AU5296496A - Mutation detection by differential primer extension of mutant and wildtype target sequences - Google Patents

Mutation detection by differential primer extension of mutant and wildtype target sequences

Info

Publication number
AU5296496A
AU5296496A AU52964/96A AU5296496A AU5296496A AU 5296496 A AU5296496 A AU 5296496A AU 52964/96 A AU52964/96 A AU 52964/96A AU 5296496 A AU5296496 A AU 5296496A AU 5296496 A AU5296496 A AU 5296496A
Authority
AU
Australia
Prior art keywords
mutant
target sequences
primer extension
mutation detection
wildtype target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU52964/96A
Inventor
Eoin D. Fahy
Soumitra Ghosh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Migenix Corp
Original Assignee
Mitokor Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitokor Inc filed Critical Mitokor Inc
Publication of AU5296496A publication Critical patent/AU5296496A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
AU52964/96A 1995-03-24 1996-02-14 Mutation detection by differential primer extension of mutant and wildtype target sequences Abandoned AU5296496A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US41065895A 1995-03-24 1995-03-24
US410658 1995-03-24
PCT/US1996/002045 WO1996030545A1 (en) 1995-03-24 1996-02-14 Mutation detection by differential primer extension of mutant and wildtype target sequences

Publications (1)

Publication Number Publication Date
AU5296496A true AU5296496A (en) 1996-10-16

Family

ID=23625669

Family Applications (1)

Application Number Title Priority Date Filing Date
AU52964/96A Abandoned AU5296496A (en) 1995-03-24 1996-02-14 Mutation detection by differential primer extension of mutant and wildtype target sequences

Country Status (2)

Country Link
AU (1) AU5296496A (en)
WO (1) WO1996030545A1 (en)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US6027883A (en) * 1994-03-30 2000-02-22 Mitokor Optimal procedure for isolation of mutant mitochondrial alleles
US5885775A (en) * 1996-10-04 1999-03-23 Perseptive Biosystems, Inc. Methods for determining sequences information in polynucleotides using mass spectrometry
US5888778A (en) * 1997-06-16 1999-03-30 Exact Laboratories, Inc. High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers
US6566101B1 (en) 1997-06-16 2003-05-20 Anthony P. Shuber Primer extension methods for detecting nucleic acids
CA2325854C (en) 1998-04-22 2005-12-06 Enterprise Ireland Trading As Bioresearch Ireland A method for the characterisation of nucleic acid molecules involving generation of extendible upstream dna fragments resulting from the cleavage of nucleic acid at an abasic site
CA2245039A1 (en) * 1998-08-13 2000-02-13 Vito Scalia Primer-specific and mispair extension assay for identifying gene variation
US6150105A (en) * 1998-08-20 2000-11-21 Genetic Assays, Inc. Methods of screening nucleic acids for nucleotide variations
AU5923099A (en) * 1998-09-16 2000-04-03 Glaxo Group Limited Multiple sequencing method
WO2000031298A1 (en) * 1998-11-23 2000-06-02 Exact Laboratories, Inc. Methods for detecting lower-frequency molecules
US6280947B1 (en) * 1999-08-11 2001-08-28 Exact Sciences Corporation Methods for detecting nucleotide insertion or deletion using primer extension
US6503718B2 (en) * 1999-01-10 2003-01-07 Exact Sciences Corporation Methods for detecting mutations using primer extension for detecting disease
EP1198597A1 (en) * 1999-07-22 2002-04-24 Artus Gesellschaft Für Molekularbiologische Diagnostik und Entwicklung MbH. Method for the species-specific detection of organisms
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
JP2001245698A (en) * 1999-11-22 2001-09-11 Xiao Bing Wang Method for detecting nucleic acid
US6762018B1 (en) * 1999-12-23 2004-07-13 Tetragen Sa Analysis of nucleotide polymorphisms at a site
US20020018999A1 (en) * 2000-02-24 2002-02-14 Jan Henriksson Methods for characterizing polymorphisms
US6824980B2 (en) 2000-06-08 2004-11-30 Xiao Bing Wang Isometric primer extension method and kit for detection and quantification of specific nucleic acid
SG115374A1 (en) * 2000-07-17 2005-10-28 Xiao Bing Wang Detection of sequence variation of nucleic acid by shifted termination analysis
US7148343B2 (en) * 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
EP1721014B1 (en) * 2004-02-18 2013-07-17 Trustees Of Boston University Method for detecting and quantifying rare mutations/polymorphisms
WO2005111244A2 (en) * 2004-05-10 2005-11-24 Exact Sciences Corporation Methods for detecting a mutant nucleic acid
WO2006047787A2 (en) 2004-10-27 2006-05-04 Exact Sciences Corporation Method for monitoring disease progression or recurrence
US9777314B2 (en) 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
EP2152915A2 (en) * 2007-05-14 2010-02-17 Insight Genetics, Inc. Methods of screening nucleic acids for single nucleotide variations
WO2012159089A1 (en) 2011-05-19 2012-11-22 Sequenom, Inc. Products and processes for multiplex nucleic acid identification
AU2016250849A1 (en) 2015-04-24 2017-11-02 Agena Bioscience, Inc. Multiplex methods for detection and quantification of minor variants
US10233489B2 (en) 2015-04-24 2019-03-19 Agena Bioscience, Inc. Multiplexed method for the identification and quantitation of minor alleles and polymorphisms
CN114250296B (en) * 2022-02-09 2024-02-20 深圳市妇幼保健院 Primer group for detecting Leber hereditary optic neuropathy based on nucleic acid mass spectrum

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE66572B1 (en) * 1989-02-13 1996-01-24 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
FR2650840B1 (en) * 1989-08-11 1991-11-29 Bertin & Cie RAPID DETECTION AND / OR IDENTIFICATION OF A SINGLE BASED ON A NUCLEIC ACID SEQUENCE, AND ITS APPLICATIONS
CA2186636A1 (en) * 1994-03-30 1995-10-12 Corinna Herrnstadt Diagnosis, therapy and cellular and animal models for diseases associated with mitochondrial defects

Also Published As

Publication number Publication date
WO1996030545A1 (en) 1996-10-03

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