EA201401195A1 - SET OF REAGENTS FOR CHLAMYDIA TRACHOMATIS DNA RECOGNITION AND ITS APPLICATION - Google Patents

SET OF REAGENTS FOR CHLAMYDIA TRACHOMATIS DNA RECOGNITION AND ITS APPLICATION

Info

Publication number
EA201401195A1
EA201401195A1 EA201401195A EA201401195A EA201401195A1 EA 201401195 A1 EA201401195 A1 EA 201401195A1 EA 201401195 A EA201401195 A EA 201401195A EA 201401195 A EA201401195 A EA 201401195A EA 201401195 A1 EA201401195 A1 EA 201401195A1
Authority
EA
Eurasian Patent Office
Prior art keywords
chlamydia trachomatis
chain reaction
polymerase chain
reagents
trachomatis dna
Prior art date
Application number
EA201401195A
Other languages
Russian (ru)
Other versions
EA028686B1 (en
Inventor
Александр Евгеньевич Гущин
Герман Александрович Шипулин
Original Assignee
Общество с ограниченной ответственностью "ИнтерЛабСервис"
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Общество с ограниченной ответственностью "ИнтерЛабСервис" filed Critical Общество с ограниченной ответственностью "ИнтерЛабСервис"
Priority to EA201401195A priority Critical patent/EA028686B1/en
Priority to RU2014151193A priority patent/RU2621863C2/en
Publication of EA201401195A1 publication Critical patent/EA201401195A1/en
Publication of EA028686B1 publication Critical patent/EA028686B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Abstract

Группа изобретений относится к медицине, более конкретно - к лабораторной диагностике, и может быть использована для выявления ДНК Chlamydia trachomatis в биологических образцах методом полимеразной цепной реакции. Предложенный набор реагентов включает по меньшей мере два олигонуклеотидных праймера для полимеразной цепной реакции, обеспечивающих специфическую амплификацию фрагмента ДНК Chlamydia trachomatis, лежащего внутри участка криптической плазмиды Chlamydia trachomatis последовательности SEQ ID NO:1. Размер амплифицируемого фрагмента составляет от 70 до 110 пар нуклеотидов. Также предложено применение описанного набора реагентов для выявления ДНК Chlamydia trachomatis в биологических образцах. Группа изобретений позволяет с высокой чувствительностью и специфичностью выявлять все клинически значимые штаммы/генотипы/серовары урогенитального биовара Chlamydia trachomatis с помощью единственной мишени полимеразной цепной реакции.The group of inventions relates to medicine, more specifically to laboratory diagnostics, and can be used to detect Chlamydia trachomatis DNA in biological samples by the method of polymerase chain reaction. The proposed reagent kit includes at least two oligonucleotide primers for the polymerase chain reaction that provide specific amplification of the Chlamydia trachomatis DNA fragment, which lies within the region of the Chlamydia trachomatis cryptic plasmid of the sequence SEQ ID NO: 1. The size of the amplified fragment ranges from 70 to 110 base pairs. Also proposed the use of the described set of reagents for the detection of Chlamydia trachomatis DNA in biological samples. The group of inventions allows to identify all clinically significant strains / genotypes / serovars of the urogenital biovar Chlamydia trachomatis with high sensitivity and specificity using a single target polymerase chain reaction.

EA201401195A 2014-11-14 2014-11-14 Reagent kit for detection of chlamydia trachomatis dna and application thereof EA028686B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EA201401195A EA028686B1 (en) 2014-11-14 2014-11-14 Reagent kit for detection of chlamydia trachomatis dna and application thereof
RU2014151193A RU2621863C2 (en) 2014-11-14 2014-12-18 Reagent kit for chlamydia trachomatis dna detection and its application

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EA201401195A EA028686B1 (en) 2014-11-14 2014-11-14 Reagent kit for detection of chlamydia trachomatis dna and application thereof

Publications (2)

Publication Number Publication Date
EA201401195A1 true EA201401195A1 (en) 2016-05-31
EA028686B1 EA028686B1 (en) 2017-12-29

Family

ID=56080128

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201401195A EA028686B1 (en) 2014-11-14 2014-11-14 Reagent kit for detection of chlamydia trachomatis dna and application thereof

Country Status (2)

Country Link
EA (1) EA028686B1 (en)
RU (1) RU2621863C2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110923345B (en) * 2019-12-19 2023-06-27 武汉中帜生物科技股份有限公司 Colloidal gold chromatography kit for chlamydia trachomatis detection and application thereof

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1224253B (en) * 1988-04-08 1990-09-26 Sclavo Spa SYNTHETIC OLIGONUCLEOTIDES USEFUL FOR THE DETERMINATION OF CHLAMYDIA TRACHOMATIS IN A BIOLOGICAL SAMPLE
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
RU2245369C1 (en) * 2003-05-05 2005-01-27 Смоленская государственная медицинская академия Method for differential diagnosis of representatives of family chlamydiaceae
US20070065837A1 (en) * 2005-09-21 2007-03-22 Qiagen Diagnostics Gmbh Methods and compositions for the detection of Chlamydia trachomatis
WO2007056398A2 (en) * 2005-11-07 2007-05-18 Siemens Healthcare Diagnostics Inc. Chlamydia trachomatis specific oligonucleotide sequences
RU2385945C1 (en) * 2008-12-26 2010-04-10 Федеральное государственное учреждение науки "Московский научно-исследовательский институт эпидемиологии и микробиологии имени Г.Н. Габричевского Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека" (ФГУН МНИИЭМ им. Г.Н. Габричевского Роспотребнадзора) Method for prediction of human or simian manifested or obliterated chlamidia infection and kit for implementation thereof
RU2385946C1 (en) * 2008-12-26 2010-04-10 Федеральное государственное учреждение науки "Московский научно-исследовательский институт эпидемиологии и микробиологии имени Г.Н. Габричевского Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека" (ФГУН МНИИЭМ им. Г.Н. Габричевского Роспотребнадзора) Diagnostic technique for human or simian chlamidia infection and kit for implementation thereof
RU2443782C1 (en) * 2010-08-03 2012-02-27 Федеральное государственное учреждение науки "Московский научно-исследовательский институт эпидемиологии и микробиологии имени Г.Н. Габричевского Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека" (ФГУН МНИИЭМ им. Г.Н. Габричевского Роспотребнадзора) Method for chlamydia trachomatis genotyping

Also Published As

Publication number Publication date
EA028686B1 (en) 2017-12-29
RU2621863C2 (en) 2017-06-07
RU2014151193A (en) 2016-07-10

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