EXTENSION OF PTO
EP3735476B1 (en) 2018-01-05 2023-08-23 Seegene, Inc. Method for determining the presence or absence of m. tuberculosis, m. bovis and m. bovis bcg in a sample
EP3899035A1 (en) * 2018-12-20 2021-10-27 F. Hoffmann-La Roche AG Detection of target nucleic acid by solid-phase molography
WO2023043280A1 (ko) 2021-09-17 2023-03-23 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출
EP4265734A1 (de) * 2022-04-22 2023-10-25 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes
WO2023203230A1 (de) * 2022-04-22 2023-10-26 Hahn-Schickard-Gesellschaft Für Angewandte Forschung E. V. Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes
EP4372100A1 (en) * 2022-11-15 2024-05-22 Stilla Technologies Data analysis method for multiplex digital pcr using color-combination

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US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
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KR20020063359A (ko) * 2001-01-27 2002-08-03 일렉트론 바이오 (주) 핵산 및 올리거뉴클레오티드의 상보적 이중결합의 특정서열에 특이적으로 반응하는 절단기법을 이용한 핵산 혼성분석 방법 및 장치
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Also Published As

Publication number Publication date
EP2943586A1 (en) 2015-11-18
JP2016502861A (ja) 2016-02-01
US20160060690A1 (en) 2016-03-03
BR112015014152B1 (pt) 2023-01-24
MX361191B (es) 2018-11-29
AU2013371128B2 (en) 2017-08-10
BR112015014152A2 (es) 2017-08-22
RU2015130335A (ru) 2017-01-30
EP2943586A4 (en) 2016-09-28
JP6178430B2 (ja) 2017-08-09
CN104903465A (zh) 2015-09-09
KR20150093766A (ko) 2015-08-18
CN104903465B (zh) 2017-04-26
AU2013371128A1 (en) 2015-08-13
US9783845B2 (en) 2017-10-10
WO2014104818A1 (en) 2014-07-03
CA2896213A1 (en) 2014-07-03
KR101760267B1 (ko) 2017-07-24
US20180057869A1 (en) 2018-03-01
CA2896213C (en) 2018-12-11
RU2620958C2 (ru) 2017-05-30
EP2943586B1 (en) 2017-09-20
ES2653642T3 (es) 2018-02-08

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