ES2684746T3 - Prueba de cribado para detectar la presencia de virus VPH oncogénicos - Google Patents

Prueba de cribado para detectar la presencia de virus VPH oncogénicos Download PDF

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Publication number
ES2684746T3
ES2684746T3 ES15722601.0T ES15722601T ES2684746T3 ES 2684746 T3 ES2684746 T3 ES 2684746T3 ES 15722601 T ES15722601 T ES 15722601T ES 2684746 T3 ES2684746 T3 ES 2684746T3
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ES
Spain
Prior art keywords
hpv
oncogenic
pan
sample
detect
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES15722601.0T
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English (en)
Inventor
Lucjan WYRWICZ
Jacek PODOLSKI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centrum Onkologii Inst Im Marii Sklodowskiej Curie
Centrum Onkologii - Instytut Im Marii Sklodowskiej-Curie
Cervico Sp zoo
Original Assignee
Centrum Onkologii Inst Im Marii Sklodowskiej Curie
Centrum Onkologii - Instytut Im Marii Sklodowskiej-Curie
Cervico Sp zoo
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Publication date
Application filed by Centrum Onkologii Inst Im Marii Sklodowskiej Curie, Centrum Onkologii - Instytut Im Marii Sklodowskiej-Curie, Cervico Sp zoo filed Critical Centrum Onkologii Inst Im Marii Sklodowskiej Curie
Application granted granted Critical
Publication of ES2684746T3 publication Critical patent/ES2684746T3/es
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/708—Specific hybridization probes for papilloma
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Método para determinar si una muestra resulta positiva en virus del papiloma humano (VPH) oncogénico, comprendiendo el método: combinar la muestra con cebadores para la amplificación con reacción en cadena de la polimerasa (PCR) en tiempo real de una región objetivo de genomas de VPH oncogénicos, y una sonda marcada pan-VPH capaz de emitir una señal tras la amplificación de la región objetivo, proporcionar condiciones para la PCR en tiempo real, y medir una señal emitida desde la sonda marcada pan-VPH, donde la detección de la señal indica que la muestra resulta positiva en VPH oncogénico, caracterizado por que la sonda pan-VPH es una molécula de ácido nucleico bloqueado marcada (LNA) comprendiendo la secuencia ACTTTCGTTT (SEQ ID N.º 25) o el complemento inverso de esta.
ES15722601.0T 2014-04-11 2015-04-11 Prueba de cribado para detectar la presencia de virus VPH oncogénicos Active ES2684746T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PL40786414 2014-04-11
PL407864A PL235847B1 (pl) 2014-04-11 2014-04-11 Zestaw i sposób do wykrywania obecności onkogennych wirusów HPV
PCT/PL2015/050007 WO2015156693A1 (en) 2014-04-11 2015-04-11 Screening test for detecting the presence of oncogenic hpv viruses

Publications (1)

Publication Number Publication Date
ES2684746T3 true ES2684746T3 (es) 2018-10-04

Family

ID=53177861

Family Applications (1)

Application Number Title Priority Date Filing Date
ES15722601.0T Active ES2684746T3 (es) 2014-04-11 2015-04-11 Prueba de cribado para detectar la presencia de virus VPH oncogénicos

Country Status (10)

Country Link
US (1) US20170029908A1 (es)
EP (1) EP3129512B1 (es)
JP (1) JP2017513522A (es)
AU (1) AU2015244506A1 (es)
CA (1) CA2945477A1 (es)
ES (1) ES2684746T3 (es)
HU (1) HUE039321T2 (es)
PL (1) PL235847B1 (es)
TR (1) TR201809154T4 (es)
WO (1) WO2015156693A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3279337A1 (en) * 2016-08-04 2018-02-07 Servizo Galego de Saúde (SERGAS) Use of short probes between 8 and 9 nucleotides in multiplex assays
CN110699472A (zh) * 2019-11-21 2020-01-17 四川大学华西第二医院 一种用于鉴定hpv高危人群的标志物及其引物和检测方法
CN112195277B (zh) * 2020-10-29 2022-07-26 北京康美天鸿生物科技有限公司 基于实时荧光定量pcr检测人乳头瘤病毒的引物探针组以及试剂盒

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2629458B2 (fr) * 1987-07-31 1991-08-09 Ire Celltarg Sa Nouvelles sondes d'acides nucleiques specifiques de differents types de virus de papillome humain
BE1006179A3 (fr) * 1992-09-22 1994-05-31 Lambdatech S A Conjugue destine a la detection et/ou au dosage d'un compose biologique.
JP2009531433A (ja) * 2006-03-24 2009-09-03 ノバルティス アクチエンゲゼルシャフト ＨＰＶ感染処置のためのｄｓＲＮＡ組成物および方法
EP1997914A1 (en) 2007-06-01 2008-12-03 Università Degli Studi Di Milano - Bicocca Identification and quantification of oncogenic HPV nucleic acids by means of real-time PCR assays
JP2011518333A (ja) * 2008-04-17 2011-06-23 キアジェン ゲイサーズバーグ インコーポレイテッド 標的核酸の存在を判別するための合成プローブを用いた組成物、方法、およびキット
WO2011094514A1 (en) * 2010-01-29 2011-08-04 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
CA2828224A1 (en) * 2011-02-24 2012-08-30 Qiagen Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acids

Also Published As

Publication number Publication date
HUE039321T2 (hu) 2018-12-28
EP3129512A1 (en) 2017-02-15
EP3129512B1 (en) 2018-03-28
US20170029908A1 (en) 2017-02-02
WO2015156693A1 (en) 2015-10-15
PL235847B1 (pl) 2020-11-02
AU2015244506A1 (en) 2016-11-03
TR201809154T4 (tr) 2018-07-23
PL407864A1 (pl) 2015-10-12
JP2017513522A (ja) 2017-06-01
CA2945477A1 (en) 2015-10-15

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