AR103576A1 - Molécula sustrato - Google Patents

Molécula sustrato

Info

Publication number
AR103576A1
AR103576A1 ARP160100275A ARP160100275A AR103576A1 AR 103576 A1 AR103576 A1 AR 103576A1 AR P160100275 A ARP160100275 A AR P160100275A AR P160100275 A ARP160100275 A AR P160100275A AR 103576 A1 AR103576 A1 AR 103576A1
Authority
AR
Argentina
Prior art keywords
substrate molecule
polymerase
cutting enzyme
fluorophore
releasing
Prior art date
Application number
ARP160100275A
Other languages
English (en)
Inventor
A Judice Stephen
Original Assignee
Envirologix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Envirologix Inc filed Critical Envirologix Inc
Publication of AR103576A1 publication Critical patent/AR103576A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)

Abstract

Composiciones y métodos para ensayar la actividad de enzima de corte y polimerasa en una reacción mediante el uso de una molécula sustrato de ácido nucleico que detecta actividades de enzima de Corte y extensión de polimerasa mediante la liberación de un reportero detectable (por ejemplo, un fluoróforo).
ARP160100275A 2015-01-30 2016-01-29 Molécula sustrato AR103576A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562110237P 2015-01-30 2015-01-30

Publications (1)

Publication Number Publication Date
AR103576A1 true AR103576A1 (es) 2017-05-17

Family

ID=56544204

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP160100275A AR103576A1 (es) 2015-01-30 2016-01-29 Molécula sustrato

Country Status (10)

Country Link
US (2) US10570441B2 (es)
EP (1) EP3250715A4 (es)
JP (1) JP7004570B2 (es)
CN (1) CN107532206B (es)
AR (1) AR103576A1 (es)
BR (1) BR112017016350A2 (es)
CA (1) CA2975328A1 (es)
MX (1) MX2017009833A (es)
RU (1) RU2755495C2 (es)
WO (1) WO2016123029A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112017016350A2 (pt) 2015-01-30 2018-03-27 Envirologix Inc. molécula de substrato
WO2018045141A1 (en) * 2016-09-02 2018-03-08 New England Biolabs, Inc. Analysis of chromatin using a nicking enzyme
CN112204385A (zh) * 2018-06-29 2021-01-08 富士胶片株式会社 缺陷显示装置及方法

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6090555A (en) 1997-12-11 2000-07-18 Affymetrix, Inc. Scanned image alignment systems and methods
US5571639A (en) 1994-05-24 1996-11-05 Affymax Technologies N.V. Computer-aided engineering system for design of sequence arrays and lithographic masks
US5795716A (en) 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
US5733729A (en) 1995-09-14 1998-03-31 Affymetrix, Inc. Computer-aided probability base calling for arrays of nucleic acid probes on chips
US6586806B1 (en) 1997-06-20 2003-07-01 Cypress Semiconductor Corporation Method and structure for a single-sided non-self-aligned transistor
DE69823206T2 (de) 1997-07-25 2004-08-19 Affymetrix, Inc. (a Delaware Corp.), Santa Clara Verfahren zur herstellung einer bio-informatik-datenbank
WO1999009218A1 (en) 1997-08-15 1999-02-25 Affymetrix, Inc. Polymorphism detection utilizing clustering analysis
JP3565025B2 (ja) 1998-07-07 2004-09-15 日産自動車株式会社 治具交換装置および治具交換方法
US6185561B1 (en) 1998-09-17 2001-02-06 Affymetrix, Inc. Method and apparatus for providing and expression data mining database
US20020183936A1 (en) 2001-01-24 2002-12-05 Affymetrix, Inc. Method, system, and computer software for providing a genomic web portal
WO2003008624A2 (en) 2001-07-15 2003-01-30 Keck Graduate Institute Nucleic acid amplification using nicking agents
US6585606B2 (en) 2001-07-16 2003-07-01 Thomas S. Penrose Golf club accessory
JP4632788B2 (ja) 2002-09-20 2011-02-16 ニュー・イングランド・バイオラブズ・インコーポレイティッド 核酸のヘリカーゼ依存性増幅
WO2006088911A2 (en) 2005-02-15 2006-08-24 Anteon Corporation Sequence-specific detection of nucleotide sequences
CN100489112C (zh) * 2006-03-10 2009-05-20 杭州优思达生物技术有限公司 切口酶扩增靶核酸序列的方法及用于扩增靶核酸序列的试剂盒及其应用
US9689031B2 (en) * 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
CA2755615A1 (en) * 2009-03-18 2010-09-23 Sequenom, Inc. Use of thermostable endonucleases for generating reporter molecules
US9150919B2 (en) 2009-04-16 2015-10-06 Padma Arunachalam Methods and compositions to detect and differentiate small RNAs in RNA maturation pathway
CA2810856C (en) * 2010-08-13 2022-05-17 Envirologix Inc. Compositions and methods for quantifying a nucleic acid sequence in a sample
WO2013040992A1 (zh) 2011-09-20 2013-03-28 苏州瑞博生物技术有限公司 一种双链核酸及其在核糖核酸酶检测中的应用和试剂盒
IN2014DN08831A (es) * 2012-04-09 2015-05-22 Envirologix Inc
WO2014161975A1 (en) 2013-04-05 2014-10-09 Qiagen Gmbh Method for performing a melting curve analysis
BR112017016350A2 (pt) 2015-01-30 2018-03-27 Envirologix Inc. molécula de substrato

Also Published As

Publication number Publication date
EP3250715A1 (en) 2017-12-06
EP3250715A4 (en) 2018-10-24
JP2018503390A (ja) 2018-02-08
RU2755495C2 (ru) 2021-09-16
US20200157612A1 (en) 2020-05-21
RU2017130477A (ru) 2019-02-28
WO2016123029A1 (en) 2016-08-04
MX2017009833A (es) 2017-11-02
US10570441B2 (en) 2020-02-25
BR112017016350A2 (pt) 2018-03-27
JP7004570B2 (ja) 2022-02-10
RU2017130477A3 (es) 2019-08-07
CN107532206B (zh) 2022-08-05
US11220708B2 (en) 2022-01-11
CA2975328A1 (en) 2016-08-04
US20180037940A1 (en) 2018-02-08
CN107532206A (zh) 2018-01-02

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