AR108750A1 - SEQUENCE OF NUCLEIC ACIDS FOR THE DETECTION OF SOIL TOLERANT SOIL PLANT DBN9008 AND ITS DETECTION METHOD - Google Patents

SEQUENCE OF NUCLEIC ACIDS FOR THE DETECTION OF SOIL TOLERANT SOIL PLANT DBN9008 AND ITS DETECTION METHOD

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Publication number
AR108750A1
AR108750A1 ARP170101646A ARP170101646A AR108750A1 AR 108750 A1 AR108750 A1 AR 108750A1 AR P170101646 A ARP170101646 A AR P170101646A AR P170101646 A ARP170101646 A AR P170101646A AR 108750 A1 AR108750 A1 AR 108750A1
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AR
Argentina
Prior art keywords
seq
nucleic acid
acid sequence
sequence
plant
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ARP170101646A
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Spanish (es)
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Beijing Dabeinong Tech Group
Beijing Dabeinong Biotechnology Co Ltd
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Application filed by Beijing Dabeinong Tech Group, Beijing Dabeinong Biotechnology Co Ltd filed Critical Beijing Dabeinong Tech Group
Publication of AR108750A1 publication Critical patent/AR108750A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/32—Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
    • A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01183—Phosphinothricin acetyltransferase (2.3.1.183)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01019—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
    • A01H6/542—Glycine max [soybean]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La presente divulgación se refiere a una secuencia de ácidos nucleicos para la detección de una planta de soja tolerante a herbicida DBN9008 y su método de detección, la secuencia de ácidos nucleicos de la planta de soja comprende la SEQ ID Nº 1 o su secuencia complementaria o la SEQ ID Nº 2 o su secuencia complementaria. El evento de soja transgénica DBN9008 tiene mejor tolerancia a un herbicida de glifosato y un herbicida de glufosinato y no tiene influencia sobre el rendimiento y el método de detección puede identificar de modo preciso y rápido si una molécula de ADN del evento de soja transgénico DBN9008 está contenida en una muestra biológica. Reivindicación 19: El kit de detección de ADN de acuerdo con la reivindicación 17 ó 18, en donde la molécula de ADN comprende al menos una seleccionada de: una secuencia homóloga de la SEQ ID Nº 1 o su secuencia complementaria, una secuencia homóloga de la SEQ ID Nº 2 o su secuencia complementaria, una secuencia homóloga de la SEQ ID Nº 6 o su secuencia complementaria y una secuencia homóloga de la SEQ ID Nº 7 o su secuencia complementaria. Reivindicación 20: Una célula de planta o parte, en donde comprende una secuencia de ácidos nucleicos que codifica una proteína EPSPS tolerante a glifosato, una secuencia de ácidos nucleicos que codifica una proteína PAT tolerante a glufosinato y una secuencia de ácidos nucleicos de una región específica, donde la secuencia de ácidos nucleicos de la región específica comprende al menos una seleccionada de las secuencias como se muestra en la SEQ ID Nº 1, la SEQ ID Nº 2, la SEQ ID Nº 6 y la SEQ ID Nº 7. Reivindicación 21: Un método para producir una planta de soja tolerante a un herbicida de glifosato y/o un herbicida de glufosinato, que comprende introducir en el genoma de la planta de soja una secuencia de ácidos nucleicos que codifica una proteína EPSPS tolerante a glifosato y/o una secuencia de ácidos nucleicos que codifica una proteína PAT tolerante a glufosinato y una secuencia de ácidos nucleicos de una región específica selecciona de al menos una secuencia de ácidos nucleicos de las secuencias como se muestra en la SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 3, SEQ ID Nº 4, SEQ ID Nº 6 y SEQ ID Nº 7; con preferencia, que comprende introducir en el genoma de la planta de soja una secuencia de ácidos nucleicos como se muestra en la SEQ ID Nº 5. Reivindicación 25: Un método de control de malezas resistentes a glifosato en un campo para una planta tolerante a glifosato, que comprende la aplicación de un herbicida que contiene una dosis efectiva de glufosinato a un campo para plantar al menos una planta de soja transgénica tolerante a glifosato, en donde la planta de soja transgénica tolerante a glifosato comprende al menos una secuencia de ácidos nucleicos seleccionada de las secuencias como se muestra en la SEQ ID Nº 1, la SEQ ID Nº 2, la SEQ ID Nº 3, la SEQ ID Nº 4, la SEQ ID Nº 5, la SEQ ID Nº 6 y la SEQ ID Nº 7 en su genoma y la planta de soja transgénica tolerante a glifosato tiene tolerancia al herbicida de glufosinato al mismo tiempo. Reivindicación 27: Un producto agrícola o mercadería que comprende un polinucleótido de la SEQ ID Nº 1 o la SEQ ID Nº 2, en donde el producto agrícola o mercadería es lecitina, un ácido graso, glicerol, un esterol, un copo de soja, harina de soja, una proteína de soja o su concentrado, un aceite de soja, una fibra de proteína de soja, un coágulo de leche de soja o cuajada de fríjol.The present disclosure relates to a nucleic acid sequence for the detection of a herbicide tolerant soybean plant DBN9008 and its detection method, the nucleic acid sequence of the soybean plant comprises SEQ ID No. 1 or its complementary sequence or SEQ ID No. 2 or its complementary sequence. The DBN9008 GM soybean event has better tolerance to a glyphosate herbicide and a glufosinate herbicide and has no influence on performance and the detection method can identify precisely and quickly if a DNA molecule from the DBN9008 GM soybean is contained in a biological sample. Claim 19: The DNA detection kit according to claim 17 or 18, wherein the DNA molecule comprises at least one selected from: a homologous sequence of SEQ ID No. 1 or its complementary sequence, a homologous sequence of the SEQ ID No. 2 or its complementary sequence, a homologous sequence of SEQ ID No. 6 or its complementary sequence and a homologous sequence of SEQ ID No. 7 or its complementary sequence. Claim 20: A plant or part cell, wherein it comprises a nucleic acid sequence encoding a glyphosate tolerant EPSPS protein, a nucleic acid sequence encoding a glufosinate tolerant PAT protein and a nucleic acid sequence of a specific region , wherein the nucleic acid sequence of the specific region comprises at least one selected from the sequences as shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 6 and SEQ ID No. 7. Claim 21: A method of producing a glyphosate herbicide tolerant plant and / or a glufosinate herbicide, which comprises introducing a nucleic acid sequence into the genome of the soybean plant encoding a glyphosate tolerant EPSPS protein and / or a nucleic acid sequence encoding a glufosinate tolerant PAT protein and a nucleic acid sequence of a specific region selected from at least one sequence Nucleic acid ia of the sequences as shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 6 and SEQ ID No. 7; preferably, which comprises introducing a nucleic acid sequence into the soybean genome as shown in SEQ ID No. 5. Claim 25: A glyphosate resistant weed control method in a field for a glyphosate tolerant plant , which comprises the application of a herbicide containing an effective dose of glufosinate to a field to plant at least one glyphosate tolerant transgenic soybean plant, wherein the glyphosate tolerant transgenic soybean plant comprises at least one selected nucleic acid sequence of the sequences as shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6 and SEQ ID No. 7 in its genome and the glyphosate tolerant transgenic soybean plant has tolerance to glufosinate herbicide at the same time. Claim 27: An agricultural product or merchandise comprising a polynucleotide of SEQ ID No. 1 or SEQ ID No. 2, wherein the agricultural product or merchandise is lecithin, a fatty acid, glycerol, a sterol, a soybean, flour of soybeans, a soy protein or its concentrate, a soybean oil, a soy protein fiber, a clot of soy milk or bean curd.

ARP170101646A 2016-06-18 2017-06-15 SEQUENCE OF NUCLEIC ACIDS FOR THE DETECTION OF SOIL TOLERANT SOIL PLANT DBN9008 AND ITS DETECTION METHOD AR108750A1 (en)

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CN106086011B (en) * 2016-06-18 2019-10-18 北京大北农科技集团股份有限公司 For detecting the nucleic acid sequence and its detection method of herbicide-tolerant soybean plant DBN9004
CN106086010B (en) * 2016-06-18 2019-10-18 北京大北农科技集团股份有限公司 For detecting the nucleic acid sequence and its detection method of herbicide-tolerant soybean plant DBN9008
WO2021026689A1 (en) * 2019-08-09 2021-02-18 北京大北农生物技术有限公司 Nucleic acid sequence used for detecting soybean plant dbn8007 and detection method therefor
AU2019460919B2 (en) * 2019-08-09 2024-03-21 Beijing Dabeinong Biotechnology Co., Ltd. Nucleic acid sequence for detecting soybean plant DBN8002 and detection method therefor
CN112359123A (en) * 2020-10-10 2021-02-12 浙江省农业科学院 Real-time fluorescence quantitative detection method for g10evo-epsps transgenic soybean and kit thereof
CN114787389A (en) * 2022-02-21 2022-07-22 北京大北农生物技术有限公司 Nucleic acid sequence for detecting soybean plant DBN8205 and detection method thereof
CN116656869B (en) * 2023-07-24 2023-09-22 捷康生物科技(海南)有限公司 Transgenic soybean event JK1001-2 and detection method thereof
CN116694812B (en) * 2023-07-25 2023-10-03 隆平生物技术(海南)有限公司 Transgenic soybean event LP086-2 and detection method thereof
CN116694813B (en) * 2023-07-25 2023-10-03 隆平生物技术(海南)有限公司 Transgenic soybean event LP086-1 and detection method thereof
CN116694814B (en) * 2023-08-01 2023-10-10 隆平生物技术(海南)有限公司 Transgenic soybean event LP012-1 and detection method thereof
CN116694815B (en) * 2023-08-01 2023-10-03 隆平生物技术(海南)有限公司 Transgenic soybean event LP012-2 and detection method thereof

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CN106086010B (en) * 2016-06-18 2019-10-18 北京大北农科技集团股份有限公司 For detecting the nucleic acid sequence and its detection method of herbicide-tolerant soybean plant DBN9008

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