BR112017026001A2 - kit para detectar conjuntamente, múltiplos ácidos nucleicos alvo que diferem entre si, e método de detecção usando o mesmo - Google Patents

kit para detectar conjuntamente, múltiplos ácidos nucleicos alvo que diferem entre si, e método de detecção usando o mesmo

Info

Publication number
BR112017026001A2
BR112017026001A2 BR112017026001-8A BR112017026001A BR112017026001A2 BR 112017026001 A2 BR112017026001 A2 BR 112017026001A2 BR 112017026001 A BR112017026001 A BR 112017026001A BR 112017026001 A2 BR112017026001 A2 BR 112017026001A2
Authority
BR
Brazil
Prior art keywords
nucleic acid
target nucleic
target
temperature
single strands
Prior art date
Application number
BR112017026001-8A
Other languages
English (en)
Japanese (ja)
Inventor
Nagano Takashi
MIYAJIMA Kensuke
Narahara Kenji
Original Assignee
Mizuho Medy Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mizuho Medy Co., Ltd. filed Critical Mizuho Medy Co., Ltd.
Publication of BR112017026001A2 publication Critical patent/BR112017026001A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

o documento apresenta um kit para detectar múltiplos ácidos nucleicos alvo capazes de amplificar e detectar múltiplos genes por meio de um recipiente de reação contendo um tipo de solução de reação e um tipo de rótulo. a solução pode conter primeiro ácido nucleico alvo (10) e o segundo ácido nucleico alvo (20), cada um dos quais se dissocia à temperatura de desnaturação t0. a solução contém ainda: dna polimerase (30); um iniciador de primeiro alvo (13) à temperatura de recombinação t1 ligando com os primeiros filamentos simples derivados do primeiro ácido nucleico alvo; um iniciador de segundo alvo (23) à temperatura de recombinação t1 ligando com os segundos filamentos simples derivados do segundo ácido nucleico alvo; uma sonda do primeiro alvo (15) à temperatura de recombinação t1 ligando com os primeiros filamentos simples derivados do primeiro ácido nucleico alvo; e uma sonda de segundo alvo (25) à temperatura de detecção do segundo alvo t3 que é inferior à temperatura de recombinação t1 e temperatura de alongamento t2 ligando com os segundos filamentos simples derivados do segundo ácido nucleico alvo. uma condição de que: t0 seja superior a t2; t2 não seja inferior a t1; e t1 seja superior a t3 foi satisfeita.
BR112017026001-8A 2015-06-04 2016-05-10 kit para detectar conjuntamente, múltiplos ácidos nucleicos alvo que diferem entre si, e método de detecção usando o mesmo BR112017026001A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2015-113979 2015-06-04
JP2015113979 2015-06-04
PCT/JP2016/063831 WO2016194552A1 (ja) 2015-06-04 2016-05-10 複数の標的核酸の検出キット及びそれを用いる検出方法

Publications (1)

Publication Number Publication Date
BR112017026001A2 true BR112017026001A2 (pt) 2018-11-06

Family

ID=57439990

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017026001-8A BR112017026001A2 (pt) 2015-06-04 2016-05-10 kit para detectar conjuntamente, múltiplos ácidos nucleicos alvo que diferem entre si, e método de detecção usando o mesmo

Country Status (16)

Country Link
US (2) US20180155764A1 (pt)
EP (1) EP3305912B1 (pt)
JP (1) JP6720161B2 (pt)
KR (1) KR102077577B1 (pt)
CN (1) CN107849617B (pt)
AU (1) AU2016269875B2 (pt)
BR (1) BR112017026001A2 (pt)
CA (1) CA2983428C (pt)
DK (1) DK3305912T3 (pt)
ES (1) ES2899826T3 (pt)
MX (1) MX2017015673A (pt)
MY (1) MY182821A (pt)
PH (1) PH12017502015A1 (pt)
SG (1) SG11201708920PA (pt)
TW (1) TWI686481B (pt)
WO (1) WO2016194552A1 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
US20210040542A1 (en) * 2018-04-20 2021-02-11 Seegene, Inc. Method And Apparatus For Detecting A Plurality Of Target Nucleic Acid Sequences In Sample
JP7221491B2 (ja) * 2018-09-18 2023-02-14 株式会社ミズホメディー 複数の標的核酸を検出するキットを用いる検出方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4724380Y1 (pt) 1969-11-20 1972-08-01
US6174670B1 (en) 1996-06-04 2001-01-16 University Of Utah Research Foundation Monitoring amplification of DNA during PCR
DE60140804D1 (de) * 2000-06-27 2010-01-28 Nat Inst Of Advanced Ind Scien Neue nukleinsäuresonden und verfahren zum testen von nukleinsäuren unter deren verwendung
JP2002136300A (ja) 2000-08-25 2002-05-14 Otsuka Pharmaceut Co Ltd 白血病キメラ遺伝子の検出方法
JP4460228B2 (ja) 2002-04-22 2010-05-12 日鉄環境エンジニアリング株式会社 核酸の新規測定方法
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
EP1411133B1 (en) * 2002-09-24 2009-01-07 Qiagen GmbH Enhanced coamplification of nucleic acids
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
EP2116614A1 (en) * 2008-05-06 2009-11-11 Qiagen GmbH Simultaneous detection of multiple nucleic acid sequences in a reaction
US20120100526A1 (en) * 2008-12-10 2012-04-26 Smiths Detection Inc. Identification and differentiation of nucleic acid sequence using temperature-dependent hybridization
EP2379747B1 (en) * 2008-12-22 2013-07-03 University of Utah Research Foundation Monochrome multiplex quantitative pcr
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
CN101624629B (zh) * 2009-07-24 2012-09-05 上海浩源生物科技有限公司 单管中多重靶核酸的pcr检测方法及其试剂盒
GB201100150D0 (en) * 2011-01-06 2011-02-23 Epistem Ltd Mutational analysis
JP5863162B2 (ja) * 2011-06-17 2016-02-16 広島県 標的核酸の検出方法
US10876160B2 (en) * 2011-10-31 2020-12-29 Eiken Kagaku Kabushiki Kaisha Method for detecting target nucleic acid

Also Published As

Publication number Publication date
WO2016194552A1 (ja) 2016-12-08
AU2016269875B2 (en) 2019-05-09
SG11201708920PA (en) 2017-11-29
PH12017502015A1 (en) 2018-04-02
US20210238653A1 (en) 2021-08-05
KR102077577B1 (ko) 2020-02-17
AU2016269875A1 (en) 2017-11-02
EP3305912B1 (en) 2021-10-06
EP3305912A1 (en) 2018-04-11
MX2017015673A (es) 2018-06-19
CN107849617B (zh) 2021-11-02
JP6720161B2 (ja) 2020-07-08
JPWO2016194552A1 (ja) 2018-03-22
DK3305912T3 (da) 2021-12-06
TWI686481B (zh) 2020-03-01
MY182821A (en) 2021-02-05
EP3305912A4 (en) 2018-04-11
CA2983428A1 (en) 2016-12-08
CA2983428C (en) 2021-12-07
ES2899826T3 (es) 2022-03-14
US20180155764A1 (en) 2018-06-07
AU2016269875A2 (en) 2017-11-16
TW201708545A (zh) 2017-03-01
KR20170139150A (ko) 2017-12-18
CN107849617A (zh) 2018-03-27

Similar Documents

Publication Publication Date Title
JP2016195614A5 (pt)
ES2610461T3 (es) Método de detección de nucleótidos únicos
WO2017065959A3 (en) Methods and compositions that utilize transcriptome sequencing data in machine learning-based classification
RU2017101174A (ru) Набор или устройство для обнаружения рака печени и способ обнаружения
ES2559108T3 (es) Método de detección de nucleótidos simples
BR112017020363A2 (pt) método para determinar a presença de uma variante em um ou mais genes em um indivíduo, sistema, e, meio legível por computador não transitório
RU2017101353A (ru) Набор или устройство для обнаружения рака пищевода и способ обнаружения
WO2015119941A3 (en) Genome fractioning
BR122022007092B8 (pt) Método para construir um perfil de dna, método para construir uma biblioteca de ácido nucléico, biblioteca de ácido nucléico, pluralidade de iniciadores e kit
EP4321627A3 (en) Spatially distinguished, multiplex nucleic acid analysis of biological specimens
WO2014116729A3 (en) Haplotying of hla loci with ultra-deep shotgun sequencing
WO2015200893A3 (en) Methods of analyzing nucleic acids from individual cells or cell populations
BR112017007912A2 (pt) transposon de preservação de contiguidade
BR112017008022A2 (pt) célula, polinucleotídeo, veículo, e, métodos para modificação de um genoma de células cho e para preparação de uma proteína de interesse.
RU2017100884A (ru) Набор или устройство для обнаружения рака ободочной и прямой кишки и способ обнаружения
BR112017008082A2 (pt) composições e métodos para detectar um vírus de rna
BR112017026001A2 (pt) kit para detectar conjuntamente, múltiplos ácidos nucleicos alvo que diferem entre si, e método de detecção usando o mesmo
CY1122788T1 (el) Ανιχνευση πυρηνικου οξεος στοχου και παραλλαγματων
GB2581599B8 (en) Tagging nucleic acid molecules from single cells for phased sequencing
EP4321867A3 (en) Probes for improved melt discrimination and multiplexing in nucleic acid assays
MX2018014163A (es) Composiciones de nucleotido marcados y metodos para el secuenciamiento de acido nucleico.
ES2614816T3 (es) Método para la detección de mutaciones KRAS
BR112017008702A2 (pt) ?mutante de endoxilanase, dna, vetor, célula, método de produção de mutantes de endoxilanase, composição enzimática e método de produção de solução de açúcar?
BR112018072729A2 (pt) sistema genético reverso do vírus da zica
CL2016000299A1 (es) Metodo para la deteccion de resistencia a piretroides en crustaceos usando secuencias oligonucleotidas utiles en la deteccion de resistencia a piretroide.

Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]