EA201491821A1 - Модифицированные олигонуклеотиды, включающие тиольные функциональные группы, и их применение для детекции нуклеиновых кислот - Google Patents

Модифицированные олигонуклеотиды, включающие тиольные функциональные группы, и их применение для детекции нуклеиновых кислот

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Publication number
EA201491821A1
EA201491821A1 EA201491821A EA201491821A EA201491821A1 EA 201491821 A1 EA201491821 A1 EA 201491821A1 EA 201491821 A EA201491821 A EA 201491821A EA 201491821 A EA201491821 A EA 201491821A EA 201491821 A1 EA201491821 A1 EA 201491821A1
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EA
Eurasian Patent Office
Prior art keywords
functional groups
modified oligonucleotides
detection
carbon
thiol functional
Prior art date
Application number
EA201491821A
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English (en)
Other versions
EA029907B1 (ru
Inventor
Шанталь Фурнье-Вирт
Мириам Леро
Жан-Франсуа Канталуб
Жан-Жак Вассер
Франсуа Морван
Альбер Мейер
Жюли Майен
Кароль Ше
Кароль Фарр
Original Assignee
Этаблиссман Франсе Дю Сан
Сантр Насьональ Де Ля Решерш Сьентифик (Снрс)
Юниверсите Де Монпелье 1
Юниверсите Клод Бернар Лион 1
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Application filed by Этаблиссман Франсе Дю Сан, Сантр Насьональ Де Ля Решерш Сьентифик (Снрс), Юниверсите Де Монпелье 1, Юниверсите Клод Бернар Лион 1 filed Critical Этаблиссман Франсе Дю Сан
Publication of EA201491821A1 publication Critical patent/EA201491821A1/ru
Publication of EA029907B1 publication Critical patent/EA029907B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Communicable Diseases (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Изобретение относится к модифицированным олигонуклеотидам, имеющим две или более тиольных функциональных группы, которые могут быть зафиксированы на золотой поверхности или на привитой поверхности, в частности на поверхности, включающей по меньшей мере одну углерод-углеродную двойную связь, или углерод-углеродную тройную связь, или галогенацетамидные группы, предпочтительно малеимидные или акриламидные функциональные группы. Изобретение также относится к способу детекции нуклеиновой кислоты в биологическом образце, включающему стадию детекции гибридизации между модифицированным олигонуклеотидом и нуклеиновой кислотой-мишенью, амплифицированной из биологического образца. Изобретение относится, в частности, к способу детекции, генотипирования или секвенирования патогенного организма, предпочтительно вируса.
EA201491821A 2012-04-04 2013-04-04 Модифицированные олигонуклеотиды, включающие тиольные функциональные группы, и их применение для детекции нуклеиновых кислот EA029907B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1253122A FR2989089B1 (fr) 2012-04-04 2012-04-04 Oligonucleotides modifies comprenant des fonctions thiol et leur utilisation pour la detection d'acides nucleiques
PCT/EP2013/057150 WO2013150122A1 (fr) 2012-04-04 2013-04-04 Oligonucleotides modifies compenant des fonctions thiol et leur utilisation pour la detection d'acides nucleiques

Publications (2)

Publication Number Publication Date
EA201491821A1 true EA201491821A1 (ru) 2015-02-27
EA029907B1 EA029907B1 (ru) 2018-05-31

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EA201491821A EA029907B1 (ru) 2012-04-04 2013-04-04 Модифицированные олигонуклеотиды, включающие тиольные функциональные группы, и их применение для детекции нуклеиновых кислот

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US (2) US20150232957A1 (ru)
EP (1) EP2859008B1 (ru)
JP (1) JP6175489B2 (ru)
KR (1) KR102186143B1 (ru)
AU (1) AU2013244900B2 (ru)
BR (1) BR112014024601B1 (ru)
CA (1) CA2868926C (ru)
EA (1) EA029907B1 (ru)
ES (1) ES2624993T3 (ru)
FR (1) FR2989089B1 (ru)
IN (1) IN2014DN08170A (ru)
WO (1) WO2013150122A1 (ru)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017218293A1 (en) 2016-06-16 2017-12-21 Richard Edward Watts Oligonucleotide directed and recorded combinatorial synthesis of encoded probe molecules
EP3619340A4 (en) 2017-05-02 2021-01-20 Haystack Sciences Corporation MOLECULES FOR THE VERIFICATION OF OLIGONUCLEOTIDE DIRECTED COMBINATIONAL SYNTHESIS AND METHOD OF MANUFACTURING AND USING THEREOF
US11236334B2 (en) * 2017-08-22 2022-02-01 National University Corporation Nagoya University Modified polynucleotide
US20200263163A1 (en) * 2017-09-25 2020-08-20 Haystack Sciences Corporation Multinomial encoding for oligonucleotide-directed combinatorial chemistry
US20240182990A1 (en) 2021-03-09 2024-06-06 Etablissement Français Du Sang Detection of infectious agent based on recombinase polymerase amplification combined with a magnetic field-enhanced agglutination

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6041183A (ja) * 1983-08-16 1985-03-04 三菱電機株式会社 入場管理装置
CA2073846C (en) * 1991-07-15 2007-09-18 David S. Jones Modified phosphorous intermediates for providing functional groups on the 5' end of oligonucleotides
JPH0641183A (ja) * 1992-07-23 1994-02-15 Mitsubishi Kasei Corp オリゴヌクレオチド単分子膜
DE10163836A1 (de) * 2001-12-22 2003-07-10 Friz Biochem Gmbh Multifunktionales Reagenz zur Synthese von thiolmodifizierten Oligomeren
US7371585B2 (en) * 2003-12-31 2008-05-13 Genencor International, Inc. Membranes incorporating recognition moieties
US7167395B1 (en) 2005-07-13 2007-01-23 Infineon Technologies Flash Gmbh & Co. Kg Non-volatile semiconductor memory
CN101341247A (zh) 2005-12-23 2009-01-07 西门子医疗保健诊断公司 对hcv进行基因分型的方法和试剂
FR2989086B1 (fr) 2012-04-04 2017-03-24 Centre Nat De La Rech Scient (Cnrs) Composes thiol et leur utilisation pour la synthese d'oligonucleotides modifies

Also Published As

Publication number Publication date
EA029907B1 (ru) 2018-05-31
KR102186143B1 (ko) 2020-12-03
KR20150021493A (ko) 2015-03-02
ES2624993T3 (es) 2017-07-18
JP2015514402A (ja) 2015-05-21
CA2868926C (fr) 2021-05-04
US11390643B2 (en) 2022-07-19
FR2989089B1 (fr) 2020-02-07
EP2859008B1 (fr) 2017-02-22
AU2013244900A1 (en) 2014-10-16
EP2859008A1 (fr) 2015-04-15
US20190376151A1 (en) 2019-12-12
AU2013244900B2 (en) 2017-01-12
US20150232957A1 (en) 2015-08-20
FR2989089A1 (fr) 2013-10-11
CA2868926A1 (fr) 2013-10-10
BR112014024601B1 (pt) 2022-05-24
BR112014024601A2 (pt) 2018-04-17
WO2013150122A1 (fr) 2013-10-10
IN2014DN08170A (ru) 2015-05-01
JP6175489B2 (ja) 2017-08-02

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