BR112016017228A2 - Métodos e kits para detectar ácidos nucleicos que são exclusivos para o evento dp-032218-9 e para a determinação da zigosidade de um ácido nucleico exclusivo para o evento dp-032218-9 - Google Patents

Métodos e kits para detectar ácidos nucleicos que são exclusivos para o evento dp-032218-9 e para a determinação da zigosidade de um ácido nucleico exclusivo para o evento dp-032218-9

Info

Publication number
BR112016017228A2
BR112016017228A2 BR112016017228A BR112016017228A BR112016017228A2 BR 112016017228 A2 BR112016017228 A2 BR 112016017228A2 BR 112016017228 A BR112016017228 A BR 112016017228A BR 112016017228 A BR112016017228 A BR 112016017228A BR 112016017228 A2 BR112016017228 A2 BR 112016017228A2
Authority
BR
Brazil
Prior art keywords
event
exclusive
kits
maize
zygosity
Prior art date
Application number
BR112016017228A
Other languages
English (en)
Inventor
Beatty Mary
Brink Ken
Cox Crane Virginia
Diehn Scott
Laurence Lu Albert
J Young Gregory
Original Assignee
Pioneer Hi Bred Int
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2014/013007 external-priority patent/WO2014116989A1/en
Application filed by Pioneer Hi Bred Int, Du Pont filed Critical Pioneer Hi Bred Int
Publication of BR112016017228A2 publication Critical patent/BR112016017228A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

MÉTODOS E KITS PARA DETECTAR ÁCIDOS NUCLEICOS QUE SÃO EXCLUSIVOS PARA O EVENTO DP-032218-9 E PARA A DETERMINAÇÃO DA ZIGOSIDADE DE UM ÁCIDO NUCLEICO EXCLUSIVO PARA O EVENTO DP-032218-9. A revelação fornece composições de DNA que se referem a plantas de milho maís resistentes a insetos transgênicas. São também fornecidos ensaios para detectar a presença do evento de milho maís DP-032218-9 com base na sequência de DNA do construto recombinante inserido no genoma de milho maís e as sequências de DNA que flanqueiam o sítio de inserção. São fornecidos kits e condições úteis para conduzir os ensaios.
BR112016017228A 2013-01-25 2014-04-01 Métodos e kits para detectar ácidos nucleicos que são exclusivos para o evento dp-032218-9 e para a determinação da zigosidade de um ácido nucleico exclusivo para o evento dp-032218-9 BR112016017228A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361756897P 2013-01-25 2013-01-25
PCT/US2014/013007 WO2014116989A1 (en) 2013-01-25 2014-01-24 Maize event dp-032218-9 and methods for detection thereof
PCT/US2014/032526 WO2015112182A1 (en) 2013-01-25 2014-04-01 Maize event dp-032218-9 and methods for detection thereof

Publications (1)

Publication Number Publication Date
BR112016017228A2 true BR112016017228A2 (pt) 2017-10-03

Family

ID=50680190

Family Applications (2)

Application Number Title Priority Date Filing Date
BR112015017788A BR112015017788A2 (pt) 2013-01-25 2014-01-24 Construto de dna, método para produzir uma planta, método de detecção da presença de uma molécula de ácido nucleico, par de iniciadores de polinucleotídio, evento de milho, método de detecção da presença do dna e kit para detectar ácidos nucleicos
BR112016017228A BR112016017228A2 (pt) 2013-01-25 2014-04-01 Métodos e kits para detectar ácidos nucleicos que são exclusivos para o evento dp-032218-9 e para a determinação da zigosidade de um ácido nucleico exclusivo para o evento dp-032218-9

Family Applications Before (1)

Application Number Title Priority Date Filing Date
BR112015017788A BR112015017788A2 (pt) 2013-01-25 2014-01-24 Construto de dna, método para produzir uma planta, método de detecção da presença de uma molécula de ácido nucleico, par de iniciadores de polinucleotídio, evento de milho, método de detecção da presença do dna e kit para detectar ácidos nucleicos

Country Status (2)

Country Link
BR (2) BR112015017788A2 (pt)
WO (1) WO2015112182A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6873979B2 (ja) 2015-09-11 2021-05-19 バイエル・クロップサイエンス・アクチェンゲゼルシャフト Ｈｐｐｄ変異体および使用方法
CN112301145A (zh) * 2020-10-13 2021-02-02 中国农业科学院生物技术研究所 转基因水稻eb7001s-6的rpa检测引物与探针组合、试剂盒及检测方法
CN112301146A (zh) * 2020-10-13 2021-02-02 中国农业科学院生物技术研究所 转基因水稻b2a68-1的rpa检测引物与探针组合、试剂盒及检测方法
CN113025724B (zh) * 2021-03-10 2023-01-17 华南农业大学 一种鉴定小圆胸小蠹昆虫的双重pcr引物、方法及试剂盒

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1942582A (zh) * 2004-02-25 2007-04-04 先锋高级育种国际公司 新的苏云金芽孢杆菌晶体多肽、多核苷酸及其组合物
US8729336B2 (en) * 2009-01-23 2014-05-20 Pioneer Hi-Bred International, Inc Protein mixtures for maize insect control
WO2011075595A1 (en) * 2009-12-17 2011-06-23 Pioneer Hi-Bred International, Inc. Maize event dp-043a47-3 and methods for detection thereof
US20110151441A1 (en) * 2009-12-18 2011-06-23 Dow Agrosciences Llc Endpoint taqman methods for determining zygosity of corn comprising tc1507 events

Also Published As

Publication number Publication date
BR112015017788A2 (pt) 2017-08-22
WO2015112182A1 (en) 2015-07-30

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements
B350 Update of information on the portal [chapter 15.35 patent gazette]