WO2014102761A8 - Cebadores para detección y tipificación de cepas bacterianas productoras de carbapenemasas, kit y método de detección - Google Patents
Cebadores para detección y tipificación de cepas bacterianas productoras de carbapenemasas, kit y método de detección Download PDFInfo
- Publication number
- WO2014102761A8 WO2014102761A8 PCT/IB2014/000230 IB2014000230W WO2014102761A8 WO 2014102761 A8 WO2014102761 A8 WO 2014102761A8 IB 2014000230 W IB2014000230 W IB 2014000230W WO 2014102761 A8 WO2014102761 A8 WO 2014102761A8
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- detection
- carbapenemase
- primers
- bacterial strains
- kit
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Se proponen unas parejas de cebadores, su uso para detección de genes de carbapenemasas y cepas bacterianas productoras de carbapenemasas, y un método de diagnóstico empleando dicho conjunto de cebadores. Se propone además, preferiblemente, el uso de un grupo de sondas dentro de cada reacción de amplificación, más preferiblemente PCR multiplex, para la identificación selectiva y detección de genes de carbapenemasas a partir de una muestra biológica. Los cebadores objeto de esta invención pueden ser utilizados para la preparación de un kit para identificación y detección de genes de carbapenemasas, preferiblemente de genes de carbapenemasas contenidos en cepas bacterianas productoras de dichas enzimas.
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP18168826.8A EP3392350A1 (en) | 2012-12-31 | 2014-02-28 | Primers for detection and typing of carbapenemase producing bacterial strains, and detection method and kit |
US14/758,458 US9914980B2 (en) | 2012-12-31 | 2014-02-28 | Primers for the detection and typing of carbapenemase-producing bacterial strains, and detection method and kit |
EP18168831.8A EP3392346A1 (en) | 2012-12-31 | 2014-02-28 | Primers for detection and typing of carbapenemase producing bacterial strains, and detection method and kit |
EP14718156.4A EP2940152B1 (en) | 2012-12-31 | 2014-02-28 | Primers for the detection and typing of carbapenemase-producing bacterial strains, and detection method and kit |
ES14718156.4T ES2689308T3 (es) | 2012-12-31 | 2014-02-28 | Cebadores para detección y tipado de cepas bacterianas productoras de carbapenemasas, método y kit de detección |
US15/877,834 US20180179583A1 (en) | 2012-12-31 | 2018-01-23 | Primers for the detection and typing of carbapenemase-producing bacterial strains and detection method and kit |
US15/877,851 US20180230520A1 (en) | 2012-12-31 | 2018-01-23 | Primers for the detection and typing of carbapenemase-producing bacterial strains and detection method and kit |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ESP2010232077 | 2012-12-31 | ||
ESP2012232077 | 2012-12-31 | ||
ES201232077 | 2012-12-31 |
Related Child Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US14/758,458 A-371-Of-International US9914980B2 (en) | 2012-12-31 | 2014-02-28 | Primers for the detection and typing of carbapenemase-producing bacterial strains, and detection method and kit |
US15/877,834 Division US20180179583A1 (en) | 2012-12-31 | 2018-01-23 | Primers for the detection and typing of carbapenemase-producing bacterial strains and detection method and kit |
US15/877,851 Division US20180230520A1 (en) | 2012-12-31 | 2018-01-23 | Primers for the detection and typing of carbapenemase-producing bacterial strains and detection method and kit |
Publications (3)
Publication Number | Publication Date |
---|---|
WO2014102761A2 WO2014102761A2 (es) | 2014-07-03 |
WO2014102761A8 true WO2014102761A8 (es) | 2014-08-28 |
WO2014102761A3 WO2014102761A3 (es) | 2015-03-12 |
Family
ID=50513378
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2014/000230 WO2014102761A2 (es) | 2012-12-31 | 2014-02-28 | Cebadores para detección y tipificación de cepas bacterianas productoras de carbapenemasas, kit y método de detección |
Country Status (4)
Country | Link |
---|---|
US (3) | US9914980B2 (es) |
EP (3) | EP3392350A1 (es) |
ES (1) | ES2689308T3 (es) |
WO (1) | WO2014102761A2 (es) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105671148A (zh) * | 2016-01-15 | 2016-06-15 | 武汉艾米森生命科技有限公司 | 用于检测耐碳青霉烯类肠杆菌试剂盒与检测方法 |
CN107460242B (zh) * | 2017-08-15 | 2022-06-10 | 北京紫萌医药科技有限公司 | 一种能同时检测多种高耐药基因的检测试剂盒及其应用 |
CN107828856B (zh) * | 2017-11-23 | 2020-05-26 | 中国人民解放军南京军区南京总医院 | 一种pcr-lf技术检测碳青霉烯类耐药基因kpc和ndm的引物组合物及其应用 |
CN110904199A (zh) * | 2019-11-22 | 2020-03-24 | 南方医科大学南方医院 | 检测碳青霉烯酶blaKPC基因的引物、方法及试剂盒 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1246474C (zh) | 2001-07-13 | 2006-03-22 | 刘元 | 采用基因芯片技术检测耐药基因 |
US7842800B2 (en) * | 2004-04-02 | 2010-11-30 | Rosetta Genomics Ltd. | Bioinformatically detectable group of novel regulatory bacterial and bacterial associated oligonucleotides and uses thereof |
MX2008014795A (es) * | 2006-05-25 | 2009-02-11 | Univ Creighton | Cebadores para uso en la deteccion de metalo-beta-lactamasas. |
KR100868765B1 (ko) * | 2006-09-29 | 2008-11-17 | 삼성전자주식회사 | 항생제 내성 박테리아의 표적 서열을 증폭시킬 수 있는프라이머 세트, 박테리아의 표적 서열에 특이적으로혼성화하는 프로브 세트, 상기 프로브 세트가 고정화되어있는 마이크로어레이 및 상기 프로브 세트를 이용하여박테리아의 존재를 검출하는 방법 |
CA2682299C (en) | 2007-04-06 | 2015-12-22 | Becton, Dickinson And Company | Compositions and methods for the identification of a carbapenemase gene |
FI20095544A0 (fi) * | 2009-05-15 | 2009-05-15 | Juha Kirveskari | Menetelmä ja kitti antibioottiresistenttien bakteerien tunnistusta varten |
RU2415932C1 (ru) | 2009-10-23 | 2011-04-10 | Закрытое акционерное общество "Научно-производственное предприятие ИММУНОТЕХ" (ЗАО "НПП ИММУНОТЕХ") | Способ идентификации генов бета-лактамаз расширенного спектра действия класса а |
WO2011138402A1 (en) | 2010-05-05 | 2011-11-10 | Check-Points Holding B.V. | Assays, compositions and methods for detecting drug resistant micro-organisms |
EP2694685B1 (en) * | 2011-04-08 | 2019-01-09 | Azzazy, Hassan, Mohamed, Ei-said | Detection of nucleic acids using unmodified gold nanoparticles |
CN102603875B (zh) * | 2011-12-26 | 2014-07-09 | 台州市立医院 | 鲍曼复合醋酸钙不动杆菌oxa-23 突变基因的荧光定量pcr 试剂盒 |
-
2014
- 2014-02-28 ES ES14718156.4T patent/ES2689308T3/es active Active
- 2014-02-28 US US14/758,458 patent/US9914980B2/en active Active
- 2014-02-28 EP EP18168826.8A patent/EP3392350A1/en not_active Withdrawn
- 2014-02-28 EP EP18168831.8A patent/EP3392346A1/en not_active Withdrawn
- 2014-02-28 WO PCT/IB2014/000230 patent/WO2014102761A2/es active Application Filing
- 2014-02-28 EP EP14718156.4A patent/EP2940152B1/en active Active
-
2018
- 2018-01-23 US US15/877,834 patent/US20180179583A1/en not_active Abandoned
- 2018-01-23 US US15/877,851 patent/US20180230520A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP3392350A1 (en) | 2018-10-24 |
WO2014102761A2 (es) | 2014-07-03 |
WO2014102761A3 (es) | 2015-03-12 |
US20180179583A1 (en) | 2018-06-28 |
US9914980B2 (en) | 2018-03-13 |
US20160122805A1 (en) | 2016-05-05 |
EP2940152B1 (en) | 2018-06-27 |
US20180230520A1 (en) | 2018-08-16 |
ES2689308T3 (es) | 2018-11-13 |
EP3392346A1 (en) | 2018-10-24 |
EP2940152A2 (en) | 2015-11-04 |
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