WO2014101276A3 - 确定核酸样本的两个预定位点是否具有已知突变的方法、试剂盒以及引物 - Google Patents

确定核酸样本的两个预定位点是否具有已知突变的方法、试剂盒以及引物 Download PDF

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WO2014101276A3
WO2014101276A3 PCT/CN2013/001453 CN2013001453W WO2014101276A3 WO 2014101276 A3 WO2014101276 A3 WO 2014101276A3 CN 2013001453 W CN2013001453 W CN 2013001453W WO 2014101276 A3 WO2014101276 A3 WO 2014101276A3
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nucleic acid
predetermined sites
acid sample
primer
known mutations
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PCT/CN2013/001453
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English (en)
French (fr)
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WO2014101276A2 (zh
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许华曦
卜国军
张含
张云武
刘家珍
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厦门人瑞生物医药科技有限公司
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Publication of WO2014101276A2 publication Critical patent/WO2014101276A2/zh
Publication of WO2014101276A3 publication Critical patent/WO2014101276A3/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

本发明公开了确定核酸样本的两个预定位点是否具有已知突变的方法、试剂盒以及引物。其中，该方法包括：利用第一引物和第二引物对核酸样本进行PCR扩增，并且在PCR扩增体系中加入核酸探针，核酸探针对应两个预定位点之间至少一部分对应的核酸序列；检测反应体系的发光，以便确定核酸样本的预定位点是否存在已知突变，第一引物的近3'端包含与两个预定位点之一已知突变对应的碱基，第二引物的近3'端包含与另一个已知突变对应的碱基，核酸探针具有：发光基团，发光基团形成于核酸探针的5'端，调节基团，调节基团形成于核酸探针的3'端，并且调节基团可以调节发光基团的发光。利用该方法能够有效地确定核酸样本的两个预定位点是否具有已知突变。
PCT/CN2013/001453 2012-12-31 2013-11-27 确定核酸样本的两个预定位点是否具有已知突变的方法、试剂盒以及引物 WO2014101276A2 (zh)

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CN201210592416.8 2012-12-31
CN2012105924168A CN103103259A (zh) 2012-12-31 2012-12-31 确定核酸样本的两个预定位点是否具有已知突变的方法、试剂盒以及引物

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WO2014101276A2 WO2014101276A2 (zh) 2014-07-03
WO2014101276A3 true WO2014101276A3 (zh) 2014-08-28

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CN103103259A (zh) * 2012-12-31 2013-05-15 厦门人瑞生物医药科技有限公司 确定核酸样本的两个预定位点是否具有已知突变的方法、试剂盒以及引物
CN106011298A (zh) * 2015-11-12 2016-10-12 厦门人瑞生物医药科技有限公司 一种ApoE试剂盒、引物及其用途
CN110114458A (zh) * 2016-09-22 2019-08-09 豪夫迈·罗氏有限公司 Pol6聚合酶变体
CN109097457A (zh) * 2017-06-20 2018-12-28 深圳华大智造科技有限公司 确定核酸样本中预定位点突变类型的方法
CN110029159A (zh) * 2019-02-26 2019-07-19 成都华青精准医疗科技有限公司 一种用于ApoE基因分型检测的核苷酸序列组及其应用

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CN103103259A (zh) * 2012-12-31 2013-05-15 厦门人瑞生物医药科技有限公司 确定核酸样本的两个预定位点是否具有已知突变的方法、试剂盒以及引物

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CN101354338A (zh) * 2007-07-27 2009-01-28 上海主健生物工程有限公司 检测心脑疾病遗传易感性的试剂盒
CN102586404B (zh) * 2011-01-07 2013-10-16 同济大学 一种特异检测ApoE4基因型的试剂盒及其应用

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Publication number Priority date Publication date Assignee Title
CN103103259A (zh) * 2012-12-31 2013-05-15 厦门人瑞生物医药科技有限公司 确定核酸样本的两个预定位点是否具有已知突变的方法、试剂盒以及引物

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CALERO, O. ET AL.: "Apolipoprotein E genotyping method by Real Time PCR, a fast and cost-effective alternative to the TaqMan® and FRET assays", JOURNAL OF NEUROSCIENCE METHODS, vol. 2, no. 183, 15 October 2009 (2009-10-15), pages 238 - 240 *

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CN103103259A (zh) 2013-05-15

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