MX361191B - Detección de secuencia de ácidos nucleicos objetivo por ensayo de escisión de pto y no hibridación dependiente de la extensión. - Google Patents

Detección de secuencia de ácidos nucleicos objetivo por ensayo de escisión de pto y no hibridación dependiente de la extensión.

Info

Publication number
MX361191B
MX361191B MX2015008494A MX2015008494A MX361191B MX 361191 B MX361191 B MX 361191B MX 2015008494 A MX2015008494 A MX 2015008494A MX 2015008494 A MX2015008494 A MX 2015008494A MX 361191 B MX361191 B MX 361191B
Authority
MX
Mexico
Prior art keywords
extension
detection
nucleic acid
acid sequence
cto
Prior art date
Application number
MX2015008494A
Other languages
English (en)
Other versions
MX2015008494A (es
Inventor
Jong Yoon Chun
Young Jo Lee
Original Assignee
Seegene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seegene Inc filed Critical Seegene Inc
Publication of MX2015008494A publication Critical patent/MX2015008494A/es
Publication of MX361191B publication Critical patent/MX361191B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a la detección de una secuencia de ácidos nucleicos objetivo por un ensayo de PCE-NR (Escisión de PTO y No Hibridación Dependiente de la Extensión). La presente invención adopta la ocurrencia de la inhibición de la hibridación entre el HO con el CTO por la formación del dúplex extendido dependiente del objetivo. Por lo tanto, la presente invención puede detectar secuencias objetivo incluso cuando el HO no se escinde. A este respecto, el diseño de la porción de marcado 5´ de secuencias de PTO, CTO y HO se puede realizar fácilmente y las condiciones para las reacciones también se pueden establecer fácilmente. Además, la detección del híbrido entre el CTO y el HO se puede realizar en un recipiente diferente de aquel para la extensión del CTO.
MX2015008494A 2012-12-27 2013-12-27 Detección de secuencia de ácidos nucleicos objetivo por ensayo de escisión de pto y no hibridación dependiente de la extensión. MX361191B (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20120154834 2012-12-27
KR20130008580 2013-01-25
KR20130034670 2013-03-29
PCT/KR2013/012312 WO2014104818A1 (en) 2012-12-27 2013-12-27 Detection of target nucleic acid sequence by pto cleavage and extension-dependent non-hybridization assay

Publications (2)

Publication Number Publication Date
MX2015008494A MX2015008494A (es) 2015-09-10
MX361191B true MX361191B (es) 2018-11-29

Family

ID=51021756

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015008494A MX361191B (es) 2012-12-27 2013-12-27 Detección de secuencia de ácidos nucleicos objetivo por ensayo de escisión de pto y no hibridación dependiente de la extensión.

Country Status (12)

Country Link
US (2) US9783845B2 (es)
EP (1) EP2943586B1 (es)
JP (1) JP6178430B2 (es)
KR (1) KR101760267B1 (es)
CN (1) CN104903465B (es)
AU (1) AU2013371128B2 (es)
BR (1) BR112015014152B1 (es)
CA (1) CA2896213C (es)
ES (1) ES2653642T3 (es)
MX (1) MX361191B (es)
RU (1) RU2620958C2 (es)
WO (1) WO2014104818A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11066704B2 (en) 2016-06-10 2021-07-20 Seegene, Inc. Methods for preparing tagging oligonucleotides
KR102264865B1 (ko) 2016-06-30 2021-06-14 주식회사 씨젠 반응 분석용 형광 검출 장치
BR112019012925A2 (pt) * 2016-12-23 2019-12-10 Albert-Ludwigs-Universität Freiburg sonda mediadora em duas partes
ES2972231T3 (es) 2016-12-29 2024-06-11 Seegene Inc Procedimiento para reducir la formación de dímeros de cebadores y aumentar la eficacia de la amplificación
CN108823287B (zh) 2017-04-28 2019-04-12 厦门大学 一种检测靶核酸序列的方法
CN107236815A (zh) * 2017-07-18 2017-10-10 江西贤聚景欣医药生物科技有限公司 用于靶核酸序列检测的多重淬灭荧光探针及方法
WO2019045532A2 (en) 2017-08-31 2019-03-07 Seegene, Inc. EVALUATION OF COMPONENT PERFORMANCE USING A PAIR OF DIMER FORMER PRIMERS
WO2019066461A2 (en) 2017-09-29 2019-04-04 Seegene, Inc. DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY CLEAVAGE ANALYSIS AND EXTENSION OF PTO
EP3735476B1 (en) 2018-01-05 2023-08-23 Seegene, Inc. Method for determining the presence or absence of m. tuberculosis, m. bovis and m. bovis bcg in a sample
CN113195734A (zh) * 2018-12-20 2021-07-30 豪夫迈·罗氏有限公司 通过固相分子成像检测靶核酸
WO2023043280A1 (ko) 2021-09-17 2023-03-23 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출
EP4265734A1 (de) * 2022-04-22 2023-10-25 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes
WO2023203230A1 (de) * 2022-04-22 2023-10-26 Hahn-Schickard-Gesellschaft Für Angewandte Forschung E. V. Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes
EP4372100A1 (en) * 2022-11-15 2024-05-22 Stilla Technologies Data analysis method for multiplex digital pcr using color-combination

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US5541311A (en) 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
US7381532B2 (en) 1999-10-29 2008-06-03 Stratagene California Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US6893819B1 (en) 2000-11-21 2005-05-17 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7309573B2 (en) 2000-11-21 2007-12-18 Stratagene California Methods for detection of a nucleic acid by sequential amplification
KR20020063359A (ko) 2001-01-27 2002-08-03 일렉트론 바이오 (주) 핵산 및 올리거뉴클레오티드의 상보적 이중결합의 특정서열에 특이적으로 반응하는 절단기법을 이용한 핵산 혼성분석 방법 및 장치
GB0205455D0 (en) * 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
CN101076537B (zh) * 2003-11-19 2012-07-04 阿莱洛吉克生物科学公司 标记有多个荧光团的寡核苷酸
WO2008011004A2 (en) * 2006-07-17 2008-01-24 Brandeis University Specialized oligonucleotides and their use in nucleic acid amplification and detection
US20090023151A1 (en) 2006-08-30 2009-01-22 Dawson Elliott P Method For The Labeling And Detection Of Small Polynucleotides
US20080241838A1 (en) 2006-12-29 2008-10-02 Applera Corporation, Applied Biosystems Group Methods and systems for detecting nucleic acids
WO2012096430A1 (en) * 2011-01-11 2012-07-19 Seegene, Inc. Detection of target nucleic acid sequences by pto cleavage and extension assay
US11078525B2 (en) * 2011-03-29 2021-08-03 Seegene, Inc. Detection of target nucleic acid sequence by PTO cleavage and extension-dependent cleavage
MX342067B (es) * 2011-05-04 2016-09-09 Seegene Inc Detección de secuencias de ácido nucleico objetivo por desdoblamiento e hibridización de oligonucleótido de sonda.

Also Published As

Publication number Publication date
AU2013371128A1 (en) 2015-08-13
AU2013371128B2 (en) 2017-08-10
CN104903465B (zh) 2017-04-26
JP2016502861A (ja) 2016-02-01
WO2014104818A1 (en) 2014-07-03
JP6178430B2 (ja) 2017-08-09
BR112015014152A2 (es) 2017-08-22
EP2943586B1 (en) 2017-09-20
MX2015008494A (es) 2015-09-10
US20160060690A1 (en) 2016-03-03
RU2620958C2 (ru) 2017-05-30
EP2943586A1 (en) 2015-11-18
ES2653642T3 (es) 2018-02-08
CN104903465A (zh) 2015-09-09
CA2896213A1 (en) 2014-07-03
CA2896213C (en) 2018-12-11
KR20150093766A (ko) 2015-08-18
KR101760267B1 (ko) 2017-07-24
US20180057869A1 (en) 2018-03-01
BR112015014152B1 (pt) 2023-01-24
US9783845B2 (en) 2017-10-10
RU2015130335A (ru) 2017-01-30
EP2943586A4 (en) 2016-09-28

Similar Documents

Publication Publication Date Title
MX2015008494A (es) Deteccion de secuencia de acidos nucleicos objetivo por ensayo de escision de pto y no hibridacion dependiente de la extension.
MY174052A (en) Detection of target nucleic acid sequences by pto cleavage and extension assay
MY157531A (en) Td probe and its uses
ZA201405463B (en) Detection of target nucleic acid sequence by pto cleavage and extension-dependent signaling oligonucleotide hybridization assay
WO2012134195A3 (en) Detection of target nucleic acid sequence by pto cleavage and extension-dependent cleavage
EA201391723A1 (ru) Продукты и способы для идентификации мультиплексных нуклеиновых кислот
SG194722A1 (en) Probe based nucleic acid detection
EP4299760A3 (en) Probes for improved melt discrimination and multiplexing in nucleic acid assays
PH12015500430A1 (en) Method for screening cancer
MX342067B (es) Detección de secuencias de ácido nucleico objetivo por desdoblamiento e hibridización de oligonucleótido de sonda.
WO2016154337A3 (en) Method for identification and enumeration of nucleic acid sequences
MX354465B (es) Deteccion de variacion del nucleotido en la secuencia de acido nucleico de objetivo por ensayo de desdoblamiento y extension de pto.
NZ706930A (en) Genetic markers for myb28
EA201171131A1 (ru) Усовершенствованное определение экспрессии генов
JP2014500729A5 (es)
WO2011003690A8 (en) Highly sensitive rapid isothermal method for the detection of point mutations and snps, a set of primers and a kit therefor
EP2450443A4 (en) METHOD FOR AMPLIFYING TARGET SEQUENCES, METHOD FOR DETECTION OF POLYMORPHISM, AND REAGENTS SUITABLE FOR THESE PROCESSES
WO2010083046A3 (en) Methods for using next generation sequencing to identify 5-methyl cytosines in the genome
MX2016009008A (es) Metodo para probar un gen mutante a traves de reaccion en cadena de la polimerasa en tiempo real usando adn polimerasa con actividad inhibida de endonucleasa de solapa 5´.
WO2015057008A3 (en) Detection of target nucleic acid sequence on solid phase by pto cleavage and extension using hcto assay
MY158590A (en) A diagnostic system for malaria
WO2012073494A3 (en) Sugarcane-stalk-diameter-related marker and the use thereof
TH124375A (th) โพรบ td และประโยชน์ของมัน
TH124375B (th) โพรบ td และประโยชน์ของมัน
UA85434U (ru) СПОСОБ ОПРЕДЕЛЕНИЯ ОДНОНУКЛЕОТИДНОГО ПОЛИМОРФИЗМА g28197A>G ГЕНА ЭЛАСТИНА МЕТОДОМ АЛЛЕЛЬ-СПЕЦИФИЧЕСКОЙ ПОЛИМЕРАЗНОЙ ЦЕПНОЙ РЕАКЦИИ

Legal Events

Date Code Title Description
FG Grant or registration