ATE286144T1 - VERWENDUNG VON FLUORESZIERENDEN ßMOLECULAR BEACONSß ZUM NACHWEIS VON METHYLIERTEN NUKLEINSÄUREN - Google Patents

VERWENDUNG VON FLUORESZIERENDEN ßMOLECULAR BEACONSß ZUM NACHWEIS VON METHYLIERTEN NUKLEINSÄUREN

Info

Publication number
ATE286144T1
ATE286144T1 AT00904696T AT00904696T ATE286144T1 AT E286144 T1 ATE286144 T1 AT E286144T1 AT 00904696 T AT00904696 T AT 00904696T AT 00904696 T AT00904696 T AT 00904696T AT E286144 T1 ATE286144 T1 AT E286144T1
Authority
AT
Austria
Prior art keywords
nucleic acid
stem
region
nucleotides
nucleic acids
Prior art date
Application number
AT00904696T
Other languages
English (en)
Inventor
Peter H Kay
Original Assignee
Epigenomics Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Ag filed Critical Epigenomics Ag
Application granted granted Critical
Publication of ATE286144T1 publication Critical patent/ATE286144T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
AT00904696T 1999-02-01 2000-02-01 VERWENDUNG VON FLUORESZIERENDEN ßMOLECULAR BEACONSß ZUM NACHWEIS VON METHYLIERTEN NUKLEINSÄUREN ATE286144T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPP8448A AUPP844899A0 (en) 1999-02-01 1999-02-01 A method for detecting methylated nucleic acids
PCT/AU2000/000053 WO2000046398A1 (en) 1999-02-01 2000-02-01 Use of fluorescent molecular beacons in the detection of methylated nucleic acids

Publications (1)

Publication Number Publication Date
ATE286144T1 true ATE286144T1 (de) 2005-01-15

Family

ID=3812648

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00904696T ATE286144T1 (de) 1999-02-01 2000-02-01 VERWENDUNG VON FLUORESZIERENDEN ßMOLECULAR BEACONSß ZUM NACHWEIS VON METHYLIERTEN NUKLEINSÄUREN

Country Status (9)

Country Link
US (2) US20030017465A1 (de)
EP (1) EP1151138B1 (de)
JP (1) JP2002535998A (de)
AT (1) ATE286144T1 (de)
AU (1) AUPP844899A0 (de)
CA (1) CA2361654A1 (de)
DE (1) DE60017089T2 (de)
ES (1) ES2235832T3 (de)
WO (1) WO2000046398A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6960436B2 (en) * 2002-02-06 2005-11-01 Epigenomics Ag Quantitative methylation detection in DNA samples
DE10304219B3 (de) * 2003-01-30 2004-08-19 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität
CN1784590B (zh) * 2003-03-07 2010-11-24 新泽西医学与牙科大学 可光学解码的微载体,阵列和方法
DE10338308B4 (de) * 2003-08-15 2006-10-19 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in DNA
KR100885177B1 (ko) * 2004-04-12 2009-02-23 학교법인 포항공과대학교 표적 ｄｎａ 또는 ｒｎａ 탐지용 올리고뉴클레오티드
GB0714058D0 (en) * 2007-07-19 2007-08-29 Abertec Ltd detection of methylation in nucleic acid sequences
US9393566B2 (en) * 2008-06-23 2016-07-19 Canon U.S. Life Sciences, Inc. System and method for temperature referencing for melt curve data collection
CN102449167B (zh) * 2009-05-26 2014-12-17 厦门大学 一种检测核酸序列变异的方法
CN102051408B (zh) * 2009-11-04 2012-07-04 高新 用于前列腺癌转移早期诊断的试剂盒
EP2865764B1 (de) 2012-06-20 2018-09-05 Toray Industries, Inc. Verfahren zum nachweis von nukleinsäure und verwendung eines kits zum nachweis von nukleinsäure
GB201402644D0 (en) 2014-02-14 2014-04-02 Base4 Innovation Ltd Methylation detection method
KR20210026867A (ko) * 2019-09-02 2021-03-10 주식회사 시선바이오머티리얼스 신규한 pna 올리고머, 이의 dna 메틸화 검출용도 및 이를 이용한 dna 메틸화 검출방법
CN113552191B (zh) * 2021-07-28 2023-11-21 江苏师范大学 基于多层dna放大回路检测甲基化dna的比例型电化学传感器的构建方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5426180A (en) * 1991-03-27 1995-06-20 Research Corporation Technologies, Inc. Methods of making single-stranded circular oligonucleotides
US5808036A (en) * 1993-09-01 1998-09-15 Research Corporation Technologies Inc. Stem-loop oligonucleotides containing parallel and antiparallel binding domains
JP3898228B2 (ja) * 1996-04-12 2007-03-28 ザ パブリック ヘルス リサーチ インスティチュート オブ ザ シティー オブ ニューヨーク インク 検出プローブ、キット及びアッセイ
US6017704A (en) * 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
AU7847798A (en) * 1996-12-13 1998-07-03 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Fluorescent nucleotide analog hairpin formation for detection of nuclei c acid hybridization
WO1999022018A2 (en) * 1997-10-27 1999-05-06 Boston Probes, Inc. Methods, kits and compositions pertaining to pna molecular beacons
GB9725237D0 (en) * 1997-11-29 1998-01-28 Secr Defence Amplification system
CA2349127A1 (en) * 1998-10-06 2000-04-13 Emory University Molecular diagnostics for galactosemia

Also Published As

Publication number Publication date
JP2002535998A (ja) 2002-10-29
DE60017089T2 (de) 2006-01-05
EP1151138B1 (de) 2004-12-29
US20050221329A1 (en) 2005-10-06
EP1151138A1 (de) 2001-11-07
ES2235832T3 (es) 2005-07-16
WO2000046398A1 (en) 2000-08-10
US20030017465A1 (en) 2003-01-23
AUPP844899A0 (en) 1999-02-25
EP1151138A4 (de) 2003-01-22
CA2361654A1 (en) 2000-08-10
DE60017089D1 (de) 2005-02-03

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