AR104436A1 - DBN9858 CORN PLANT TOLERANT TO HERBICIDES AND SEQUENCE OF NUCLEIC ACID AND METHOD FOR DETECTION - Google Patents

DBN9858 CORN PLANT TOLERANT TO HERBICIDES AND SEQUENCE OF NUCLEIC ACID AND METHOD FOR DETECTION

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Publication number
AR104436A1
AR104436A1 ARP160101205A ARP160101205A AR104436A1 AR 104436 A1 AR104436 A1 AR 104436A1 AR P160101205 A ARP160101205 A AR P160101205A AR P160101205 A ARP160101205 A AR P160101205A AR 104436 A1 AR104436 A1 AR 104436A1
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AR
Argentina
Prior art keywords
seq
nucleic acid
acid sequence
corn
sequence
Prior art date
Application number
ARP160101205A
Other languages
Spanish (es)
Inventor
Kang Yuejing
Guo Mingxin
Liu Haili
Zhang Chengwei
Ding Derong
Jiao Guowei
Wei Xuesong
Tang Bo
Xia Zuling
Xiong Guanjun
Xu Liang
Bao Xiaoming
Original Assignee
Beijing Dabeinong Tech Group
Beijing Dabeinong Biotechnology Co Ltd
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Publication date
Application filed by Beijing Dabeinong Tech Group, Beijing Dabeinong Biotechnology Co Ltd filed Critical Beijing Dabeinong Tech Group
Publication of AR104436A1 publication Critical patent/AR104436A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

Abstract

Reivindicación 1: Una molécula de ácido nucleico que tiene la siguiente secuencia de ácido nucleico, donde la secuencia de ácido nucleico comprende al menos 11 nucleótidos consecutivos en la SEQ ID Nº 3 o la secuencia complementaria de la misma, y/o al menos 11 nucleótidos consecutivos en la SEQ ID Nº 4 o la secuencia complementaria de la misma. Reivindicación 5: Un método para detectar la presencia de ADN del evento de maíz transgénico DBN9858 en una muestra, que comprende: poner en contacto la muestra a ser detectada con al menos dos iniciadores para la amplificación de un producto de amplificación de interés en una reacción de amplificación de ácido nucleico; llevara a cabo la reacción de amplificación del ácido nucleico; y detectar la presencia del producto de amplificación de interés; donde el producto de amplificación de interés comprende al menos 11 nucleótidos consecutivos en la SEQ ID Nº 3 o la secuencia complementaria de la misma, y/o al menos 11 nucleótidos consecutivos en la SEQ ID Nº 4 o la secuencia complementaria de la misma. Reivindicación 15: Un kit de detección de ADN que comprende al menos una molécula de ADN, donde la molécula de ADN comprende al menos 11 nucleótidos consecutivos en la secuencia homóloga de la SEQ ID Nº 3 o la secuencia complementaria de la misma, y/o al menos 11 nucleótidos consecutivos en la secuencia homóloga de la SEQ ID Nº 4 o la secuencia complementaria de la misma, y es útil como un iniciador de ADN o sonda específica a un evento de maíz transgénico DBN9858 o su progenie. Reivindicación 19: Un método para producir una planta de maíz que presenta tolerancia al herbicida glifosato y/o al herbicida glufosinato, que comprende: introducir una secuencia de ácido nucleico que codifica una proteína EPSPS tolerante al glifosato y/o a secuencia de ácido nucleico que codifica una proteina PAT tolerante al glufosinato y una secuencia de ácido nucleico en una región específica en el genoma de la planta de maíz, donde la secuencia de ácido nucleico en la región específica es al menos una secuencia de ácido nucleico seleccionada de las secuencias indicadas en la SEQ ID Nº 1, la SEQ ID Nº 2, la SEQ ID Nº 3, la SEQ ID Nº 4, la SEQ ID Nº 6 y la SEQ ID Nº 7; preferentemente, el método que comprende introducir la secuencia de ácido nucleico previstas en la SEQ ID Nº 5 en el genoma de la planta de maíz. Reivindicación 23: Un método para controlar malezas resistentes al glifosato en un campo de una planta tolerante al glifosato, que comprende aplicar un herbicida que comprende una dosis efectiva de glufosinato en el campo donde se planta al menos una planta de maíz transgénica tolerante al glifosato, donde la planta de maíz transgénica tolerante al glifosato comprende en su genoma al menos una secuencia de ácido nucleico seleccionada de las secuencias indicadas en la SEQ ID Nº 1, la SEQ ID Nº 2, la SEQ ID Nº 3, la SEQ ID Nº 4, la SEQ ID Nº 5, la SEQ ID Nº 6 y la SEQ ID Nº 7, y la planta de maíz transgénica tolerante al glifosato tiene asimismo tolerancia al herbicida glufosinato. Reivindicación 25: Un producto agrícola o producto básico que comprende el polinucleótido de la SEQ ID Nº 1 o la SEQ ID Nº 2, donde el producto agrícola o el producto básico es harina de maíz, maicena, aceite de maíz, almidón de maíz, gluten de maíz, torta de maíz, cosméticos o empastes.Claim 1: A nucleic acid molecule having the following nucleic acid sequence, wherein the nucleic acid sequence comprises at least 11 consecutive nucleotides in SEQ ID No. 3 or the complementary sequence thereof, and / or at least 11 nucleotides consecutive in SEQ ID No. 4 or the complementary sequence thereof. Claim 5: A method for detecting the presence of DNA from the DBN9858 transgenic corn event in a sample, comprising: contacting the sample to be detected with at least two primers for amplification of an amplification product of interest in a reaction nucleic acid amplification; carry out the nucleic acid amplification reaction; and detect the presence of the amplification product of interest; wherein the amplification product of interest comprises at least 11 consecutive nucleotides in SEQ ID No. 3 or the complementary sequence thereof, and / or at least 11 consecutive nucleotides in SEQ ID No. 4 or the complementary sequence thereof. Claim 15: A DNA detection kit comprising at least one DNA molecule, wherein the DNA molecule comprises at least 11 consecutive nucleotides in the homologous sequence of SEQ ID No. 3 or the complementary sequence thereof, and / or at least 11 consecutive nucleotides in the homologous sequence of SEQ ID No. 4 or the complementary sequence thereof, and is useful as a DNA initiator or probe specific to a DBN9858 transgenic corn event or its progeny. Claim 19: A method for producing a corn plant that exhibits tolerance to glyphosate herbicide and / or glufosinate herbicide, comprising: introducing a nucleic acid sequence encoding a glyphosate-tolerant EPSPS protein and / or nucleic acid sequence encoding a glufosinate-tolerant PAT protein and a nucleic acid sequence in a specific region in the genome of the corn plant, where the nucleic acid sequence in the specific region is at least one nucleic acid sequence selected from the sequences indicated in the SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 6 and SEQ ID No. 7; preferably, the method comprising introducing the nucleic acid sequence set forth in SEQ ID No. 5 into the genome of the corn plant. Claim 23: A method of controlling glyphosate resistant weeds in a field of a glyphosate tolerant plant, which comprises applying a herbicide comprising an effective dose of glufosinate in the field where at least one transgenic glyphosate tolerant corn plant is planted, where the glyphosate tolerant transgenic corn plant comprises in its genome at least one nucleic acid sequence selected from the sequences indicated in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6 and SEQ ID No. 7, and the glyphosate tolerant transgenic corn plant also has tolerance to the herbicide glufosinate. Claim 25: An agricultural product or commodity comprising the polynucleotide of SEQ ID No. 1 or SEQ ID No. 2, wherein the agricultural product or the basic product is cornmeal, cornstarch, corn oil, corn starch, gluten of corn, corn cake, cosmetics or fillings.

ARP160101205A 2015-04-30 2016-04-28 DBN9858 CORN PLANT TOLERANT TO HERBICIDES AND SEQUENCE OF NUCLEIC ACID AND METHOD FOR DETECTION AR104436A1 (en)

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CN (1) CN104878095B (en)
AR (1) AR104436A1 (en)
BR (1) BR112017022724A2 (en)
PH (1) PH12017501957A1 (en)
RU (1) RU2712730C2 (en)
UA (1) UA127176C2 (en)
WO (1) WO2016173508A1 (en)
ZA (1) ZA201707935B (en)

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CN104878095B (en) * 2015-04-30 2018-10-26 北京大北农科技集团股份有限公司 Nucleic acid sequence and its detection method for detecting herbicide tolerant corn plant DBN9858
CN106086011B (en) * 2016-06-18 2019-10-18 北京大北农科技集团股份有限公司 For detecting the nucleic acid sequence and its detection method of herbicide-tolerant soybean plant DBN9004
CN112795571B (en) * 2019-11-14 2022-09-06 中国种子集团有限公司 Herbicide-resistant corn transformant and preparation method thereof
CN112301144A (en) * 2020-10-13 2021-02-02 中国农业科学院生物技术研究所 RPA primer probe combination, kit and detection method for detecting transgenic corn DBN9958

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110195122A (en) * 2019-01-02 2019-09-03 四川省农业科学院生物技术核技术研究所 It is a kind of for detecting the nucleic acid sequence and its detection method of corn plant T anti-4

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RU2712730C2 (en) 2020-01-30
WO2016173508A1 (en) 2016-11-03
PH12017501957A1 (en) 2018-03-19
UA127176C2 (en) 2023-05-31
BR112017022724A2 (en) 2018-07-17
ZA201707935B (en) 2018-11-28
RU2017140978A (en) 2019-05-30
RU2017140978A3 (en) 2019-05-30
CN104878095A (en) 2015-09-02
CN104878095B (en) 2018-10-26

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