BR112017022724A2 - planta de milho tolerante a herbicida dbn9858 e sequência de nucleotídeos e método para detectar a mesma - Google Patents

planta de milho tolerante a herbicida dbn9858 e sequência de nucleotídeos e método para detectar a mesma

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Publication number
BR112017022724A2
BR112017022724A2 BR112017022724-0A BR112017022724A BR112017022724A2 BR 112017022724 A2 BR112017022724 A2 BR 112017022724A2 BR 112017022724 A BR112017022724 A BR 112017022724A BR 112017022724 A2 BR112017022724 A2 BR 112017022724A2
Authority
BR
Brazil
Prior art keywords
dbn9858
detect
methods
nucleic acid
acid sequences
Prior art date
Application number
BR112017022724-0A
Other languages
English (en)
Chinese (zh)
Inventor
Kang Yuejing
Guo Mingxin
Liu Haili
Zhang Chengwei
Ding Derong
Jiao Guowei
Wei Xuesong
Tang Bo
Xia Zuling
Xiong Guanjun
Xu Liang
Bao Xiaoming
Original Assignee
Beijing Dabeinong Technology Group Co., Ltd.
Beijing Dabeinong Biotechnology Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beijing Dabeinong Technology Group Co., Ltd., Beijing Dabeinong Biotechnology Co., Ltd. filed Critical Beijing Dabeinong Technology Group Co., Ltd.
Publication of BR112017022724A2 publication Critical patent/BR112017022724A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

Abstract

trata-se de uma sequência de nucleotídeos para detectar um evento de maís dbn9858 que existe em uma amostra biológica, e um método de detecção do mesmo.
BR112017022724-0A 2015-04-30 2016-04-28 planta de milho tolerante a herbicida dbn9858 e sequência de nucleotídeos e método para detectar a mesma BR112017022724A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201510219911.8A CN104878095B (zh) 2015-04-30 2015-04-30 用于检测除草剂耐受性玉米植物dbn9858的核酸序列及其检测方法
CN201510219911.8 2015-04-30
PCT/CN2016/080542 WO2016173508A1 (zh) 2015-04-30 2016-04-28 除草剂耐受性玉米植物dbn9858及用于检测其的核酸序列和方法

Publications (1)

Publication Number Publication Date
BR112017022724A2 true BR112017022724A2 (pt) 2018-07-17

Family

ID=53945769

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017022724-0A BR112017022724A2 (pt) 2015-04-30 2016-04-28 planta de milho tolerante a herbicida dbn9858 e sequência de nucleotídeos e método para detectar a mesma

Country Status (8)

Country Link
CN (1) CN104878095B (pt)
AR (1) AR104436A1 (pt)
BR (1) BR112017022724A2 (pt)
PH (1) PH12017501957A1 (pt)
RU (1) RU2712730C2 (pt)
UA (1) UA127176C2 (pt)
WO (1) WO2016173508A1 (pt)
ZA (1) ZA201707935B (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104878095B (zh) * 2015-04-30 2018-10-26 北京大北农科技集团股份有限公司 用于检测除草剂耐受性玉米植物dbn9858的核酸序列及其检测方法
CN106086011B (zh) * 2016-06-18 2019-10-18 北京大北农科技集团股份有限公司 用于检测除草剂耐受性大豆植物dbn9004的核酸序列及其检测方法
CN110195122A (zh) * 2019-01-02 2019-09-03 四川省农业科学院生物技术核技术研究所 一种用于检测玉米植物t抗-4的核酸序列及其检测方法
CN112795571B (zh) * 2019-11-14 2022-09-06 中国种子集团有限公司 抗除草剂玉米转化体及其创制方法
CN112301144A (zh) * 2020-10-13 2021-02-02 中国农业科学院生物技术研究所 用于检测转基因玉米dbn9958的rpa引物探针组合、试剂盒及检测方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL129707A0 (en) * 1996-11-07 2000-02-29 Zeneca Ltd Herbicide resistant plants
US6465636B1 (en) * 1998-04-01 2002-10-15 Zeneca Mogen B.V. Pathogen-inducible promoter
EP1136560B1 (en) * 1999-09-30 2008-11-19 Japan Tobacco Inc. Vectors for transforming plants
BRPI0100752B1 (pt) * 2000-06-22 2015-10-13 Monsanto Co moléculas e pares de moléculas de dna, processos para detectar molécula de dna e para criar um traço tolerante a glifosato em plantas de milho, bem como kit de detecção de dna
MXPA05011795A (es) * 2003-05-02 2006-02-17 Dow Agrosciences Llc Evidencia de maiz tc1507 y metodos para deteccion del mismo.
EP1621629A1 (en) * 2004-07-28 2006-02-01 Expressive Research B.V. A method to increase pathogen resistance in plants
EA022829B1 (ru) * 2006-05-26 2016-03-31 Монсанто Текнолоджи, Ллс Трансгенное растение или его часть, обладающие устойчивостью к насекомым отряда lepidoptera
UA110320C2 (en) * 2008-12-16 2015-12-25 Syngenta Participations Ag Corn event 5307
WO2013169923A2 (en) * 2012-05-08 2013-11-14 Monsanto Technology Llc Corn event mon 87411
CN104878095B (zh) * 2015-04-30 2018-10-26 北京大北农科技集团股份有限公司 用于检测除草剂耐受性玉米植物dbn9858的核酸序列及其检测方法

Also Published As

Publication number Publication date
PH12017501957A1 (en) 2018-03-19
WO2016173508A1 (zh) 2016-11-03
UA127176C2 (uk) 2023-05-31
ZA201707935B (en) 2018-11-28
RU2017140978A3 (pt) 2019-05-30
AR104436A1 (es) 2017-07-19
CN104878095A (zh) 2015-09-02
CN104878095B (zh) 2018-10-26
RU2017140978A (ru) 2019-05-30
RU2712730C2 (ru) 2020-01-30

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Owner name: BEIJING DABEINONG BIOTECHNOLOGY CO., LTD. (CN)

B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]