HRP20150190T1 - Postupak za utvrđivanje tipa humanog papilomavirusa (hpv) - Google Patents
Postupak za utvrđivanje tipa humanog papilomavirusa (hpv) Download PDFInfo
- Publication number
- HRP20150190T1 HRP20150190T1 HRP20150190TT HRP20150190T HRP20150190T1 HR P20150190 T1 HRP20150190 T1 HR P20150190T1 HR P20150190T T HRP20150190T T HR P20150190TT HR P20150190 T HRP20150190 T HR P20150190T HR P20150190 T1 HRP20150190 T1 HR P20150190T1
- Authority
- HR
- Croatia
- Prior art keywords
- seq
- oligonucleotide sequence
- hpv
- nucleic acid
- pcr primers
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims 6
- 241000701806 Human papillomavirus Species 0.000 title 1
- 238000001514 detection method Methods 0.000 title 1
- 108091034117 Oligonucleotide Proteins 0.000 claims 12
- 150000007523 nucleic acids Chemical group 0.000 claims 8
- 238000006243 chemical reaction Methods 0.000 claims 6
- 230000003321 amplification Effects 0.000 claims 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 238000010791 quenching Methods 0.000 claims 4
- 230000000171 quenching effect Effects 0.000 claims 4
- 239000000523 sample Substances 0.000 claims 4
- 238000011144 upstream manufacturing Methods 0.000 claims 4
- 239000012472 biological sample Substances 0.000 claims 2
- 238000011002 quantification Methods 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/708—Specific hybridization probes for papilloma
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Claims (5)
1. Postupak za istovremeno utvrđivanje i/ili tipizaciju i/ili kvantifikaciju prisutnosti HPV 18, 52, 59, 39, 51, 56, 66, 16, 45, 58, 31, 33 i 35 u biološkom uzorku, naznačen time da postupak sadrži sljedeće četiri paralelne reakcije:
Reakcija 1-
a) umnažanje fragmenata nukleinskih kiselina od HPV 18, 52 i 59 u prisutnosti
(i) polimeraze nukleinske kiseline
(ii) nizvodne PCR početnice s oligonukleotidnom sekvencom od SEQ ID NO: 1,
(iii) uzvodne PCR početnice s oligonukleotidnom sekvencom od SEQ ID NO: 2,
(iv) probe s oligonukleotidnom sekvencom od SEQ ID NO: 7,8 i 9, označene s fluoroforom i molekulom za gašenje i
b) otkrivanje promjene fluorescencije njihove valne duljine koja određuje HPV tip 18, 52 i 59;
Reakcija 2-
a) umnažanje fragmenata nukleinskih kiselina od HPV 39,51,56 i 66 u prisutnosti
(i) polimeraze nukleinske kiseline
(ii) nizvodne PCR početnice s oligonukleotidnom sekvencom od SEQ ID NO: 1,
(iii) uzvodne PCR početnice s oligonukleotidnom sekvencom od SEQ ID NO: 3,
(iv) probe s oligonukleotidnom sekvencom od SEQ ID NO: 10, 11, 12 i 13, označene s fluoroforom i molekulom za gašenje i
b) otkrivanje promjene fluorescencije njihove valne duljine koja određuje HPV tip 39, 51, 56 i 66;
Reakcija 3-
a) umnažanje fragmenata nukleinskih kiselina od HPV 16,45 i 58 u prisutnosti
(i) polimeraze nukleinske kiseline
(ii) nizvodne PCR početnice s oligonukleotidnom sekvencom od SEQ ID NO: 1,
(iii) uzvodne PCR početnice s oligonukleotidnom sekvencom od SEQ ID NO: 4,
(iv) probe s oligonukleotidnom sekvencom od SEQ ID NO: 14, 15 i 16, označene s fluoroforom i molekulom za gašenje i
b) otkrivanje promjene fluorescencije njihove valne duljine koja određuje HPV tip 16, 45 i 58;
Reakcija 4-
a) umnažanje fragmenata nukleinskih kiselina od HPV 31, 33, 35 i unutrašnje kontrole u prisutnosti
(i) polimeraze nukleinske kiseline
(ii) nizvodne PCR početnice s oligonukleotidnom sekvencom od SEQ ID NO: 1,
(iii) uzvodne PCR početnice s oligonukleotidnom sekvencom od SEQ ID NO: 5,
(iv) probe s oligonukleotidnom sekvencom od SEQ ID NO: 17, 18, 19 i 20, označene s fluoroforom i molekulom za gašenje i
(v) unutrašnje kontrole, te
b) otkrivanje promjene fluorescencije njihove valne duljine koja određuje HPV tip 31, 33, 35 i unutrašnju kontrolu.
2. Postupak prema zahtjevu 1, naznačen time da se navedene reakcije provode paralelno i u zasebnim bočicama.
3. Postupak prema zahtjevima 1-2, naznačen time da su četiri fluorofore FAM, VIC, NED i ROX.
4. Postupak prema zahtjevima 1-2, naznačen time da unutrašnja kontrola je HPV6.
5. Komplet za istovremeno utvrđivanje i/ili tipizaciju i/ili kvantifikaciju HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 i 66 u biološkom uzorku naznačen time da sadrži četiri kombinacije početnica-proba koje se koriste u skladu s patentnim zahtjevom 1.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NO20091733A NO330943B1 (no) | 2009-04-30 | 2009-04-30 | En metode for detektering og/eller typebestemmelse og/eller kvantifisering av human papillomavirus (HPV) type, primere og prober derav samt diagnostisk sett og anvendelse derav. |
| PCT/NO2010/000147 WO2010126375A1 (en) | 2009-04-30 | 2010-04-22 | A method for detection of human papillomavirus (hpv) type |
| EP10769997.7A EP2425017B1 (en) | 2009-04-30 | 2010-04-22 | A method for detection of human papillomavirus (hpv) type |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20150190T1 true HRP20150190T1 (hr) | 2015-06-05 |
Family
ID=43032356
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20150190TT HRP20150190T1 (hr) | 2009-04-30 | 2015-02-18 | Postupak za utvrđivanje tipa humanog papilomavirusa (hpv) |
Country Status (23)
| Country | Link |
|---|---|
| US (1) | US8895247B2 (hr) |
| EP (1) | EP2425017B1 (hr) |
| CN (1) | CN102459631B (hr) |
| AU (1) | AU2010242168B2 (hr) |
| BR (1) | BRPI1007637A2 (hr) |
| CA (1) | CA2760274A1 (hr) |
| CY (1) | CY1116083T1 (hr) |
| DK (1) | DK2425017T3 (hr) |
| EA (1) | EA201171239A1 (hr) |
| ES (1) | ES2534644T3 (hr) |
| HR (1) | HRP20150190T1 (hr) |
| IL (1) | IL216035A0 (hr) |
| MX (1) | MX2011011521A (hr) |
| MY (1) | MY160754A (hr) |
| NO (1) | NO330943B1 (hr) |
| NZ (1) | NZ596252A (hr) |
| PL (1) | PL2425017T3 (hr) |
| PT (1) | PT2425017E (hr) |
| SA (1) | SA110310331B1 (hr) |
| SG (1) | SG175825A1 (hr) |
| SI (1) | SI2425017T1 (hr) |
| WO (1) | WO2010126375A1 (hr) |
| ZA (1) | ZA201108225B (hr) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NO330943B1 (no) | 2009-04-30 | 2011-08-22 | Unilabs Telelabs As | En metode for detektering og/eller typebestemmelse og/eller kvantifisering av human papillomavirus (HPV) type, primere og prober derav samt diagnostisk sett og anvendelse derav. |
| CN102994647B (zh) * | 2012-06-21 | 2014-11-19 | 宁波海尔施基因科技有限公司 | 一种人乳头瘤病毒(hpv)的分型定量检测试剂盒 |
| RU2520710C1 (ru) * | 2012-12-10 | 2014-06-27 | Государственное бюджетное образовательное учреждение высшего профессионального образования "Челябинская государственная медицинская академия" Министерства здравоохранения и социального развития Российской Федерации (ГБОУ ВПО ЧелГМА Минздравсоцразвития России) | Способ прогнозирования персистенции онкогенных типов вируса папилломы человека в цервикальном эпителии |
| CN104818342B (zh) * | 2015-03-23 | 2017-09-22 | 厦门艾德生物医药科技有限公司 | 用于19种高危型人乳头瘤病毒(hpv)的检测试剂盒、检测体系及方法 |
| AU2016288666A1 (en) * | 2015-06-30 | 2018-01-04 | Psomagen, Inc. | Method and system for diagnostic testing |
| CN109554506A (zh) * | 2016-01-15 | 2019-04-02 | 艾康生物技术(杭州)有限公司 | 一种用于检测病原体的引物和探针的设计方法 |
| US11441167B2 (en) * | 2019-11-20 | 2022-09-13 | Roche Molecular Systems, Inc. | Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3853678T2 (de) * | 1987-02-26 | 1995-08-31 | Univ Sydney | Verfahren zum nachweis des karzinogenen menschlichen papillomavirus. |
| US5484699A (en) * | 1990-09-28 | 1996-01-16 | Abbott Laboratories | Nucleotide sequences useful as type specific probes, PCR primers and LCR probes for the amplification and detection of human papilloma virus, and related kits and methods |
| US5821087A (en) * | 1995-03-30 | 1998-10-13 | Merck & Co., Inc. | Production of recombinant human papillomavirus type II protein utilizing papillomavirus 6/11 hybrid DNA |
| WO1999014377A2 (en) | 1997-09-16 | 1999-03-25 | Innogenetics N.V. | Detection and identification of human papillomavirus by pcr and type-specific reverse hybridization |
| DE19903056B4 (de) * | 1999-01-26 | 2007-07-19 | Medeea Forschungs-Gmbh | HPV-Assay |
| GB0500996D0 (en) * | 2005-01-18 | 2005-02-23 | Delfts Diagnostic Labaratory B | Detection method and materials therefor |
| US7553955B2 (en) * | 2005-09-01 | 2009-06-30 | The Trustees Of The University Of Pennsylvania | Molecular beacons, methods and kits for detecting DNA damage response |
| WO2007115582A1 (en) | 2006-04-11 | 2007-10-18 | Bio-Rad Pasteur | Hpv detection and quantification by real-time multiplex amplification |
| WO2007130519A2 (en) | 2006-05-02 | 2007-11-15 | Government Of The Usa, As Represented By The Secretary, Department Of Health And Human Services | Viral nucleic acid microarray and method of use |
| GB0702557D0 (en) * | 2007-02-09 | 2007-03-21 | Health Prot Agency | Detection of human papillomavirus |
| WO2009011472A1 (en) | 2007-07-19 | 2009-01-22 | Hyunil Choi | Primers and proves for detection of high risk group geno-type human papillomavirus dna, a qualitative assay method of the same dna using them and a qualitative assay kit of the same dna |
| NO330943B1 (no) | 2009-04-30 | 2011-08-22 | Unilabs Telelabs As | En metode for detektering og/eller typebestemmelse og/eller kvantifisering av human papillomavirus (HPV) type, primere og prober derav samt diagnostisk sett og anvendelse derav. |
-
2009
- 2009-04-30 NO NO20091733A patent/NO330943B1/no not_active IP Right Cessation
-
2010
- 2010-04-22 SG SG2011079480A patent/SG175825A1/en unknown
- 2010-04-22 MX MX2011011521A patent/MX2011011521A/es active IP Right Grant
- 2010-04-22 MY MYPI2011005207A patent/MY160754A/en unknown
- 2010-04-22 EA EA201171239A patent/EA201171239A1/ru unknown
- 2010-04-22 US US13/266,943 patent/US8895247B2/en not_active Expired - Fee Related
- 2010-04-22 CN CN201080024379.XA patent/CN102459631B/zh not_active Expired - Fee Related
- 2010-04-22 BR BRPI1007637A patent/BRPI1007637A2/pt not_active IP Right Cessation
- 2010-04-22 WO PCT/NO2010/000147 patent/WO2010126375A1/en active Application Filing
- 2010-04-22 CA CA2760274A patent/CA2760274A1/en active Pending
- 2010-04-22 ES ES10769997.7T patent/ES2534644T3/es active Active
- 2010-04-22 DK DK10769997T patent/DK2425017T3/en active
- 2010-04-22 EP EP10769997.7A patent/EP2425017B1/en active Active
- 2010-04-22 SI SI201030881T patent/SI2425017T1/sl unknown
- 2010-04-22 PT PT107699977T patent/PT2425017E/pt unknown
- 2010-04-22 NZ NZ596252A patent/NZ596252A/xx not_active IP Right Cessation
- 2010-04-22 AU AU2010242168A patent/AU2010242168B2/en not_active Ceased
- 2010-04-22 PL PL10769997T patent/PL2425017T3/pl unknown
- 2010-04-28 SA SA110310331A patent/SA110310331B1/ar unknown
-
2011
- 2011-10-30 IL IL216035A patent/IL216035A0/en unknown
- 2011-11-09 ZA ZA2011/08225A patent/ZA201108225B/en unknown
-
2015
- 2015-02-18 HR HRP20150190TT patent/HRP20150190T1/hr unknown
- 2015-02-19 CY CY20151100171T patent/CY1116083T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| MX2011011521A (es) | 2012-04-19 |
| NZ596252A (en) | 2013-06-28 |
| WO2010126375A1 (en) | 2010-11-04 |
| CN102459631A (zh) | 2012-05-16 |
| EP2425017B1 (en) | 2014-11-19 |
| BRPI1007637A2 (pt) | 2016-02-23 |
| NO20091733L (no) | 2010-11-01 |
| US20120100528A1 (en) | 2012-04-26 |
| CY1116083T1 (el) | 2017-02-08 |
| IL216035A0 (en) | 2012-01-31 |
| US8895247B2 (en) | 2014-11-25 |
| ES2534644T3 (es) | 2015-04-27 |
| SA110310331B1 (ar) | 2014-08-18 |
| EA201171239A1 (ru) | 2012-05-30 |
| AU2010242168B2 (en) | 2014-11-20 |
| PL2425017T3 (pl) | 2015-07-31 |
| SI2425017T1 (sl) | 2015-05-29 |
| EP2425017A1 (en) | 2012-03-07 |
| MY160754A (en) | 2017-03-15 |
| ZA201108225B (en) | 2013-01-30 |
| AU2010242168A1 (en) | 2011-12-01 |
| EP2425017A4 (en) | 2012-12-26 |
| SG175825A1 (en) | 2011-12-29 |
| PT2425017E (pt) | 2015-02-27 |
| DK2425017T3 (en) | 2015-03-02 |
| CA2760274A1 (en) | 2010-11-04 |
| NO330943B1 (no) | 2011-08-22 |
| CN102459631B (zh) | 2014-07-02 |
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