AR105554A1 - CORN PLANT DBN9936 AND NUCLEIC ACID SEQUENCES AND METHODS FOR DETECTION OF THE SAME - Google Patents

CORN PLANT DBN9936 AND NUCLEIC ACID SEQUENCES AND METHODS FOR DETECTION OF THE SAME

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Publication number
AR105554A1
AR105554A1 ARP160101198A ARP160101198A AR105554A1 AR 105554 A1 AR105554 A1 AR 105554A1 AR P160101198 A ARP160101198 A AR P160101198A AR P160101198 A ARP160101198 A AR P160101198A AR 105554 A1 AR105554 A1 AR 105554A1
Authority
AR
Argentina
Prior art keywords
seq
nucleic acid
corn
acid sequence
plant
Prior art date
Application number
ARP160101198A
Other languages
Spanish (es)
Inventor
Guo Hanzi
Huang Jincun
Jia Zhiwei
Wang Lijun
Pang Jie
Liu Haili
Zhang Yunzhu
Kang Yuejing
Ding Derong
Zhang Shiping
Lu Yuping
Bao Xiaoming
Li Feng
Zhou Yi
Fu Xueqian
Wang Lei
Original Assignee
Beijing Dabeinong Tech Group
Beijing Dabeinong Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beijing Dabeinong Tech Group, Beijing Dabeinong Biotechnology Co Ltd filed Critical Beijing Dabeinong Tech Group
Publication of AR105554A1 publication Critical patent/AR105554A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/32—Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
    • A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Abstract

Reivindicación 1: Una secuencia de ácido nucleico, caracterizada porque comprende al menos 11 nucleótidos consecutivos de SEQ ID Nº 3 o una secuencia complementaria de la misma, y/o al menos 11 nucleótidos consecutivos de SEQ ID Nº 4 o una secuencia complementaria de la misma. Reivindicación 5: Un método para detectar la presencia del ADN del evento de maíz transgénico DBN9936 en una muestra, que comprende: poner en contacto la muestra con al menos dos cebadores en una reacción de amplificación de ácido nucleico; realizar la reacción de amplificación de ácido nucleico; y detectar la presencia de un amplicón, en el que la presencia del amplicón indica la presencia del ADN del evento de maíz transgénico DBN9936 en la muestra; en el que el amplicón comprende al menos 11 nucleótidos consecutivos de la SEQ ID Nº 3 o una secuencia complementaria de la misma, o al menos 11 nucleótidos consecutivos de la SEQ ID Nº 4 o una secuencia complementaria de la misma; y preferiblemente, el amplicón comprende los nucleótidos consecutivos 1 - 11 ó 12 - 22 de la SEQ ID Nº 1 o una secuencia complementaria de la misma, o los nucleótidos consecutivos 1-11 o 12-22 de la SEQ ID Nº 2 o una secuencia complementaria de la misma; preferiblemente, el amplicón comprende la SEQ ID Nº 1 o una secuencia complementaria de la misma, SEQ ID Nº 2 o una secuencia complementaria de la misma, SEQ ID Nº 6 o una secuencia complementaria de la misma, o SEQ ID Nº 7 o una secuencia complementaria de la misma. Reivindicación 11: Una célula o parte de planta, caracterizada porque comprende una secuencia de ácido nucleico que codifica la proteína Cry1Ab resistente a los insectos, una secuencia de ácido nucleico que codifica la proteína EPSPS tolerante a herbicidas de glifosato y una secuencia de ácido nucleico de una región específica, en el que la secuencia de ácido nucleico de una región específica comprende la SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 6 o SEQ ID Nº 7; preferiblemente, la célula o parte de la planta de maíz comprende una secuencia de ácido nucleico que codifica la proteína Cry1Ab resistente a los insectos, una secuencia de ácido nucleico que codifica la proteína EPSPS de tolerancia a herbicidas de glifosato y una secuencia de ácido nucleico de una región específica, en el que la secuencia de ácido nucleico de una región específica comprende la SEQ ID Nº 3 o SEQ ID Nº 4; preferiblemente, la célula o parte de la planta de maíz comprende la secuencia de ácido nucleico tal como se establecen la SEQ ID Nº 5. Reivindicación 12: Un método para proteger una planta de maíz de la invasión de insectos, caracterizado porque comprende proporcionar al menos una célula o parte de la planta de maíz transgénico de la reivindicación 11 en la dieta del insecto diana, y la ingestión de la célula o parte de la planta de maíz transgénico inhibe al insecto diana de alimentarse aún mas de la planta de maíz. Reivindicación 15: Un método para cultivar una planta de maíz tolerante a herbicidas de glifosato, caracterizado porque comprende: plantar al menos una semilla de maíz, en el que la semilla de maíz comprende en su genoma una secuencia de ácido nucleico que codifica la proteína EPSPS tolerante a los herbicidas de glifosato y una secuencia de ácido nucleico de una región específica; crecer la semilla de maíz en una planta de maíz; y pulverizar la planta de maíz con una cantidad eficaz de herbicida de glifosato, y a continuación recoger la planta con una reducción de daños en la planta, en comparación con otras plantas que no comprenden la secuencia de ácido nucleico de la región específica; en el que la secuencia de ácido nucleico de la región específica se selecciona entre al menos una secuencia de ácido nucleico que consiste en la SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 3, SEQ ID Nº 4, SEQ ID Nº 6 y SEQ ID Nº 7; preferiblemente, el método comprende: plantar al menos una semilla de maíz, en el que la semilla de maíz comprende en su genoma la secuencia de ácido nucleico tal como se establece en la SEQ ID Nº 5; crecer la semilla de maíz en una planta de maíz; y pulverizar la planta de maíz con una cantidad eficaz de herbicida de glifosato, y a continuación recoger la planta con una reducción de daños en la planta, en comparación con otras plantas que no comprenden la SEQ ID Nº 5. Reivindicación 19: Un método para producir una planta de maíz resistente a los insectos y tolerante a herbicidas de glifosato, caracterizado porque comprende: cruzar sexualmente una primera planta de maíz parental tolerante a herbicidas de glifosato y resistente a los insectos del evento de maíz transgénico DBN9936, con una segunda planta de maíz parental que carece de la característica de tolerancia a glifosato y/o resistencia a los insectos, produciendo de ese modo una pluralidad de plantas de la progenie; tratar las plantas de la progenie con herbicida de glifosato; y seleccionar las plantas de la progenie tolerantes a glifosato, que también son resistentes al daño por alimentación de un insecto; en el que el evento de maíz transgénico DBN9936 comprende en su genoma al menos una secuencia de ácido nucleico seleccionada del grupo que consiste en la SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 3, SEQ ID Nº 4, SEQ ID Nº 5, SEQ ID Nº 6 y SEQ ID Nº 7. Reivindicación 21: Un producto o materia prima agrícola que comprende los polinucleótidos tal como se establecen en la SEQ ID Nº 1 o SEQ ID Nº 2, caracterizado porque el producto o materia agrícola es polenta, harina de maíz, aceite de maíz, almidón de maíz, gluten de maíz, tortas de maíz, un cosmético o un agente de carga.Claim 1: A nucleic acid sequence, characterized in that it comprises at least 11 consecutive nucleotides of SEQ ID No. 3 or a complementary sequence thereof, and / or at least 11 consecutive nucleotides of SEQ ID No. 4 or a complementary sequence thereof. . Claim 5: A method of detecting the presence of the DNA of the DBN9936 transgenic corn event in a sample, comprising: contacting the sample with at least two primers in a nucleic acid amplification reaction; perform the nucleic acid amplification reaction; and detect the presence of an amplicon, in which the presence of the amplicon indicates the presence of the DNA of the DBN9936 transgenic corn event in the sample; wherein the amplicon comprises at least 11 consecutive nucleotides of SEQ ID No. 3 or a complementary sequence thereof, or at least 11 consecutive nucleotides of SEQ ID No. 4 or a complementary sequence thereof; and preferably, the amplicon comprises consecutive nucleotides 1-11 or 12-22 of SEQ ID No. 1 or a complementary sequence thereof, or consecutive nucleotides 1-11 or 12-22 of SEQ ID No. 2 or a sequence. complementary to it; preferably, the amplicon comprises SEQ ID No. 1 or a complementary sequence thereof, SEQ ID No. 2 or a complementary sequence thereof, SEQ ID No. 6 or a complementary sequence thereof, or SEQ ID No. 7 or a sequence. complementary to it. Claim 11: A plant cell or part, characterized in that it comprises a nucleic acid sequence encoding insect-resistant Cry1Ab protein, a nucleic acid sequence encoding glyphosate herbicide-tolerant EPSPS protein and a nucleic acid sequence of a specific region, in which the nucleic acid sequence of a specific region comprises SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 6 or SEQ ID No. 7; preferably, the cell or part of the corn plant comprises a nucleic acid sequence encoding the insect-resistant Cry1Ab protein, a nucleic acid sequence encoding the glyphosate herbicide tolerance EPSPS protein and a nucleic acid sequence of a specific region, in which the nucleic acid sequence of a specific region comprises SEQ ID No. 3 or SEQ ID No. 4; preferably, the cell or part of the corn plant comprises the nucleic acid sequence as set forth in SEQ ID No. 5. Claim 12: A method of protecting a corn plant from insect invasion, characterized in that it comprises providing at least a cell or part of the transgenic corn plant of claim 11 in the diet of the target insect, and ingestion of the cell or part of the transgenic corn plant inhibits the target insect from feeding even more on the corn plant. Claim 15: A method of growing a glyphosate herbicide tolerant corn plant, characterized in that it comprises: planting at least one corn seed, wherein the corn seed comprises in its genome a nucleic acid sequence encoding the EPSPS protein tolerant to glyphosate herbicides and a nucleic acid sequence of a specific region; grow the corn seed in a corn plant; and pulverize the corn plant with an effective amount of glyphosate herbicide, and then collect the plant with a reduction in plant damage, compared to other plants that do not comprise the nucleic acid sequence of the specific region; wherein the nucleic acid sequence of the specific region is selected from at least one nucleic acid sequence consisting of SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 6 and SEQ ID No. 7; preferably, the method comprises: planting at least one corn seed, in which the corn seed comprises in its genome the nucleic acid sequence as set forth in SEQ ID No. 5; grow the corn seed in a corn plant; and pulverize the corn plant with an effective amount of glyphosate herbicide, and then collect the plant with a reduction in plant damage, compared to other plants that do not comprise SEQ ID No. 5. Claim 19: A method of producing an insect resistant and glyphosate herbicide-tolerant corn plant, characterized in that it comprises: sexually crossing a first plant of parental glyphosate-tolerant and insect-resistant corn from the DBN9936 transgenic corn event, with a second corn plant parental that lacks the characteristic of glyphosate tolerance and / or resistance to insects, thereby producing a plurality of progeny plants; treat progeny plants with glyphosate herbicide; and select glyphosate tolerant progeny plants, which are also resistant to damage by feeding an insect; wherein the DBN9936 transgenic corn event comprises in its genome at least one nucleic acid sequence selected from the group consisting of SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6 and SEQ ID No. 7. Claim 21: An agricultural product or raw material comprising the polynucleotides as set forth in SEQ ID No. 1 or SEQ ID No. 2, characterized in that the agricultural product or material is polenta , cornmeal, corn oil, corn starch, corn gluten, corn cakes, a cosmetic or a loading agent.

ARP160101198A 2015-04-30 2016-04-28 CORN PLANT DBN9936 AND NUCLEIC ACID SEQUENCES AND METHODS FOR DETECTION OF THE SAME AR105554A1 (en)

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CN (1) CN104830847B (en)
AR (1) AR105554A1 (en)
BR (1) BR112017023304A2 (en)
PH (1) PH12017501967A1 (en)
RU (1) RU2707527C2 (en)
UA (1) UA127944C2 (en)
WO (1) WO2016173361A1 (en)

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CN110184276A (en) * 2019-01-02 2019-08-30 四川省农业科学院生物技术核技术研究所 A kind of method for creating of Transgenic Resistant Herbicide corn event
CN109971880B (en) * 2019-04-09 2022-11-04 北京大北农生物技术有限公司 Nucleic acid sequence for detecting corn plant DBN9508 and detection method thereof
CN112831584B (en) * 2021-01-27 2023-06-06 隆平生物技术(海南)有限公司 Transgenic corn event LP007-2 and detection method thereof
CN113151533B (en) * 2021-01-27 2023-08-08 隆平生物技术(海南)有限公司 Transgenic corn event LP007-6 and detection method thereof
CN112877454B (en) * 2021-01-27 2023-08-08 隆平生物技术(海南)有限公司 Transgenic corn event LP007-3 and detection method thereof
CN113722519B (en) * 2021-09-06 2022-07-26 南京农业大学 Crop phenotype cloud platform analysis system and method
CN116144672B (en) * 2022-09-23 2023-11-07 隆平生物技术(海南)有限公司 Transgenic corn event LP026-1 and detection method thereof
CN116144817B (en) * 2022-09-23 2023-08-15 隆平生物技术(海南)有限公司 Transgenic corn event LP026-4 and detection method thereof
CN115725571A (en) * 2022-12-13 2023-03-03 山东省农业科学院 Nucleic acid sequence of corn transformation event LG11 and detection method thereof
CN116640761B (en) * 2023-07-20 2023-09-22 隆平生物技术(海南)有限公司 Transgenic maize event LP018-1 and detection method thereof
CN116694629B (en) * 2023-07-31 2023-09-29 隆平生物技术(海南)有限公司 Transgenic corn event LP038-1 and detection method thereof
CN116732062B (en) * 2023-08-08 2023-10-10 隆平生物技术(海南)有限公司 Transgenic corn event LP059-2 and detection method thereof

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BR112017023304A2 (en) 2018-10-23
CN104830847B (en) 2019-01-11
CN104830847A (en) 2015-08-12
RU2017137790A3 (en) 2019-05-30
RU2707527C2 (en) 2019-11-27
RU2017137790A (en) 2019-05-30
WO2016173361A1 (en) 2016-11-03
PH12017501967A1 (en) 2018-03-19

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