ES2896198T3 - Cebadores y sondas de reacción en cadena de polimerasa para Mycobacterium tuberculosis - Google Patents

Cebadores y sondas de reacción en cadena de polimerasa para Mycobacterium tuberculosis Download PDF

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ES2896198T3
ES2896198T3 ES15848780T ES15848780T ES2896198T3 ES 2896198 T3 ES2896198 T3 ES 2896198T3 ES 15848780 T ES15848780 T ES 15848780T ES 15848780 T ES15848780 T ES 15848780T ES 2896198 T3 ES2896198 T3 ES 2896198T3
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mycobacterium tuberculosis
probes
chain reaction
polymerase chain
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David Alland
Soumitesh Chakravorty
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Rutgers State University of New Jersey
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
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Abstract

Un kit para detectar Mycobacterium tuberculosis farmacorresistente múltiple en una muestra, comprendiendo el kit: un primer par de cebadores directo e inverso para amplificar una primera parte de una primera región, siendo la primera región el gen rrs de Mycobacterium tuberculosis, en donde cada cebador para el gen rrs tiene una secuencia que es al menos un 85 % idéntica a una secuencia oligonucleotídica seleccionada entre el grupo que consiste en las SEQ ID NO: 16, 51 y 52, y un segundo par de cebadores directo e inverso para amplificar una segunda parte de una segunda región de un gen rpoB; y una sonda de ácido nucleico aislado que comprende una secuencia que es al menos un 85 % idéntica a SEQ ID NO: 59.
ES15848780T 2014-10-10 2015-10-09 Cebadores y sondas de reacción en cadena de polimerasa para Mycobacterium tuberculosis Active ES2896198T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462062351P 2014-10-10 2014-10-10
PCT/US2015/054916 WO2016057905A1 (en) 2014-10-10 2015-10-09 Polymerase chain reaction primers and probes for mycobacterium tuberculosis

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ES2896198T3 true ES2896198T3 (es) 2022-02-24

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US (2) US11180816B2 (es)
EP (2) EP3957753A1 (es)
JP (3) JP6835712B2 (es)
CN (2) CN116042880A (es)
AU (1) AU2015330738B2 (es)
BR (1) BR112017007229A2 (es)
CA (1) CA2964265A1 (es)
ES (1) ES2896198T3 (es)
LT (1) LT3204517T (es)
PT (1) PT3204517T (es)
RU (1) RU2717655C2 (es)
WO (1) WO2016057905A1 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JOP20160086B1 (ar) 2015-05-08 2021-08-17 2 Katholieke Univ Leuven Ku Leuven Research And Development مشتقات اندول مستبدلة احاديا او ثنائيا بصفتها مانعات للتكاثر الفيروسي لحمى الفنك
CN107557435B (zh) * 2017-10-08 2021-04-06 福建医科大学 MC-ARMS-mMB三联技术在耐药结核菌诊断试剂盒的制备方法和应用
KR101962831B1 (ko) * 2017-11-13 2019-03-27 연세대학교 원주산학협력단 퀀타매트릭스 어세이 플랫폼 기반 결핵균 및 비결핵항산균 검출 및 결핵균의 리팜핀, 아이소니아지드, 퀴놀론계, 아미노글라이코사이드계 항생제항생제 내성여부를 동시 확인할 수 있는 진단법 및 그 키트
CN108192989A (zh) * 2018-03-28 2018-06-22 首都医科大学附属北京胸科医院 用于检测结核分枝杆菌和结核病的成套试剂与试剂盒
KR102030005B1 (ko) * 2018-05-24 2019-11-08 연세대학교 원주산학협력단 개선된 퀀타매트릭스 어세이 플랫폼 기반 결핵균 검출 및 결핵균의 리팜핀, 아이소니아지드, 에탐부톨, 퀴놀론, 아미카신, 카나마이신, 카프레오마이신와 같은 이차 주사제 및 스트렙토마이신 약제에 대한 내성여부를 동시 확인할 수 있는 진단법 및 그 키트
US20230175043A1 (en) * 2019-05-03 2023-06-08 Meso Scale Technologies, Llc. Kits for detecting one or more target nucleic acid analytes in a sample and methods of making and using the same
MD20220040A2 (ro) * 2020-02-06 2023-02-28 The Administrators Of The Tulane Educational Fund Analiză bazată pe CRISPR pentru detectarea TB în fluidele corporale
CN112481399B (zh) * 2020-12-21 2022-08-30 上海国际旅行卫生保健中心(上海海关口岸门诊部) 一种基于二代测序技术用于结核分枝杆菌mlst分型的引物组及建库方法
CN112680534B (zh) * 2021-01-21 2022-10-28 哈尔滨医科大学 一种用于识别假阳性反应的结核分枝杆菌sRNA荧光定量PCR标准品及其应用
CN112725486A (zh) * 2021-02-03 2021-04-30 四川大学华西医院 一种结核分枝杆菌复合群快速检测诊断试剂盒及制备方法
CN115992272B (zh) * 2022-10-17 2023-08-04 杭州遂真生物技术有限公司 用于结核分枝杆菌及其耐药基因检测的组合物及一体式试剂盒
CN117070645A (zh) * 2023-07-25 2023-11-17 鲲鹏基因（北京）科技有限责任公司 检测结核分枝杆菌氨基糖苷耐药的组合物、试剂盒及方法

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6060237A (en) 1985-02-26 2000-05-09 Biostar, Inc. Devices and methods for optical detection of nucleic acid hybridization
US5541308A (en) * 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5652106A (en) 1993-06-04 1997-07-29 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Rapid amplification-based subtyping of mycobacterium tuberculosis
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
JP4558105B2 (ja) * 1994-06-09 2010-10-06 イノジェネティックス・ナムローゼ・フェンノートシャップ ミコバクテリウム種の抗生物質耐性スペクトルの検出方法
US6235479B1 (en) * 1999-04-13 2001-05-22 Bio Merieux, Inc. Methods and devices for performing analysis of a nucleic acid sample
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
EP1076099A3 (en) * 1999-08-03 2003-03-05 Nisshinbo Industries, Inc. Kit for diagnosis of tubercle bacilli
JP2001103981A (ja) * 1999-08-03 2001-04-17 Nisshinbo Ind Inc 結核菌診断キット
JP4919568B2 (ja) 1999-10-22 2012-04-18 ピーエイチアールアイ プロパティーズ，インコーポレイテッド 短い配列の変異体を検出するためのアッセイ
CN1151270C (zh) 2000-10-31 2004-05-26 亚能生物技术（深圳）有限公司 诊断结核杆菌及其耐药性的dna芯片
CN1153969C (zh) 2001-04-13 2004-06-16 中国科学院上海冶金研究所 结核分支杆菌耐药性检测芯片及其制作方法和应用方法
US7226732B2 (en) * 2001-07-16 2007-06-05 Cepheid Methods, apparatus, and computer programs for verifying the integrity of a probe
US20110111389A1 (en) 2001-11-07 2011-05-12 Diagcor Bioscience Incorporation Limited Rapid genotyping analysis for human papillomavirus and the device thereof
AU2003299867A1 (en) 2002-12-27 2004-07-29 University Of Medicine And Dentistry Of New Jersey Method for single nucleotide polymorphism detection
GB0403039D0 (en) * 2004-02-11 2004-03-17 Health Prot Agency TB resistance assay
ES2359322T3 (es) 2004-10-18 2011-05-20 Brandeis University Procedimiento para la amplificación de ácidos nucleicos.
CN101168767A (zh) 2006-10-25 2008-04-30 复旦大学附属华山医院 一种快速检测结核分支杆菌多重耐药性的试剂盒
CN102149822A (zh) 2008-09-12 2011-08-10 株式会社Lg生命科学 用于检测结核分枝杆菌复合群或分枝杆菌属的组合物和使用所述组合物通过多重实时pcr来同时检测结核分枝杆菌复合群和分枝杆菌属的方法
CN101580879B (zh) 2009-04-30 2012-04-11 广西医科大学 检测结核杆菌耐药基因膜芯片
WO2011140237A2 (en) * 2010-05-04 2011-11-10 The Government Of The Usa Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Process for detection of multidrug resistant tuberculosis using real-time pcr and high resolution melt analysis
CN101845503B (zh) * 2010-06-10 2013-02-06 无锡锐奇基因生物科技有限公司 检测耐药结核分枝杆菌(mdr-tb)的试剂盒
KR101261292B1 (ko) 2010-06-28 2013-05-06 주식회사 인트론바이오테크놀로지 실시간 ｐｃｒ을 이용한 미코박테리움 속 균종의 동시 감별 방법 및 이를 위한 키트
WO2012014116A1 (en) * 2010-07-29 2012-02-02 Bigtec Private Limited Probes and primers for detection of dengue
CN103562405B (zh) 2010-11-10 2018-01-09 布兰代斯大学 用于检测和鉴定分枝杆菌属的组合物、方法和试剂盒
CN103045716B (zh) 2011-10-11 2015-04-08 上海市肺科医院 焦磷酸测序技术检测结核分枝杆菌异烟肼耐药性的方法
CN103184270A (zh) * 2011-12-28 2013-07-03 北京宏微特斯生物科技有限公司 检测结核分枝杆菌（tb）的耐药基因突变型的方法和试剂盒
CN102559916B (zh) * 2012-02-24 2014-04-16 孙爱华 一种结核分枝杆菌耐多药检测方法
WO2013155189A1 (en) 2012-04-10 2013-10-17 University Of Medicine And Dentistry Of New Jersey Detection of drug resistant mycobacterium tuberculosis
TWM467671U (zh) 2012-09-14 2013-12-11 Fooyin University Hospital 結核病快速診斷及藥效檢測結構
RU127074U1 (ru) 2012-12-27 2013-04-20 Государственное бюджетное образовательное учреждение высшего профессионального образования Первый Московский государственный медицинский университет им. И.М. Сеченова Министерства здравоохранения Российской Федерации (ГБОУ ВПО Первый МГМУ им. И.М. Сеченова Минздрава России) Молекулярно-генетическая тест-система для экспресс-определения устойчивости микобактерий туберкулеза к противотуберкулезным препаратам группы фторхинолонов, аминогликозидам (амикацину, канамицину) и капреомицину
CN106906306B (zh) * 2013-03-15 2020-07-10 达雅高生命科技有限公司 快速并灵敏基因型鉴定及核酸检测

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JP2024026210A (ja) 2024-02-28
EP3204517B1 (en) 2021-08-11
LT3204517T (lt) 2021-11-25
JP6835712B2 (ja) 2021-02-24
JP2017530710A (ja) 2017-10-19
WO2016057905A1 (en) 2016-04-14
US20220064715A1 (en) 2022-03-03
AU2015330738A1 (en) 2017-05-04
RU2017113727A (ru) 2018-11-13
EP3957753A1 (en) 2022-02-23
EP3204517A1 (en) 2017-08-16
BR112017007229A2 (pt) 2021-02-09
US20170247747A1 (en) 2017-08-31
JP2020168007A (ja) 2020-10-15
PT3204517T (pt) 2021-11-16
RU2017113727A3 (es) 2019-05-20
AU2015330738B2 (en) 2021-10-14
CA2964265A1 (en) 2016-04-14
EP3204517A4 (en) 2018-07-04
JP7432237B2 (ja) 2024-02-16
CN116042880A (zh) 2023-05-02
CN107002148A (zh) 2017-08-01
US11180816B2 (en) 2021-11-23
CN107002148B (zh) 2023-02-17
RU2717655C2 (ru) 2020-03-24

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