MX2017015673A - Kit para detectar conjuntamente multiples acidos nucleicos con especificidad de objetivo que difieren entre si y metodo de deteccion utilizando el mismo. - Google Patents

Kit para detectar conjuntamente multiples acidos nucleicos con especificidad de objetivo que difieren entre si y metodo de deteccion utilizando el mismo.

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Publication number
MX2017015673A
MX2017015673A MX2017015673A MX2017015673A MX2017015673A MX 2017015673 A MX2017015673 A MX 2017015673A MX 2017015673 A MX2017015673 A MX 2017015673A MX 2017015673 A MX2017015673 A MX 2017015673A MX 2017015673 A MX2017015673 A MX 2017015673A
Authority
MX
Mexico
Prior art keywords
target nucleic
nucleic acid
target
binding
nucleic acids
Prior art date
Application number
MX2017015673A
Other languages
English (en)
Inventor
Nagano Takashi
MIYAJIMA Kensuke
Narahara Kenji
Original Assignee
Mizuho Medy Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mizuho Medy Co Ltd filed Critical Mizuho Medy Co Ltd
Publication of MX2017015673A publication Critical patent/MX2017015673A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se proporciona un kit para detectar múltiples ácidos nucleicos con especificidad de objetivo, que sean capaces de amplificar y detectar simultáneamente múltiples genes por medio de un recipiente de reacción que contenga un tipo de solución de reacción y un tipo de marcadores. La solución puede contener un primer ácido nucleico con especificidad de objetivo (10) y un segundo ácido nucleico con especificidad de objetivo (20) de los cuales cada uno se disocia a la temperatura de desnaturalización T0. La solución contiene adicionalmente: ADN polimerasa (30); un cebador del primer objetivo (13) a temperatura de alineamiento T1 que se une con las primeras cadenas simples derivadas del primer ácido nucleico con especificidad de objetivo; un cebador del segundo objetivo (23) a temperatura de alineamiento T1 con segundas cadenas simples derivadas del segundo ácido nucleico con especificidad de objetivo; una sonda del primer objetivo (15) a temperatura de alineamiento T1 que se une con las primeras cadenas simples derivadas del primer ácido nucleico con especificidad de objetivo; y una sonda del segundo objetivo (25) a una temperatura de detección del segundo objetivo T3 que es menor que la temperatura de alineamiento T1 y la temperatura de elongación T2 uniéndose con las segundas cadenas simples derivadas del segundo ácido nucleico con especificidad de objetivo. A condición de que: se cumpla que T0 sea mayor que T2; T2 no sea menor que T1; y T1 sea mayor que T3. Figura que se seleccionará: Figura 36.
MX2017015673A 2015-06-04 2016-05-10 Kit para detectar conjuntamente multiples acidos nucleicos con especificidad de objetivo que difieren entre si y metodo de deteccion utilizando el mismo. MX2017015673A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2015113979 2015-06-04
PCT/JP2016/063831 WO2016194552A1 (ja) 2015-06-04 2016-05-10 複数の標的核酸の検出キット及びそれを用いる検出方法

Publications (1)

Publication Number Publication Date
MX2017015673A true MX2017015673A (es) 2018-06-19

Family

ID=57439990

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017015673A MX2017015673A (es) 2015-06-04 2016-05-10 Kit para detectar conjuntamente multiples acidos nucleicos con especificidad de objetivo que difieren entre si y metodo de deteccion utilizando el mismo.

Country Status (16)

Country Link
US (2) US20180155764A1 (es)
EP (1) EP3305912B1 (es)
JP (1) JP6720161B2 (es)
KR (1) KR102077577B1 (es)
CN (1) CN107849617B (es)
AU (1) AU2016269875B2 (es)
BR (1) BR112017026001A2 (es)
CA (1) CA2983428C (es)
DK (1) DK3305912T3 (es)
ES (1) ES2899826T3 (es)
MX (1) MX2017015673A (es)
MY (1) MY182821A (es)
PH (1) PH12017502015A1 (es)
SG (1) SG11201708920PA (es)
TW (1) TWI686481B (es)
WO (1) WO2016194552A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
CA3095112A1 (en) * 2018-04-20 2019-10-24 Seegene, Inc. Method and apparatus for detecting a plurality of target nucleic acid sequences in sample
JP7221491B2 (ja) * 2018-09-18 2023-02-14 株式会社ミズホメディー 複数の標的核酸を検出するキットを用いる検出方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4724380Y1 (es) 1969-11-20 1972-08-01
ATE295427T1 (de) 1996-06-04 2005-05-15 Univ Utah Res Found Überwachung der hybridisierung während pcr
WO2002008414A1 (fr) * 2000-06-27 2002-01-31 National Institute Of Advanced Industrial Science And Technology Nouvelles sondes d'acides nucléiques et procédés d'essai d'acide nucléique par utilisation des mêmes
JP2002136300A (ja) 2000-08-25 2002-05-14 Otsuka Pharmaceut Co Ltd 白血病キメラ遺伝子の検出方法
JP4460228B2 (ja) 2002-04-22 2010-05-12 日鉄環境エンジニアリング株式会社 核酸の新規測定方法
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
EP1411133B1 (en) * 2002-09-24 2009-01-07 Qiagen GmbH Enhanced coamplification of nucleic acids
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
EP2116614A1 (en) * 2008-05-06 2009-11-11 Qiagen GmbH Simultaneous detection of multiple nucleic acid sequences in a reaction
WO2010068576A1 (en) * 2008-12-10 2010-06-17 Smiths Detection Inc. Identification and differentiation of nucleic acid sequence using temperature-dependent hybridization
CN102439171B (zh) * 2008-12-22 2017-07-14 犹他大学研究基金会 单色多重定量pcr
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
CN101624629B (zh) * 2009-07-24 2012-09-05 上海浩源生物科技有限公司 单管中多重靶核酸的pcr检测方法及其试剂盒
GB201100150D0 (en) * 2011-01-06 2011-02-23 Epistem Ltd Mutational analysis
JP5863162B2 (ja) * 2011-06-17 2016-02-16 広島県 標的核酸の検出方法
US10876160B2 (en) * 2011-10-31 2020-12-29 Eiken Kagaku Kabushiki Kaisha Method for detecting target nucleic acid

Also Published As

Publication number Publication date
MY182821A (en) 2021-02-05
KR20170139150A (ko) 2017-12-18
JPWO2016194552A1 (ja) 2018-03-22
EP3305912B1 (en) 2021-10-06
AU2016269875B2 (en) 2019-05-09
BR112017026001A2 (pt) 2018-11-06
CA2983428C (en) 2021-12-07
ES2899826T3 (es) 2022-03-14
CA2983428A1 (en) 2016-12-08
EP3305912A1 (en) 2018-04-11
PH12017502015A1 (en) 2018-04-02
US20210238653A1 (en) 2021-08-05
AU2016269875A2 (en) 2017-11-16
CN107849617B (zh) 2021-11-02
SG11201708920PA (en) 2017-11-29
JP6720161B2 (ja) 2020-07-08
AU2016269875A1 (en) 2017-11-02
WO2016194552A1 (ja) 2016-12-08
DK3305912T3 (da) 2021-12-06
TWI686481B (zh) 2020-03-01
US20180155764A1 (en) 2018-06-07
EP3305912A4 (en) 2018-04-11
CN107849617A (zh) 2018-03-27
TW201708545A (zh) 2017-03-01
KR102077577B1 (ko) 2020-02-17

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