HRP20161056T1 - Metode za odabir i umnožavanje polinukleotida - Google Patents
Metode za odabir i umnožavanje polinukleotida Download PDFInfo
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- HRP20161056T1 HRP20161056T1 HRP20161056TT HRP20161056T HRP20161056T1 HR P20161056 T1 HRP20161056 T1 HR P20161056T1 HR P20161056T T HRP20161056T T HR P20161056TT HR P20161056 T HRP20161056 T HR P20161056T HR P20161056 T1 HRP20161056 T1 HR P20161056T1
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- amplification
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- 108091033319 polynucleotide Proteins 0.000 title claims 13
- 102000040430 polynucleotide Human genes 0.000 title claims 13
- 239000002157 polynucleotide Substances 0.000 title claims 13
- 238000000034 method Methods 0.000 title claims 12
- 230000003321 amplification Effects 0.000 claims 15
- 238000003199 nucleic acid amplification method Methods 0.000 claims 15
- 108091034117 Oligonucleotide Proteins 0.000 claims 14
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 12
- 230000000295 complement effect Effects 0.000 claims 6
- 239000002773 nucleotide Substances 0.000 claims 6
- 125000003729 nucleotide group Chemical group 0.000 claims 6
- 239000007787 solid Substances 0.000 claims 4
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims 3
- 239000002751 oligonucleotide probe Substances 0.000 claims 3
- 239000007790 solid phase Substances 0.000 claims 3
- 239000000758 substrate Substances 0.000 claims 3
- 239000013626 chemical specie Substances 0.000 claims 2
- 238000012163 sequencing technique Methods 0.000 claims 2
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims 1
- 238000000137 annealing Methods 0.000 claims 1
- 230000000903 blocking effect Effects 0.000 claims 1
- 238000009396 hybridization Methods 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 1
- 230000002441 reversible effect Effects 0.000 claims 1
- 239000000523 sample Substances 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Saccharide Compounds (AREA)
Claims (11)
1. Metoda za odabir i umnožavanje polinukleotida na čvrstoj podlozi, obuhvaća:
a) omogućavanje mnoštva oligonukleotida za umnožavanje imobiliziranih na čvrstoj podlozi;
b) hibridiziranje populacije oligonukleotidnih sondi na podskup navedenih oligonukleotida za umnožavanje, svaka od navedenih oligonukleotidnih sondi sadrži prvi dio koji je komplementaran oligonukleotidima za umnožavanje i drugi dio koji sadržislijed iz odabranog područja polinukleotidnog kalupa;
c) izvođenje reakcije produljivanja zbog produljivanja hibridiziranih oligonukleotida za umnožavanje u svrhu stvaranja populacije potporno učvršćenih veznih nukleotida, svaki vezni oligonukleotid u navedenoj populaciji sadrži slijed koji je komplementaran odabranom području polinukleotidnog kalupa, stoga omogućuje čvrstu podlogu koja obuhvaća populaciju potporno učvršćenih veznih nukleotida i populaciju neproduljenih oligonukleotida za umnožavanje;
d) primjenu populacije polinukleotida kalupa na čvrstu podlogu pod uvjetima selektivne hibridizacije polinukleotida kalupa na potporno učvršćene vezne nukleotide, pri čemu polinukleotidi kalupa sadrže adapterski slijed na 5' kraju, pri čemu je adapterski slijed isti kao slijed neproduljenih oligonukleotida za umnožavanje;
e) produljivanje potporno učvršćenih veznih nukleotida koji su hibridizirani za polinukleotide kalupa, stoga stvaraju produljene produkte, produljeni produkti imaju dio komplementaran polinukleotidima kalupa i dio komplementaran s neproduljenim oligonukleotidima za umnožavanje; i
f) umnožavanje produljenih produkata, pri čemu umnožavanje obuhvaća nalijeganje jednog ili više neproduljenih oligonukleotida za umnožavanje na jedan ili više produljenih produkata, stoga stvarajući produkt umnožavanja na čvrstoj fazi.
2. Metoda iz patentnog zahtjeva 1, nadalje obuhvaća:
g) sekvenciranje prvog lanca produkta umnožavanja na čvrstoj fazi iz koraka f) zbog dobivanja barem dijela slijeda nukleotida polinukleotida kalupa.
3. Metoda iz patentnog zahtjeva 2, pri čemu produkti umnožavanja na čvrstoj fazi obuhvaćaju vezno mjesto za univerzalnu početnicu za sekvenciranje.
4. Metoda iz patentnog zahtjeva 1, pri čemu vezni oligonukleotidi sadrže najmanje 10 različitih veznih slijedova.
5. Metoda iz patentnog zahtjeva 1, pri čemu su oligonukleotidi za umnožavanje, koji nisu hibridizirani s oligonukleotidnom sondom u koraku b) patentnog zahtjeva 1, reverzibilno blokirani za vrijeme produljivanja veznih nukleotida.
6. Metoda iz patentnog zahtjeva 5, pri čemu je reverzibilno blokiranje pomoću kemijskih vrsta pričvršćenih na 3' kraju oligonukleotida za umnožavanje.
7. Metoda iz patentnog zahtjeva 6, pri čemu je kemijska vrsta fosfatna grupa.
8. Metoda iz patentnog zahtjeva 1, pri čemu je umnožavanje izotermalno.
9. Metoda iz patentnog zahtjeva 1, pri čemu dio produljenih produkata komplementaran s neproduljenim oligonukleotidima za umnožavanje obuhvaća adapterski slijed.
10. Metoda iz patentnog zahtjeva 9, pri čemu polinukleotidi kalupa u uzorku nukleinske kiseline obuhvaćaju različite sljedove i adapterski slijed je isti za svaki polinukleotid kalupa.
11. Metoda iz patentnog zahtjeva 10, pri čemu svako mnoštvo oligonukleotida za umnožavanje obuhvaća zajednički slijed koji je komplementaran adapterskom slijedu koji je isti za svaki polinukleotidni kalup.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13180255.5A EP2669387B1 (en) | 2009-08-25 | 2009-08-25 | Methods for selecting and amplifying polynucleotides |
| PCT/US2009/054945 WO2011025477A1 (en) | 2009-08-25 | 2009-08-25 | Methods for selecting and amplifying polynucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20161056T1 true HRP20161056T1 (hr) | 2016-12-16 |
Family
ID=43628274
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20161056TT HRP20161056T1 (hr) | 2009-08-25 | 2016-08-22 | Metode za odabir i umnožavanje polinukleotida |
Country Status (15)
| Country | Link |
|---|---|
| US (4) | US8999642B2 (hr) |
| EP (3) | EP2470669B1 (hr) |
| CN (1) | CN102597256B (hr) |
| CA (1) | CA2770389C (hr) |
| CY (1) | CY1117970T1 (hr) |
| DK (2) | DK3133169T3 (hr) |
| ES (2) | ES2595055T3 (hr) |
| HR (1) | HRP20161056T1 (hr) |
| HU (1) | HUE030765T2 (hr) |
| LT (1) | LT2669387T (hr) |
| PL (1) | PL2669387T3 (hr) |
| PT (1) | PT2669387T (hr) |
| SI (1) | SI2669387T1 (hr) |
| SM (1) | SMT201600319B (hr) |
| WO (1) | WO2011025477A1 (hr) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010028288A2 (en) | 2008-09-05 | 2010-03-11 | Aueon, Inc. | Methods for stratifying and annotating cancer drug treatment options |
| WO2010038042A1 (en) * | 2008-10-02 | 2010-04-08 | Illumina Cambridge Ltd. | Nucleic acid sample enrichment for sequencing applications |
| US9029103B2 (en) | 2010-08-27 | 2015-05-12 | Illumina Cambridge Limited | Methods for sequencing polynucleotides |
| MX346956B (es) | 2010-09-24 | 2017-04-06 | Univ Leland Stanford Junior | Captura directa, amplificación y secuenciación de objetivo adn usando cebadores inmovilizados. |
| US9476095B2 (en) | 2011-04-15 | 2016-10-25 | The Johns Hopkins University | Safe sequencing system |
| EP3447495B2 (en) | 2012-10-29 | 2024-03-13 | The Johns Hopkins University | Papanicolaou test for ovarian and endometrial cancers |
| JP6410726B2 (ja) | 2012-12-10 | 2018-10-24 | レゾリューション バイオサイエンス， インコーポレイテッド | 標的化ゲノム解析のための方法 |
| EP3077539B8 (en) | 2013-12-02 | 2018-10-10 | Personal Genome Diagnostics Inc. | Method for evaluating minority variants in a sample |
| CA2931533C (en) | 2013-12-09 | 2023-08-08 | Illumina, Inc. | Methods and compositions for targeted nucleic acid sequencing |
| US10537889B2 (en) * | 2013-12-31 | 2020-01-21 | Illumina, Inc. | Addressable flow cell using patterned electrodes |
| US9677132B2 (en) | 2014-01-16 | 2017-06-13 | Illumina, Inc. | Polynucleotide modification on solid support |
| US9944924B2 (en) | 2014-01-16 | 2018-04-17 | Illumina, Inc. | Polynucleotide modification on solid support |
| EP3094742A1 (en) | 2014-01-16 | 2016-11-23 | Illumina, Inc. | Amplicon preparation and sequencing on solid supports |
| US10570447B2 (en) | 2014-05-16 | 2020-02-25 | Illumina, Inc. | Nucleic acid synthesis techniques |
| US20150353989A1 (en) | 2014-06-09 | 2015-12-10 | Illumina Cambridge Limited | Sample preparation for nucleic acid amplification |
| GB201410646D0 (en) * | 2014-06-14 | 2014-07-30 | Illumina Cambridge Ltd | Methods of increasing sequencing accuracy |
| MY182476A (en) | 2014-11-11 | 2021-01-25 | Illumina Cambridge Ltd | Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid |
| CN106148482B (zh) * | 2015-03-24 | 2019-12-03 | 深圳华大智造科技有限公司 | 一种适用于小型测序仪的测序方法 |
| CN116064738A (zh) * | 2015-07-27 | 2023-05-05 | 亿明达股份有限公司 | 核酸序列信息的空间定位 |
| US11286531B2 (en) | 2015-08-11 | 2022-03-29 | The Johns Hopkins University | Assaying ovarian cyst fluid |
| RU2018121254A (ru) | 2015-11-11 | 2019-12-16 | Резолюшн Байосайенс, Инк. | Высокоэффективное построение библиотек днк |
| AU2016357723A1 (en) * | 2015-11-16 | 2018-06-14 | Revere Biosensors, Llc | Systems and methods for identifying and distinguishing genetic samples |
| US10894990B2 (en) | 2016-05-17 | 2021-01-19 | Shoreline Biome, Llc | High throughput method for identification and sequencing of unknown microbial and eukaryotic genomes from complex mixtures |
| KR102505122B1 (ko) | 2016-08-25 | 2023-03-08 | 레졸루션 바이오사이언스, 인크. | Dna 샘플 중 게놈 카피 변화의 검출을 위한 방법 |
| GB201615233D0 (en) * | 2016-09-08 | 2016-10-26 | Bbi Solutions Oem Ltd | Solid Phase Conjugate |
| CA3063106A1 (en) | 2017-05-09 | 2018-11-15 | Revere Biosensors, Llc | Systems and methods for identifying and distinguishing genetic samples |
| US10876148B2 (en) | 2018-11-14 | 2020-12-29 | Element Biosciences, Inc. | De novo surface preparation and uses thereof |
| US10704094B1 (en) * | 2018-11-14 | 2020-07-07 | Element Biosciences, Inc. | Multipart reagents having increased avidity for polymerase binding |
| EP4103746A4 (en) * | 2020-02-14 | 2024-05-01 | Sequencing Health, Inc. | METHODS AND SYSTEMS FOR AMPLIFICATION AND SEQUENCING NUCLEIC ACIDS |
| US20220316003A1 (en) * | 2020-03-09 | 2022-10-06 | Illumina, Inc. | Methods for sequencing polynucleotides |
| MX2022014724A (es) * | 2020-09-14 | 2023-03-06 | Illumina Inc | Composiciones y metodos para amplificar polinucleótidos. |
| US11859241B2 (en) | 2021-06-17 | 2024-01-02 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| US11236388B1 (en) | 2021-06-17 | 2022-02-01 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| WO2023076833A1 (en) * | 2021-10-26 | 2023-05-04 | Singular Genomics Systems, Inc. | Multiplexed targeted amplification of polynucleotides |
| CA3223090A1 (en) * | 2022-03-31 | 2023-10-05 | Xiaoyu Ma | Paired-end re-synthesis using blocked p5 primers |
Family Cites Families (89)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4292503A (en) | 1979-05-14 | 1981-09-29 | Emerson Electric Co. | Split-band electric heater |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| EP0224126A3 (en) | 1985-11-25 | 1989-02-01 | The University of Calgary | Covalently linked complementary oligodeoxynucleotides as universal nucleic acid sequencing primer linkers |
| WO1989001050A1 (en) | 1987-07-31 | 1989-02-09 | The Board Of Trustees Of The Leland Stanford Junior University | Selective amplification of target polynucleotide sequences |
| SE8801070D0 (sv) | 1988-03-23 | 1988-03-23 | Pharmacia Ab | Method for immobilizing a dna sequence on a solid support |
| US6107023A (en) | 1988-06-17 | 2000-08-22 | Genelabs Technologies, Inc. | DNA amplification and subtraction techniques |
| GB8818020D0 (en) | 1988-07-28 | 1988-09-01 | Ici Plc | Method for amplification of nucleotide sequences |
| CA2004326A1 (en) | 1988-12-23 | 1990-06-23 | Nanibushan Dattagupta | Assay of sequences using amplified genes |
| US5629158A (en) | 1989-03-22 | 1997-05-13 | Cemu Bitecknik Ab | Solid phase diagnosis of medical conditions |
| US5547839A (en) | 1989-06-07 | 1996-08-20 | Affymax Technologies N.V. | Sequencing of surface immobilized polymers utilizing microflourescence detection |
| US5800992A (en) | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
| US5302509A (en) | 1989-08-14 | 1994-04-12 | Beckman Instruments, Inc. | Method for sequencing polynucleotides |
| JP3096722B2 (ja) | 1990-01-17 | 2000-10-10 | 日本ケミカルリサーチ株式会社 | ポリメラーゼ・チェーン・リアクション用プライマーの作製法およびそれを用いるｄｎａの増幅法 |
| US5118604A (en) | 1990-06-15 | 1992-06-02 | Yale University | Restriction-fragment DNA probes and probe clusters |
| WO1993004199A2 (en) | 1991-08-20 | 1993-03-04 | Scientific Generics Limited | Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids |
| ATE191510T1 (de) | 1991-09-24 | 2000-04-15 | Keygene Nv | Selektive restriktionsfragmentenamplifikation: generelles verfahren für dns-fingerprinting |
| WO1993017126A1 (en) | 1992-02-19 | 1993-09-02 | The Public Health Research Institute Of The City Of New York, Inc. | Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids |
| DE69327326T2 (de) | 1992-07-24 | 2001-08-16 | Diatech Pty Ltd | Vervielfältigungs- und detektionsprozess |
| RU2048522C1 (ru) | 1992-10-14 | 1995-11-20 | Институт белка РАН | Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления |
| JPH07203998A (ja) | 1994-01-26 | 1995-08-08 | Hamamatsu Photonics Kk | 核酸塩基配列決定方法 |
| EP0763135B1 (en) | 1994-05-28 | 2002-07-10 | Tepnel Medical Limited | Producing copies of nucleic acids |
| US5641658A (en) | 1994-08-03 | 1997-06-24 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid with two primers bound to a single solid support |
| US6090592A (en) | 1994-08-03 | 2000-07-18 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid on supports |
| US6060288A (en) | 1994-08-03 | 2000-05-09 | Mosaic Technologies | Method for performing amplification of nucleic acid on supports |
| WO1996032504A2 (en) | 1995-04-11 | 1996-10-17 | Trustees Of Boston University | Solid phase sequencing of biopolymers |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| DE19515552A1 (de) | 1995-04-27 | 1996-10-31 | Europ Lab Molekularbiolog | Simultane Sequenzierung von Nukleinsäuren |
| SE9504099D0 (sv) | 1995-11-16 | 1995-11-16 | Pharmacia Biotech Ab | A method of sequencing |
| US5935788A (en) | 1996-06-25 | 1999-08-10 | Lifespan Biosciences, Inc. | Subtractive hybridization techniques for identifying differentially expressed and commonly expressed nucleic acid |
| US5837466A (en) | 1996-12-16 | 1998-11-17 | Vysis, Inc. | Devices and methods for detecting nucleic acid analytes in samples |
| US20020061532A1 (en) | 1997-02-14 | 2002-05-23 | Mosaic Technologies, Inc. | Method and apparatus for performing amplification of nucleic acids on supports |
| EP1498494A3 (en) | 1997-04-01 | 2007-06-20 | Solexa Ltd. | Method of nucleic acid sequencing |
| ES2563643T3 (es) | 1997-04-01 | 2016-03-15 | Illumina Cambridge Limited | Método de secuenciación de ácido nucleico |
| US6534273B2 (en) * | 1997-05-02 | 2003-03-18 | Gen-Probe Incorporated | Two-step hybridization and capture of a polynucleotide |
| US6326489B1 (en) | 1997-08-05 | 2001-12-04 | Howard Hughes Medical Institute | Surface-bound, bimolecular, double-stranded DNA arrays |
| US6087099A (en) | 1997-09-08 | 2000-07-11 | Myriad Genetics, Inc. | Method for sequencing both strands of a double stranded DNA in a single sequencing reaction |
| US6511803B1 (en) | 1997-10-10 | 2003-01-28 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
| US20030022207A1 (en) | 1998-10-16 | 2003-01-30 | Solexa, Ltd. | Arrayed polynucleotides and their use in genome analysis |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| US6361947B1 (en) | 1998-10-27 | 2002-03-26 | Affymetrix, Inc. | Complexity management and analysis of genomic DNA |
| US6238868B1 (en) | 1999-04-12 | 2001-05-29 | Nanogen/Becton Dickinson Partnership | Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology |
| US20060275782A1 (en) * | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
| US20030108867A1 (en) | 1999-04-20 | 2003-06-12 | Chee Mark S | Nucleic acid sequencing using microsphere arrays |
| US6300070B1 (en) | 1999-06-04 | 2001-10-09 | Mosaic Technologies, Inc. | Solid phase methods for amplifying multiple nucleic acids |
| DE10051564A1 (de) | 2000-10-18 | 2002-08-01 | Axaron Bioscience Ag | Neue Verfahren zur parallelen Sequenzierung eines Nukleinsäuregemisches an einer Oberfläche |
| ATE317024T1 (de) | 1999-12-29 | 2006-02-15 | Keygene Nv | Verfahren zur erzeugung von oligonukleotiden, im besonderen zur detektion amplifizierter restriktionsfragmente, die durch aflp erhalten wurden |
| US7955794B2 (en) * | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
| WO2001079553A1 (en) | 2000-04-14 | 2001-10-25 | Lynx Therapeutics, Inc. | Method and compositions for ordering restriction fragments |
| AR031640A1 (es) | 2000-12-08 | 2003-09-24 | Applied Research Systems | Amplificacion isotermica de acidos nucleicos en un soporte solido |
| US7183050B2 (en) * | 2001-04-18 | 2007-02-27 | Krull Ulrich J | Gradient resolved information platform |
| EP1256632A3 (en) | 2001-05-07 | 2004-01-02 | Smithkline Beecham Corporation | High throughput polymorphism screening |
| WO2003056030A2 (en) | 2001-11-08 | 2003-07-10 | The Johns Hopkins University | Methods and systems of nucleic acid sequencing |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| US20040002090A1 (en) | 2002-03-05 | 2004-01-01 | Pascal Mayer | Methods for detecting genome-wide sequence variations associated with a phenotype |
| JP2005518811A (ja) | 2002-03-05 | 2005-06-30 | ソレックサ リミテッド | 表現型に関連するゲノム全域での配列変化を決定するための方法 |
| AU2003242605A1 (en) * | 2002-06-03 | 2003-12-19 | Pamgene B.V. | Novel high density arrays and methods for analyte analysis |
| US9388459B2 (en) | 2002-06-17 | 2016-07-12 | Affymetrix, Inc. | Methods for genotyping |
| DK3363809T3 (da) | 2002-08-23 | 2020-05-04 | Illumina Cambridge Ltd | Modificerede nukleotider til polynukleotidsekvensering |
| US20040126765A1 (en) | 2002-12-27 | 2004-07-01 | Adams Craig W. | Method and compositions for sequencing nucleic acid molecules |
| EP1590477B1 (en) | 2003-01-29 | 2009-07-29 | 454 Corporation | Methods of amplifying and sequencing nucleic acids |
| JP2006517798A (ja) | 2003-02-12 | 2006-08-03 | イェニソン スベンスカ アクティエボラーグ | 核酸配列用の方法及び手段 |
| GB0324456D0 (en) | 2003-10-20 | 2003-11-19 | Isis Innovation | Parallel DNA sequencing methods |
| EP1682680B2 (en) | 2003-10-31 | 2018-03-21 | AB Advanced Genetic Analysis Corporation | Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof |
| GB0400584D0 (en) | 2004-01-12 | 2004-02-11 | Solexa Ltd | Nucleic acid chacterisation |
| GB2412170A (en) | 2004-03-16 | 2005-09-21 | Plant Bioscience Ltd | A method for enriching for target nucleic acids in a sample |
| GB2413796B (en) | 2004-03-25 | 2006-03-29 | Global Genomics Ab | Methods and means for nucleic acid sequencing |
| US20070003924A1 (en) | 2004-06-18 | 2007-01-04 | The Ohio State University Research Foundation | Serial analysis of ribosomal and other microbial sequence tags |
| US20060078899A1 (en) | 2004-10-12 | 2006-04-13 | Scheffer Alicia F | Methods and compositions for reducing label variation in array-based comparative genome hybridization assays |
| WO2006110855A2 (en) | 2005-04-12 | 2006-10-19 | 454 Life Sciences Corporation | Methods for determining sequence variants using ultra-deep sequencing |
| EP1910537A1 (en) | 2005-06-06 | 2008-04-16 | 454 Life Sciences Corporation | Paired end sequencing |
| US8428882B2 (en) | 2005-06-14 | 2013-04-23 | Agency For Science, Technology And Research | Method of processing and/or genome mapping of diTag sequences |
| GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
| GB0514935D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Methods for sequencing a polynucleotide template |
| GB0514910D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Method for sequencing a polynucleotide template |
| GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
| DE602007009634D1 (de) | 2006-01-04 | 2010-11-18 | Si Lok | Verfahren zur zuordnung von nukleinsäuren und zur identifikation fein strukturierter variationen in nukleinsäuren sowie hilfsmittel dafür |
| CN101415839B (zh) | 2006-02-08 | 2012-06-27 | 亿明达剑桥有限公司 | 对多核苷酸模板进行测序的方法 |
| EP2021503A1 (en) | 2006-03-17 | 2009-02-11 | Solexa Ltd. | Isothermal methods for creating clonal single molecule arrays |
| US20070231823A1 (en) | 2006-03-23 | 2007-10-04 | Mckernan Kevin J | Directed enrichment of genomic DNA for high-throughput sequencing |
| US8241573B2 (en) | 2006-03-31 | 2012-08-14 | Illumina, Inc. | Systems and devices for sequence by synthesis analysis |
| US8178360B2 (en) | 2006-05-18 | 2012-05-15 | Illumina Cambridge Limited | Dye compounds and the use of their labelled conjugates |
| US8921073B2 (en) | 2006-06-23 | 2014-12-30 | Illumina, Inc. | Devices and systems for creation of DNA cluster arrays |
| US7754429B2 (en) | 2006-10-06 | 2010-07-13 | Illumina Cambridge Limited | Method for pair-wise sequencing a plurity of target polynucleotides |
| GB0712882D0 (en) | 2007-07-03 | 2007-08-15 | Leicester University Of | Nucleic acid amplification |
| WO2009032167A1 (en) | 2007-08-29 | 2009-03-12 | Illumina Cambridge | Method for sequencing a polynucleotide template |
| JP2009178159A (ja) * | 2007-11-05 | 2009-08-13 | Hitachi Plant Technologies Ltd | 核酸配列の検出方法及び核酸配列検出基板 |
| WO2010038042A1 (en) | 2008-10-02 | 2010-04-08 | Illumina Cambridge Ltd. | Nucleic acid sample enrichment for sequencing applications |
| MX346956B (es) | 2010-09-24 | 2017-04-06 | Univ Leland Stanford Junior | Captura directa, amplificación y secuenciación de objetivo adn usando cebadores inmovilizados. |
| WO2013117595A2 (en) * | 2012-02-07 | 2013-08-15 | Illumina Cambridge Limited | Targeted enrichment and amplification of nucleic acids on a support |
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| EP3133169A1 (en) | 2017-02-22 |
| EP2470669A1 (en) | 2012-07-04 |
| ES2595055T3 (es) | 2016-12-27 |
| US8999642B2 (en) | 2015-04-07 |
| US11142759B2 (en) | 2021-10-12 |
| US20120122737A1 (en) | 2012-05-17 |
| US20200239875A1 (en) | 2020-07-30 |
| EP3133169B1 (en) | 2019-10-16 |
| CY1117970T1 (el) | 2017-05-17 |
| US10597653B2 (en) | 2020-03-24 |
| CN102597256B (zh) | 2014-12-03 |
| LT2669387T (lt) | 2016-10-25 |
| US20150148263A1 (en) | 2015-05-28 |
| EP2470669B1 (en) | 2014-06-18 |
| CN102597256A (zh) | 2012-07-18 |
| PT2669387T (pt) | 2016-09-20 |
| HUE030765T2 (en) | 2017-05-29 |
| SI2669387T1 (sl) | 2017-01-31 |
| SMT201600319B (it) | 2016-11-10 |
| WO2011025477A1 (en) | 2011-03-03 |
| CA2770389C (en) | 2015-12-29 |
| DK3133169T3 (da) | 2019-12-16 |
| CA2770389A1 (en) | 2011-03-03 |
| EP2669387B1 (en) | 2016-07-20 |
| PL2669387T3 (pl) | 2016-12-30 |
| EP2470669A4 (en) | 2013-01-30 |
| DK2669387T3 (en) | 2016-09-19 |
| ES2763386T3 (es) | 2020-05-28 |
| EP2669387A1 (en) | 2013-12-04 |
| US9624489B2 (en) | 2017-04-18 |
| US20170283794A1 (en) | 2017-10-05 |
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