BR112017006905A2 - oligonucleotídeo, conjunto de oligonucleotídeos, método para detecção de pelo um alvo de htlv, kit, polinucleotídeo, e, amplicon - Google Patents

oligonucleotídeo, conjunto de oligonucleotídeos, método para detecção de pelo um alvo de htlv, kit, polinucleotídeo, e, amplicon

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Publication number
BR112017006905A2
BR112017006905A2 BR112017006905A BR112017006905A BR112017006905A2 BR 112017006905 A2 BR112017006905 A2 BR 112017006905A2 BR 112017006905 A BR112017006905 A BR 112017006905A BR 112017006905 A BR112017006905 A BR 112017006905A BR 112017006905 A2 BR112017006905 A2 BR 112017006905A2
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BR
Brazil
Prior art keywords
htlv
oligonucleotide
amplicon
polynucleotide
kit
Prior art date
Application number
BR112017006905A
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English (en)
Other versions
BR112017006905B1 (pt
Inventor
Tadeu Covas Dimas
Cristiano Rocha Junior Maurício
Kashima Haddad Simone
Original Assignee
Fund Hemocentro De Ribeirao Preto
Univ Sao Paulo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Fund Hemocentro De Ribeirao Preto, Univ Sao Paulo filed Critical Fund Hemocentro De Ribeirao Preto
Publication of BR112017006905A2 publication Critical patent/BR112017006905A2/pt
Publication of BR112017006905B1 publication Critical patent/BR112017006905B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/112—Disease subtyping, staging or classification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

a presença do vírus linfotrópico de células t humanas (htlv) pode ser detectada e vírus pode ser tipado como tipo 1 ou 2 pelo método aqui descrito, que envolve a amplificação de sequências de dna do htlv por reação de polimerase em cadeia em tempo real. para isto, foram desenvolvidos iniciadores utilizados para amplificar uma região particular do genoma do htlv1 e 2. a presença do htlv-1 e/ou do htlv-2 em uma amostra é indicada pela formação de fluorescência liberada pelas sondas específicas para cada subtipo.
BR112017006905-9A 2014-10-06 2015-09-25 Conjunto de oligonucleotídeos, método para detectar e discriminar alvos htlv1/2 e kit para diagnóstico e discriminação da infecção pelo htlv1/2 BR112017006905B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
BR102014024905A BR102014024905A2 (pt) 2014-10-06 2014-10-06 oligonucleotídeos, conjunto de oligonucleotídeos, kit para diagnóstico e discriminação de infecção por htlv-1/2, polinucleotídeo adequado para uso como alvo de referência para o desenho de iniciadores e sondas para detecção e diferenciação de htlv-1 e htlv-2, amplicon, e, método para detecção de pelo menos um alvo de htlv
BR1020140249052 2014-10-06
PCT/BR2015/050161 WO2016054708A1 (pt) 2014-10-06 2015-09-25 Oligonucleotídeos, conjunto de oligonucleotídeos, kit para diagnóstico e discriminação de infecção por htlv-1/2, polinucleotídeo adequado para uso como alvo de referência para o desenho de iniciadores e sondas para detecção e diferenciação de htlv-1 e htlv- 2, amplicon, e, método para detecção de pelo menos um alvo de htlv

Publications (2)

Publication Number Publication Date
BR112017006905A2 true BR112017006905A2 (pt) 2018-03-27
BR112017006905B1 BR112017006905B1 (pt) 2024-04-30

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
BR102014024905A BR102014024905A2 (pt) 2014-10-06 2014-10-06 oligonucleotídeos, conjunto de oligonucleotídeos, kit para diagnóstico e discriminação de infecção por htlv-1/2, polinucleotídeo adequado para uso como alvo de referência para o desenho de iniciadores e sondas para detecção e diferenciação de htlv-1 e htlv-2, amplicon, e, método para detecção de pelo menos um alvo de htlv
BR112017006905-9A BR112017006905B1 (pt) 2014-10-06 2015-09-25 Conjunto de oligonucleotídeos, método para detectar e discriminar alvos htlv1/2 e kit para diagnóstico e discriminação da infecção pelo htlv1/2

Family Applications Before (1)

Application Number Title Priority Date Filing Date
BR102014024905A BR102014024905A2 (pt) 2014-10-06 2014-10-06 oligonucleotídeos, conjunto de oligonucleotídeos, kit para diagnóstico e discriminação de infecção por htlv-1/2, polinucleotídeo adequado para uso como alvo de referência para o desenho de iniciadores e sondas para detecção e diferenciação de htlv-1 e htlv-2, amplicon, e, método para detecção de pelo menos um alvo de htlv

Country Status (4)

Country Link
US (1) US20190055599A1 (pt)
EP (1) EP3214181B1 (pt)
BR (2) BR102014024905A2 (pt)
WO (1) WO2016054708A1 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108203738A (zh) * 2018-03-26 2018-06-26 郑州安图生物工程股份有限公司 一种用于检测人嗜t淋巴细胞病毒1型的试剂盒
CN108866156A (zh) * 2018-07-19 2018-11-23 北京世纪元亨动物防疫技术有限公司 用于poct荧光rt-pcr体系的单体系酶扩增组合物的制备方法及其应用
CN113136453A (zh) * 2020-01-19 2021-07-20 上海爱萨尔生物科技有限公司 特异性检测htlv-ii前病毒dna的引物及其应用
CN111926118A (zh) * 2020-08-21 2020-11-13 苏州育德扬生物技术有限公司 一种可同时检测艾滋病毒1、2型病毒以及人类t淋巴细胞白血病病毒1、2型的检测方法
CN116694822B (zh) * 2023-06-16 2024-01-30 中山市中心血站 实时荧光环介导恒温扩增检测人类嗜t淋巴细胞i型病毒的方法及试剂盒

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05504475A (ja) * 1989-11-30 1993-07-15 ファルマシア　バイオテック　インコーポレイテッド 細胞試料において特定の核酸配列を検出する方法
WO1993013225A1 (en) * 1991-12-23 1993-07-08 Chiron Corporation Htlv-1 probes for use in solution phase sandwich hybridization assays
US6406841B1 (en) * 1993-07-01 2002-06-18 Abbott Laboratories Methods for the detection of HTLV-II antibodies employing novel HTLV-II NRA envelope peptides
US6218522B1 (en) * 1996-03-19 2001-04-17 Shionogi & Co., Ltd. DNA molecule relating to suppression of gene expression and novel protein
USH1825H (en) * 1998-01-30 1999-12-07 Akzo Nobel N.V. Isothermal transcription based assay for the detection of HTLV I and HTLV II RNA
US7566532B1 (en) * 2004-07-02 2009-07-28 Quest Diagnostics Investments Incorporated Methods for detecting retroviruses
CN103898239B (zh) * 2014-03-10 2016-05-25 上海艾迪康医学检验所有限公司 一种同管检测htlv-i和htlv-ii前病毒的引物和方法

Also Published As

Publication number Publication date
BR112017006905B1 (pt) 2024-04-30
US20190055599A1 (en) 2019-02-21
EP3214181A1 (en) 2017-09-06
WO2016054708A1 (pt) 2016-04-14
EP3214181B1 (en) 2019-10-30
EP3214181A4 (en) 2018-07-04
BR102014024905A2 (pt) 2016-04-12

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B25D Requested change of name of applicant approved

Owner name: FUNDACAO HEMOCENTRO DE RIBEIRAO PRETO (BR/SP) ; UNIVERSIDADE DE SAO PAULO - USP (BR/SP)

B25L Entry of change of name and/or headquarter and transfer of application, patent and certificate of addition of invention: publication cancelled

Owner name: FUNDACAO HEMOCENTRO DE RIBEIRAO PRETO (BR/SP) ; UNIVERSIDADE DE SAO PAULO - USP (BR/SP)

Free format text: ANULADA A PUBLICACAO CODIGO 25.4 NA RPI NO 2773 DE 27/02/2024 POR TER SIDO INDEVIDA.

B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 25/09/2015, OBSERVADAS AS CONDICOES LEGAIS