ATE196322T1 - Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay - Google Patents

Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay

Info

Publication number
ATE196322T1
ATE196322T1 AT96911592T AT96911592T ATE196322T1 AT E196322 T1 ATE196322 T1 AT E196322T1 AT 96911592 T AT96911592 T AT 96911592T AT 96911592 T AT96911592 T AT 96911592T AT E196322 T1 ATE196322 T1 AT E196322T1
Authority
AT
Austria
Prior art keywords
oligonucleotide
methods
reagents
probe
polymerase
Prior art date
Application number
AT96911592T
Other languages
English (en)
Inventor
Paul E Mayrand
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Application granted granted Critical
Publication of ATE196322T1 publication Critical patent/ATE196322T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT96911592T 1995-05-05 1996-04-05 Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay ATE196322T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43550995A 1995-05-05 1995-05-05
PCT/US1996/004693 WO1996034983A1 (en) 1995-05-05 1996-04-05 Methods and reagents for combined pcr amplification and hybridization probing assay

Publications (1)

Publication Number Publication Date
ATE196322T1 true ATE196322T1 (de) 2000-09-15

Family

ID=23728690

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96911592T ATE196322T1 (de) 1995-05-05 1996-04-05 Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay

Country Status (8)

Country Link
US (8) US5691146A (de)
EP (1) EP0826066B1 (de)
JP (3) JP4040087B2 (de)
AT (1) ATE196322T1 (de)
AU (1) AU693023B2 (de)
CA (1) CA2219891C (de)
DE (1) DE69610304T2 (de)
WO (1) WO1996034983A1 (de)

Families Citing this family (143)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6787304B1 (en) 1994-12-28 2004-09-07 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US20030165908A1 (en) * 1994-12-30 2003-09-04 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US5801155A (en) * 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
AU693023B2 (en) 1995-05-05 1998-06-18 Applied Biosystems, Llc Methods and reagents for combined PCR amplification and hybridization probing assay
US6706471B1 (en) * 1996-01-24 2004-03-16 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
WO1998002582A2 (en) * 1996-07-16 1998-01-22 Gen-Probe Incorporated Methods for detecting and amplifying nucleic acid sequences using modified oligonucleotides having increased target specific t¿m?
US7070925B1 (en) 1996-07-16 2006-07-04 Gen-Probe Incorporated Method for determining the presence of an RNA analyte in a sample using a modified oligonucleotide probe
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
DE19782089T1 (de) 1996-10-29 1999-12-23 Univ Nebraska At Lincoln Linco Verfahren zum Nachweisen von Punktmutationen in DNA unter Verwendung von Fluoreszenzenergieübergang
US6017702A (en) * 1996-12-05 2000-01-25 The Perkin-Elmer Corporation Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate
US6251591B1 (en) 1997-02-27 2001-06-26 Lorne Park Research, Inc. Quantitative method for detecting nucleotide concentration
US6060242A (en) * 1997-02-27 2000-05-09 Lorne Park Research, Inc. PNA diagnostic methods
US5846729A (en) * 1997-02-27 1998-12-08 Lorne Park Research, Inc. Assaying nucleotides in solution using a fluorescent intensity quenching effect
US7803528B1 (en) 1997-02-28 2010-09-28 Quest Diagnostics Incorporated Fluorescence energy transfer by competitive hybridization
JP3016759B2 (ja) * 1997-02-28 2000-03-06 スミスクライン・ビーチャム・コーポレイション 競争的ハイブリダイゼーションによる蛍光エネルギー転移
EP1666609B1 (de) * 1997-02-28 2012-09-26 Quest Diagnostics Investments Incorporated Fluoreszens-Energie-Transfer durch kompetitive Hybridisierung
AU9190098A (en) * 1997-09-18 1999-04-05 Erasmus Universiteit Rotterdam Detection of minimal residual disease in lymphoid malignancies
US5935791A (en) * 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
FR2769323B1 (fr) * 1997-10-08 2001-07-13 Suez Lyonnaise Des Eaux Moyens pour l'analyse qualitative et quantitative des populations microbiennes eventuellement presentes dans un echantillon
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
AU1366299A (en) 1997-10-27 1999-05-17 Boston Probes, Inc. Methods, kits and compositions pertaining to pna molecular beacons
US6080868A (en) 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
GB9803382D0 (en) * 1998-02-19 1998-04-15 Secr Defence Detection system
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6312902B1 (en) 1998-03-13 2001-11-06 Promega Corporation Nucleic acid detection
US6703211B1 (en) 1998-03-13 2004-03-09 Promega Corporation Cellular detection by providing high energy phosphate donor other than ADP to produce ATP
US7090975B2 (en) 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6270974B1 (en) 1998-03-13 2001-08-07 Promega Corporation Exogenous nucleic acid detection
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
US6277578B1 (en) 1998-03-13 2001-08-21 Promega Corporation Deploymerization method for nucleic acid detection of an amplified nucleic acid target
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
WO1999049293A2 (en) * 1998-03-24 1999-09-30 Boston Probes, Inc. Methods, kits and compositions pertaining to detection complexes
US6255050B1 (en) 1998-05-22 2001-07-03 Lorne Park Research, Inc. Dynamic hybridization system
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
DE69941333D1 (de) 1998-07-02 2009-10-08 Gen Probe Inc Molekulare fackeln
GB9819418D0 (en) * 1998-09-07 1998-10-28 Secr Defence Amplification method
US6153411A (en) 1998-10-30 2000-11-28 American Water Works Company, Inc. Methods and kits for detection of Cryptosporidium parvum using immunomagnetic separation and amplification
US7141417B1 (en) * 1999-02-25 2006-11-28 Thomas Jefferson University Compositions, kits, and methods relating to the human FEZ1 gene, a novel tumor suppressor gene
US6221635B1 (en) 1999-05-06 2001-04-24 The Wistar Institute Methods for solid-phase amplification of DNA template (SPADT) using multiarrays
US6645733B1 (en) 1999-06-25 2003-11-11 Ingeneus Corporation Fluorescent intensity method for assaying binding between proteins or peptides
US6692834B1 (en) * 1999-06-28 2004-02-17 Medtronic, Inc. Method for coating implantable devices
AU780156B2 (en) * 1999-09-22 2005-03-03 Wakunaga Pharmaceutical Co., Ltd Hybridization self-recognition type probe
WO2001038585A2 (en) * 1999-11-24 2001-05-31 The Regents Of The University Of California Polymer arrays and methods of using labeled probe molecules to identify and quantify target molecule expression
GB2359625B (en) * 1999-12-10 2004-10-20 Molecular Light Tech Res Ltd Monitoring oligonucleotide binding process using chemiluminescence quenching
US7354707B2 (en) * 2000-06-27 2008-04-08 National Institute Of Advanced Industrial Science And Technology Nucleic acid probes, method for determining concentrations of nucleic acid by using the probes, and method for analyzing data obtained by the method
CA2417986C (en) 2000-08-11 2013-11-26 University Of Utah Research Foundation Single-labeled oligonucleotide probes
CN1348096A (zh) 2000-10-10 2002-05-08 栾国彦 一种均相特异性检测核酸的探针及应用方法
JP2002125688A (ja) * 2000-10-30 2002-05-08 Tosoh Corp Ｃ型肝炎ウイルスの高感度検出のためのオリゴヌクレオチドおよび検出法
JP2002360257A (ja) * 2001-06-05 2002-12-17 Fuji Photo Film Co Ltd Ｄｎａマイクロアレイにおける遺伝子の検出法
EP1442142A4 (de) * 2001-10-19 2006-11-15 Proligo Llc Nukleinsäuresonden und verfahren zum nachweis und zur quantifizierung von nukleinsäureanalyten
US6946245B2 (en) * 2001-12-04 2005-09-20 Quest Diagnostics Investments Incorporated Oligonucleotides and methods for detecting hepatitis C viral nucleic acids
EP1485056B1 (de) * 2002-02-27 2011-04-06 Life Technologies Corporation Verfahren zur verwendung von mit fet markierten oligonucleotiden mit einer 3 -5-exonuclease-resistenten quencher-domäne und zusammensetzungen zur durchführung des verfahrens
US6818420B2 (en) * 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
DK1342794T3 (da) * 2002-03-05 2006-04-24 Epigenomics Ag Fremgangsmåde og anordning til at bestemme vævsspecificitet af fritflydende DNA i legemsvæsker
US7445893B2 (en) * 2002-04-12 2008-11-04 Primera Biosystems, Inc. Sampling method for amplification reaction analysis
US7081339B2 (en) * 2002-04-12 2006-07-25 Primera Biosystems, Inc. Methods for variation detection
US20070059690A1 (en) * 2002-05-31 2007-03-15 Amirul Islam "Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands"
JP2006510348A (ja) * 2002-07-18 2006-03-30 ザ・ボード・オブ・トラスティーズ・オブ・ザ・レランド・スタンフォード・ジュニア・ユニバーシティ 蛍光消光脱離基を用いる化学ライゲーションの検出
US7452712B2 (en) 2002-07-30 2008-11-18 Applied Biosystems Inc. Sample block apparatus and method of maintaining a microcard on a sample block
US7364848B2 (en) 2002-09-02 2008-04-29 Pamgene B.V. Integrated microarray analysis
US20040067492A1 (en) * 2002-10-04 2004-04-08 Allan Peng Reverse transcription on microarrays
US20050089875A1 (en) * 2002-11-21 2005-04-28 Sention Inc. Quantitative analysis of expression profiling information produced at various stages of an amplification process
EP3000899A1 (de) 2002-12-04 2016-03-30 Applied Biosystems, LLC Multiplexverstärkung von polynucleotiden
WO2004068112A2 (en) * 2003-01-28 2004-08-12 Gorilla Genomics, Inc. Hairpin primer amplification
US20040219533A1 (en) * 2003-04-29 2004-11-04 Jim Davis Biological bar code
US8679789B2 (en) * 2003-05-01 2014-03-25 Gen-Probe Incorporated Oligonucleotides comprising a molecular switch
WO2005029041A2 (en) * 2003-09-19 2005-03-31 Applera Corporation High density sequence detection methods and apparatus
US20060024690A1 (en) * 2003-09-19 2006-02-02 Kao H P Normalization of data using controls
WO2005049849A2 (en) 2003-11-14 2005-06-02 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
JP2007534308A (ja) * 2003-11-19 2007-11-29 アレロジック・バイオサイエンシズ・コーポレーション 複数のフルオロフォアで標識したオリゴヌクレオチド
US20090041782A1 (en) * 2003-12-30 2009-02-12 Takashi Ooshima Novel serotype streptococcus mutans and utilization of the same
US20050186606A1 (en) * 2004-02-11 2005-08-25 Schroeder Benjamin G. Methods and compositions for detecting nucleic acids
EP2208797A3 (de) 2004-03-01 2010-11-24 Applied Biosystems, LLC Verfahren, Zusammensetzungen und Kits für Polynukleotidamplifikation
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
CA2566949C (en) * 2004-05-20 2015-03-24 Warnex Research Inc. Polynucleotides for the detection of escherichia coli o157:h7 and escherichia coli o157:nm verotoxin producers
US20060003337A1 (en) * 2004-06-30 2006-01-05 John Brandis Detection of small RNAS
EP1799849A4 (de) 2004-09-07 2008-07-09 Telethon Inst For Child Health Mittel zur behandlung oder vorbeugung einer allergischen erkrankung
ATE482288T1 (de) 2004-11-18 2010-10-15 Eppendorf Array Technologies Echtzeit-pcr von zielen auf einem mikroarray
US20090068650A1 (en) * 2005-02-11 2009-03-12 Southern Illinois University Metabolic Primers for the Detection of (Per) Chlorate-Reducing Bacteria and Methods of Use Thereof
EP1853732A2 (de) * 2005-02-28 2007-11-14 Gen-Probe Incorporated Präparate und verfahren zur probenmessung durch eine umschaltbare nukleinsäuren-sonde
US8263330B1 (en) 2005-03-08 2012-09-11 Quest Diagnostics Investments Incorporated Detection of Mycobacterium tuberculosis complex nucleic acids
US7932081B2 (en) 2005-03-10 2011-04-26 Gen-Probe Incorporated Signal measuring system for conducting real-time amplification assays
US7776567B2 (en) * 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7601498B2 (en) * 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
CA2601554A1 (en) 2005-05-20 2006-11-30 Integrated Dna Technologies, Inc. Compounds and methods for labeling oligonucleotides
GB0514889D0 (en) 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
WO2007058898A2 (en) * 2005-11-10 2007-05-24 Panomics, Inc. Detection of nucleic acids through amplification of surrogate nucleic acids
EP1989326A4 (de) 2006-01-17 2009-09-30 Health Research Inc Heteroduplex-verfolgungstest
US7824858B2 (en) * 2006-01-23 2010-11-02 Quest Diagnostics Investments Incorporated Assay for mycobacterium avium/intracellulare nucleic acid
US8133984B2 (en) * 2006-03-16 2012-03-13 Pentabase Aps Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids
BRPI0709282B8 (pt) 2006-03-29 2021-05-25 Merial Ltd vacina contra estreptococos
US8078264B2 (en) 2006-07-11 2011-12-13 Case Western Reserve University Intra-operative molecular imaging
US7700756B2 (en) * 2006-07-27 2010-04-20 Southern Illinois University Metabolic primers for the detection of perchlorate-reducing bacteria and methods of use thereof
WO2008021446A2 (en) * 2006-08-15 2008-02-21 Genetag Technology, Inc. Probe-antiprobe compositions and methods for dna or rna detection
CA2668457C (en) * 2006-11-02 2016-10-04 Veridex, Llc Imaging of activated vascular endothelium using immunomagnetic mri contrast agents
US9657337B2 (en) * 2006-12-29 2017-05-23 Honeywell International Inc. Reaction buffer for microarray
US9271653B2 (en) 2007-06-12 2016-03-01 Case Western Reserve University Intra-operative molecular imaging
DE102007029772B4 (de) 2007-06-22 2011-12-08 Aj Innuscreen Gmbh Verfahren und Schnelltest zum Nachweis spezifischer Nukleinsäuresequenzen
IL184478A (en) * 2007-07-08 2017-07-31 Hadasit Medical Res Services & Development Ltd Preparations, methods and kits for identifying carriers of mutations in genes 1brca and 2brca and for the early detection of cancers associated with mutations in these genes
EP2107125A1 (de) 2008-03-31 2009-10-07 Eppendorf Array Technologies SA (EAT) Echtzeit-PCR von Targets auf einem Mikroarray
US20100143901A1 (en) * 2008-12-09 2010-06-10 Roche Molecular Systems, Inc. Nuclease-Free Real-Time Detection of Nucleic Acids
WO2010135917A1 (zh) 2009-05-26 2010-12-02 厦门大学 一种检测核酸序列变异的方法
WO2011054644A1 (en) 2009-10-14 2011-05-12 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. A low density lipoprotein-related protein 1 splice variant as cancer marker
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
US8916345B2 (en) 2010-03-26 2014-12-23 Integrated Dna Technologies, Inc. Methods for enhancing nucleic acid hybridization
DE102010003782B4 (de) 2010-04-08 2023-09-28 Ist Innuscreen Gmbh Vorrichtung zum Nachweis von Nukleinsäuren
DE102010003781B4 (de) * 2010-04-08 2012-08-16 Aj Innuscreen Gmbh Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen
US9234249B2 (en) 2010-07-12 2016-01-12 Gen-Probe Incorporated Compositions and assays to detect swine H1N1 influenza A virus, seasonal H1 influenza A virus and seasonal H3 influenza A virus nucleic acids
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
DE102010033107A1 (de) 2010-08-02 2012-02-02 Aj Innuscreen Gmbh Nachweis spezifischer Nukleinsäuresequenzen mittels Fluoreszenzlöschung
WO2012016936A1 (de) 2010-07-31 2012-02-09 Aj Innuscreen Gmbh Nachweis spezifischer nukleinsäuresequenzen mittels fluoreszenz - quenching
EP2896696B1 (de) 2010-09-07 2017-12-27 Integrated Dna Technologies, Inc. Modifikationen für Antisense-Verbindungen
US9051606B2 (en) 2010-09-10 2015-06-09 Qiagen Gaithersburg, Inc. Methods and compositions for nucleic acid detection
CA2812512A1 (en) 2010-09-29 2012-04-05 Mor Research Applications Ltd. Prognostic methods, compositions and kits for prediction of acute lymphoblastic leukemia (all) relapse
CN103403533B (zh) 2011-02-24 2017-02-15 简.探针公司 用于分辨光信号检测器中不同调制频率的光信号的系统和方法
DE102011078342A1 (de) 2011-06-29 2013-01-03 Aj Innuscreen Gmbh Rehybridisierendes Sondensystem zur qualitativen und quantitativen Messung von spezifischen Nukleinsäuren in Echtzeit
WO2013030841A1 (en) 2011-09-04 2013-03-07 Yissum Research Development Company Of The Hebrew Universitiy Of Jerusalem Ltd. Prognostic methods and compositions for predicting interferon treatment eficacy in a subject
EP4361608A3 (de) 2012-02-03 2024-07-24 California Institute of Technology Signalkodierung und -dekodierung in multiplexierten biochemischen assays
WO2013131083A1 (en) 2012-03-02 2013-09-06 Winthrop-University Hospital METHOD FOR USING PROBE BASED PCR DETECTION TO MEASURE THE LEVELS OF CIRCULATING DEMETHYLATED β CELL DERIVED DNA AS A MEASURE OF β CELL LOSS IN DIABETES
US9133509B2 (en) 2012-03-22 2015-09-15 Lgc Genomics Limited Polymerase chain reaction detection system
WO2013168162A1 (en) 2012-05-09 2013-11-14 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Clustered single nucleotide polymorphisms in the human acetylcholinesterase gene and uses thereof in diagnosis and therapy
EP3901243A1 (de) 2012-08-03 2021-10-27 California Institute of Technology Multiplexing und quantifizierung in pcr mit reduzierter hardware und reduzierten anforderungen
PL2712790T3 (pl) 2012-09-26 2016-03-31 3A Composites Gmbh Wypukły dach pojazdu z elementami wzmacniającymi i tłumiącymi
CN103114131B (zh) 2012-11-30 2018-10-02 珠海市坤元农业科技有限公司 一种引物中部序列干扰pcr技术
AU2013202805B2 (en) 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
US20160265058A1 (en) 2013-11-01 2016-09-15 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Diagnostic methods and kits for determining a personalized treatment regimen for a subject suffering from a pathologic disorder
JP6499656B2 (ja) 2013-11-22 2019-04-10 オリオン ディアグノスティカ オサケ ユキチュア 鎖侵入に基づく増幅による核酸の検出
CL2015000980A1 (es) 2014-04-18 2016-07-01 Upstartdna Co Ltd Standard Entity Métodos para detectar patógenos en peces de agua fría.
GB201410022D0 (en) 2014-06-05 2014-07-16 Orion Diagnostica Oy Method
US20170268070A1 (en) 2014-07-30 2017-09-21 Mor Research Applications Ltd. Prognostic methods and systems of treatment for acute lymphoblastic leukemia
US9856521B2 (en) * 2015-01-27 2018-01-02 BioSpyder Technologies, Inc. Ligation assays in liquid phase
US11091810B2 (en) * 2015-01-27 2021-08-17 BioSpyder Technologies, Inc. Focal gene expression profiling of stained FFPE tissues with spatial correlation to morphology
US10683534B2 (en) * 2015-01-27 2020-06-16 BioSpyder Technologies, Inc. Ligation assays in liquid phase
CN108699608A (zh) 2015-10-09 2018-10-23 基因前沿公司 用于预测和诊断人巨细胞病毒(hCMV)先天性传播的方法和试剂盒
WO2017218777A1 (en) 2016-06-17 2017-12-21 California Institute Of Technology Nucleic acid reactions and related methods and compositions
JP6853523B2 (ja) * 2016-07-27 2021-03-31 国立大学法人京都大学 ヘリカーゼを用いたｐｃｒ
US12454720B2 (en) 2018-04-17 2025-10-28 ChromaCode, Inc. Methods and systems for multiplex analysis
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
WO2020069658A1 (zh) 2018-10-05 2020-04-09 福又达生物科技股份有限公司 鱼病原体检测方法
IT202100009338A1 (it) 2021-04-14 2022-10-14 Celera Digital S R L Sistema di interconnessione e predizione aziendale e metodo di funzionamento di sistema di segnalazione
DK181862B1 (en) 2022-03-21 2025-02-24 Samplix Aps Method and kit for targeted enrichment of large dna molecules for long-read sequencing using facs or microfluidic partitioning

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4220450A (en) 1978-04-05 1980-09-02 Syva Company Chemically induced fluorescence immunoassay
US4766064A (en) 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay employing polyether and diagnostic kit
CA1273552A (en) * 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
ATE88761T1 (de) 1986-01-10 1993-05-15 Amoco Corp Kompetitiver homogener test.
US5635347A (en) 1986-04-30 1997-06-03 Igen, Inc. Rapid assays for amplification products
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5273879A (en) * 1987-07-23 1993-12-28 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
WO1990003445A1 (en) 1988-09-30 1990-04-05 The Salk Institute For Biological Studies Target nucleic acid amplification/detection systems
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
WO1992014845A1 (en) * 1991-02-26 1992-09-03 Worcester Foundation For Experimental Biology Diagnosing cystic fibrosis and other genetic diseases using fluorescence resonance energy transfer (fret)
ES2091976T3 (es) * 1991-06-20 1996-11-16 Hoffmann La Roche Metodos perfeccionados para la amplificacion del acido nucleico.
CA2218875C (en) 1991-07-23 2000-11-07 The Research Foundation Of State University Of New York Improvements in the in situ pcr
EP0549107B1 (de) 1991-10-11 1997-12-29 BEHRINGWERKE Aktiengesellschaft Verfahren zur Herstellung eines Polynukleotids zum Gebrauch in "single-primer" Amplifizierung und Phosphorothioat-enthaltende Oligonukleotide als Primer in Nukleinsäureamplifizierung
DK0567635T3 (da) * 1991-11-15 2000-09-25 Igen Int Inc Hurtige assays for amplifikationsprodukter
US5332659A (en) 1992-04-09 1994-07-26 The United States Of America As Represented By The Secretary Of The Navy Light emission-or absorbance-based binding assays for polynucleic acids
US6020124A (en) 1992-04-27 2000-02-01 Trustees Of Dartmouth College Detection of soluble gene sequences in biological fluids
GB9211979D0 (en) 1992-06-05 1992-07-15 Buchard Ole Uses of nucleic acid analogues
WO1994002634A1 (en) 1992-07-24 1994-02-03 University Of South Australia Amplification and detection process
WO1994003472A1 (en) * 1992-08-04 1994-02-17 Gen-Probe Incorporated Nucleic acid sequence amplification
EP0599388B1 (de) 1992-11-20 2000-08-02 Koninklijke Philips Electronics N.V. Halbleitervorrichtung mit einem programmierbaren Element
JPH06153997A (ja) * 1992-11-27 1994-06-03 Canon Inc 検出信号増幅による標的核酸の検出方法
EP0601889A2 (de) * 1992-12-10 1994-06-15 Maine Medical Center Research Institute Nukleinsäure-Sonden
US5364790A (en) 1993-02-16 1994-11-15 The Perkin-Elmer Corporation In situ PCR amplification system
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
GB9311386D0 (en) 1993-06-02 1993-07-21 Pna Diagnostics As Nucleic acid analogue assay procedures
DE69422678T2 (de) * 1993-10-12 2001-02-22 Matsushita Electric Industrial Co., Ltd. Verschlüsselungssystem, Verschlüsselungsgerät und Entschlüsselungsgerät
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
PT728218E (pt) 1993-11-12 2008-04-08 Phri Properties Inc Sondas de hibridação para detecção de ácidos nucleicos, troncos universais, métodos e estojos
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5629178A (en) 1994-10-28 1997-05-13 Genetics & Ivf Institute Method for enhancing amplification in the polymerase chain reaction employing peptide nucleic acid (PNA)
IE72524B1 (en) 1994-11-04 1997-04-23 Elan Med Tech Analyte-controlled liquid delivery device and analyte monitor
AU693023B2 (en) * 1995-05-05 1998-06-18 Applied Biosystems, Llc Methods and reagents for combined PCR amplification and hybridization probing assay
CA2229226A1 (en) 1995-08-14 1997-02-27 Abbott Laboratories All-in-one nucleic acid amplification assay

Also Published As

Publication number Publication date
US20030215826A1 (en) 2003-11-20
EP0826066A1 (de) 1998-03-04
US6485903B1 (en) 2002-11-26
JP4040519B2 (ja) 2008-01-30
US20110275071A1 (en) 2011-11-10
DE69610304T2 (de) 2001-04-05
US20050227247A1 (en) 2005-10-13
US7847076B2 (en) 2010-12-07
WO1996034983A1 (en) 1996-11-07
CA2219891C (en) 2002-01-29
US20080050742A1 (en) 2008-02-28
US7241596B2 (en) 2007-07-10
US6395518B1 (en) 2002-05-28
US20090258351A1 (en) 2009-10-15
EP0826066B1 (de) 2000-09-13
DE69610304D1 (de) 2000-10-19
US5691146A (en) 1997-11-25
JP2005110666A (ja) 2005-04-28
CA2219891A1 (en) 1996-11-07
JPH11504219A (ja) 1999-04-20
JP4040087B2 (ja) 2008-01-30
US7413708B2 (en) 2008-08-19
JP2004000185A (ja) 2004-01-08
AU693023B2 (en) 1998-06-18
AU5442896A (en) 1996-11-21

Similar Documents

Publication Publication Date Title
DE69610304D1 (de) Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
JP2870696B2 (ja) Ｄｎａ配列決定
DE69838210T2 (de) Markierter Primer, geeignet für die Detektion von Nukleinsäuren
US5885775A (en) Methods for determining sequences information in polynucleotides using mass spectrometry
US5364759B1 (en) Dna typing with short tandem repeat polymorphisms and indentification of polymorphic short tandem repeats
ATE282716T1 (de) Verfahren zur amplifikation von nukleinsäuresequenzen
CA2308599A1 (en) Dna polymorphism identity determination using flow cytometry
ATE398187T1 (de) Verfahren zum nachweis von ziel-nukleinsäuren mittels pcr
WO1993016094A3 (en) Applications of fluorescent n-nucleosides and fluorescent structural analogs of n-nucleosides
ATE185843T1 (de) Nukleinsäure-sequenzanalyse durch die methode der parallelen primerextension
WO2002008414A1 (en) Novel nucleic acid probes and method of assaying nucleic acid by using the same
KR970028548A (ko) 핵산의 형광 편광도 검출방법
FI953445A0 (fi) Menetelmä nukleiinihappojen herkkään osoitukseen
KR900018376A (ko) 리보핵산(rna)의 합성방법
DE69417863D1 (de) Verbessertes verfahren für die quantifizierung von nukleinsäuren
Fairfield et al. On the processivity of DNA replication
EP0741799B1 (de) Methode zur sequenzierung kurzer oligonukleotide
DE60133534T2 (de) Ein homogenes verfahren zur detektion eines polynukleotids, mit beispielsweise abspaltbarer lanthanidchelat markierung
WO2002088379A3 (en) Methods and compositions for utilizing changes of hybridization signals during approach to equilibrium
GB2295228A (en) Selective amplification of DNA having a plurality of sites for a specific endonuclease
EP1241267A8 (de) Enzym-Aktivitätstest mittels fluoreszenzmarkierter Oligonukleotid-Substrate
Basagoudanavar et al. Random amplification of polymorphic DNA fingerprinting of Trypanosoma evansi
DE19757300A1 (de) Nachweis von Harnblasenkarzinom in einer Urinprobe
RU2084534C1 (ru) Способ получения двуцепочечной нуклеиновой кислоты
DE69424541T2 (de) Enzymatische ligation von 3'-aminosubstituierten oligonukleotiden

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties