ATE196322T1 - Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay - Google Patents

Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay

Info

Publication number
ATE196322T1
ATE196322T1 AT96911592T AT96911592T ATE196322T1 AT E196322 T1 ATE196322 T1 AT E196322T1 AT 96911592 T AT96911592 T AT 96911592T AT 96911592 T AT96911592 T AT 96911592T AT E196322 T1 ATE196322 T1 AT E196322T1
Authority
AT
Austria
Prior art keywords
oligonucleotide
methods
reagents
probe
polymerase
Prior art date
Application number
AT96911592T
Other languages
English (en)
Inventor
Paul E Mayrand
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Application granted granted Critical
Publication of ATE196322T1 publication Critical patent/ATE196322T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT96911592T 1995-05-05 1996-04-05 Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay ATE196322T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43550995A 1995-05-05 1995-05-05
PCT/US1996/004693 WO1996034983A1 (en) 1995-05-05 1996-04-05 Methods and reagents for combined pcr amplification and hybridization probing assay

Publications (1)

Publication Number Publication Date
ATE196322T1 true ATE196322T1 (de) 2000-09-15

Family

ID=23728690

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96911592T ATE196322T1 (de) 1995-05-05 1996-04-05 Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay

Country Status (8)

Country Link
US (8) US5691146A (de)
EP (1) EP0826066B1 (de)
JP (3) JP4040087B2 (de)
AT (1) ATE196322T1 (de)
AU (1) AU693023B2 (de)
CA (1) CA2219891C (de)
DE (1) DE69610304T2 (de)
WO (1) WO1996034983A1 (de)

Families Citing this family (143)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6787304B1 (en) 1994-12-28 2004-09-07 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US20030165908A1 (en) * 1994-12-30 2003-09-04 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US5801155A (en) * 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
ATE196322T1 (de) 1995-05-05 2000-09-15 Perkin Elmer Corp Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
US6706471B1 (en) * 1996-01-24 2004-03-16 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US7070925B1 (en) 1996-07-16 2006-07-04 Gen-Probe Incorporated Method for determining the presence of an RNA analyte in a sample using a modified oligonucleotide probe
US6130038A (en) * 1996-07-16 2000-10-10 Gen-Probe Incorporated Method for amplifying target nucleic acids using modified primers
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
GB2333597B (en) 1996-10-29 2001-04-18 Univ Nebraska At Lincoln Method for detecting point mutations in dna utilizing fluorescence energy transfer
US6017702A (en) * 1996-12-05 2000-01-25 The Perkin-Elmer Corporation Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate
US5846729A (en) * 1997-02-27 1998-12-08 Lorne Park Research, Inc. Assaying nucleotides in solution using a fluorescent intensity quenching effect
US6251591B1 (en) 1997-02-27 2001-06-26 Lorne Park Research, Inc. Quantitative method for detecting nucleotide concentration
US6060242A (en) * 1997-02-27 2000-05-09 Lorne Park Research, Inc. PNA diagnostic methods
EP1666609B1 (de) * 1997-02-28 2012-09-26 Quest Diagnostics Investments Incorporated Fluoreszens-Energie-Transfer durch kompetitive Hybridisierung
JP3016759B2 (ja) * 1997-02-28 2000-03-06 スミスクライン・ビーチャム・コーポレイション 競争的ハイブリダイゼーションによる蛍光エネルギー転移
US7803528B1 (en) 1997-02-28 2010-09-28 Quest Diagnostics Incorporated Fluorescence energy transfer by competitive hybridization
WO1999014366A2 (en) * 1997-09-18 1999-03-25 Erasmus Universiteit Rotterdam Detection of minimal residual disease in lymphoid malignancies
US5935791A (en) * 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
FR2769323B1 (fr) * 1997-10-08 2001-07-13 Suez Lyonnaise Des Eaux Moyens pour l'analyse qualitative et quantitative des populations microbiennes eventuellement presentes dans un echantillon
US6485901B1 (en) * 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
EP1025120B1 (de) 1997-10-27 2010-08-18 Boston Probes, Inc. Sich auf "pna molecular beacons" beziehende verfahren, testsätze und zusammensetzungen
US6080868A (en) 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
GB9803382D0 (en) * 1998-02-19 1998-04-15 Secr Defence Detection system
US6270974B1 (en) 1998-03-13 2001-08-07 Promega Corporation Exogenous nucleic acid detection
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6277578B1 (en) 1998-03-13 2001-08-21 Promega Corporation Deploymerization method for nucleic acid detection of an amplified nucleic acid target
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
US6703211B1 (en) 1998-03-13 2004-03-09 Promega Corporation Cellular detection by providing high energy phosphate donor other than ADP to produce ATP
US7090975B2 (en) 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6312902B1 (en) 1998-03-13 2001-11-06 Promega Corporation Nucleic acid detection
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
US6361942B1 (en) 1998-03-24 2002-03-26 Boston Probes, Inc. Method, kits and compositions pertaining to detection complexes
US6255050B1 (en) 1998-05-22 2001-07-03 Lorne Park Research, Inc. Dynamic hybridization system
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
AU762728B2 (en) * 1998-07-02 2003-07-03 Gen-Probe Incorporated Molecular torches
GB9819418D0 (en) * 1998-09-07 1998-10-28 Secr Defence Amplification method
US6153411A (en) 1998-10-30 2000-11-28 American Water Works Company, Inc. Methods and kits for detection of Cryptosporidium parvum using immunomagnetic separation and amplification
US7141417B1 (en) 1999-02-25 2006-11-28 Thomas Jefferson University Compositions, kits, and methods relating to the human FEZ1 gene, a novel tumor suppressor gene
US6221635B1 (en) 1999-05-06 2001-04-24 The Wistar Institute Methods for solid-phase amplification of DNA template (SPADT) using multiarrays
US6645733B1 (en) 1999-06-25 2003-11-11 Ingeneus Corporation Fluorescent intensity method for assaying binding between proteins or peptides
US6692834B1 (en) * 1999-06-28 2004-02-17 Medtronic, Inc. Method for coating implantable devices
EP1215498A4 (de) * 1999-09-22 2005-04-20 Wakunaga Pharma Co Ltd Selbsterkennende hybridisierungssonde
WO2001038585A2 (en) * 1999-11-24 2001-05-31 The Regents Of The University Of California Polymer arrays and methods of using labeled probe molecules to identify and quantify target molecule expression
GB2359625B (en) * 1999-12-10 2004-10-20 Molecular Light Tech Res Ltd Monitoring oligonucleotide binding process using chemiluminescence quenching
WO2002008414A1 (en) * 2000-06-27 2002-01-31 National Institute Of Advanced Industrial Science And Technology Novel nucleic acid probes and method of assaying nucleic acid by using the same
EP1307592B1 (de) 2000-08-11 2006-07-05 University of Utah Research Foundation Einfach markierte oligonukleotidsonden
CN1348096A (zh) 2000-10-10 2002-05-08 栾国彦 一种均相特异性检测核酸的探针及应用方法
JP2002125688A (ja) * 2000-10-30 2002-05-08 Tosoh Corp Ｃ型肝炎ウイルスの高感度検出のためのオリゴヌクレオチドおよび検出法
JP2002360257A (ja) * 2001-06-05 2002-12-17 Fuji Photo Film Co Ltd Ｄｎａマイクロアレイにおける遺伝子の検出法
WO2003043402A2 (en) * 2001-10-19 2003-05-30 Proligo Llc Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes
US6946245B2 (en) 2001-12-04 2005-09-20 Quest Diagnostics Investments Incorporated Oligonucleotides and methods for detecting hepatitis C viral nucleic acids
WO2003072051A2 (en) * 2002-02-27 2003-09-04 Biosource International Inc. Methods of using fet labeled oligonucleotides that include a 3' 5' exonuclease resistant quencher domain and compositions for practicing the same
US6818420B2 (en) * 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
DK1342794T3 (da) * 2002-03-05 2006-04-24 Epigenomics Ag Fremgangsmåde og anordning til at bestemme vævsspecificitet af fritflydende DNA i legemsvæsker
US7445893B2 (en) * 2002-04-12 2008-11-04 Primera Biosystems, Inc. Sampling method for amplification reaction analysis
US7081339B2 (en) * 2002-04-12 2006-07-25 Primera Biosystems, Inc. Methods for variation detection
JP4457001B2 (ja) * 2002-05-31 2010-04-28 セクレタリー・デパートメント・オブ・アトミック・エナジー ドナー／アクセプター部分が相補鎖上となる標的核酸検出のｍｅｔ／ｆｒｅｔに基づく方法
EP1543156A4 (de) * 2002-07-18 2005-12-21 Univ Leland Stanford Junior Nachweis chemischer ligation unter verwendung von fluoreszenzquencher-abgangsgruppen
US7452712B2 (en) 2002-07-30 2008-11-18 Applied Biosystems Inc. Sample block apparatus and method of maintaining a microcard on a sample block
US7364848B2 (en) 2002-09-02 2008-04-29 Pamgene B.V. Integrated microarray analysis
US20040067492A1 (en) * 2002-10-04 2004-04-08 Allan Peng Reverse transcription on microarrays
US20050089875A1 (en) * 2002-11-21 2005-04-28 Sention Inc. Quantitative analysis of expression profiling information produced at various stages of an amplification process
EP1594975A4 (de) 2002-12-04 2006-08-02 Applera Corp Multiplex-amplifikation von polynukleotiden
US20040259116A1 (en) * 2003-01-28 2004-12-23 Gorilla Genomics, Inc. Hairpin primer amplification
US20040219533A1 (en) * 2003-04-29 2004-11-04 Jim Davis Biological bar code
JP2006525027A (ja) 2003-05-01 2006-11-09 ジェン−プロウブ インコーポレイテッド 分子スイッチを含むオリゴヌクレオチド
US20060024690A1 (en) * 2003-09-19 2006-02-02 Kao H P Normalization of data using controls
US20050112634A1 (en) * 2003-09-19 2005-05-26 Woudenberg Timothy M. High density sequence detection methods and apparatus
US7439341B2 (en) 2003-11-14 2008-10-21 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
JP2007534308A (ja) * 2003-11-19 2007-11-29 アレロジック・バイオサイエンシズ・コーポレーション 複数のフルオロフォアで標識したオリゴヌクレオチド
US20090041782A1 (en) * 2003-12-30 2009-02-12 Takashi Ooshima Novel serotype streptococcus mutans and utilization of the same
WO2005077125A2 (en) * 2004-02-11 2005-08-25 Applera Corporation Methods and compositions for detecting nucleic acids
EP1721011A1 (de) 2004-03-01 2006-11-15 Applera Corporation Verfahren, zusammensetzungen und kits zur verwendung bei der polynukleotidamplifikation
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
ATE557090T1 (de) * 2004-05-20 2012-05-15 Aes Chemunex S A Polynukleotide zum nachweis von escherichia coli o157:h7- und escherichia coli o157:nm- verotoxinproduzenten
US20060003337A1 (en) * 2004-06-30 2006-01-05 John Brandis Detection of small RNAS
JP2008512099A (ja) 2004-09-07 2008-04-24 テレソン インスティテュート フォー チャイルド ヘルス リサーチ アレルギー性疾患の処置または予防のための物質
ATE549420T1 (de) 2004-11-18 2012-03-15 Eppendorf Array Tech Sa Echtzeit-pcr von nukleinsäuren auf einem mikroarray
WO2006088445A2 (en) * 2005-02-11 2006-08-24 Southern Illinois University Metabolic primers for the detection of (per)chlorate-reducing bacteria and methods of use thereof
EP1853732A2 (de) * 2005-02-28 2007-11-14 Gen-Probe Incorporated Präparate und verfahren zur probenmessung durch eine umschaltbare nukleinsäuren-sonde
US8263330B1 (en) 2005-03-08 2012-09-11 Quest Diagnostics Investments Incorporated Detection of Mycobacterium tuberculosis complex nucleic acids
WO2006099255A2 (en) 2005-03-10 2006-09-21 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
US7601498B2 (en) * 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
US7776567B2 (en) * 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
WO2006127507A2 (en) * 2005-05-20 2006-11-30 Integrated Dna Technologies, Inc. Compounds and methods for labeling oligonucleotides
GB0514889D0 (en) 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
EP1951905B1 (de) 2005-11-10 2012-04-11 Affymetrix, Inc. Nachweis von nukleinsäuren über amplifikation von stellvertreternukleinsäuren
CA2637600A1 (en) 2006-01-17 2007-07-26 Health Research, Inc. Heteroduplex tracking assay
US7824858B2 (en) 2006-01-23 2010-11-02 Quest Diagnostics Investments Incorporated Assay for mycobacterium avium/intracellulare nucleic acid
WO2007104318A2 (en) * 2006-03-16 2007-09-20 Pentabase Aps Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids
WO2007115059A2 (en) 2006-03-29 2007-10-11 Merial Limited Vaccine against streptococci
US8078264B2 (en) 2006-07-11 2011-12-13 Case Western Reserve University Intra-operative molecular imaging
US7700756B2 (en) * 2006-07-27 2010-04-20 Southern Illinois University Metabolic primers for the detection of perchlorate-reducing bacteria and methods of use thereof
EP2059614A4 (de) * 2006-08-15 2010-06-30 Zygene Biotech Llc Sonden-gegensonden-zusammensetzungen und verfahren für dna- oder rna-nachweis
JP5350257B2 (ja) * 2006-11-02 2013-11-27 ベリデックス・エルエルシー 免疫磁気ｍｒｉ造影剤を用いた活性化された血管内皮の画像化
WO2008080254A1 (en) * 2006-12-29 2008-07-10 Honeywell International Inc. Reaction buffer for microarray
US9271653B2 (en) 2007-06-12 2016-03-01 Case Western Reserve University Intra-operative molecular imaging
DE102007029772B4 (de) 2007-06-22 2011-12-08 Aj Innuscreen Gmbh Verfahren und Schnelltest zum Nachweis spezifischer Nukleinsäuresequenzen
IL184478A (en) * 2007-07-08 2017-07-31 Hadasit Medical Res Services & Development Ltd Preparations, methods and kits for identifying carriers of mutations in genes 1brca and 2brca and for the early detection of cancers associated with mutations in these genes
EP2107125A1 (de) 2008-03-31 2009-10-07 Eppendorf Array Technologies SA (EAT) Echtzeit-PCR von Targets auf einem Mikroarray
US20100143901A1 (en) * 2008-12-09 2010-06-10 Roche Molecular Systems, Inc. Nuclease-Free Real-Time Detection of Nucleic Acids
US8691504B2 (en) * 2009-05-26 2014-04-08 Xiamen University Method for detecting variations in nucleic acid sequences
EP2488548A1 (de) 2009-10-14 2012-08-22 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung Lrp1-spleissvariante als krebsmarker
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
AU2011230496B2 (en) 2010-03-26 2015-09-17 Integrated Dna Technologies, Inc. Methods for enhancing nucleic acid hybridization
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
DE102010003781B4 (de) 2010-04-08 2012-08-16 Aj Innuscreen Gmbh Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen
DE102010003782B4 (de) 2010-04-08 2023-09-28 Ist Innuscreen Gmbh Vorrichtung zum Nachweis von Nukleinsäuren
EP3301195B1 (de) 2010-07-12 2022-11-02 Gen-Probe Incorporated Zusammensetzungen und assays zur detektion der 2009 h1n1-influenza-a-virus-nukleinsäure in schweinen
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
DE102010033107A1 (de) 2010-08-02 2012-02-02 Aj Innuscreen Gmbh Nachweis spezifischer Nukleinsäuresequenzen mittels Fluoreszenzlöschung
WO2012016936A1 (de) 2010-07-31 2012-02-09 Aj Innuscreen Gmbh Nachweis spezifischer nukleinsäuresequenzen mittels fluoreszenz - quenching
CA2809457C (en) 2010-09-07 2019-07-30 Integrated Dna Technologies, Inc. Modifications for antisense compounds
WO2012034130A2 (en) 2010-09-10 2012-03-15 Qiagen Methods and compositions for nucleic acid detection
CA2812512A1 (en) 2010-09-29 2012-04-05 Mor Research Applications Ltd. Prognostic methods, compositions and kits for prediction of acute lymphoblastic leukemia (all) relapse
CN103403533B (zh) 2011-02-24 2017-02-15 简.探针公司 用于分辨光信号检测器中不同调制频率的光信号的系统和方法
DE102011078342A1 (de) 2011-06-29 2013-01-03 Aj Innuscreen Gmbh Rehybridisierendes Sondensystem zur qualitativen und quantitativen Messung von spezifischen Nukleinsäuren in Echtzeit
WO2013030841A1 (en) 2011-09-04 2013-03-07 Yissum Research Development Company Of The Hebrew Universitiy Of Jerusalem Ltd. Prognostic methods and compositions for predicting interferon treatment eficacy in a subject
BR112014019168B1 (pt) 2012-02-03 2021-10-05 California Institute Of Technology Método capaz de detectar de forma não degenerada presença ou ausência de analitos em um único volume de amostra e método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos
EP2820157B1 (de) 2012-03-02 2019-05-01 Winthrop-University Hospital Verfahren zur verwendung von sondenbasierter pcr-detektion zum messen der konzentration von dna aus zirkulierenden entmethylierten beta-zellen als massstab für beta-zell-verlust bei diabetes
US9133509B2 (en) 2012-03-22 2015-09-15 Lgc Genomics Limited Polymerase chain reaction detection system
US20150119447A1 (en) 2012-05-09 2015-04-30 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Clustered single nucleotide polymorphisms in the human acetylcholinesterase gene and uses thereof in diagnosis and therapy
CN104662172B (zh) 2012-08-03 2018-07-06 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
EP2712790B1 (de) 2012-09-26 2015-11-18 3A Composites GmbH Gewölbtes Fahrzeugdach mit Verstärkungs- und Dämpfungselement
CN103114131B (zh) 2012-11-30 2018-10-02 珠海市坤元农业科技有限公司 一种引物中部序列干扰pcr技术
AU2013202805B2 (en) 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
US20160265058A1 (en) 2013-11-01 2016-09-15 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Diagnostic methods and kits for determining a personalized treatment regimen for a subject suffering from a pathologic disorder
KR102324117B1 (ko) 2013-11-22 2021-11-10 오리온 디아그노스티카 오와이 가닥 침입에 기초한 증폭에 의한 핵산 검출
CL2015000980A1 (es) 2014-04-18 2016-07-01 Upstartdna Co Ltd Standard Entity Métodos para detectar patógenos en peces de agua fría.
GB201410022D0 (en) 2014-06-05 2014-07-16 Orion Diagnostica Oy Method
US20170252364A1 (en) 2014-07-30 2017-09-07 Mor Research Applications Ltd. Compositions for treatment of acute lymphoblastic leukemia and methods of use thereof
US11091810B2 (en) * 2015-01-27 2021-08-17 BioSpyder Technologies, Inc. Focal gene expression profiling of stained FFPE tissues with spatial correlation to morphology
US10683534B2 (en) * 2015-01-27 2020-06-16 BioSpyder Technologies, Inc. Ligation assays in liquid phase
US9856521B2 (en) * 2015-01-27 2018-01-02 BioSpyder Technologies, Inc. Ligation assays in liquid phase
EP3359690A4 (de) 2015-10-09 2019-06-26 Genefron Ltd. Verfahren und kits zur vorhersage und diagnose der kongenitalen übertragung des humanen cytomegalovirus (hcmv)
US10066263B2 (en) 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
JP6853523B2 (ja) * 2016-07-27 2021-03-31 国立大学法人京都大学 ヘリカーゼを用いたｐｃｒ
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
WO2020069656A1 (zh) 2018-10-05 2020-04-09 福又达生物科技股份有限公司 鱼病原体检测方法
IT202100009338A1 (it) 2021-04-14 2022-10-14 Celera Digital S R L Sistema di interconnessione e predizione aziendale e metodo di funzionamento di sistema di segnalazione
DK181862B1 (en) 2022-03-21 2025-02-24 Samplix Aps Method and kit for targeted enrichment of large dna molecules for long-read sequencing using facs or microfluidic partitioning

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4220450A (en) 1978-04-05 1980-09-02 Syva Company Chemically induced fluorescence immunoassay
US4766064A (en) 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay employing polyether and diagnostic kit
CA1273552A (en) * 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
EP0232967B1 (de) 1986-01-10 1993-04-28 Amoco Corporation Kompetitiver homogener Test
US5635347A (en) 1986-04-30 1997-06-03 Igen, Inc. Rapid assays for amplification products
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5273879A (en) * 1987-07-23 1993-12-28 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
WO1990003445A1 (en) 1988-09-30 1990-04-05 The Salk Institute For Biological Studies Target nucleic acid amplification/detection systems
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
WO1992014845A1 (en) * 1991-02-26 1992-09-03 Worcester Foundation For Experimental Biology Diagnosing cystic fibrosis and other genetic diseases using fluorescence resonance energy transfer (fret)
EP0519338B1 (de) * 1991-06-20 1996-08-28 F. Hoffmann-La Roche Ag Verbesserte Methoden zur Nukleinsäure-Amplifizierung
AU645915B2 (en) 1991-07-23 1994-01-27 F. Hoffmann-La Roche Ag Improvements in the in situ PCR
DK0549107T3 (da) 1991-10-11 1998-08-31 Dade Behring Marburg Gmbh Fremgangsmåde til fremstilling af et polynukleotidtil anvendelse ved enkeltprimeramplifikation og phosphorthioatholdige oli
JP2788786B2 (ja) * 1991-11-15 1998-08-20 イゲン，インコーポレーテッド 増幅生成物の迅速アッセイ
US5332659A (en) 1992-04-09 1994-07-26 The United States Of America As Represented By The Secretary Of The Navy Light emission-or absorbance-based binding assays for polynucleic acids
US6020124A (en) 1992-04-27 2000-02-01 Trustees Of Dartmouth College Detection of soluble gene sequences in biological fluids
GB9211979D0 (en) 1992-06-05 1992-07-15 Buchard Ole Uses of nucleic acid analogues
US5849544A (en) 1992-07-24 1998-12-15 University Of Australia Amplification and detection process
KR100316498B1 (ko) * 1992-08-04 2002-06-20 다니엘 엘. 캐시앙, 헨리 엘. 노르호프, 피터 알. 쉬어리 핵산서열증폭방법
EP0599388B1 (de) 1992-11-20 2000-08-02 Koninklijke Philips Electronics N.V. Halbleitervorrichtung mit einem programmierbaren Element
JPH06153997A (ja) * 1992-11-27 1994-06-03 Canon Inc 検出信号増幅による標的核酸の検出方法
EP0601889A2 (de) * 1992-12-10 1994-06-15 Maine Medical Center Research Institute Nukleinsäure-Sonden
US5364790A (en) 1993-02-16 1994-11-15 The Perkin-Elmer Corporation In situ PCR amplification system
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
GB9311386D0 (en) 1993-06-02 1993-07-21 Pna Diagnostics As Nucleic acid analogue assay procedures
DE69422678T2 (de) * 1993-10-12 2001-02-22 Matsushita Electric Industrial Co., Ltd. Verschlüsselungssystem, Verschlüsselungsgerät und Entschlüsselungsgerät
EP2423322A1 (de) 1993-11-12 2012-02-29 PHRI Properties, Inc. Hybridisierungsprobe zur Nukleinsäuredetektion, universelle Stammstrukturen, Methoden und Reagentiensätze
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5629178A (en) 1994-10-28 1997-05-13 Genetics & Ivf Institute Method for enhancing amplification in the polymerase chain reaction employing peptide nucleic acid (PNA)
IE72524B1 (en) 1994-11-04 1997-04-23 Elan Med Tech Analyte-controlled liquid delivery device and analyte monitor
ATE196322T1 (de) 1995-05-05 2000-09-15 Perkin Elmer Corp Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
WO1997007235A2 (en) 1995-08-14 1997-02-27 Abbott Laboratories All-in-one nucleic acid amplification assay

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