MX361903B - Deteccion de secuencia de acidos nucleicos objetivo mediante ensayo de escision de pto e hibridacion de oligonucleotidos de señalizacion dependiente de la extension. - Google Patents

Deteccion de secuencia de acidos nucleicos objetivo mediante ensayo de escision de pto e hibridacion de oligonucleotidos de señalizacion dependiente de la extension.

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Publication number
MX361903B
MX361903B MX2014009403A MX2014009403A MX361903B MX 361903 B MX361903 B MX 361903B MX 2014009403 A MX2014009403 A MX 2014009403A MX 2014009403 A MX2014009403 A MX 2014009403A MX 361903 B MX361903 B MX 361903B
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MX
Mexico
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MX2014009403A
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English (en)
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MX2014009403A (es
Inventor
Young Jo Lee
Joug Yoon Chun
Original Assignee
Seegene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seegene Inc filed Critical Seegene Inc
Priority claimed from PCT/KR2012/005284 external-priority patent/WO2013005974A2/ko
Publication of MX2014009403A publication Critical patent/MX2014009403A/es
Publication of MX361903B publication Critical patent/MX361903B/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

La presente invención se refiere a la detección de una secuencia de ácidos nucleicos objetivo mediante un ensayo de PCE-SH (Escisión de PTO e Hibridación de Oligonucleótidos de Señalización Dependiente de la Extensión). La presente invención no utiliza sondas que se hibridan con secuencias de ácidos nucleicos objetivo para proporcionar señales objetivo. De manera interesante, la presente invención utiliza sondas (oligonucleótidos de señalización) que se hibridan con la cadena extendida formada de una manera dependiente del objetivo en la que la cadena extendida se sintetiza utilizando el CTO seleccionado artificialmente como plantillas.
MX2014009403A 2012-02-02 2012-07-03 Deteccion de secuencia de acidos nucleicos objetivo mediante ensayo de escision de pto e hibridacion de oligonucleotidos de señalizacion dependiente de la extension. MX361903B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20120010681 2012-02-02
KR1020120028429A KR20130101952A (ko) 2012-02-02 2012-03-20 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
PCT/KR2012/005284 WO2013005974A2 (ko) 2011-07-05 2012-07-03 친환경 습도지시카드

Publications (2)

Publication Number Publication Date
MX2014009403A MX2014009403A (es) 2015-07-06
MX361903B true MX361903B (es) 2018-12-18

Family

ID=49451920

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014009403A MX361903B (es) 2012-02-02 2012-07-03 Deteccion de secuencia de acidos nucleicos objetivo mediante ensayo de escision de pto e hibridacion de oligonucleotidos de señalizacion dependiente de la extension.

Country Status (15)

Country Link
US (2) US9868980B2 (es)
EP (1) EP2809797B1 (es)
JP (1) JP6005180B2 (es)
KR (2) KR20130101952A (es)
CN (2) CN106011234B (es)
AU (2) AU2012368436B2 (es)
BR (1) BR112014018940B1 (es)
CA (1) CA2862488C (es)
IL (1) IL233773B (es)
MX (1) MX361903B (es)
NO (1) NO20140948A1 (es)
RU (1) RU2608501C2 (es)
SG (1) SG11201404349YA (es)
WO (1) WO2013115442A1 (es)
ZA (1) ZA201405463B (es)

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Also Published As

Publication number Publication date
JP2015505470A (ja) 2015-02-23
US20180127812A1 (en) 2018-05-10
WO2013115442A1 (en) 2013-08-08
US10731203B2 (en) 2020-08-04
US9868980B2 (en) 2018-01-16
KR20130101952A (ko) 2013-09-16
CA2862488C (en) 2018-11-20
IL233773B (en) 2018-12-31
CA2862488A1 (en) 2013-08-08
BR112014018940A8 (pt) 2017-07-11
AU2012368436B2 (en) 2016-05-12
CN106011234A (zh) 2016-10-12
CN104145029A (zh) 2014-11-12
JP6005180B2 (ja) 2016-10-12
RU2608501C2 (ru) 2017-01-18
NZ627919A (en) 2016-11-25
CN106011234B (zh) 2019-11-12
AU2016204887B2 (en) 2018-01-25
AU2016204887A1 (en) 2016-07-28
RU2014133695A (ru) 2016-03-27
IL233773A0 (en) 2014-09-30
EP2809797A4 (en) 2015-09-09
EP2809797B1 (en) 2018-11-28
KR20140112060A (ko) 2014-09-22
KR101832867B1 (ko) 2018-03-02
BR112014018940A2 (es) 2017-06-20
US20150072887A1 (en) 2015-03-12
AU2012368436A1 (en) 2014-08-21
EP2809797A1 (en) 2014-12-10
CN104145029B (zh) 2016-06-01
BR112014018940B1 (pt) 2021-03-23
ZA201405463B (en) 2015-12-23
NO20140948A1 (no) 2014-10-30
SG11201404349YA (en) 2014-08-28
MX2014009403A (es) 2015-07-06

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