AR104438A1 - CORN PLANT DBN9868 TOLERANT TO HERBICIDES AND SEQUENCE OF NUCLEIC ACID AND METHOD FOR DETECTION - Google Patents

CORN PLANT DBN9868 TOLERANT TO HERBICIDES AND SEQUENCE OF NUCLEIC ACID AND METHOD FOR DETECTION

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Publication number
AR104438A1
AR104438A1 ARP160101207A ARP160101207A AR104438A1 AR 104438 A1 AR104438 A1 AR 104438A1 AR P160101207 A ARP160101207 A AR P160101207A AR P160101207 A ARP160101207 A AR P160101207A AR 104438 A1 AR104438 A1 AR 104438A1
Authority
AR
Argentina
Prior art keywords
dbn9868
nucleic acid
detection
corn plant
sequence
Prior art date
Application number
ARP160101207A
Other languages
Spanish (es)
Inventor
Bao Xiaoming
Xu Liang
Xiong Guanjun
Xia Zuling
Tang Bo
Wei Xuesong
Jiao Guowei
Ding Derong
Zhang Chengwei
Liu Haili
Guo Mingxin
Kang Yuejing
Original Assignee
Beijing Dabeinong Tech Group
Beijing Dabeinong Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beijing Dabeinong Tech Group, Beijing Dabeinong Biotechnology Co Ltd filed Critical Beijing Dabeinong Tech Group
Publication of AR104438A1 publication Critical patent/AR104438A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/32—Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
    • A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

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  • Life Sciences & Earth Sciences (AREA)
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  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Immunology (AREA)
  • Pest Control & Pesticides (AREA)
  • Botany (AREA)
  • Agronomy & Crop Science (AREA)
  • Cell Biology (AREA)
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  • Biodiversity & Conservation Biology (AREA)
  • Insects & Arthropods (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La presente se refiere a una secuencia de ácido nucleico para detectar la planta de maíz DBN9868 tolerante a herbicidas y un método para su detección, donde la secuencia de ácido nucleico de la planta de maíz comprende la SEQ ID Nº 1 o la secuencia complementaria de la misma, o la SEQ ID Nº 2 o la secuencia complementaria de la misma. El evento de maíz transgénico DBN9868 de la presente tiene mejor tolerancia al herbicida glifosato y al herbicida glufosinato sin afectar el rendimiento, y el método de detección puede detectar precisa y rápidamente si una muestra biológica comprende la molécula de ADN del evento de maíz transgénico DBN9868.This refers to a nucleic acid sequence for detecting the herbicide-tolerant DBN9868 corn plant and a method for its detection, where the nucleic acid sequence of the corn plant comprises SEQ ID No. 1 or the complementary sequence of the same, or SEQ ID No. 2 or the complementary sequence thereof. The DBN9868 transgenic corn event here has better tolerance to glyphosate herbicide and glufosinate herbicide without affecting performance, and the detection method can accurately and quickly detect if a biological sample comprises the DNA molecule of the DBN9868 transgenic corn event.

ARP160101207A 2015-04-30 2016-04-28 CORN PLANT DBN9868 TOLERANT TO HERBICIDES AND SEQUENCE OF NUCLEIC ACID AND METHOD FOR DETECTION AR104438A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510219912.2A CN104878096B (en) 2015-04-30 2015-04-30 For detecting the nucleic acid sequence and its detection method of herbicide tolerant corn plant DBN9868

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AR104438A1 true AR104438A1 (en) 2017-07-19

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CN (1) CN104878096B (en)
AR (1) AR104438A1 (en)
WO (1) WO2016173539A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104878096B (en) * 2015-04-30 2019-01-15 北京大北农科技集团股份有限公司 For detecting the nucleic acid sequence and its detection method of herbicide tolerant corn plant DBN9868
CN109868273B (en) * 2019-04-09 2023-02-03 北京大北农生物技术有限公司 Nucleic acid sequence for detecting corn plant DBN9501 and detection method thereof
WO2021026689A1 (en) * 2019-08-09 2021-02-18 北京大北农生物技术有限公司 Nucleic acid sequence used for detecting soybean plant dbn8007 and detection method therefor
CN112831584B (en) * 2021-01-27 2023-06-06 隆平生物技术(海南)有限公司 Transgenic corn event LP007-2 and detection method thereof
CN112852991B (en) * 2021-01-27 2023-08-04 隆平生物技术(海南)有限公司 Transgenic corn event LP007-7 and detection method thereof
CN113201531B (en) * 2021-04-27 2023-06-27 隆平生物技术(海南)有限公司 Transgenic corn event LW2-1 and detection method thereof

Family Cites Families (7)

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Publication number Priority date Publication date Assignee Title
EP0946737A2 (en) * 1996-11-07 1999-10-06 Zeneca Limited Herbicide resistant plants
BR122013026754B1 (en) * 2000-06-22 2018-02-27 Monsanto Company DNA Molecule And Processes To Produce A Corn Plant Tolerant For Glyphosate Herbicide Application
KR20090033840A (en) * 2006-05-26 2009-04-06 몬산토 테크놀로지 엘엘씨 Corn plant and seed corresponding to transgenic event mon89034 and methods for detection and use thereof
CN101641443A (en) * 2006-10-30 2010-02-03 先锋高级育种国际公司 Maize event DP-098140-6 and compositions and methods for the identification and/or detection thereof
CN103827305B (en) * 2011-07-28 2017-07-04 吉奈克蒂夫公司 Glyphosate tolerant corn event VCO Φ 1,981 5 and the kit detected for it and method
WO2014004458A2 (en) * 2012-06-25 2014-01-03 Dow Agrosciences Llc Insect resistant and herbicide tolerant soybean event pdab9582.816.15.1
CN104878096B (en) * 2015-04-30 2019-01-15 北京大北农科技集团股份有限公司 For detecting the nucleic acid sequence and its detection method of herbicide tolerant corn plant DBN9868

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CN104878096B (en) 2019-01-15
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