EA201391723A1 - Продукты и способы для идентификации мультиплексных нуклеиновых кислот - Google Patents

Продукты и способы для идентификации мультиплексных нуклеиновых кислот

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Publication number
EA201391723A1
EA201391723A1 EA201391723A EA201391723A EA201391723A1 EA 201391723 A1 EA201391723 A1 EA 201391723A1 EA 201391723 A EA201391723 A EA 201391723A EA 201391723 A EA201391723 A EA 201391723A EA 201391723 A1 EA201391723 A1 EA 201391723A1
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Eurasian Patent Office
Prior art keywords
nucleic acids
oligonucleotides
absence
products
methods
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EA201391723A
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English (en)
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EA027558B1 (ru
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Кристиане Хониш
Дирк Йоханнес Ван Ден Бом
Майкл Моско
Андерс Нюгрен
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Секвеном, Инк.
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Application filed by Секвеном, Инк. filed Critical Секвеном, Инк.
Publication of EA201391723A1 publication Critical patent/EA201391723A1/ru
Publication of EA027558B1 publication Critical patent/EA027558B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

Здесь обеспечены продукты и процессы для детектирования присутствия или отсутствия множественных нуклеиновых кислот-мишеней. Некоторые способы включают амплификацию нуклеиновых кислот-мишеней или их части; удлинение олигонуклеотидов, которые специфически гибридизируются с этими ампликонами, где эти удлиненные олигонуклеотиды включают захватывающий агент; захватывание удлиненных олигонуклеотидов к твердой фазе через этот захватывающий агент; высвобождение удлиненных олигонуклеотидов конкуренцией с конкурирующим агентом; детектирование этого удлиненного олигонуклеотида и определение посредством этого присутствия или отсутствия каждой нуклеиновой кислоты-мишени по присутствию или отсутствию этих удлиненных олигонуклеотидов.
EA201391723A 2011-05-19 2012-05-18 Способ обнаружения мультиплексных нуклеиновых кислот EA027558B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161488082P 2011-05-19 2011-05-19
PCT/US2012/038710 WO2012159089A1 (en) 2011-05-19 2012-05-18 Products and processes for multiplex nucleic acid identification

Publications (2)

Publication Number Publication Date
EA201391723A1 true EA201391723A1 (ru) 2014-06-30
EA027558B1 EA027558B1 (ru) 2017-08-31

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Family Applications (1)

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EA201391723A EA027558B1 (ru) 2011-05-19 2012-05-18 Способ обнаружения мультиплексных нуклеиновых кислот

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US (4) US10604791B2 (ru)
EP (1) EP2710144B1 (ru)
JP (2) JP6040227B2 (ru)
CN (2) CN103717751A (ru)
AU (1) AU2012254985C1 (ru)
CA (1) CA2835942C (ru)
EA (1) EA027558B1 (ru)
WO (1) WO2012159089A1 (ru)

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