IL112750A - Nucleic acid detection kit
- Google Patents
Nucleic acid detection kit
Info
Publication number
IL112750A
IL112750AIL112750AIL11275089AIL112750AIL 112750 AIL112750 AIL 112750AIL 112750 AIL112750 AIL 112750AIL 11275089 AIL11275089 AIL 11275089AIL 112750 AIL112750 AIL 112750A
Authority
IL
Israel
Prior art keywords
nucleic acid
sequence
target
detection kit
acid detection
Prior art date
Application number
IL112750A
Original Assignee
Akzo Nobel Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Akzo Nobel NvfiledCriticalAkzo Nobel Nv
Priority claimed from IL92732Aexternal-prioritypatent/IL92732A/en
Publication of IL112750ApublicationCriticalpatent/IL112750A/en
Measuring Or Testing Involving Enzymes Or Micro-Organisms
(AREA)
Abstract
A kit useful for the detection of at least one specific nucleic acid target sequence in a RNA sample that may contain said nucleic acid target sequence, comprising a reaction mixture comprising a reverse transcriptase, an enzyme having RNase H activity, a DNA-dependent RNA polymerase and at least one DNA primer containing a promoter sequence operatively associated with a sequence complementary to a segment of a target nucleic acid sequence or its complement and means whereby upon contact of said mixture with sample for detection of target nucleic acid sequence, target nucleic acid sequence is detected after amplification.