ES2722900T3 - Portaobjetos para micromatrices y método para detectar algas tóxicas - Google Patents

Portaobjetos para micromatrices y método para detectar algas tóxicas Download PDF

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Publication number
ES2722900T3
ES2722900T3 ES13773308T ES13773308T ES2722900T3 ES 2722900 T3 ES2722900 T3 ES 2722900T3 ES 13773308 T ES13773308 T ES 13773308T ES 13773308 T ES13773308 T ES 13773308T ES 2722900 T3 ES2722900 T3 ES 2722900T3
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seq
organism
taxonomic
sequences given
selectively detecting
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English (en)
Inventor
Linda Medlin
Marina Montresor
Edna Graneli
Beatriz Reguera
Robin Raine
Bente Edvardsen
Jane Lewis
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Microbia Environnement
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Microbia Environnement
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un sistema de matriz que comprende un portaobjetos para micromatrices configurado para detectar simultáneamente una diversidad de organismos en una muestra, en donde el portaobjetos para micromatrices comprende sondas de ácido nucleico que tienen fragmentos de secuencia de ARN 18S o 28S únicos para cada organismo o grupo taxonómico del mismo, en donde la pluralidad de organismos comprende organismos de algas tóxicas y en donde algunas de las sondas de ácido nucleico son específicas para la detección de la especie de cada organismo a detectar y otras sondas de ácido nucleico son específicas para la detección de al menos un taxón de nivel superior al que pertenece cada organismo a detectar, en donde el portaobjetos para micromatrices comprende: a. al menos una sonda seleccionada entre el grupo que consiste en controles positivos y negativos, que tiene una secuencia seleccionada entre la SEC ID NO: 1 a la SEC ID NO: 4, la SEC ID NO: 7 y la SEC ID NO: 8; b. al menos las dos sondas capaces de detectar selectivamente al menos uno de los microorganismos eucarióticos, que tienen secuencias dadas respectivamente en la SEC ID NO: 9 a la SEC ID NO: 10; c. al menos todas las sondas capaces de detectar selectivamente el filum taxonómico del organismo, que tiene secuencias dadas respectivamente en la SEC ID NO: 11 a la SEC ID NO: 13; d. al menos todas las sondas capaces de detectar selectivamente la clase taxonómica del organismo, que tienen secuencias dadas respectivamente en la SEC ID NO: 14 a la SEC ID NO: 16; e. al menos todas las sondas capaces de detectar selectivamente un clado taxonómico del organismo, a un nivel taxonómico intermedio entre clase y género, que tienen secuencias dadas respectivamente en SEC ID NO: 17 a SEC ID NO: 41; f. al menos todas las sondas capaces de detectar selectivamente el género taxonómico del organismo, que tienen secuencias dadas respectivamente en la SEC ID NO: 42 a la SEC ID NO: 69; g. al menos todas las sondas capaces de detectar selectivamente la especie taxonómica del organismo, que tienen secuencias dadas respectivamente en la SEC ID NO: 70 a la SEC ID NO: 252, y h. al menos todas las sondas capaces de detectar selectivamente la cepa taxonómica del organismo, en donde existen cepas tanto tóxicas como no tóxicas de la misma especie, que tienen secuencias dadas respectivamente en la SEC ID NO: 70 a la SEC ID NO: 252.
ES13773308T 2013-07-19 2013-07-19 Portaobjetos para micromatrices y método para detectar algas tóxicas Active ES2722900T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/GB2013/051938 WO2015008011A1 (en) 2013-07-19 2013-07-19 Microarray slide and method for detecting toxic algae

Publications (1)

Publication Number Publication Date
ES2722900T3 true ES2722900T3 (es) 2019-08-19

Family

ID=49303996

Family Applications (1)

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ES13773308T Active ES2722900T3 (es) 2013-07-19 2013-07-19 Portaobjetos para micromatrices y método para detectar algas tóxicas

Country Status (5)

Country Link
US (1) US10294533B2 (es)
EP (1) EP3022317B1 (es)
ES (1) ES2722900T3 (es)
PT (1) PT3022317T (es)
WO (1) WO2015008011A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3141613A1 (en) * 2015-09-11 2017-03-15 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Detection of quality of surface water
US10890573B2 (en) 2017-12-19 2021-01-12 International Business Machines Corporation Facile methods to detect toxin in seafood
FR3085965A1 (fr) 2018-09-17 2020-03-20 Microbia Environnement Utilisation de sondes pour la detection d'algues toxiques, procede de detection et kits correspondants
CN113393131B (zh) * 2021-06-18 2024-01-30 南京大学 一种基于环境dna宏条形码技术的浮游植物完整性评价方法
CN113652472B (zh) * 2021-07-27 2022-07-26 壹健生物科技(苏州)有限公司 一种检测产毒微囊藻菌型的探针组合、芯片、试剂盒及方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002365088A1 (en) 2001-10-30 2003-07-09 University Of Delaware Rapid high-throughput screen for detecting biological species in ballast water

Also Published As

Publication number Publication date
US20160281178A1 (en) 2016-09-29
WO2015008011A1 (en) 2015-01-22
US10294533B2 (en) 2019-05-21
PT3022317T (pt) 2019-05-20
EP3022317B1 (en) 2019-02-06
EP3022317A1 (en) 2016-05-25

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