ES2559108T3 - Método de detección de nucleótidos simples - Google Patents

Método de detección de nucleótidos simples Download PDF

Info

Publication number
ES2559108T3
ES2559108T3 ES14718148.1T ES14718148T ES2559108T3 ES 2559108 T3 ES2559108 T3 ES 2559108T3 ES 14718148 T ES14718148 T ES 14718148T ES 2559108 T3 ES2559108 T3 ES 2559108T3
Authority
ES
Spain
Prior art keywords
nucleotide
oligonucleotide
single stranded
monobasic
region
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES14718148.1T
Other languages
English (en)
Inventor
Cameron Alexander FRAYLING
Barnaby BALMFORTH
Bruno Flavio Nogueira de Sousa SOARES
Thomas Henry ISAAC
Boris Breiner
Alessandra NATALE
Michele AMASIO
Paul Dear
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medical Research Council
Base4 Innovation Ltd
Original Assignee
Medical Research Council
Base4 Innovation Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Research Council, Base4 Innovation Ltd filed Critical Medical Research Council
Application granted granted Critical
Publication of ES2559108T3 publication Critical patent/ES2559108T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Fodder In General (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Un método para determinación de la secuencia de bases nucleotídicas en un analito polinucleotídico caracterizado por los pasos de (1) generar una corriente de trifosfatos nucleotídicos monobásicos a partir del analito por pirofosforólisis progresiva en presencia de una enzima de pirofosforólisis; (2) producir moléculas capturadas por reacción de cada trifosfato nucleotídico monobásico en presencia de una polimerasa y una ligasa con un sistema de captura constituido por: (i) (a) un primer oligonucleótido que comprende una región bicatenaria y una región monocatenaria y (b) un segundo oligonucleótido monocatenario cuya secuencia de bases nucleotídicas es al menos parcialmente complementaria a la de la región monocatenaria del primer oligonucleótido; o (ii) un oligonucleótido simple que comprende una región nucleotídica monocatenaria cuyos extremos están unidos a dos regiones oligonucleotídicas bicatenarias diferentes; (3) amplificar al menos parte de la molécula capturada para producir una pluralidad de amplicones característicos del trifosfato nucleotídico monobásico simple, (4) marcar los amplicones con una sonda correspondiente que tiene un elemento característico detectable y (5) detectar una propiedad característica del elemento detectable.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
imagen6

Claims (1)

  1. imagen1
    imagen2
ES14718148.1T 2013-04-09 2014-04-09 Método de detección de nucleótidos simples Active ES2559108T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1306445.6A GB201306445D0 (en) 2013-04-09 2013-04-09 Single nucleotide detection method
GB201306445 2013-04-09
PCT/GB2014/051106 WO2014167324A1 (en) 2013-04-09 2014-04-09 Single nucleotide detection method

Publications (1)

Publication Number Publication Date
ES2559108T3 true ES2559108T3 (es) 2016-02-10

Family

ID=48483634

Family Applications (1)

Application Number Title Priority Date Filing Date
ES14718148.1T Active ES2559108T3 (es) 2013-04-09 2014-04-09 Método de detección de nucleótidos simples

Country Status (12)

Country Link
US (1) US10480024B2 (es)
EP (1) EP2828409B1 (es)
JP (1) JP6289610B2 (es)
KR (1) KR101766657B1 (es)
CN (1) CN105121662B (es)
AU (1) AU2014252805B2 (es)
BR (1) BR112015025763A2 (es)
CA (1) CA2907868C (es)
DK (1) DK2828409T3 (es)
ES (1) ES2559108T3 (es)
GB (1) GB201306445D0 (es)
WO (1) WO2014167324A1 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9974611B2 (en) 2012-12-06 2018-05-22 Exomed International Ip, Llc Ergonomic surgical glove
US10130438B2 (en) 2013-01-30 2018-11-20 Exsomed International IP, LLC Surgical glove with ergonomic features
US9622523B2 (en) 2014-01-06 2017-04-18 Exsomed International IP, LLC Ergonomic work gloves
GB201402644D0 (en) * 2014-02-14 2014-04-02 Base4 Innovation Ltd Methylation detection method
GB201412977D0 (en) 2014-07-22 2014-09-03 Base4 Innovation Ltd Single nucleotide detection method
GB201501012D0 (en) * 2015-01-21 2015-03-04 Base4 Innovation Ltd Improved droplet sequencing apparatus and method
EP3207982A1 (en) 2016-02-17 2017-08-23 Base4 Innovation Limited Improved droplet sequencing apparatus and method
EP3211092B1 (en) 2016-02-24 2019-03-27 Base4 Innovation Ltd Single nucleotide detection method
US20190226014A1 (en) 2016-09-02 2019-07-25 Base4 Innovation Limited Single nucleotide detection method and associated probes
JP2019531069A (ja) 2016-09-06 2019-10-31 ベース４ イノベーション リミテッド 単一ヌクレオチド検出方法および関連プローブ
WO2018054964A1 (en) 2016-09-20 2018-03-29 Base4 Innovation Limited Single nucleotide detection method and associated probes
WO2018210823A1 (en) 2017-05-15 2018-11-22 Base4 Innovation Limited Single nucleotide detection method and associated probes
BR112019027764A2 (pt) 2017-06-21 2020-07-07 Base4 Innovation Limited método para manipular uma microgotícula e método para pirofosforizar um analito de ácido nucleico
IL271545B2 (en) 2017-06-21 2023-09-01 Base4 Innovation Ltd Microfluidic analytical device
WO2019243577A1 (en) 2018-06-21 2019-12-26 Base4 Innovation Limited Sequencing method using modified nucleoside polyphosphates

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2575270B2 (ja) 1992-11-10 1997-01-22 浜松ホトニクス株式会社 核酸の塩基配列決定方法、単一分子検出方法、その装置及び試料の作成方法
CA2155186A1 (en) 1993-02-01 1994-08-18 Kevin M. Ulmer Methods and apparatus for dna sequencing
US6268146B1 (en) * 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
WO2000071755A2 (en) * 1999-05-25 2000-11-30 Praelux Incorporated Method for sequency and characterizing polymeric biomolecules using aptamers and a method for producing aptamers
EP1448798A1 (de) * 2001-11-27 2004-08-25 Gnothis Holding SA Nanostruktur insbesondere zur analyse von einzelmolekülen
WO2003080861A1 (en) * 2002-03-22 2003-10-02 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Single molecule sequencing using phosphate labeled nucleotides
US7476501B2 (en) 2002-03-26 2009-01-13 Intel Corporation Methods and device for DNA sequencing using surface enhanced raman scattering (SERS)
US9388459B2 (en) 2002-06-17 2016-07-12 Affymetrix, Inc. Methods for genotyping
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
US20070015176A1 (en) * 2005-02-18 2007-01-18 Applera Corporation Small nucleic acid detection probes and uses thereof
EP2016189B1 (en) * 2006-04-18 2012-01-25 Advanced Liquid Logic, Inc. Droplet-based pyrosequencing
US8389297B2 (en) * 2006-04-18 2013-03-05 Duke University Droplet-based affinity assay device and system
US7439014B2 (en) * 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8815576B2 (en) 2007-12-27 2014-08-26 Lawrence Livermore National Security, Llc. Chip-based sequencing nucleic acids
US20090246788A1 (en) 2008-04-01 2009-10-01 Roche Nimblegen, Inc. Methods and Assays for Capture of Nucleic Acids
US20110311980A1 (en) 2008-12-15 2011-12-22 Advanced Liquid Logic, Inc. Nucleic Acid Amplification and Sequencing on a Droplet Actuator
US20120164633A1 (en) 2010-12-27 2012-06-28 Ibis Biosciences, Inc. Digital droplet sequencing

Also Published As

Publication number Publication date
JP6289610B2 (ja) 2018-03-07
CN105121662B (zh) 2017-03-22
US20160040223A1 (en) 2016-02-11
CN105121662A (zh) 2015-12-02
EP2828409B1 (en) 2015-10-14
US10480024B2 (en) 2019-11-19
BR112015025763A2 (pt) 2017-10-17
AU2014252805B2 (en) 2017-01-05
DK2828409T3 (en) 2015-12-07
GB201306445D0 (en) 2013-05-22
WO2014167324A1 (en) 2014-10-16
JP2016519576A (ja) 2016-07-07
KR20150143614A (ko) 2015-12-23
CA2907868A1 (en) 2014-10-16
CA2907868C (en) 2017-08-22
AU2014252805A1 (en) 2015-10-15
EP2828409A1 (en) 2015-01-28
KR101766657B1 (ko) 2017-08-23

Similar Documents

Publication Publication Date Title
ES2559108T3 (es) Método de detección de nucleótidos simples
ES2610461T3 (es) Método de detección de nucleótidos únicos
RU2017125417A (ru) Обнаружение изменений конформации полимеразы нуклеиновых кислот с помощью нанотрубки
BR112017008082A2 (pt) composições e métodos para detectar um vírus de rna
WO2015123430A3 (en) Single molecule electronic multiplex snp assay and pcr analysis
BR112015025762A2 (pt) método e dispositivo para determinar a sequência de bases de nucleotídeos em um analito de polinucleotídeos
BR112016019267A2 (pt) métodos e composições para perfil de dna
JP2015156872A5 (es)
BR112012019396A2 (pt) método e kit para amplificar um ácido nucleico.
WO2017065959A3 (en) Methods and compositions that utilize transcriptome sequencing data in machine learning-based classification
MY157586A (en) Method for detecting target nucleic acid
BR112017024702A2 (pt) detecção de ácidos nucleicos-alvo e variantes
WO2016059473A3 (en) Sequence conversion and signal amplifier dna having locked nucleic acids and detection methods using same
CO2018011856A2 (es) Composiciones, métodos y kits para la síntesis y detección de ácidos nucleicos
JP2014030431A5 (es)
JP2016512437A5 (es)
WO2018089945A8 (en) Polynucleotides for the amplification and detection of neisseria gonorrhoeae
BR112014014307A2 (pt) métodos e kits para detecção in situ à temperatura ambiente de um ácido nucleico alvo em uma amostra biológica
CL2017001929A1 (es) Método y producto
CO2022001223A2 (es) Polinucleótidos para la amplificación y detección de neisseria gonorrhoeae
EA201692118A1 (ru) Зонд на основе нуклеиновой кислоты, способ конструирования зонда на основе нуклеиновой кислоты и способ обнаружения последовательности-мишени
ES2640570T3 (es) Ensayo de doble sonda para la detección de VHC
WO2016073255A8 (en) Detection of hiv-1 nucleic acids by reverse-transcription loop-mediated isothermal amplication
EA201491821A1 (ru) Модифицированные олигонуклеотиды, включающие тиольные функциональные группы, и их применение для детекции нуклеиновых кислот
BR112018011141A2 (pt) método para detectar o gênero fetal e as anormalidades no número de cópias, aparelho e meio legível por computador para realizar o mesmo