CY1116130T1 - Μεθοδοι δοκιμασιας για mdv-1 - Google Patents
Μεθοδοι δοκιμασιας για mdv-1Info
- Publication number
- CY1116130T1 CY1116130T1 CY20151100277T CY151100277T CY1116130T1 CY 1116130 T1 CY1116130 T1 CY 1116130T1 CY 20151100277 T CY20151100277 T CY 20151100277T CY 151100277 T CY151100277 T CY 151100277T CY 1116130 T1 CY1116130 T1 CY 1116130T1
- Authority
- CY
- Cyprus
- Prior art keywords
- mdv
- nucleic acid
- sample
- probe
- infectious
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
- A61P31/22—Antivirals for DNA viruses for herpes viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Abstract
Μέθοδος για την ποσοτικοποίηση ενός εμβολίου στελέχους και/ή ενός μολυσματικού στελέχους του Ιού της Νόσου Wlarek Ορότυπος-1 (MDV-1) σε ένα δείγμα από ένα πτηνό, που περιλαμβάνει τα βήματα : (i) εξασφάλιση ενός βιολογικού δείγματος από το πτηνό και προαιρετικά απομόνωση του νουκλεϊνικού οξέος από το βιολογικό δείγμα, (ii) υποβολή του βιολογικού δείγματος του (i) σε PCR πραγματικού χρόνου qPCR που περιλαμβάνει: (a) ενίσχυση μιας περιοχής του pp3Β γονιδίου μέσα στο δείγμα του νουκλεϊνικού οξέος του δείγματος (i), όπου η αναφερθείσα περιοχή περιέχει μία διαφορά σε ένα σταθερό πολυμορφισμό ενός νουκλεοτιδίου (SNP) μεταξύ του εμβολίου και των μολυσματικών στελεχών του MDV-1, και (b) επαφή του επεκταμένου νουκλεϊνικού οξέος του (a) με ένα ανιχνεύσιμο ανιχνευτή νουκλεϊνικού οξέος ειδικού για το SNP του εμβολίου στελέχους του MDV-1 και/ή ένα ανιχνεύσιμο ανιχνευτή νουκλεϊνικού οξέος ειδικού για το SNP του μολυσματικού στελέχους MDV-1, και (iii) μέτρηση των αλλαγών στο σήμα του ανιχνευτού που παράγεται από τον ανιχνευτή του (ii). Μέθοδοι επίσης εξασφαλίζονται για την απόλυτη ποσοτικοποίηση του εμβολίου και των μολυσματικών στελεχών.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0914826.3A GB0914826D0 (en) | 2009-08-25 | 2009-08-25 | Assay methods |
EP10752901.8A EP2470676B1 (en) | 2009-08-25 | 2010-08-25 | Assay methods for mdv-1 |
Publications (1)
Publication Number | Publication Date |
---|---|
CY1116130T1 true CY1116130T1 (el) | 2017-02-08 |
Family
ID=41171882
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CY20151100277T CY1116130T1 (el) | 2009-08-25 | 2015-03-20 | Μεθοδοι δοκιμασιας για mdv-1 |
Country Status (17)
Country | Link |
---|---|
US (3) | US8865409B2 (el) |
EP (1) | EP2470676B1 (el) |
JP (2) | JP6025562B2 (el) |
CN (1) | CN102686748B (el) |
AU (1) | AU2010288269C1 (el) |
BR (1) | BR112012004102B1 (el) |
CA (1) | CA2771962C (el) |
CY (1) | CY1116130T1 (el) |
DK (1) | DK2470676T3 (el) |
ES (1) | ES2538189T3 (el) |
GB (1) | GB0914826D0 (el) |
HR (1) | HRP20150308T1 (el) |
MX (1) | MX2012002411A (el) |
PL (1) | PL2470676T3 (el) |
PT (1) | PT2470676E (el) |
SI (1) | SI2470676T1 (el) |
WO (1) | WO2011023950A1 (el) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0914826D0 (en) | 2009-08-25 | 2009-09-30 | Wyeth Corp | Assay methods |
WO2013192100A1 (en) * | 2012-06-18 | 2013-12-27 | The United States Of America, As Represented By The Secretary, Department Of Health & Human Services | Methods and compositions for detecting jc virus |
EP2845904A1 (en) * | 2013-09-06 | 2015-03-11 | Ceva Sante Animale | Recombinant Marek's disease viruses and uses thereof |
EP2889381A1 (en) * | 2013-12-27 | 2015-07-01 | Universite De Liege | Detection method for species specific identification of nucleic acid of interest |
CN105886662B (zh) * | 2016-01-26 | 2020-03-24 | 金宇保灵生物药品有限公司 | 快速选育猪瘟兔化弱毒株(c株)st敏感细胞系的方法及专用引物 |
CN108034767A (zh) * | 2018-01-03 | 2018-05-15 | 山东农业大学 | 鉴别血清i型马立克病毒基因缺失疫苗、传代致弱疫苗与野毒的引物、探针及试剂盒 |
WO2020098810A1 (en) | 2018-11-17 | 2020-05-22 | Beijing Bytedance Network Technology Co., Ltd. | Merge with motion vector difference in video processing |
CN109355434B (zh) * | 2018-11-26 | 2022-04-15 | 中国农业科学院哈尔滨兽医研究所(中国动物卫生与流行病学中心哈尔滨分中心) | 定量检测鸡马立克氏病毒Meq基因缺失疫苗株和野毒株的荧光定量PCR试剂盒及其应用 |
CN110699485B (zh) * | 2019-08-16 | 2023-03-28 | 广州千寻生物技术有限公司 | 一种快速检测马立克氏病病毒的rpa引物对、探针、试剂盒及检测方法 |
CN111733297A (zh) * | 2020-08-10 | 2020-10-02 | 中国兽医药品监察所 | 一种马立克氏病病毒血清3型荧光定量pcr试剂盒及其应用 |
CN113025734A (zh) * | 2021-03-19 | 2021-06-25 | 广东省农业科学院动物卫生研究所 | 鉴别布鲁氏菌疫苗株a19与野毒株的引物、探针及应用 |
CN114317827B (zh) * | 2022-01-07 | 2023-03-24 | 河南省农业科学院 | 一种鉴别不同血清型马立克病病毒感染与免疫的多重pcr引物、方法及应用 |
CN114540312B (zh) * | 2022-03-16 | 2023-03-24 | 河南省农业科学院 | 一种血清1型马立克病病毒pp38单克隆抗体的制备方法及应用 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5827738A (en) * | 1995-10-27 | 1998-10-27 | Board Of Trustees Operating Michigan State University | Sustainable chick cell line infected with Marek's disease virus |
US5702659A (en) | 1995-11-30 | 1997-12-30 | Corning Incorporated | Honeycomb extrusion die and methods |
GB0914826D0 (en) | 2009-08-25 | 2009-09-30 | Wyeth Corp | Assay methods |
CN101824488A (zh) * | 2010-04-09 | 2010-09-08 | 山东农业大学 | 鸡多重病毒核酸探针斑点杂交检测试剂盒 |
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2009
- 2009-08-25 GB GBGB0914826.3A patent/GB0914826D0/en not_active Ceased
-
2010
- 2010-08-25 CA CA2771962A patent/CA2771962C/en active Active
- 2010-08-25 CN CN201080043079.6A patent/CN102686748B/zh active Active
- 2010-08-25 DK DK10752901.8T patent/DK2470676T3/en active
- 2010-08-25 BR BR112012004102-9A patent/BR112012004102B1/pt active IP Right Grant
- 2010-08-25 ES ES10752901.8T patent/ES2538189T3/es active Active
- 2010-08-25 PT PT10752901T patent/PT2470676E/pt unknown
- 2010-08-25 AU AU2010288269A patent/AU2010288269C1/en active Active
- 2010-08-25 WO PCT/GB2010/001602 patent/WO2011023950A1/en active Application Filing
- 2010-08-25 SI SI201030866T patent/SI2470676T1/sl unknown
- 2010-08-25 JP JP2012526114A patent/JP6025562B2/ja active Active
- 2010-08-25 MX MX2012002411A patent/MX2012002411A/es active IP Right Grant
- 2010-08-25 PL PL10752901T patent/PL2470676T3/pl unknown
- 2010-08-25 US US13/391,563 patent/US8865409B2/en active Active
- 2010-08-25 EP EP10752901.8A patent/EP2470676B1/en active Active
-
2014
- 2014-08-25 US US14/467,941 patent/US9416428B2/en active Active
- 2014-08-25 US US14/467,856 patent/US9416427B2/en active Active
-
2015
- 2015-03-18 HR HRP20150308TT patent/HRP20150308T1/hr unknown
- 2015-03-20 CY CY20151100277T patent/CY1116130T1/el unknown
- 2015-09-28 JP JP2015189321A patent/JP2016052307A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
EP2470676A1 (en) | 2012-07-04 |
CA2771962C (en) | 2015-09-29 |
AU2010288269C1 (en) | 2015-11-26 |
AU2010288269A1 (en) | 2012-03-08 |
US9416428B2 (en) | 2016-08-16 |
PT2470676E (pt) | 2015-03-30 |
US20150024382A1 (en) | 2015-01-22 |
JP2016052307A (ja) | 2016-04-14 |
EP2470676B1 (en) | 2015-01-28 |
BR112012004102B1 (pt) | 2021-03-30 |
CN102686748A (zh) | 2012-09-19 |
BR112012004102A2 (pt) | 2016-03-15 |
CA2771962A1 (en) | 2011-03-03 |
GB0914826D0 (en) | 2009-09-30 |
US9416427B2 (en) | 2016-08-16 |
PL2470676T3 (pl) | 2015-06-30 |
US20120208177A1 (en) | 2012-08-16 |
MX2012002411A (es) | 2012-04-10 |
JP2013502916A (ja) | 2013-01-31 |
JP6025562B2 (ja) | 2016-11-16 |
ES2538189T3 (es) | 2015-06-17 |
DK2470676T3 (en) | 2015-02-16 |
SI2470676T1 (sl) | 2015-03-31 |
AU2010288269B2 (en) | 2015-05-21 |
US8865409B2 (en) | 2014-10-21 |
HRP20150308T1 (hr) | 2015-04-24 |
WO2011023950A1 (en) | 2011-03-03 |
US20150024383A1 (en) | 2015-01-22 |
CN102686748B (zh) | 2015-09-09 |
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