DE69327326T2 - Vervielfältigungs- und detektionsprozess - Google Patents

Vervielfältigungs- und detektionsprozess

Info

Publication number
DE69327326T2
DE69327326T2 DE69327326T DE69327326T DE69327326T2 DE 69327326 T2 DE69327326 T2 DE 69327326T2 DE 69327326 T DE69327326 T DE 69327326T DE 69327326 T DE69327326 T DE 69327326T DE 69327326 T2 DE69327326 T2 DE 69327326T2
Authority
DE
Germany
Prior art keywords
replacement
detection process
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69327326T
Other languages
English (en)
Other versions
DE69327326D1 (de
Inventor
Raymond Harris
Charles Morris
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Diatech Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diatech Pty Ltd filed Critical Diatech Pty Ltd
Application granted granted Critical
Publication of DE69327326D1 publication Critical patent/DE69327326D1/de
Publication of DE69327326T2 publication Critical patent/DE69327326T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
DE69327326T 1992-07-24 1993-07-26 Vervielfältigungs- und detektionsprozess Expired - Lifetime DE69327326T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPL370592 1992-07-24
PCT/AU1993/000379 WO1994002634A1 (en) 1992-07-24 1993-07-26 Amplification and detection process

Publications (2)

Publication Number Publication Date
DE69327326D1 DE69327326D1 (de) 2000-01-20
DE69327326T2 true DE69327326T2 (de) 2001-08-16

Family

ID=3776308

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69327326T Expired - Lifetime DE69327326T2 (de) 1992-07-24 1993-07-26 Vervielfältigungs- und detektionsprozess

Country Status (5)

Country Link
US (1) US5849544A (de)
EP (1) EP0656068B1 (de)
CA (1) CA2140877C (de)
DE (1) DE69327326T2 (de)
WO (1) WO1994002634A1 (de)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7582421B2 (en) * 1993-11-01 2009-09-01 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip
US6468742B2 (en) 1993-11-01 2002-10-22 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using bioelectronic microchip
US5480783A (en) * 1994-03-31 1996-01-02 The Perkin-Elmer Corporation Method for reducing background signals in DNA replication/detection assays
GB9408607D0 (en) * 1994-04-29 1994-06-22 Dynal As Assay
ATE196322T1 (de) * 1995-05-05 2000-09-15 Perkin Elmer Corp Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
CA2229226A1 (en) * 1995-08-14 1997-02-27 Abbott Laboratories All-in-one nucleic acid amplification assay
WO1997021832A1 (de) * 1995-12-08 1997-06-19 Evotec Biosystems Gmbh Verfahren zur bestimmung von nukleinsäuremolekülen in niedriger konzentration
EP0876510B1 (de) * 1995-12-22 2008-05-07 Dade Behring Marburg GmbH Homogene vervielfältigung und nachweis von nukleinsäuren
EP0892856B1 (de) * 1996-03-01 2005-11-09 E.I. Du Pont De Nemours And Company Methode zur vervielfältigung und zum nachweis eines gewünschten nukleinsäurefragments
US5817463A (en) * 1996-06-28 1998-10-06 Abbott Laboratories Nucleic acid primers and probes for detecting Mycoplasma pneumoniae
US6124092A (en) 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
GB9624165D0 (en) 1996-11-19 1997-01-08 Amdex A S Use of nucleic acids bound to carrier macromolecules
EP1591541B1 (de) 1997-04-01 2012-02-15 Illumina Cambridge Limited Verfahren zur Vervielfältigung von Nukleinsäuren
US5888737A (en) * 1997-04-15 1999-03-30 Lynx Therapeutics, Inc. Adaptor-based sequence analysis
IL124275A (en) * 1997-05-02 2002-03-10 Bio Merieux Vitek Inc A method to produce sequences of nucleic acids
US6558901B1 (en) * 1997-05-02 2003-05-06 Biomerieux Vitek Nucleic acid assays
DE19730359A1 (de) * 1997-07-15 1999-01-21 Boehringer Mannheim Gmbh Integriertes Verfahren und System zur Amplifizierung und zum Nachweis von Nukleinsäuren
US6485901B1 (en) * 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
WO2000058522A1 (en) * 1999-03-30 2000-10-05 Nanogen, Inc. Single nucleotide polymorphic discrimination by electronic dot blot assay on semiconductor microchips
US20030207295A1 (en) * 1999-04-20 2003-11-06 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7097973B1 (en) * 1999-06-14 2006-08-29 Alpha Mos Method for monitoring molecular species within a medium
US6709816B1 (en) * 1999-10-18 2004-03-23 Affymetrix, Inc. Identification of alleles
EP1096024A1 (de) * 1999-10-28 2001-05-02 Remacle, José Verfahren und Reagentiensatz zum Auswählen und/oder Quantifizieren von multiplen homologen Nukleinsäuresequenzen unter Verwendung von Sondenanordnungen
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) * 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
WO2001073122A1 (en) * 2000-03-28 2001-10-04 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip
US7998673B2 (en) * 2000-03-29 2011-08-16 Lgc Limited Hybridisation beacon and method of rapid sequence detection and discrimination
NZ521593A (en) * 2000-03-29 2004-11-26 Lgc Ltd Hybridisation beacon and method of rapid sequence detection and discrimination
GB0016836D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (1)
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
WO2003048732A2 (en) * 2001-02-07 2003-06-12 Illumina, Inc. Multiplexed methylation detection methods
US7601493B2 (en) * 2002-07-26 2009-10-13 Nanogen, Inc. Methods and apparatus for screening and detecting multiple genetic mutations
US20060105337A1 (en) * 2002-08-30 2006-05-18 Brian Warner Solid phase based nucleic acid assays combining high affinity and high specificity
DE10253337B4 (de) * 2002-11-14 2005-10-20 November Ag Molekulare Medizin Verfahren zum Nachweis einer Nukleinsäure
DE10253966B4 (de) * 2002-11-19 2005-03-24 Clondiag Chip Technologies Gmbh Microarray-basiertes Verfahren zur Amplifikation und Detektion von Nukleinsäuren in einem kontinuierlichen Prozess
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US7338763B2 (en) 2004-06-02 2008-03-04 Eppendorf Array Technologies S.A. Method and kit for the detection and/or quantification of homologous nucleotide sequences on arrays
US20060134650A1 (en) * 2004-12-21 2006-06-22 Illumina, Inc. Methylation-sensitive restriction enzyme endonuclease method of whole genome methylation analysis
EP1863908B1 (de) 2005-04-01 2010-11-17 Qiagen GmbH Reverse transkription und amplifikation von rna bei simultaner degradierung von dna
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
WO2007091077A1 (en) 2006-02-08 2007-08-16 Solexa Limited Method for sequencing a polynucleotide template
US9839909B2 (en) * 2006-07-28 2017-12-12 Diagnostics For The Real World, Ltd. Device, system and method for processing a sample
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
EP2121983A2 (de) 2007-02-02 2009-11-25 Illumina Cambridge Limited Verfahren zur kennzeichnung von proben und sequenzierung mehrerer nukleotidmatrizen
CA2691197C (en) 2007-06-21 2013-03-12 Gen-Probe Incorporated Instrument and receptacles for use in performing processes
US9707556B2 (en) 2007-08-17 2017-07-18 Diagnostics For The Real World, Ltd. Device, system and method for processing a sample
GB0806041D0 (en) * 2008-04-03 2008-05-14 Genomica S A Method for detection of herpesvirus in test sample
GB0814570D0 (en) 2008-08-08 2008-09-17 Diagnostics For The Real World Isolation of nucleic acid
WO2010038042A1 (en) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Nucleic acid sample enrichment for sequencing applications
HUE030765T2 (en) 2009-08-25 2017-05-29 Illumina Inc A method for selecting and amplifying polynucleotides
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
KR20110078185A (ko) * 2009-12-30 2011-07-07 삼성전자주식회사 폴리뉴클레오티드를 이용한 제품 인증 장치 및 방법
EP2543737B1 (de) * 2010-03-04 2017-08-02 K-Mac Bio Center Corp. Verfahren zum nachweis von nukleinsäuren durch förderung verzweigter dna-komplex-formierungen

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5635347A (en) * 1986-04-30 1997-06-03 Igen, Inc. Rapid assays for amplification products
WO1990010716A1 (en) * 1989-03-10 1990-09-20 Gene-Trak Systems Immobilized oligonucleotide probes and uses therefor
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
JPH03262499A (ja) * 1990-03-12 1991-11-22 Kosumitsuku:Kk ポリヌクレオチド検出方法およびｐｃｒ反応装置
JP2985446B2 (ja) * 1990-10-31 1999-11-29 東ソー株式会社 核酸の検出及び測定方法
WO1993004199A2 (en) * 1991-08-20 1993-03-04 Scientific Generics Limited Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids
EP0567635B1 (de) * 1991-11-15 2000-05-31 Igen International, Inc. Schnelle bestimmungsverfahren für amplifikationsprodukte

Also Published As

Publication number Publication date
EP0656068A1 (de) 1995-06-07
CA2140877A1 (en) 1994-02-03
WO1994002634A1 (en) 1994-02-03
EP0656068A4 (de) 1996-05-29
EP0656068B1 (de) 1999-12-15
DE69327326D1 (de) 2000-01-20
US5849544A (en) 1998-12-15
CA2140877C (en) 2008-11-18

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: DIATECH PTY. LTD., BRISBANE, QLD, AU

8327 Change in the person/name/address of the patent owner

Owner name: EPPENDORF AG, 22339 HAMBURG, DE