AR124132A2 - Métodos para identificar simultáneamente dos eventos elite en plantas de soja con tolerancia a herbicidas, par de cebadores, método para confirmar pureza de semillas comprendiendo dichos eventos elite y par de moléculas de adn - Google Patents

Métodos para identificar simultáneamente dos eventos elite en plantas de soja con tolerancia a herbicidas, par de cebadores, método para confirmar pureza de semillas comprendiendo dichos eventos elite y par de moléculas de adn

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Publication number
AR124132A2
AR124132A2 ARP210103238A ARP210103238A AR124132A2 AR 124132 A2 AR124132 A2 AR 124132A2 AR P210103238 A ARP210103238 A AR P210103238A AR P210103238 A ARP210103238 A AR P210103238A AR 124132 A2 AR124132 A2 AR 124132A2
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Argentina
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nucleotide
seq
specific
ncimb
deposit number
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ARP210103238A
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English (en)
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Justin Thomas Mason
Leslie James Lettow
Mark Alan Eby
William H Eby
Gnter Welz
Steven Verhaeghe
Beuckeleer Marc De
Veerle Habex
Jean-Marc Ferullo
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BASF Agricultural Solutions Seed US LLC
Ms Tech Llc
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Publication of AR124132A2 publication Critical patent/AR124132A2/es

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    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
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    • C07H21/04Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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  • Pretreatment Of Seeds And Plants (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Plantas, material vegetal y semillas de soja transgénica específica, caracterizados porque estos productos contienen una pila de eventos de transformación específicos en una localización específica en el genoma del soja. También se proporcionan herramientas que permiten la identificación rápida e inequívoca de estos eventos en muestras biológicas. Reivindicación 1: Un método para identificar la presencia simultánea del evento élite depositado en el NCIMB con el número de depósito NCIMB 41659 y el evento élite depositado en el NCIMB con el número de depósito NCIMB 41660 en muestras biológicas, donde dicho método se caracteriza por la detección de regiones específicas de evento con una sonda específica para cada evento que reconoce específicamente la región flanqueadora 5’ o 3’ del ADN extraño que comprende un gen de tolerancia a herbicidas en cada evento, y el ADN extraño contiguo al mismo, dicho método comprende: hibridizar un ácido nucleico de muestras biológicas con una primera sonda específica para el evento elite depositado en el NCIMB con el número de depósito NCIMB 41659, en el que dicha primera sonda comprende la secuencia de SEQ ID Nº 2 desde el nucleótido 1431 hasta el nucleótido 1472 o SEQ ID Nº 3 desde el nucleótido 220 hasta el nucléotido 261 o el complemento de dichas secuencias, e hibridizar un ácido nucleico de muestras biológicas con una segunda sonda específica para el evento elite depositado en el NCIMB con el número de depósito NCIMB 41660, en donde dicha segunda sonda comprende la secuencia de SEQ ID Nº 14 desde el nucleótido 301 hasta el nucleótido 322 o SEQ ID Nº 15 desde el nucleótido 497 hasta el nucleótido 518, o el complemento de dichas secuencias, en donde el evento elite depositado en el NCIMB con el número de depósito NCIMB 41659 contiene los genes quiméricos 2mEPSPS y HPPD-Pf W336 y el evento elite depositado en el NCIMB con el número de depósito NCIMB 41660 contiene el gen de fosfinotricina acetiltransferasa quimérico, excluyendo dichas muestras biológicas del alcance de esta reivindicación. Reivindicación 4: Un par de moléculas de ácido nucleico aisladas específicas para los eventos elite de la reivindicación 1, caracterizado porque la primer molécula de ácido nucleico comprende la secuencia de nucleótidos de la SEQ ID Nº 2 desde el nucleótido 1441 hasta el nucleótido 1452 o a la SEQ ID Nº 3 desde el nucleótido 230 hasta el nucleótido 251, o el complemento de dichas secuencias, y la segunda molécula de ácido nucleico comprende la secuencia de nucleótidos de la SEQ ID Nº 14 desde el nucleótido 301 hasta el nucleótido 322 o a la SEQ ID Nº 15 desde el nucleótido 497 hasta el nucleótido 518, o el complemento de dichas secuencias.
ARP210103238A 2009-11-23 2021-11-24 Métodos para identificar simultáneamente dos eventos elite en plantas de soja con tolerancia a herbicidas, par de cebadores, método para confirmar pureza de semillas comprendiendo dichos eventos elite y par de moléculas de adn AR124132A2 (es)

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EP09014565 2009-11-23

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AR124132A2 true AR124132A2 (es) 2023-02-15

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ARP100104306A AR080566A1 (es) 2009-11-23 2010-11-23 Plantas de soja con tolerancia a herbicidas y metodos para identificarlas
ARP210103238A AR124132A2 (es) 2009-11-23 2021-11-24 Métodos para identificar simultáneamente dos eventos elite en plantas de soja con tolerancia a herbicidas, par de cebadores, método para confirmar pureza de semillas comprendiendo dichos eventos elite y par de moléculas de adn

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US (2) US9062324B2 (es)
EP (1) EP2503873B1 (es)
JP (2) JP2013511294A (es)
KR (1) KR101992494B1 (es)
CN (2) CN106399482B (es)
AR (2) AR080566A1 (es)
AU (1) AU2010321586C1 (es)
CA (1) CA2781598C (es)
ES (1) ES2623923T3 (es)
HR (1) HRP20170670T1 (es)
HU (1) HUE032718T2 (es)
MX (1) MX2012005932A (es)
NZ (1) NZ600375A (es)
PL (1) PL2503873T3 (es)
PT (1) PT2503873T (es)
RS (1) RS55883B1 (es)
RU (1) RU2639520C2 (es)
SI (1) SI2503873T1 (es)
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WO (1) WO2011063413A2 (es)

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NZ600375A (en) 2014-10-31
RU2012125961A (ru) 2013-12-27
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