BR112017008702A2 - ?mutante de endoxilanase, dna, vetor, célula, método de produção de mutantes de endoxilanase, composição enzimática e método de produção de solução de açúcar? - Google Patents

?mutante de endoxilanase, dna, vetor, célula, método de produção de mutantes de endoxilanase, composição enzimática e método de produção de solução de açúcar?

Info

Publication number
BR112017008702A2
BR112017008702A2 BR112017008702-2A BR112017008702A BR112017008702A2 BR 112017008702 A2 BR112017008702 A2 BR 112017008702A2 BR 112017008702 A BR112017008702 A BR 112017008702A BR 112017008702 A2 BR112017008702 A2 BR 112017008702A2
Authority
BR
Brazil
Prior art keywords
production method
endoxylanase mutant
endoxylanase
dna
vector
Prior art date
Application number
BR112017008702-2A
Other languages
English (en)
Japanese (ja)
Inventor
Kurihara Hiroyuki
Kasahara Takuya
Yamada Chiaki
Murakami Natsuko
Yamada Katsushige
Ishikawa Kazuhiko
Watanabe Masahiro
Original Assignee
Toray Industries, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toray Industries, Inc. filed Critical Toray Industries, Inc.
Publication of BR112017008702A2 publication Critical patent/BR112017008702A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

é fornecido um mutante de endoxilanase que possui estabilidade térmica aprimorada. mutante de endoxilanase que possui atividade de endoxilanase e compreende uma sequência de aminoácidos que inclui pelo menos a substituição de um resíduo de aminoácido em uma ou mais posições selecionadas a partir de posições correspondentes à posição 35, posição 44, posição 62, posição 63, posição 101 e posição 102 de uma sequência de aminoácidos de seq id nº 1 em uma sequência de aminoácidos de endoxilanase derivada de fungo filamentoso.
BR112017008702-2A 2014-11-05 2015-11-05 ?mutante de endoxilanase, dna, vetor, célula, método de produção de mutantes de endoxilanase, composição enzimática e método de produção de solução de açúcar? BR112017008702A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2014-225543 2014-11-05
JP2014225543 2014-11-05
PCT/JP2015/081151 WO2016072448A1 (ja) 2014-11-05 2015-11-05 エンドキシラナーゼ変異体、バイオマス分解用酵素組成物及び糖液の製造方法

Publications (1)

Publication Number Publication Date
BR112017008702A2 true BR112017008702A2 (pt) 2018-02-27

Family

ID=55909172

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017008702-2A BR112017008702A2 (pt) 2014-11-05 2015-11-05 ?mutante de endoxilanase, dna, vetor, célula, método de produção de mutantes de endoxilanase, composição enzimática e método de produção de solução de açúcar?

Country Status (7)

Country Link
US (1) US10435728B2 (pt)
EP (1) EP3216864A4 (pt)
JP (1) JP6689487B2 (pt)
AU (1) AU2015344324B2 (pt)
BR (1) BR112017008702A2 (pt)
CA (1) CA2966330A1 (pt)
WO (1) WO2016072448A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7046370B2 (ja) * 2017-02-23 2022-04-04 国立研究開発法人産業技術総合研究所 キシラナーゼ変異体、及びバイオマス分解用酵素組成物
CN110904078B (zh) * 2019-12-11 2020-09-04 云南师范大学 一种耐硫酸钠和硫酸铵的木糖苷酶突变体v322r及其应用
CN111004789B (zh) * 2019-12-11 2021-10-15 云南师范大学 一种耐硫酸铵的木糖苷酶突变体v322dh328dt329e
CN111073876B (zh) * 2020-01-18 2021-07-27 江南大学 热稳定性提高的枯草芽孢杆菌脂肪酶a

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0976838A1 (en) 1998-05-06 2000-02-02 Rhone-Poulenc Nutrition Animale Enzymes mixture
FI108728B (fi) 1999-10-12 2002-03-15 Carbozyme Oy Menetelmä perheen G/11 ksylanaasien stabiilisuuden parantamiseksi ja optimaalisen pH-alueen muuttamiseksi
JP2002199890A (ja) 2000-10-23 2002-07-16 Inst Of Physical & Chemical Res 生分解性ポリエステル合成酵素の改変方法
JP4117350B2 (ja) * 2005-03-03 2008-07-16 富山県 機能改変フェニルアラニン脱水素酵素、およびこの酵素を用いた生体試料中のアミノ酸の分析方法
JP5622321B2 (ja) 2008-06-19 2014-11-12 日本化薬株式会社 耐熱性１，５−アンヒドログルシトール脱水素酵素及びそれを用いた１，５−アンヒドログルシトールの測定法
DK3037528T3 (en) * 2008-12-23 2018-12-10 Dupont Nutrition Biosci Aps POLYPEPTIDES
WO2010072226A1 (en) * 2008-12-23 2010-07-01 Danisco A/S Polypeptides with xylanase activity
WO2013103127A1 (ja) 2012-01-06 2013-07-11 本田技研工業株式会社 糖化酵素組成物及びそれを用いる糖化溶液の製造方法
JP2013243954A (ja) 2012-05-24 2013-12-09 Kao Corp キシラナーゼおよびそれを用いた糖の製造方法
JP2014064563A (ja) 2012-09-04 2014-04-17 Toray Ind Inc 糖液の製造方法
JP6056870B2 (ja) * 2012-10-19 2017-01-11 国立研究開発法人産業技術総合研究所 新規キシラナーゼ
CN103642777A (zh) 2013-12-10 2014-03-19 江南大学 一种提高米曲霉木聚糖酶热稳定性的方法

Also Published As

Publication number Publication date
JPWO2016072448A1 (ja) 2017-08-17
EP3216864A4 (en) 2018-05-02
US10435728B2 (en) 2019-10-08
EP3216864A1 (en) 2017-09-13
JP6689487B2 (ja) 2020-04-28
US20170240941A1 (en) 2017-08-24
AU2015344324B2 (en) 2021-09-30
AU2015344324A2 (en) 2017-06-08
WO2016072448A1 (ja) 2016-05-12
CA2966330A1 (en) 2016-05-12
AU2015344324A8 (en) 2017-05-11
AU2015344324A1 (en) 2017-04-27

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 7A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2695 DE 30-08-2022 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.