DK0550696T3 - Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus - Google Patents

Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus

Info

Publication number
DK0550696T3
DK0550696T3 DK91920000T DK91920000T DK0550696T3 DK 0550696 T3 DK0550696 T3 DK 0550696T3 DK 91920000 T DK91920000 T DK 91920000T DK 91920000 T DK91920000 T DK 91920000T DK 0550696 T3 DK0550696 T3 DK 0550696T3
Authority
DK
Denmark
Prior art keywords
pct
nucleic acid
enzyme
thermosipho africanus
purified thermostable
Prior art date
Application number
DK91920000T
Other languages
English (en)
Inventor
David H Gelfand
Frances C Lawyer
Richard D Abramson
I Lawrence Greenfield
Fred L Reichert
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of DK0550696T3 publication Critical patent/DK0550696T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Beans For Foods Or Fodder (AREA)
  • Feed For Specific Animals (AREA)
  • Saccharide Compounds (AREA)
DK91920000T 1990-09-28 1991-09-26 Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus DK0550696T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US59049090A 1990-09-28 1990-09-28
PCT/US1991/007076 WO1992006202A1 (en) 1990-09-28 1991-09-26 PURIFIED THERMOSTABLE NUCLEIC ACID POLYMERASE ENZYME FROM $i(THERMOSIPHO AFRICANUS)

Publications (1)

Publication Number Publication Date
DK0550696T3 true DK0550696T3 (da) 2001-03-19

Family

ID=24362481

Family Applications (1)

Application Number Title Priority Date Filing Date
DK91920000T DK0550696T3 (da) 1990-09-28 1991-09-26 Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus

Country Status (10)

Country Link
US (1) US5968799A (da)
EP (1) EP0550696B1 (da)
JP (1) JP2582980B2 (da)
AT (1) ATE197718T1 (da)
AU (1) AU658378B2 (da)
CA (1) CA2092317A1 (da)
DE (1) DE69132477T2 (da)
DK (1) DK0550696T3 (da)
ES (1) ES2152923T3 (da)
WO (1) WO1992006202A1 (da)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69132830T2 (de) * 1990-10-05 2002-07-18 Hoffmann La Roche Verfahren und Reagentien für die Identifikation von Bakterien
US5222875A (en) * 1991-05-31 1993-06-29 Praxair Technology, Inc. Variable speed hydraulic pump system for liquid trailer
EP0730642A1 (en) * 1993-11-25 1996-09-11 Pacific Enzymes Limited Improved polymerase
US6100078A (en) * 1994-04-01 2000-08-08 Gen-Probe Incorporated Purified DNA polymerase from bacillus stearothermophilus ATCC 12980
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5939257A (en) * 1994-04-18 1999-08-17 Amersham Life Science, Inc. Thermostable alkaline phosphatases
WO1995030756A1 (en) * 1994-04-18 1995-11-16 United States Biochemical Corporation Thermostable alkaline phosphatase of thermus thermophilus
US5912155A (en) * 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US5948614A (en) * 1995-09-08 1999-09-07 Life Technologies, Inc. Cloned DNA polymerases from thermotoga maritima and mutants thereof
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
US5861295A (en) 1997-01-02 1999-01-19 Life Technologies, Inc. Nucleic acid-free thermostable enzymes and methods of production thereof
US6306588B1 (en) 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
US6670127B2 (en) 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
EP1538206B1 (en) * 1997-09-16 2010-03-24 Centocor, Inc. Method for the complete chemical synthesis and assembly of genes and genomes
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US7179590B2 (en) * 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US20030138782A1 (en) * 2001-01-19 2003-07-24 Evans Glen A. Computer-directed assembly of a polynucleotide encoding a target polypeptide
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US20030180741A1 (en) 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
JP4782668B2 (ja) 2003-03-31 2011-09-28 エフ．ホフマン−ラ ロシュ アーゲー 日本脳炎ウィルス血清グループのメンバーを包含する一定のフラビウィルスを検出するための組成物及び方法
WO2004094986A2 (en) 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
CA2535922A1 (en) * 2003-09-23 2005-04-07 University Of Missouri Methods of synthesizing polynucleotides using thermostable enzymes
EP1697534B1 (en) 2003-12-01 2010-06-02 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
US7645575B2 (en) 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
US7993832B2 (en) 2006-08-14 2011-08-09 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
WO2008140484A2 (en) 2006-11-09 2008-11-20 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
US8206929B2 (en) 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
CA2831180C (en) 2011-04-11 2017-02-14 Keith Bauer Dna polymerases with improved activity
WO2012146260A1 (de) 2011-04-23 2012-11-01 Biolytix Ag Herstellung und verwendung von proteinen in der molekularbiologie
EP3461807B1 (en) 2011-06-08 2023-07-12 Life Technologies Corporation Design and development of novel detergents for use in pcr systems
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
WO2013083264A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
WO2013083262A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
EP2788479B1 (en) 2011-12-08 2016-02-10 Roche Diagnostics GmbH Dna polymerases with improved activity
US9914964B2 (en) 2013-10-25 2018-03-13 Life Technologies Corporation Compounds for use in PCR systems and applications thereof
CN112760305B (zh) * 2021-01-25 2022-04-29 浙江工业大学 一种栖热腔菌磷酸酶突变体及其应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
ZA876216B (en) * 1986-08-22 1989-04-26 Cetus Corp Purified thermostable enzyme
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
IL88923A (en) * 1988-01-12 1995-07-31 Hoffmann La Roche Gene encoding a thermostable dna polymerase from thermus aquaticus said dna polymerase and its purification
US5108892A (en) * 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
US5210036A (en) * 1990-04-26 1993-05-11 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
ES2134198T3 (es) * 1990-09-28 1999-10-01 Hoffmann La Roche Mutaciones en la 5' a 3' exonucleasa de las adn polimerasas.

Also Published As

Publication number Publication date
AU658378B2 (en) 1995-04-13
JPH06504196A (ja) 1994-05-19
WO1992006202A1 (en) 1992-04-16
AU8907791A (en) 1992-04-28
DE69132477T2 (de) 2001-06-13
JP2582980B2 (ja) 1997-02-19
EP0550696B1 (en) 2000-11-22
CA2092317A1 (en) 1992-03-29
DE69132477D1 (en) 2000-12-28
US5968799A (en) 1999-10-19
EP0550696A1 (en) 1993-07-14
ATE197718T1 (de) 2000-12-15
ES2152923T3 (es) 2001-02-16

Similar Documents

Publication Publication Date Title
DK0550696T3 (da) Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus
ATE233816T1 (de) Gereinigtes thermostabiles nukleinsäure- polymeraseenzym aus thermotoga maritima
ATE135741T1 (de) Gereinigtes thermostabiles enzym
ATE203056T1 (de) Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität
GB2301590B (en) Thermostable DNA Polymerases
DK438487A (da) Oprenset, termostabilt enzym fremgangsmaade til amplifikation, paavisning og/eller kloning af nukleinsyresekvenser under anvendelse af dette enzym
DK2418287T3 (da) Nucleinsyre-amplifikationsprimere til PCR-baseret klonalitetsundersøgelser af TCR-betagenet
DE69628959D1 (de) Stabilisierte Zusammensetzungen von Reverser Transkriptase und RNA Polymerase für Nukleinsäureamplifikation
ATE557083T1 (de) Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität
ATE151112T1 (de) Bei hoher temperatur aktive reverse transkriptasen
DK0667910T3 (da) En Bacillus-promoter, afledt fra en variant af en alpha-amylase-promoter fra Bacillus licheniformis
WO1995004162A3 (en) Dna sequencing enzymes
ATE81778T1 (de) Verwendung einer adenosin, hypoxanthin und ribose enthaltender loesung zur schonung des herzens waehrend chirurgischer eingriffe.
DK731988D0 (da) Amperometrisk fremgangsmaade til kvantitativ bestemmelse af 1,4-dihydronikotinamid-adenin-dinukleotid (nadh) i oploesning
EP0754765A3 (en) DNA sequencing and DNA terminators
NZ334653A (en) Thermostable nucleic acid polymerase with 3'-5' proofreading exonuclease ability from Thermococcus gorgonarius
ATE311477T1 (de) Hohe dichte markierung von dns mit modifizierte oder chromophore tragende nukleotide und benutzte dns polymerasen
CA2089495A1 (en) Purified thermostable nucleic acid polymerase enzyme from thermotoga maritima
MAURER et al. Fracture and the role of microstructure; Proceedings of the Fourth European Conference on Fracture, Leoben, Austria, September 22-24, 1982. Volumes 1 & 2
IL112750A (en) Nucleic acid detection kit