BR112014018940A8 - Método e kit para detecção de uma sequência de ácido nucleico alvo - Google Patents

Método e kit para detecção de uma sequência de ácido nucleico alvo

Info

Publication number
BR112014018940A8
BR112014018940A8 BR112014018940A BR112014018940A BR112014018940A8 BR 112014018940 A8 BR112014018940 A8 BR 112014018940A8 BR 112014018940 A BR112014018940 A BR 112014018940A BR 112014018940 A BR112014018940 A BR 112014018940A BR 112014018940 A8 BR112014018940 A8 BR 112014018940A8
Authority
BR
Brazil
Prior art keywords
nucleic acid
present
kit
detecting
acid sequence
Prior art date
Application number
BR112014018940A
Other languages
English (en)
Other versions
BR112014018940A2 (pt
BR112014018940B1 (pt
Inventor
Yoon Chun Joug
Jo Lee Young
Original Assignee
Seegene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seegene Inc filed Critical Seegene Inc
Publication of BR112014018940A2 publication Critical patent/BR112014018940A2/pt
Publication of BR112014018940A8 publication Critical patent/BR112014018940A8/pt
Publication of BR112014018940B1 publication Critical patent/BR112014018940B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

DETECÇÃO DE SEQUÊNCIA-ALVO DE ÁCIDO NUCLÉICO POR CLIVAGEM DE PTO E ENSAIO DE HIBRIDIZAÇÃO DE OLIGONUCLEOTÍDEO DE SINALIZAÇÃO DEPENDENTE DE EXTENSÃO A presente invenção refere-se à detecção de uma sequência-alvo de ácido nucléico por ensaio de PCE-SH (clivagem de PTO e Hibridização de Oligonucleotídeo de Sinalização Dependente de Extensão). A presente invenção não usa sondas a serem hibridizadas com sequências-alvo de ácido nucléico para provisão de sinais-alvo. Interessantemente, a presente invenção usa sondas (oligonucleotídeos de sinalização) a serem hibridizadas com trançado estendido formado em uma maneira dependente do alvo em que o trançado estendido é sintetizado usando o CTO artificialmente selecionado como modelo.
BR112014018940-4A 2012-02-02 2012-07-03 Método e kit para detecção de uma sequência de ácido nucleico alvo BR112014018940B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
KR10-2012-0010681 2012-02-02
KR20120010681 2012-02-02
KR1020120028429A KR20130101952A (ko) 2012-02-02 2012-03-20 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
KR10-2012-0028429 2012-03-20
PCT/KR2012/005281 WO2013115442A1 (en) 2012-02-02 2012-07-03 Detection of target nucleic acid sequence by pto cleavage and extension-dependent signaling oligonucleotide hybridization assay

Publications (3)

Publication Number Publication Date
BR112014018940A2 BR112014018940A2 (pt) 2017-06-20
BR112014018940A8 true BR112014018940A8 (pt) 2017-07-11
BR112014018940B1 BR112014018940B1 (pt) 2021-03-23

Family

ID=49451920

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014018940-4A BR112014018940B1 (pt) 2012-02-02 2012-07-03 Método e kit para detecção de uma sequência de ácido nucleico alvo

Country Status (15)

Country Link
US (2) US9868980B2 (pt)
EP (1) EP2809797B1 (pt)
JP (1) JP6005180B2 (pt)
KR (2) KR20130101952A (pt)
CN (2) CN106011234B (pt)
AU (2) AU2012368436B2 (pt)
BR (1) BR112014018940B1 (pt)
CA (1) CA2862488C (pt)
IL (1) IL233773B (pt)
MX (1) MX361903B (pt)
NO (1) NO20140948A1 (pt)
RU (1) RU2608501C2 (pt)
SG (1) SG11201404349YA (pt)
WO (1) WO2013115442A1 (pt)
ZA (1) ZA201405463B (pt)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX340258B (es) 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
US11078525B2 (en) 2011-03-29 2021-08-03 Seegene, Inc. Detection of target nucleic acid sequence by PTO cleavage and extension-dependent cleavage
US9850524B2 (en) 2011-05-04 2017-12-26 Seegene, Inc. Detection of target nucleic acid sequences by PO cleavage and hybridization
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
JP5976849B2 (ja) 2012-03-05 2016-08-24 シージーン アイエヌシー ＰＴＯ切断及び伸長アッセイによるターゲット核酸配列でのヌクレオチド変異検出｛ＤｅｔｅｃｔｉｏｎｏｆＮｕｃｌｅｏｔｉｄｅＶａｒｉａｔｉｏｎｏｎＴａｒｇｅｔＮｕｃｌｅｉｃＡｃｉｄＳｅｑｕｅｎｃｅｂｙＰＴＯＣｌｅａｖａｇｅａｎｄＥｘｔｅｎｓｉｏｎＡｓｓａｙ｝
EP2839031B1 (en) * 2012-04-19 2017-03-29 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent signaling oligonucleotide cleavage
WO2014193198A1 (ko) 2013-05-30 2014-12-04 케이맥(주) 바이오 물질의 실시간 정량 및 정성 분석 방법
WO2015147370A1 (en) * 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
WO2016093620A1 (en) 2014-12-09 2016-06-16 Seegene, Inc. Differentiation of signals for target nucleic acid sequences
CA2970966A1 (en) 2014-12-22 2016-06-30 Anapa Biotech A/S Dual quenching assay for multiplex detection of target nucleic acids
JP6685396B2 (ja) 2015-11-20 2020-04-22 シージーン アイエヌシー 標的分析物質に対するデータセットの補正方法
WO2017135756A1 (en) 2016-02-05 2017-08-10 Seegene, Inc. Method for reducing noise level of data set for a target analyte
KR101961642B1 (ko) * 2016-04-25 2019-03-25 (주)진매트릭스 절단된 상보적인 태그 절편을 이용한 표적 핵산 서열 검출 방법 및 그 조성물
KR102218776B1 (ko) 2016-06-10 2021-02-22 주식회사 씨젠 태깅 올리고뉴클레오타이드의 제공 방법
KR102301305B1 (ko) 2016-06-30 2021-09-13 주식회사 씨젠 핵산 증폭용 튜브 및 핵산 증폭용 반응 용기
KR102468174B1 (ko) * 2016-09-15 2022-11-17 에프. 호프만-라 로슈 아게 멀티플렉스 pcr 수행 방법
EP3559275A1 (de) * 2016-12-23 2019-10-30 Albert-Ludwigs-Universität Freiburg Zweiteilige mediatorsonde
WO2018124665A1 (en) 2016-12-29 2018-07-05 Seegene, Inc. . Method for reducing primer dimer formation and increasing amplification efficiency
KR102408564B1 (ko) 2017-03-28 2022-06-14 주식회사 씨젠 타겟 핵산 서열의 존재를 결정하기 위한 분석 시그널
CN108823287B (zh) * 2017-04-28 2019-04-12 厦门大学 一种检测靶核酸序列的方法
KR102380264B1 (ko) 2017-08-31 2022-03-29 주식회사 씨젠 다이머-형성 프라이머 쌍을 이용한 구성요소의 성능 평가
WO2019066461A2 (en) 2017-09-29 2019-04-04 Seegene, Inc. DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY CLEAVAGE ANALYSIS AND EXTENSION OF PTO
US20190112636A1 (en) * 2017-10-16 2019-04-18 ChromaCode, Inc. Methods and compositions for nucleic acid detection
WO2019135567A1 (en) 2018-01-05 2019-07-11 Seegene, Inc. . Method for determining the presence or absence of m. tuberculosis, m. bovis and m. bovis bcg in a sample
US20210040542A1 (en) 2018-04-20 2021-02-11 Seegene, Inc. Method And Apparatus For Detecting A Plurality Of Target Nucleic Acid Sequences In Sample
CN114250275B (zh) * 2020-09-22 2023-04-28 武汉艾米森生命科技有限公司 一种荧光定量pcr反应系统、pcr反应试剂盒及核酸定量检测方法
CN114622000B (zh) * 2020-12-14 2024-05-07 厦门大学 一种高特异性的检测靶核酸序列的方法
CN112592964A (zh) * 2020-12-17 2021-04-02 厦门大学 用于进行核酸多重检测的方法
WO2022192758A1 (en) * 2021-03-11 2022-09-15 BioSpyder Technologies, Inc. Tempo-span
KR20240058112A (ko) * 2021-09-17 2024-05-03 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출
WO2023054848A1 (ko) 2021-09-30 2023-04-06 주식회사 씨젠 분자진단 시스템의 샘플 처리 및 분석 방법
WO2023068823A1 (ko) 2021-10-21 2023-04-27 주식회사 씨젠 시료 내 표적 분석물질에 대한 양음성 판독 장치 및 방법
CN114032337A (zh) * 2021-12-14 2022-02-11 广州市金圻睿生物科技有限责任公司 一种呼吸道病原体检测试剂盒及其制备方法与应用
CN114134219A (zh) * 2021-12-14 2022-03-04 广州市金圻睿生物科技有限责任公司 一种多重核酸检测系统及其制备方法与应用
KR20240028084A (ko) 2022-08-24 2024-03-05 주식회사 멀티렉스 표지자가 결합된 태그 프로브 및 이의 용도
WO2024106890A1 (ko) * 2022-11-16 2024-05-23 주식회사 씨젠 퀀칭-pto 절단 및 연장(quenching-pto cleavage and extension; q-ptoce) 어세이에 의한 타겟 핵산의 검출

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5541311A (en) 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
DK0966542T3 (da) 1996-11-29 2009-03-23 Third Wave Tech Inc FEN-1 endonukleaser, blandinger og spaltningsmethoder
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6322980B1 (en) 1999-04-30 2001-11-27 Aclara Biosciences, Inc. Single nucleotide detection using degradation of a fluorescent sequence
JP4724380B2 (ja) * 1999-04-20 2011-07-13 独立行政法人産業技術総合研究所 核酸の測定方法に用いる核酸プローブおよびデータを解析する方法
US7381532B2 (en) * 1999-10-29 2008-06-03 Stratagene California Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US6893819B1 (en) 2000-11-21 2005-05-17 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US7585632B2 (en) 1999-10-29 2009-09-08 Hologic, Inc. Compositions and methods for the detection of a nucleic acid using a cleavage reaction
WO2001090417A2 (en) * 2000-05-19 2001-11-29 Eragen Biosciences, Inc. Materials and methods for detection of nucleic acids
US7678541B2 (en) * 2000-11-21 2010-03-16 Hologic, Inc. Methods and compositions for the detection of a nucleic acid using a non-invasive cleavage reaction
US7309573B2 (en) 2000-11-21 2007-12-18 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
WO2003033741A1 (en) 2001-10-16 2003-04-24 Aclara Biosciences, Inc. Universal e-tag primer and probe compositions and methods
AU2003217379A1 (en) * 2002-02-15 2003-09-09 Somalogic, Inc. Methods and reagents for detecting target binding by nucleic acid ligands
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US8206904B2 (en) 2002-12-18 2012-06-26 Third Wave Technologies, Inc. Detection of nucleic acids
US20070092880A1 (en) 2003-07-16 2007-04-26 Crothers Donald M Invasive cleavage reaction with electrochemical readout
WO2005047470A2 (en) * 2003-11-07 2005-05-26 U.S. Genomics, Inc. Intercalator fret donors or acceptors
WO2005059548A1 (ja) 2003-12-19 2005-06-30 Kankyo Engineering Co., Ltd. 核酸測定用新規混合物、及びそれを用いる核酸の新規測定方法並びにそれらに用いる核酸プローブ
CA2497324A1 (en) 2004-02-17 2005-08-17 Affymetrix, Inc. Methods for fragmenting and labelling dna
WO2006005081A2 (en) * 2004-06-30 2006-01-12 Applera Corporation Compositions and methods for identifying nucleotides in polynucleotide sequences
WO2006004949A1 (en) 2004-06-30 2006-01-12 Applera Corporation Compositions and methods for detecting and quantitating polynucleotide sequences
WO2006128010A2 (en) 2005-05-26 2006-11-30 The Trustees Of Boston University Quantification of nucleic acids and proteins using oligonucleotide mass tags
WO2007011946A2 (en) * 2005-07-15 2007-01-25 Applera Corporation Detection of nucleic acid amplification
EP2118310B1 (en) * 2006-12-29 2013-03-06 Applied Biosystems, LLC Systems and methods for detecting nucleic acids
AU2007339793A1 (en) * 2006-12-29 2008-07-10 Applied Biosystems, Llc Systems and methods for detecting nucleic acids
FI20075124A0 (fi) 2007-02-21 2007-02-21 Valtion Teknillinen Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi
KR20090067334A (ko) 2007-12-21 2009-06-25 주식회사 씨젠 핵산 중합효소의 뉴클레아제 활성을 이용한 타깃핵산분자의 검출방법
US20090253142A1 (en) 2008-03-15 2009-10-08 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules during amplification reactions
CN102171366B (zh) 2008-07-31 2014-11-26 奥西泰克有限公司 多重扩增与检测
CN102186997B (zh) * 2008-08-15 2014-12-24 卡斯凯德生物体系股份有限公司 检测核酸
GB0818355D0 (en) * 2008-10-08 2008-11-12 Smart Patents Ltd Universal beverage container marker
CN102216472A (zh) * 2008-11-13 2011-10-12 里博克斯艾克斯有限公司 Rna检测方法
EP2256215A1 (en) 2009-04-30 2010-12-01 Steffen Mergemeier Assay system using a nuclease activity of a nucleic acid polymerase
EP2248915B1 (en) * 2009-05-05 2013-09-18 Qiagen GmbH Detection of multiple nucleic acid sequences in a reaction cartridge
WO2011027966A2 (en) 2009-09-03 2011-03-10 Seegene, Inc. Td probe and its uses
WO2011078441A1 (en) * 2009-12-21 2011-06-30 Seegene, Inc. Tsg primer target detection
EP2569449A4 (en) * 2010-05-14 2013-12-04 Life Technologies Corp IDENTIFICATION OF NUCLEIC ACIDS
MX340258B (es) 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
US11078525B2 (en) * 2011-03-29 2021-08-03 Seegene, Inc. Detection of target nucleic acid sequence by PTO cleavage and extension-dependent cleavage
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출

Also Published As

Publication number Publication date
BR112014018940A2 (pt) 2017-06-20
IL233773B (en) 2018-12-31
MX2014009403A (es) 2015-07-06
SG11201404349YA (en) 2014-08-28
US10731203B2 (en) 2020-08-04
EP2809797B1 (en) 2018-11-28
NZ627919A (en) 2016-11-25
IL233773A0 (en) 2014-09-30
CN104145029A (zh) 2014-11-12
KR20130101952A (ko) 2013-09-16
EP2809797A4 (en) 2015-09-09
NO20140948A1 (no) 2014-10-30
ZA201405463B (en) 2015-12-23
RU2014133695A (ru) 2016-03-27
AU2016204887B2 (en) 2018-01-25
EP2809797A1 (en) 2014-12-10
CA2862488A1 (en) 2013-08-08
BR112014018940B1 (pt) 2021-03-23
CA2862488C (en) 2018-11-20
CN104145029B (zh) 2016-06-01
AU2016204887A1 (en) 2016-07-28
AU2012368436A1 (en) 2014-08-21
KR101832867B1 (ko) 2018-03-02
US9868980B2 (en) 2018-01-16
KR20140112060A (ko) 2014-09-22
CN106011234B (zh) 2019-11-12
RU2608501C2 (ru) 2017-01-18
JP6005180B2 (ja) 2016-10-12
AU2012368436B2 (en) 2016-05-12
JP2015505470A (ja) 2015-02-23
US20150072887A1 (en) 2015-03-12
US20180127812A1 (en) 2018-05-10
WO2013115442A1 (en) 2013-08-08
MX361903B (es) 2018-12-18
CN106011234A (zh) 2016-10-12

Similar Documents

Publication Publication Date Title
BR112014018940A8 (pt) Método e kit para detecção de uma sequência de ácido nucleico alvo
BR112012026221A2 (pt) métodos e kit para detectar sequência de ácido nucléico-alvo de um dna ou uma mistura de ácidos nucleicos através de um ensaio de ptoce
WO2013184754A3 (en) Spatial sequencing of nucleic acids using dna origami probes
WO2012104340A3 (en) Markers of melanoma and uses thereof
WO2015123430A3 (en) Single molecule electronic multiplex snp assay and pcr analysis
IN2015DN01300A (pt)
EA201171131A1 (ru) Усовершенствованное определение экспрессии генов
CA2840149C (en) Methods and nucleic acids for determining the prognosis of a cancer subject
BR112016029548A2 (pt) método de sequenciamento de um ácido nucleico e sistema de sonda biológico multicomponente
WO2014182528A3 (en) Multiplex labeling of molecules by sequential hybridization barcoding
BR112014005205A2 (pt) métodos para etiquetar bibliotecas codificadas com dna
MY157531A (en) Td probe and its uses
GB201317477D0 (en) Methods, compositions and kits for determing the presence/absnece of a variant nucleic acid sequence
EP2756101A4 (en) SAMPLE: ANTIPROBE COMPOSITIONS FOR DETECTION OF HIGH-SPECIFIC DNA OR RNA
WO2015083001A3 (en) Nucleic acid probe and method of detecting genomic fragments
BR112012030813A2 (pt) "métodos e kits para detecção in situ de sequências de nucleotídeo"
HK1219760A1 (zh) 用於分析單鏈核酸序列的試劑盒
MX2015008494A (es) Deteccion de secuencia de acidos nucleicos objetivo por ensayo de escision de pto y no hibridacion dependiente de la extension.
EP2823061A4 (en) DETECTION OF NUCLEOTIDE VARIATION OF NUCLEIC ACID SEQUENCE BY PTO CLEAVAGE AND EXTENSION TEST
JP2014500729A5 (pt)
BR112015008621A2 (pt) Métodos e kit de diagnóstico e/ou prognóstico in vitro microconjunto de ácidos nucleicos.
BRPI1006411A8 (pt) Seleção genômica e sequenciamento utilizando microcarreadores codificados, processo para determinar a sequência de uma molécula de ácido nucleico alvo, e, dispositivo para determinar a sequência de uma amostra de ácido nucleico
EP2839031A4 (en) RECOGNITION OF TARGET NUCLEIC ACID SEQUENCE BY PTO CREATION AND EXPANSION-RELATED SIGNALING OF OLIGONUCLEOTIDE CLIP
MY184632A (en) A method of identifying parentage in freshwater prawn macrobrachium rosenbergii
WO2013102108A3 (en) Nucleic acid hybridization probes

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 03/07/2012, OBSERVADAS AS CONDICOES LEGAIS.