AR104437A1 - CORN PLANT DBN9888 TOLERANT TO HERBICIDES AND SEQUENCE OF NUCLEIC ACID AND METHOD FOR DETECTION - Google Patents
CORN PLANT DBN9888 TOLERANT TO HERBICIDES AND SEQUENCE OF NUCLEIC ACID AND METHOD FOR DETECTIONInfo
- Publication number
- AR104437A1 AR104437A1 ARP160101206A ARP160101206A AR104437A1 AR 104437 A1 AR104437 A1 AR 104437A1 AR P160101206 A ARP160101206 A AR P160101206A AR P160101206 A ARP160101206 A AR P160101206A AR 104437 A1 AR104437 A1 AR 104437A1
- Authority
- AR
- Argentina
- Prior art keywords
- seq
- nucleic acid
- acid sequence
- corn plant
- sequence
- Prior art date
Links
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Botany (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Environmental Sciences (AREA)
- Analytical Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Forests & Forestry (AREA)
- Veterinary Medicine (AREA)
- Toxicology (AREA)
- Public Health (AREA)
- Ecology (AREA)
- Pest Control & Pesticides (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Biodiversity & Conservation Biology (AREA)
- Epidemiology (AREA)
- Insects & Arthropods (AREA)
Abstract
Reivindicación 1: Una molécula de ácido nucleico que tiene la siguiente secuencia de ácido nucleico, donde la secuencia de ácido nucleico comprende al menos 11 nucleótidos consecutivos en la SEQ ID Nº 3 o la secuencia complementaria de la misma, y/o al menos 11 nucleótidos consecutivos en la SEQ ID Nº 4 o la secuencia complementaria de la misma. Reivindicación 5: Un método para detectar la presencia de ADN del evento de maíz transgénico DBN9888 en una muestra, que comprende: poner en contacto la muestra a ser detectada con al menos dos iniciadores para la amplificación de un producto de amplificación de interés en una reacción de amplificación de ácido nucleico; llevara a cabo la reacción de amplificación del ácido nucleico; y detectar la presencia del producto de amplificación de interés; donde el producto de amplificación de interés comprende al menos 11 nucleótidos consecutivos en la SEQ ID Nº 3 o la secuencia complementaria de la misma, y/o al menos 11 nucleótidos consecutivos en la SEQ ID Nº 4 o la secuencia complementaria de la misma. Reivindicación 15: Un kit de detección de ADN que comprende al menos una molécula de ADN, donde la molécula de ADN comprende al menos 11 nucleótidos consecutivos en la secuencia homóloga de la SEQ ID Nº 3 o la secuencia complementaria de la misma, y/o al menos 11 nucleótidos consecutivos en la secuencia homóloga de la SEQ ID Nº 4 o la secuencia complementaria de la misma, y es útil como un iniciador de ADN o sonda específica a un evento de maíz transgénico DBN9888 o su progenie. Reivindicación 19: Un método para producir una planta de maíz que presenta tolerancia al herbicida glifosato y/o al herbicida glufosinato, que comprende: introducir una secuencia de ácido nucleico que codifica una proteína EPSPS tolerante al glifosato y/o a secuencia de ácido nucleico que codifica una proteína PAT tolerante al glufosinato y una secuencia de ácido nucleico en una región específica en el genoma de la planta de maíz, donde la secuencia de ácido nucleico en la región específica es al menos una secuencia de ácido nucleico seleccionada de las secuencias indicadas en la SEQ ID Nº 1, la SEQ ID Nº 2, la SEQ ID Nº 3, la SEQ ID Nº 4, la SEQ ID Nº 6 y la SEQ ID Nº 7; preferentemente, el método que comprende introducir la secuencia de ácido nucleico previstas en la SEQ ID Nº 5 en el genoma de la planta de maíz. Reivindicación 22: Un método para proteger plantas del daño provocado por el herbicida y/o para controlar las malezas en un campo, que comprende: aplicar un herbicida que comprende una dosis efectiva, de glifosato y/o glufosinato en un campo donde se planta al menos una planta de maíz transgénica, donde la planta de maíz transgénica comprende en su genoma al menos una secuencia de ácido nucleico seleccionada de las secuencias indicadas en la SEQ ID Nº 1, la SEQ ID Nº 2, la SEQ ID Nº 3, la SEQ ID Nº 4, la SEQ ID Nº 5, la SEQ ID Nº 6 y la SEQ ID Nº 7, y la planta de maíz transgénica tiene tolerancia al herbicida glifosato y/ o al herbicida glufosinato. Reivindicación 25: Un producto agrícola o producto básico que comprende el polinucleótido de la SEQ ID Nº 1 o la SEQ ID Nº 2, donde el producto agrícola o el producto básico es harina de maíz, maicena, aceite de maíz, almidón de maíz, gluten de maíz, torta de maíz, cosméticos o empastes.Claim 1: A nucleic acid molecule having the following nucleic acid sequence, wherein the nucleic acid sequence comprises at least 11 consecutive nucleotides in SEQ ID No. 3 or the complementary sequence thereof, and / or at least 11 nucleotides consecutive in SEQ ID No. 4 or the complementary sequence thereof. Claim 5: A method for detecting the presence of DNA from the DBN9888 transgenic corn event in a sample, comprising: contacting the sample to be detected with at least two primers for amplification of an amplification product of interest in a reaction nucleic acid amplification; carry out the nucleic acid amplification reaction; and detect the presence of the amplification product of interest; wherein the amplification product of interest comprises at least 11 consecutive nucleotides in SEQ ID No. 3 or the complementary sequence thereof, and / or at least 11 consecutive nucleotides in SEQ ID No. 4 or the complementary sequence thereof. Claim 15: A DNA detection kit comprising at least one DNA molecule, wherein the DNA molecule comprises at least 11 consecutive nucleotides in the homologous sequence of SEQ ID No. 3 or the complementary sequence thereof, and / or at least 11 consecutive nucleotides in the homologous sequence of SEQ ID No. 4 or the complementary sequence thereof, and is useful as a DNA initiator or probe specific to a DBN9888 transgenic corn event or its progeny. Claim 19: A method for producing a corn plant that exhibits tolerance to glyphosate herbicide and / or glufosinate herbicide, comprising: introducing a nucleic acid sequence encoding a glyphosate-tolerant EPSPS protein and / or nucleic acid sequence encoding a glufosinate-tolerant PAT protein and a nucleic acid sequence in a specific region in the genome of the corn plant, where the nucleic acid sequence in the specific region is at least one nucleic acid sequence selected from the sequences indicated in the SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 6 and SEQ ID No. 7; preferably, the method comprising introducing the nucleic acid sequence set forth in SEQ ID No. 5 into the genome of the corn plant. Claim 22: A method for protecting plants from damage caused by the herbicide and / or for controlling weeds in a field, comprising: applying a herbicide comprising an effective dose of glyphosate and / or glufosinate in a field where it is planted at less a transgenic corn plant, where the transgenic corn plant comprises in its genome at least one nucleic acid sequence selected from the sequences indicated in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6 and SEQ ID No. 7, and the transgenic corn plant has tolerance to glyphosate herbicide and / or glufosinate herbicide. Claim 25: An agricultural product or commodity comprising the polynucleotide of SEQ ID No. 1 or SEQ ID No. 2, wherein the agricultural product or the basic product is cornmeal, cornstarch, corn oil, corn starch, gluten of corn, corn cake, cosmetics or fillings.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510219905.2A CN104878094B (en) | 2015-04-30 | 2015-04-30 | For detecting the nucleic acid sequence and its detection method of herbicide tolerant corn plant DBN9888 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR104437A1 true AR104437A1 (en) | 2017-07-19 |
Family
ID=53945768
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP160101206A AR104437A1 (en) | 2015-04-30 | 2016-04-28 | CORN PLANT DBN9888 TOLERANT TO HERBICIDES AND SEQUENCE OF NUCLEIC ACID AND METHOD FOR DETECTION |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN104878094B (en) |
| AR (1) | AR104437A1 (en) |
| WO (1) | WO2016173540A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113278721A (en) * | 2021-04-27 | 2021-08-20 | 隆平生物技术(海南)有限公司 | Transgenic corn event LW2-2 and methods for detecting same |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104878094B (en) * | 2015-04-30 | 2019-01-15 | 北京大北农科技集团股份有限公司 | For detecting the nucleic acid sequence and its detection method of herbicide tolerant corn plant DBN9888 |
| CA3057917A1 (en) | 2018-10-16 | 2020-04-16 | Monsanto Technology Llc | Brassica event mon94100 and methods of use thereof |
| CN110184276A (en) * | 2019-01-02 | 2019-08-30 | 四川省农业科学院生物技术核技术研究所 | A kind of method for creating of Transgenic Resistant Herbicide corn event |
| CN112301144A (en) * | 2020-10-13 | 2021-02-02 | 中国农业科学院生物技术研究所 | RPA primer probe combination, kit and detection method for detecting transgenic corn DBN9958 |
| CN112662799B (en) * | 2020-12-24 | 2023-12-22 | 吉林省农业科学院 | Nucleic acid molecule for detecting corn plant ND4401 and detection method thereof |
| CN114015682B (en) * | 2021-11-16 | 2022-10-21 | 四川省农业科学院生物技术核技术研究所 | Specific probe, primer, kit and method for identifying nucleic acid sample |
| CN115725571A (en) * | 2022-12-13 | 2023-03-03 | 山东省农业科学院 | Nucleic acid sequence of corn transformation event LG11 and detection method thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2269666A1 (en) * | 1996-11-07 | 1998-05-14 | Zeneca Limited | Herbicide resistant plants |
| TWI243852B (en) * | 1998-06-15 | 2005-11-21 | Pharmacia & Upjohn Ab | Angiogenesis related molecules |
| KR100785946B1 (en) * | 1999-09-30 | 2007-12-14 | 니뽄 다바코 산교 가부시키가이샤 | Vectors for transforming plants |
| BRPI0100752B1 (en) * | 2000-06-22 | 2015-10-13 | Monsanto Co | DNA Molecules and Pairs of Molecules, Processes for Detecting DNA Molecules and for Creating a Glyphosate Tolerant Trait in Corn Plants, as well as DNA Detection Kit |
| ES2498976T3 (en) * | 2006-05-26 | 2014-09-26 | Monsanto Technology, Llc | Corn plant and seed corresponding to the MON89034 transgenic event and procedures for its detection and use |
| CN104878094B (en) * | 2015-04-30 | 2019-01-15 | 北京大北农科技集团股份有限公司 | For detecting the nucleic acid sequence and its detection method of herbicide tolerant corn plant DBN9888 |
-
2015
- 2015-04-30 CN CN201510219905.2A patent/CN104878094B/en active Active
-
2016
- 2016-04-28 AR ARP160101206A patent/AR104437A1/en unknown
- 2016-04-29 WO PCT/CN2016/080677 patent/WO2016173540A1/en active Application Filing
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113278721A (en) * | 2021-04-27 | 2021-08-20 | 隆平生物技术(海南)有限公司 | Transgenic corn event LW2-2 and methods for detecting same |
| CN113278721B (en) * | 2021-04-27 | 2023-08-15 | 隆平生物技术(海南)有限公司 | Transgenic corn event LW2-2 and detection method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2016173540A1 (en) | 2016-11-03 |
| CN104878094B (en) | 2019-01-15 |
| CN104878094A (en) | 2015-09-02 |
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