WO2012121486A3 - Sdl-pcr을 이용한 유전자 분석방법 - Google Patents

Sdl-pcr을 이용한 유전자 분석방법 Download PDF

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Publication number
WO2012121486A3
WO2012121486A3 PCT/KR2012/000695 KR2012000695W WO2012121486A3 WO 2012121486 A3 WO2012121486 A3 WO 2012121486A3 KR 2012000695 W KR2012000695 W KR 2012000695W WO 2012121486 A3 WO2012121486 A3 WO 2012121486A3
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Prior art keywords
pcr
sdl
ligation
probe
subjected
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PCT/KR2012/000695
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English (en)
French (fr)
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WO2012121486A2 (ko
WO2012121486A9 (ko
Inventor
박현규
신성철
김가희
강병철
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한국과학기술원
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Priority to US14/002,662 priority Critical patent/US9523117B2/en
Publication of WO2012121486A2 publication Critical patent/WO2012121486A2/ko
Publication of WO2012121486A9 publication Critical patent/WO2012121486A9/ko
Publication of WO2012121486A3 publication Critical patent/WO2012121486A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 SDL-PCR(Separation of displaced ligation probe-based PCR)을 이용한 유전자 분석방법에 관한 것으로, 보다 구체적으로, 목적 유전자와 상보적인 염기서열을 포함하는 각각의 탐침들을 리가제(ligase)를 이용하여 연결반응시키고, 상기 탐침과 혼성화될 수 있는 또 다른 탐침을 첨가하여 혼성화시킨다음 연장(extention)반응시켜 주형 탐침을 제조하고, 공통의 프라이머를 이용하여 목적유전자의 주형 탐침을 증폭시키는 것을 특징으로 하는 SDL-PCR을 이용한 유전자 분석방법에 관한 것이다. 본 발명에 따른 SDL-PCR 방법은 연결 반응이 되지 않은 미반응 탐침 또는 genomic DNA를 태그를 이용하여 제거함으로써, 비특이적 증폭 반응을 최소화할 수 있을 뿐만 아니라 exonuclease를 이용하는 정제방법보다 빠른 시간 내에 정제가 가능하며 단일 튜브 내의 동일 용액에서 연결, 정제 및 중합효소연쇄반응이 가능하여 정확하고 신속하게 복수의 유전자를 동시에 증폭시킬 수 있다.
PCT/KR2012/000695 2011-03-04 2012-01-30 Sdl-pcr을 이용한 유전자 분석방법 WO2012121486A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US14/002,662 US9523117B2 (en) 2011-03-04 2012-01-30 Gene analysis method using SDL-PCR

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0019225 2011-03-04
KR1020110019225A KR101322880B1 (ko) 2011-03-04 2011-03-04 Ｓｄｌ－ｐｃｒ을 이용한 유전자 분석방법

Publications (3)

Publication Number Publication Date
WO2012121486A2 WO2012121486A2 (ko) 2012-09-13
WO2012121486A9 WO2012121486A9 (ko) 2012-10-18
WO2012121486A3 true WO2012121486A3 (ko) 2012-12-20

Family

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Country Link
US (1) US9523117B2 (ko)
KR (1) KR101322880B1 (ko)
WO (1) WO2012121486A2 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG194745A1 (en) * 2011-05-20 2013-12-30 Fluidigm Corp Nucleic acid encoding reactions
CN105803055A (zh) * 2014-12-31 2016-07-27 天昊生物医药科技（苏州）有限公司 一种基于多重循环延伸连接的靶基因区域富集新方法
US11117113B2 (en) 2015-12-16 2021-09-14 Fluidigm Corporation High-level multiplex amplification

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030082584A1 (en) * 2001-06-29 2003-05-01 Liang Shi Enzymatic ligation-based identification of transcript expression
WO2009140802A1 (zh) * 2008-05-23 2009-11-26 陕西北美基因股份有限公司 检测探针、通用寡核苷酸芯片及核酸检测方法及其用途
US20100267585A1 (en) * 2009-04-01 2010-10-21 Dxterity Diagnostics Chemical ligation dependent probe amplification (CLPA)

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EP1130113A1 (en) * 2000-02-15 2001-09-05 Johannes Petrus Schouten Multiplex ligation dependent amplification assay
WO2003060163A2 (en) * 2001-12-28 2003-07-24 Keygene N.V. Discrimination and detection of target nucleotide sequences using mass spectrometry
EP1664345B1 (en) * 2003-09-02 2015-04-01 Keygene N.V. Ola-based methods for the detection of target nucleic acid sequences
US20050266417A1 (en) * 2003-09-12 2005-12-01 Francis Barany Methods for identifying target nucleic acid molecules
US8580494B2 (en) * 2006-08-25 2013-11-12 Research Foundation For Mental Hygiene, Inc. Methods and compositions for amplification and detection of MicroRNAs
WO2008069906A2 (en) * 2006-11-14 2008-06-12 The Regents Of The University Of California Digital expression of gene analysis
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Patent Citations (3)

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US20030082584A1 (en) * 2001-06-29 2003-05-01 Liang Shi Enzymatic ligation-based identification of transcript expression
WO2009140802A1 (zh) * 2008-05-23 2009-11-26 陕西北美基因股份有限公司 检测探针、通用寡核苷酸芯片及核酸检测方法及其用途
US20100267585A1 (en) * 2009-04-01 2010-10-21 Dxterity Diagnostics Chemical ligation dependent probe amplification (CLPA)

Non-Patent Citations (2)

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CARNEVALE, E. P. ET AL.: "A multiplex ligase detection reaction-fluorescent microsphere assay for simultaneous detection of single nucleotide polymorphisms associated with Plasmodium falciparum drug resistance", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 45, no. 3, March 2007 (2007-03-01), pages 752 - 761 *

Also Published As

Publication number Publication date
KR101322880B1 (ko) 2013-10-29
WO2012121486A2 (ko) 2012-09-13
US9523117B2 (en) 2016-12-20
US20140171334A1 (en) 2014-06-19
WO2012121486A9 (ko) 2012-10-18
KR20120101255A (ko) 2012-09-13

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