AR104436A1 - Planta de maíz dbn9858 tolerante a herbicidas y secuencia de ácido nucleico y método para su detección - Google Patents

Planta de maíz dbn9858 tolerante a herbicidas y secuencia de ácido nucleico y método para su detección

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Publication number
AR104436A1
AR104436A1 ARP160101205A ARP160101205A AR104436A1 AR 104436 A1 AR104436 A1 AR 104436A1 AR P160101205 A ARP160101205 A AR P160101205A AR P160101205 A ARP160101205 A AR P160101205A AR 104436 A1 AR104436 A1 AR 104436A1
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Argentina
Prior art keywords
seq
nucleic acid
acid sequence
corn
sequence
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ARP160101205A
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English (en)
Inventor
Kang Yuejing
Guo Mingxin
Liu Haili
Zhang Chengwei
Ding Derong
Jiao Guowei
Wei Xuesong
Tang Bo
Xia Zuling
Xiong Guanjun
Xu Liang
Bao Xiaoming
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Beijing Dabeinong Tech Group
Beijing Dabeinong Biotechnology Co Ltd
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Publication of AR104436A1 publication Critical patent/AR104436A1/es

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    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
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Abstract

Reivindicación 1: Una molécula de ácido nucleico que tiene la siguiente secuencia de ácido nucleico, donde la secuencia de ácido nucleico comprende al menos 11 nucleótidos consecutivos en la SEQ ID Nº 3 o la secuencia complementaria de la misma, y/o al menos 11 nucleótidos consecutivos en la SEQ ID Nº 4 o la secuencia complementaria de la misma. Reivindicación 5: Un método para detectar la presencia de ADN del evento de maíz transgénico DBN9858 en una muestra, que comprende: poner en contacto la muestra a ser detectada con al menos dos iniciadores para la amplificación de un producto de amplificación de interés en una reacción de amplificación de ácido nucleico; llevara a cabo la reacción de amplificación del ácido nucleico; y detectar la presencia del producto de amplificación de interés; donde el producto de amplificación de interés comprende al menos 11 nucleótidos consecutivos en la SEQ ID Nº 3 o la secuencia complementaria de la misma, y/o al menos 11 nucleótidos consecutivos en la SEQ ID Nº 4 o la secuencia complementaria de la misma. Reivindicación 15: Un kit de detección de ADN que comprende al menos una molécula de ADN, donde la molécula de ADN comprende al menos 11 nucleótidos consecutivos en la secuencia homóloga de la SEQ ID Nº 3 o la secuencia complementaria de la misma, y/o al menos 11 nucleótidos consecutivos en la secuencia homóloga de la SEQ ID Nº 4 o la secuencia complementaria de la misma, y es útil como un iniciador de ADN o sonda específica a un evento de maíz transgénico DBN9858 o su progenie. Reivindicación 19: Un método para producir una planta de maíz que presenta tolerancia al herbicida glifosato y/o al herbicida glufosinato, que comprende: introducir una secuencia de ácido nucleico que codifica una proteína EPSPS tolerante al glifosato y/o a secuencia de ácido nucleico que codifica una proteina PAT tolerante al glufosinato y una secuencia de ácido nucleico en una región específica en el genoma de la planta de maíz, donde la secuencia de ácido nucleico en la región específica es al menos una secuencia de ácido nucleico seleccionada de las secuencias indicadas en la SEQ ID Nº 1, la SEQ ID Nº 2, la SEQ ID Nº 3, la SEQ ID Nº 4, la SEQ ID Nº 6 y la SEQ ID Nº 7; preferentemente, el método que comprende introducir la secuencia de ácido nucleico previstas en la SEQ ID Nº 5 en el genoma de la planta de maíz. Reivindicación 23: Un método para controlar malezas resistentes al glifosato en un campo de una planta tolerante al glifosato, que comprende aplicar un herbicida que comprende una dosis efectiva de glufosinato en el campo donde se planta al menos una planta de maíz transgénica tolerante al glifosato, donde la planta de maíz transgénica tolerante al glifosato comprende en su genoma al menos una secuencia de ácido nucleico seleccionada de las secuencias indicadas en la SEQ ID Nº 1, la SEQ ID Nº 2, la SEQ ID Nº 3, la SEQ ID Nº 4, la SEQ ID Nº 5, la SEQ ID Nº 6 y la SEQ ID Nº 7, y la planta de maíz transgénica tolerante al glifosato tiene asimismo tolerancia al herbicida glufosinato. Reivindicación 25: Un producto agrícola o producto básico que comprende el polinucleótido de la SEQ ID Nº 1 o la SEQ ID Nº 2, donde el producto agrícola o el producto básico es harina de maíz, maicena, aceite de maíz, almidón de maíz, gluten de maíz, torta de maíz, cosméticos o empastes.
ARP160101205A 2015-04-30 2016-04-28 Planta de maíz dbn9858 tolerante a herbicidas y secuencia de ácido nucleico y método para su detección AR104436A1 (es)

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AR (1) AR104436A1 (es)
BR (1) BR112017022724A2 (es)
PH (1) PH12017501957A1 (es)
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CN104878095B (zh) * 2015-04-30 2018-10-26 北京大北农科技集团股份有限公司 用于检测除草剂耐受性玉米植物dbn9858的核酸序列及其检测方法
CN106086011B (zh) * 2016-06-18 2019-10-18 北京大北农科技集团股份有限公司 用于检测除草剂耐受性大豆植物dbn9004的核酸序列及其检测方法
CN112795571B (zh) * 2019-11-14 2022-09-06 中国种子集团有限公司 抗除草剂玉米转化体及其创制方法
CN112301144A (zh) * 2020-10-13 2021-02-02 中国农业科学院生物技术研究所 用于检测转基因玉米dbn9958的rpa引物探针组合、试剂盒及检测方法

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RU2017140978A (ru) 2019-05-30
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PH12017501957A1 (en) 2018-03-19
CN104878095B (zh) 2018-10-26
UA127176C2 (uk) 2023-05-31
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RU2017140978A3 (es) 2019-05-30
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