AR104430A1 - CORN PLANT DBN9978 AND NUCLEIC ACID SEQUENCES AND METHODS FOR DETECTION OF THE SAME - Google Patents

CORN PLANT DBN9978 AND NUCLEIC ACID SEQUENCES AND METHODS FOR DETECTION OF THE SAME

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Publication number
AR104430A1
AR104430A1 ARP160101199A ARP160101199A AR104430A1 AR 104430 A1 AR104430 A1 AR 104430A1 AR P160101199 A ARP160101199 A AR P160101199A AR P160101199 A ARP160101199 A AR P160101199A AR 104430 A1 AR104430 A1 AR 104430A1
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Argentina
Prior art keywords
seq
nucleic acid
acid sequence
corn
plant
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ARP160101199A
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Spanish (es)
Inventor
Guo Hanzi
Huang Jincun
Jia Zhiwei
Wang Lijun
Pang Jie
Liu Haili
Zhang Yunzhu
Kang Yuejing
Ding Derong
Zhang Shiping
Lu Yuping
Bao Xiaoming
Li Feng
Zhou Yi
Fu Xueqian
Wang Lei
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Beijing Dabeinong Tech Group
Beijing Dabeinong Biotechnology Co Ltd
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Publication of AR104430A1 publication Critical patent/AR104430A1/en

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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

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  • Proteomics, Peptides & Aminoacids (AREA)
  • Environmental Sciences (AREA)
  • Forests & Forestry (AREA)
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  • Pest Control & Pesticides (AREA)
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  • Agronomy & Crop Science (AREA)
  • Mycology (AREA)
  • Insects & Arthropods (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Abstract

Reivindicación 1: Una secuencia de ácido nucleico, caracterizada porque comprende al menos 11 nucleótidos consecutivos de SEQ ID Nº 3 o una secuencia complementaria de la misma, y/o al menos 11 nucleótidos consecutivos de SEQ ID Nº 4 o una secuencia complementaria de la misma. Reivindicación 5: Un método para detectar la presencia del ADN del evento de maíz transgénico DBN9978 en una muestra, que comprende: poner en contacto la muestra con al menos dos cebadores en una reacción de amplificación de ácido nucleico; realizar la reacción de amplificación de ácido nucleico; y detectar la presencia de un amplicón, en el que la presencia del amplicón indica la presencia del ADN del evento de maíz transgénico DBN9978 en la muestra; en el que el amplicón comprende al menos 11 nucleótidos consecutivos de la SEQ ID Nº 3 o una secuencia complementaria de la misma, o al menos 11 nucleótidos consecutivos de la SEQ ID Nº 4 o una secuencia complementaria de la misma; y preferiblemente, el amplicón comprende los nucleótidos consecutivos 1 - 11 ó 12 - 22 de la SEQ ID Nº 1 o una secuencia complementaria de la misma, o los nucleótidos consecutivos 1 - 11 ó 12 - 22 de la SEQ ID Nº 2 o una secuencia complementaria de la misma; preferiblemente, el amplicón comprende la SEQ ID Nº 1 o una secuencia complementaria de la misma, SEQ ID Nº 2 o una secuencia complementaria de la misma, SEQ ID Nº 6 o una secuencia complementaria de la misma, o SEQ ID Nº 7 o una secuencia complementaria de la misma. Reivindicación 11: Una célula o parte de planta, caracterizada porque comprende una secuencia de ácido nucleico que codifica la proteína Cry1Ab resistente a los insectos, una secuencia de ácido nucleico que codifica la proteína EPSPS tolerante a herbicidas de glifosato y una secuencia de ácido nucleico de una región específica, en el que la secuencia de ácido nucleico de una región específica comprende la SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 6 o SEQ ID Nº 7; preferiblemente, la célula o parte de la planta de maíz comprende una secuencia de ácido nucleico que codifica la proteína Cry1Ab resistente a los insectos, una secuencia de ácido nucleico que codifica la proteína EPSPS de tolerancia a herbicidas de glifosato y una secuencia de ácido nucleico de una región específica, en el que la secuencia de ácido nucleico de una región específica comprende la SEQ ID Nº 3 o SEQ ID Nº 4; preferiblemente, la célula o parte de la planta de maíz comprende la secuencia de ácido nucleico tal como se establece en la SEQ ID Nº 5. Reivindicación 12: Un método para proteger una planta de maíz de la invasión de insectos, caracterizado porque comprende proporcionar al menos una célula o parte de la planta de maíz transgénico de la reivindicación 11 en la dieta del insecto diana, y la ingestión de la célula o parte de la planta de maíz transgénico inhibe al insecto diana de alimentarse aún más de la planta de maíz. Reivindicación 15: Un método para cultivar una planta de maíz tolerante a herbicidas de glifosato, caracterizado porque comprende: plantar al menos una semilla de maíz, en el que la semilla de maíz comprende en su genoma una secuencia de ácido nucleico que codifica la proteína EPSPS tolerante a los herbicidas de glifosato y una secuencia de ácido nucleico de una región específica; crecer la semilla de maíz en una planta de maíz; y pulverizar la planta de maíz con una cantidad eficaz de herbicida de glifosato, y a continuación recoger la planta con una reducción de daños en la planta, en comparación con otras plantas que no comprenden la secuencia de ácido nucleico de la región específica; en el que la secuencia de ácido nucleico de la región específica se selecciona entre al menos una secuencia de ácido nucleico que consiste en la SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 3, SEQ ID Nº 4, SEQ ID Nº 6 y SEQ ID Nº 7; preferiblemente, el método comprende: plantar al menos una semilla de maíz, en el que la semilla de maíz comprende en su genoma la secuencia de ácido nucleico tal como se establece en la SEQ ID Nº 5; crecer la semilla de maíz en una planta de maíz; y pulverizar la planta de maíz con una cantidad eficaz de herbicida de glifosato, y a continuación recoger la planta con una reducción de daños en la planta, en comparación con otras plantas que no comprenden la SEQ ID Nº 5. Reivindicación 19: Un método para producir una planta de maíz resistente a los insectos y tolerante a herbicidas de glifosato, caracterizado porque comprende cruzar sexualmente una primera planta de maíz parental tolerante a herbicidas de glifosato y resistente a los insectos del evento de maíz transgénicoDBN9978, con una segunda planta de maíz parental que carece de la característica de tolerancia a glifosato y/o resistencia a los insectos, produciendo de ese modo una pluralidad de plantas de la progenie; tratar las plantas de la progenie con herbicida de glifosato; y seleccionar las plantas de la progenie tolerantes a glifosato, que también son resistentes al daño por alimentación de un insecto; en el que el evento de maíz transgénico DBN9978 comprende en su genoma al menos una secuencia de ácido nucleico seleccionada del grupo que consiste en la SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 3, SEQ ID Nº 4, SEQ ID Nº 5, SEQ ID Nº 6 y SEQ ID Nº 7. Reivindicación 21: Un producto o materia prima agrícola que comprende los polinucleótidos tal come se establecen en la SEQ ID Nº 1 o SEQ ID Nº 2, caracterizado porque el producto o materia agrícola es polenta, harina de maíz, aceite de maíz, almidón de maíz, gluten de maíz, tortas de maíz, un cosmético o un agente de carga.Claim 1: A nucleic acid sequence, characterized in that it comprises at least 11 consecutive nucleotides of SEQ ID No. 3 or a complementary sequence thereof, and / or at least 11 consecutive nucleotides of SEQ ID No. 4 or a complementary sequence thereof. . Claim 5: A method for detecting the presence of the DNA of the DBN9978 transgenic corn event in a sample, comprising: contacting the sample with at least two primers in a nucleic acid amplification reaction; perform the nucleic acid amplification reaction; and detect the presence of an amplicon, in which the presence of the amplicon indicates the presence of the DNA of the DBN9978 transgenic corn event in the sample; wherein the amplicon comprises at least 11 consecutive nucleotides of SEQ ID No. 3 or a complementary sequence thereof, or at least 11 consecutive nucleotides of SEQ ID No. 4 or a complementary sequence thereof; and preferably, the amplicon comprises consecutive nucleotides 1-11 or 12-22 of SEQ ID No. 1 or a complementary sequence thereof, or consecutive nucleotides 1-11 or 12-22 of SEQ ID No. 2 or a sequence. complementary to it; preferably, the amplicon comprises SEQ ID No. 1 or a complementary sequence thereof, SEQ ID No. 2 or a complementary sequence thereof, SEQ ID No. 6 or a complementary sequence thereof, or SEQ ID No. 7 or a sequence. complementary to it. Claim 11: A plant cell or part, characterized in that it comprises a nucleic acid sequence encoding insect-resistant Cry1Ab protein, a nucleic acid sequence encoding glyphosate herbicide-tolerant EPSPS protein and a nucleic acid sequence of a specific region, in which the nucleic acid sequence of a specific region comprises SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 6 or SEQ ID No. 7; preferably, the cell or part of the corn plant comprises a nucleic acid sequence encoding the insect-resistant Cry1Ab protein, a nucleic acid sequence encoding the glyphosate herbicide tolerance EPSPS protein and a nucleic acid sequence of a specific region, in which the nucleic acid sequence of a specific region comprises SEQ ID No. 3 or SEQ ID No. 4; preferably, the cell or part of the corn plant comprises the nucleic acid sequence as set forth in SEQ ID No. 5. Claim 12: A method of protecting a corn plant from insect invasion, characterized in that it comprises providing the at least one cell or part of the transgenic corn plant of claim 11 in the diet of the target insect, and ingestion of the cell or part of the transgenic corn plant inhibits the target insect from feeding even more on the corn plant. Claim 15: A method of growing a glyphosate herbicide tolerant corn plant, characterized in that it comprises: planting at least one corn seed, wherein the corn seed comprises in its genome a nucleic acid sequence encoding the EPSPS protein tolerant to glyphosate herbicides and a nucleic acid sequence of a specific region; grow the corn seed in a corn plant; and pulverize the corn plant with an effective amount of glyphosate herbicide, and then collect the plant with a reduction in plant damage, compared to other plants that do not comprise the nucleic acid sequence of the specific region; wherein the nucleic acid sequence of the specific region is selected from at least one nucleic acid sequence consisting of SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 6 and SEQ ID No. 7; preferably, the method comprises: planting at least one corn seed, in which the corn seed comprises in its genome the nucleic acid sequence as set forth in SEQ ID No. 5; grow the corn seed in a corn plant; and pulverize the corn plant with an effective amount of glyphosate herbicide, and then collect the plant with a reduction in plant damage, compared to other plants that do not comprise SEQ ID No. 5. Claim 19: A method of producing an insect resistant and glyphosate herbicide tolerant corn plant, characterized in that it comprises sexually crossing a first glyphosate tolerant and insect resistant parental corn plant of the transgenic corn event DBN9978, with a second parental corn plant that it lacks the characteristic of glyphosate tolerance and / or insect resistance, thereby producing a plurality of progeny plants; treat progeny plants with glyphosate herbicide; and select glyphosate tolerant progeny plants, which are also resistant to damage by feeding an insect; wherein the DBN9978 transgenic corn event comprises in its genome at least one nucleic acid sequence selected from the group consisting of SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6 and SEQ ID No. 7. Claim 21: An agricultural product or raw material comprising the polynucleotides as established in SEQ ID No. 1 or SEQ ID No. 2, characterized in that the agricultural product or material is polenta , cornmeal, corn oil, corn starch, corn gluten, corn cakes, a cosmetic or a loading agent.

ARP160101199A 2015-04-30 2016-04-28 CORN PLANT DBN9978 AND NUCLEIC ACID SEQUENCES AND METHODS FOR DETECTION OF THE SAME AR104430A1 (en)

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