BR112017026001A2 - kit para detectar conjuntamente, múltiplos ácidos nucleicos alvo que diferem entre si, e método de detecção usando o mesmo - Google Patents

kit para detectar conjuntamente, múltiplos ácidos nucleicos alvo que diferem entre si, e método de detecção usando o mesmo

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Publication number
BR112017026001A2
BR112017026001A2 BR112017026001-8A BR112017026001A BR112017026001A2 BR 112017026001 A2 BR112017026001 A2 BR 112017026001A2 BR 112017026001 A BR112017026001 A BR 112017026001A BR 112017026001 A2 BR112017026001 A2 BR 112017026001A2
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BR
Brazil
Prior art keywords
nucleic acid
target nucleic
target
temperature
single strands
Prior art date
Application number
BR112017026001-8A
Other languages
English (en)
Japanese (ja)
Inventor
Nagano Takashi
MIYAJIMA Kensuke
Narahara Kenji
Original Assignee
Mizuho Medy Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mizuho Medy Co., Ltd. filed Critical Mizuho Medy Co., Ltd.
Publication of BR112017026001A2 publication Critical patent/BR112017026001A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

o documento apresenta um kit para detectar múltiplos ácidos nucleicos alvo capazes de amplificar e detectar múltiplos genes por meio de um recipiente de reação contendo um tipo de solução de reação e um tipo de rótulo. a solução pode conter primeiro ácido nucleico alvo (10) e o segundo ácido nucleico alvo (20), cada um dos quais se dissocia à temperatura de desnaturação t0. a solução contém ainda: dna polimerase (30); um iniciador de primeiro alvo (13) à temperatura de recombinação t1 ligando com os primeiros filamentos simples derivados do primeiro ácido nucleico alvo; um iniciador de segundo alvo (23) à temperatura de recombinação t1 ligando com os segundos filamentos simples derivados do segundo ácido nucleico alvo; uma sonda do primeiro alvo (15) à temperatura de recombinação t1 ligando com os primeiros filamentos simples derivados do primeiro ácido nucleico alvo; e uma sonda de segundo alvo (25) à temperatura de detecção do segundo alvo t3 que é inferior à temperatura de recombinação t1 e temperatura de alongamento t2 ligando com os segundos filamentos simples derivados do segundo ácido nucleico alvo. uma condição de que: t0 seja superior a t2; t2 não seja inferior a t1; e t1 seja superior a t3 foi satisfeita.
BR112017026001-8A 2015-06-04 2016-05-10 kit para detectar conjuntamente, múltiplos ácidos nucleicos alvo que diferem entre si, e método de detecção usando o mesmo BR112017026001A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2015-113979 2015-06-04
JP2015113979 2015-06-04
PCT/JP2016/063831 WO2016194552A1 (ja) 2015-06-04 2016-05-10 複数の標的核酸の検出キット及びそれを用いる検出方法

Publications (1)

Publication Number Publication Date
BR112017026001A2 true BR112017026001A2 (pt) 2018-11-06

Family

ID=57439990

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017026001-8A BR112017026001A2 (pt) 2015-06-04 2016-05-10 kit para detectar conjuntamente, múltiplos ácidos nucleicos alvo que diferem entre si, e método de detecção usando o mesmo

Country Status (16)

Country Link
US (2) US20180155764A1 (pt)
EP (1) EP3305912B1 (pt)
JP (1) JP6720161B2 (pt)
KR (1) KR102077577B1 (pt)
CN (1) CN107849617B (pt)
AU (1) AU2016269875B2 (pt)
BR (1) BR112017026001A2 (pt)
CA (1) CA2983428C (pt)
DK (1) DK3305912T3 (pt)
ES (1) ES2899826T3 (pt)
MX (1) MX2017015673A (pt)
MY (1) MY182821A (pt)
PH (1) PH12017502015A1 (pt)
SG (1) SG11201708920PA (pt)
TW (1) TWI686481B (pt)
WO (1) WO2016194552A1 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
AU2019255933B2 (en) * 2018-04-20 2022-08-25 Seegene, Inc. . Method and apparatus for detecting a plurality of target nucleic acid sequences in sample
JP7221491B2 (ja) * 2018-09-18 2023-02-14 株式会社ミズホメディー 複数の標的核酸を検出するキットを用いる検出方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4724380Y1 (pt) 1969-11-20 1972-08-01
CA2257109C (en) 1996-06-04 2009-10-06 University Of Utah Research Foundation Monitoring hybridization during pcr
WO2002008414A1 (fr) * 2000-06-27 2002-01-31 National Institute Of Advanced Industrial Science And Technology Nouvelles sondes d'acides nucléiques et procédés d'essai d'acide nucléique par utilisation des mêmes
JP2002136300A (ja) 2000-08-25 2002-05-14 Otsuka Pharmaceut Co Ltd 白血病キメラ遺伝子の検出方法
JP4460228B2 (ja) 2002-04-22 2010-05-12 日鉄環境エンジニアリング株式会社 核酸の新規測定方法
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
EP1411133B1 (en) * 2002-09-24 2009-01-07 Qiagen GmbH Enhanced coamplification of nucleic acids
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
EP2336358A1 (en) * 2008-05-06 2011-06-22 QIAGEN GmbH Simultaneous detection of multiple nucleic acid sequences in a reaction
CN102301007A (zh) * 2008-12-10 2011-12-28 史密斯探测公司 使用随温度而变的杂交对核酸序列进行鉴定与区分
JP5840950B2 (ja) * 2008-12-22 2016-01-06 ユニバーシティ・オブ・ユタ・リサーチ・ファウンデイション 単色マルチプレックス定量ｐｃｒ
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
CN101624629B (zh) * 2009-07-24 2012-09-05 上海浩源生物科技有限公司 单管中多重靶核酸的pcr检测方法及其试剂盒
GB201100150D0 (en) * 2011-01-06 2011-02-23 Epistem Ltd Mutational analysis
JP5863162B2 (ja) * 2011-06-17 2016-02-16 広島県 標的核酸の検出方法
ES2755934T3 (es) * 2011-10-31 2020-04-24 Eiken Chemical Método para detectar ácido nucleico diana

Also Published As

Publication number Publication date
EP3305912B1 (en) 2021-10-06
TWI686481B (zh) 2020-03-01
SG11201708920PA (en) 2017-11-29
MX2017015673A (es) 2018-06-19
EP3305912A4 (en) 2018-04-11
TW201708545A (zh) 2017-03-01
KR102077577B1 (ko) 2020-02-17
PH12017502015A1 (en) 2018-04-02
US20210238653A1 (en) 2021-08-05
EP3305912A1 (en) 2018-04-11
AU2016269875A2 (en) 2017-11-16
CA2983428C (en) 2021-12-07
WO2016194552A1 (ja) 2016-12-08
US20180155764A1 (en) 2018-06-07
AU2016269875B2 (en) 2019-05-09
DK3305912T3 (da) 2021-12-06
AU2016269875A1 (en) 2017-11-02
ES2899826T3 (es) 2022-03-14
CN107849617B (zh) 2021-11-02
KR20170139150A (ko) 2017-12-18
MY182821A (en) 2021-02-05
CN107849617A (zh) 2018-03-27
CA2983428A1 (en) 2016-12-08
JP6720161B2 (ja) 2020-07-08
JPWO2016194552A1 (ja) 2018-03-22

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]