DK438487A - Oprenset, termostabilt enzym fremgangsmaade til amplifikation, paavisning og/eller kloning af nukleinsyresekvenser under anvendelse af dette enzym - Google Patents

Oprenset, termostabilt enzym fremgangsmaade til amplifikation, paavisning og/eller kloning af nukleinsyresekvenser under anvendelse af dette enzym

Info

Publication number
DK438487A
DK438487A DK438487A DK438487A DK438487A DK 438487 A DK438487 A DK 438487A DK 438487 A DK438487 A DK 438487A DK 438487 A DK438487 A DK 438487A DK 438487 A DK438487 A DK 438487A
Authority
DK
Denmark
Prior art keywords
enzym
nucleic acid
thermostable
cloning
amplification
Prior art date
Application number
DK438487A
Other languages
English (en)
Other versions
DK438487D0 (da
DK175806B1 (da
Inventor
Henry Anthony Erlich
Glenn Horn
Randall Keichi Saiki
Kary Banks Mullis
David Harrow Gelfand
Frances Cook Lawyer
Susanne Stoffel
Original Assignee
Cetus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27490366&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK438487(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US07/063,509 external-priority patent/US4889818A/en
Priority claimed from US07/063,647 external-priority patent/US4965188A/en
Application filed by Cetus Corp filed Critical Cetus Corp
Publication of DK438487D0 publication Critical patent/DK438487D0/da
Publication of DK438487A publication Critical patent/DK438487A/da
Application granted granted Critical
Publication of DK175806B1 publication Critical patent/DK175806B1/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK198704384A 1986-08-22 1987-08-21 Oprenset termostabilt enzym og fremgangsmåde til amplifikation, påvisning og/eller kloning af nukleinsyresekvensen under anvendelse af dette enzym DK175806B1 (da)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US89951386A 1986-08-22 1986-08-22
US89924186A 1986-08-22 1986-08-22
US89924186 1986-08-22
US89951386 1986-08-22
US6350987 1987-06-17
US6364787 1987-06-17
US07/063,509 US4889818A (en) 1986-08-22 1987-06-17 Purified thermostable enzyme
US07/063,647 US4965188A (en) 1986-08-22 1987-06-17 Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme

Publications (3)

Publication Number Publication Date
DK438487D0 DK438487D0 (da) 1987-08-21
DK438487A true DK438487A (da) 1988-02-23
DK175806B1 DK175806B1 (da) 2005-03-07

Family

ID=27490366

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198704384A DK175806B1 (da) 1986-08-22 1987-08-21 Oprenset termostabilt enzym og fremgangsmåde til amplifikation, påvisning og/eller kloning af nukleinsyresekvensen under anvendelse af dette enzym

Country Status (17)

Country Link
EP (2) EP0258017B2 (da)
JP (5) JPH0824570B2 (da)
KR (1) KR960016559B1 (da)
CN (1) CN87105787A (da)
AT (2) ATE391771T1 (da)
AU (1) AU7729887A (da)
BR (1) BR8704332A (da)
CA (1) CA1338457C (da)
DE (4) DE258017T1 (da)
DK (1) DK175806B1 (da)
ES (2) ES2105998T1 (da)
GR (1) GR3024616T3 (da)
IE (1) IE970680A1 (da)
IL (1) IL83605A0 (da)
NO (1) NO305488B1 (da)
NZ (1) NZ221517A (da)
SG (1) SG46644A1 (da)

Families Citing this family (225)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5407800A (en) * 1986-08-22 1995-04-18 Hoffmann-La Roche Inc. Reverse transcription with Thermus thermophilus polymerase
US5618711A (en) * 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
CA1338457C (en) * 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US5322770A (en) * 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5795762A (en) * 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5079352A (en) * 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
AU632857C (en) * 1986-08-22 2003-10-09 F. Hoffmann-La Roche Ag Purified thermostable enzyme
US6127155A (en) * 1986-08-22 2000-10-03 Roche Molecular Systems, Inc. Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents
US5352600A (en) * 1986-08-22 1994-10-04 Hoffmann-La Roche Inc. Purified thermostable enzyme
US5310652A (en) * 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US4942130A (en) * 1987-01-14 1990-07-17 President & Fellows Of Harvard College T7 DNA polymerase
US4946786A (en) * 1987-01-14 1990-08-07 President And Fellows Of Harvard College T7 DNA polymerase
US4994372A (en) * 1987-01-14 1991-02-19 President And Fellows Of Harvard College DNA sequencing
US5145776A (en) * 1987-01-14 1992-09-08 President & Fellows Of Harvard College Method of using T7 DNA polymerase to mutagenize and fill-in DNA
US5266466A (en) * 1987-01-14 1993-11-30 President And Fellows Of Harvard College Method of using T7 DNA polymerase to label the 3' end of a DNA molecule
US4921794A (en) * 1987-01-14 1990-05-01 President And Fellows Of Harvard College T7 DNA polymerase
FR2629458B2 (fr) * 1987-07-31 1991-08-09 Ire Celltarg Sa Nouvelles sondes d'acides nucleiques specifiques de differents types de virus de papillome humain
IL88923A (en) * 1988-01-12 1995-07-31 Hoffmann La Roche Gene encoding a thermostable dna polymerase from thermus aquaticus said dna polymerase and its purification
US4999290A (en) * 1988-03-31 1991-03-12 The Board Of Regents, The University Of Texas System Detection of genomic abnormalities with unique aberrant gene transcripts
US5109124A (en) * 1988-06-01 1992-04-28 Biogen, Inc. Nucleic acid probe linked to a label having a terminal cysteine
WO1989012697A1 (en) * 1988-06-22 1989-12-28 The Board Of Regents Of The University Of Washingt Method for detecting abnormal genes
US4962020A (en) * 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
US5498523A (en) * 1988-07-12 1996-03-12 President And Fellows Of Harvard College DNA sequencing with pyrophosphatase
US5763573A (en) * 1988-08-10 1998-06-09 Chiron Corporation GTPase activating protein fragments
US5760203A (en) * 1988-08-10 1998-06-02 Chiron Corporation Gap gene sequences
US5242818A (en) * 1988-08-26 1993-09-07 Toyo Boseki Kabushiki Kaisha Method of producing a thermostable DNA polymerase from Thermus thermophilus
JP2531246B2 (ja) * 1988-08-26 1996-09-04 東洋紡績株式会社 耐熱性ｄｎａポリメラ―ゼおよびその製法
US5066584A (en) * 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5075216A (en) * 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
US5091310A (en) * 1988-09-23 1992-02-25 Cetus Corporation Structure-independent dna amplification by the polymerase chain reaction
US5142033A (en) * 1988-09-23 1992-08-25 Hoffmann-La Roche Inc. Structure-independent DNA amplification by the polymerase chain reaction
US5389512A (en) * 1988-10-07 1995-02-14 Hoffman-La Roche Inc. Method for determining the relative amount of a viral nucleic acid segment in a sample by the polymerase chain reaction
US5077192A (en) * 1988-10-25 1991-12-31 The General Hospital Corporation Method of detecting antigenic, nucleic acid-containing macromolecular entities
GB8827167D0 (en) * 1988-11-21 1988-12-29 Apothekernes Lab In vitro mutagenesis
AU627809B2 (en) * 1988-12-16 1992-09-03 Abbott Laboratories Isolating thermostable enzymes from engineered mesophiles
JPH0630640B2 (ja) * 1989-02-06 1994-04-27 イーストマン コダック カンパニー 熱安定性ポリメラーゼｄｎａシーケエンシング反応濃厚物
CA2011818A1 (en) * 1989-03-17 1990-09-17 Fred T. Oakes Methods for purification, amplification and detection of a nucleic acid
EP0467953A4 (en) * 1989-04-12 1992-06-17 The President And Fellows Of Harvard College Improved primer extension reactions
CA2013317A1 (en) * 1989-04-17 1990-10-17 Fred T. Oakes Diagnostic kit, primer composition and their use for replication or detection of nucleic acids
US5091302A (en) * 1989-04-27 1992-02-25 The Blood Center Of Southeastern Wisconsin, Inc. Polymorphism of human platelet membrane glycoprotein iiia and diagnostic and therapeutic applications thereof
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
ES2091243T3 (es) * 1989-05-22 1996-11-01 Hoffmann La Roche Metodos de señalizacion y rastreo de materiales mediante acidos nucleicos.
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5683896A (en) 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US6589734B1 (en) 1989-07-11 2003-07-08 Gen-Probe Incorporated Detection of HIV
KR100242252B1 (ko) * 1989-07-11 2000-03-02 다니엘 엘. 캐시앙 핵산서열의 증폭방법
US5108892A (en) * 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
GB8917963D0 (en) * 1989-08-05 1989-09-20 Scras Apparatus for repeated automatic execution of a thermal cycle for treatment of biological samples
US5213961A (en) * 1989-08-31 1993-05-25 Brigham And Women's Hospital Accurate quantitation of RNA and DNA by competetitive polymerase chain reaction
GB8926269D0 (en) * 1989-11-21 1990-01-10 Dynal As Plasmid
SG46627A1 (en) * 1989-12-22 1998-02-20 Hoffmann La Roche Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase
DE69030386T2 (de) * 1989-12-22 1997-10-09 F. Hoffmann-La Roche Ag, Basel Bei hoher temperatur aktive reverse transkriptasen
AU653712B2 (en) * 1990-02-16 1994-10-13 F. Hoffmann-La Roche Ag Improvements in the specificity and convenience of the polymerase chain reaction
US5049490A (en) * 1990-02-20 1991-09-17 Eastman Kodak Co. Quantitative determination of a DNA polymerase and a test kit useful in same
US5352778A (en) * 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
US5756334A (en) * 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
US5500363A (en) * 1990-04-26 1996-03-19 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
US5322785A (en) * 1990-04-26 1994-06-21 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
DE69126064T2 (de) * 1990-06-22 1997-08-28 Hoffmann La Roche Nachweis von schwachen Metabolisierungsreagentien von Drogen
US5844108A (en) * 1990-06-22 1998-12-01 Roche Molecular Systems, Inc. Primers targeted to NAT2 gene for detection of poor metabolizers of drugs
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
FR2669347A1 (fr) * 1990-11-15 1992-05-22 Inst Nat Sante Rech Med Procede permettant d'effectuer au moins deux reactions successives, en evitant les risques de contamination, notamment applicable aux techniques d'amplification de l'adn ou de l'arn.
KR100236506B1 (ko) * 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
US5464936A (en) * 1990-12-21 1995-11-07 Cetus Oncology Corporation Compositions for identification of papillomavirus replication inhibitors
EP0584238A1 (en) * 1991-05-17 1994-03-02 Chiron Corporation INHIBITOR OF NF-$g(k)B TRANSCRIPTIONAL ACTIVATOR AND USES THEREOF
EP0547359B1 (en) * 1991-12-18 2002-03-27 New England Biolabs, Inc. Purified thermostable dna polymerase obtainable from pyrococcus species
WO1993015222A1 (fr) * 1992-01-29 1993-08-05 Institut National De La Sante Et De La Recherche Medicale (Inserm) Procede pour eviter les risques de contamination, notamment applicable aux techniques d'amplification de l'adn ou de l'arn
CA2102784A1 (en) * 1992-03-11 1993-09-12 Peng Liang Identifying, isolating and cloning messenger rnas
DE4212555A1 (de) * 1992-04-15 1993-10-21 Boehringer Mannheim Gmbh Verfahren zum Nachweis von Nukleinsäuren
US6207368B1 (en) 1992-08-04 2001-03-27 Beckman Coulter, Inc. Methods and reagents for controlling chain extension and ligation chain reactions
US6180338B1 (en) 1992-08-04 2001-01-30 Beckman Coulter, Inc. Method, reagent and kit for the detection and amplification of nucleic acid sequences
US5565339A (en) * 1992-10-08 1996-10-15 Hoffmann-La Roche Inc. Compositions and methods for inhibiting dimerization of primers during storage of polymerase chain reaction reagents
US5491086A (en) * 1993-05-14 1996-02-13 Hoffmann-La Roche Inc. Purified thermostable nucleic acid polymerase and DNA coding sequences from pyrodictium species
US5597694A (en) * 1993-10-07 1997-01-28 Massachusetts Institute Of Technology Interspersed repetitive element-bubble amplification of nucleic acids
DE4336266A1 (de) * 1993-10-23 1995-04-27 Boehringer Mannheim Gmbh Stabilisierte flüssige Mischungen für die Markierung von Nukleinsäuren
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
ES2072238T3 (es) * 1994-10-17 1997-01-16 Harvard College Adn-polimerasas que tienen un sitio de union con nucleotidos modificados para la secuenciacion de adn.
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US5948614A (en) * 1995-09-08 1999-09-07 Life Technologies, Inc. Cloned DNA polymerases from thermotoga maritima and mutants thereof
CN1047328C (zh) * 1996-01-16 1999-12-15 中国石油化工总公司 气相加氢制1,4－丁二醇的催化剂
US6277592B1 (en) 1996-07-31 2001-08-21 Purina Mills, Inc. Porcine leptin protein, nucleic acid sequences coding therefor and uses thereof
US6297027B1 (en) 1996-07-31 2001-10-02 Purina Mills, Inc. Bovine leptin protein, nucleic acid sequences coding therefor and uses thereof
WO1998006736A1 (en) 1996-08-14 1998-02-19 Life Technologies, Inc. Stable compositions for nucleic acid amplification and sequencing
EP0834571A1 (en) 1996-10-03 1998-04-08 Roche Diagnostics GmbH Thermostable nucleic acid polymerase from Thermococcus gorgonarius
US6171788B1 (en) 1997-01-28 2001-01-09 The Regents Of The University Of California Methods for the diagnosis, prognosis and treatment of glaucoma and related disorders
US7138511B1 (en) 1997-01-28 2006-11-21 The Regents Of The University Of California Nucleic acids, kits and methods for the diagnosis, prognosis and treatment of glaucoma and related disorders
US6475724B1 (en) 1997-01-28 2002-11-05 The Regents Of The University Of California Nucleic acids, kits, and methods for the diagnosis, prognosis and treatment of glaucoma and related disorders
US6306588B1 (en) 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
US6103468A (en) * 1997-10-07 2000-08-15 Labatt Brewing Company Limited Rapid two-stage polymerase chain reaction method for detection of lactic acid bacteria in beer
CN1278863A (zh) * 1997-11-12 2001-01-03 博士伦公司 用季化羟乙基纤维素和聚合双胍对接触镜片进行消毒
EP0921196A1 (en) 1997-12-02 1999-06-09 Roche Diagnostics GmbH Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction
US6242235B1 (en) 1998-06-24 2001-06-05 Promega Corp. Polymerase stabilization by polyethoxylated amine surfactants
US6207379B1 (en) 1998-09-11 2001-03-27 One Lambda Method for amplification of DNA
US6440715B1 (en) 1999-03-12 2002-08-27 New England Biolabs, Inc. Method for cloning and expression of Rhodothermus Obamensis DNA polymerase I large fragment in E. coli
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
CA2377707A1 (en) 1999-06-22 2000-12-28 Invitrogen Corporation Improved primers and methods for the detection and discrimination of nucleic acids
US6830902B1 (en) 1999-07-02 2004-12-14 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
CA2384838C (en) 1999-09-13 2006-07-18 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
DK1226236T3 (da) * 1999-10-20 2006-06-19 Novozymes As Polypeptider, der har glucanotransferase-aktivitet, og nucleinsyrer, som koder disse
CA2839066A1 (en) 1999-12-16 2001-06-21 Monsanto Technology Llc Hybrid promoter for use in plants
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
CA2412721A1 (en) 2000-06-26 2002-01-03 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AU2002230734A1 (en) 2000-12-11 2002-06-24 Alexion Pharmaceuticals, Inc. Nested oligonucleotides containing hairpin for nucleic acid amplification
JP2005503755A (ja) 2000-12-13 2005-02-10 ニューゲン テクノロジーズ， インコーポレイテッド 核酸配列の複数コピーを作製するための方法および組成物、ならびにそれらを検出する方法
US6794141B2 (en) 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification
US6635451B2 (en) 2001-01-25 2003-10-21 Abbott Laboratories Desaturase genes and uses thereof
AU2002252279B2 (en) 2001-03-09 2005-05-12 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences
US6617136B2 (en) 2001-04-24 2003-09-09 3M Innovative Properties Company Biological sample processing methods and compositions that include surfactants
DE60235168D1 (de) 2001-05-09 2010-03-11 Monsanto Technology Llc Metabolitentransporter
EP1950305A1 (en) 2001-05-09 2008-07-30 Monsanto Technology, LLC Tyr a genes and uses thereof
US7668697B2 (en) 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
EP1275735A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
DK1436404T3 (da) 2001-09-19 2010-03-08 Alexion Pharma Inc Manipulerede templates og deres anvendelse i single-primer amplifikation
US7414111B2 (en) 2001-09-19 2008-08-19 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
CA2460759C (en) 2001-09-24 2011-05-31 One Lambda Diagnostic probe detection system
US20030165859A1 (en) 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US7211656B2 (en) 2002-01-30 2007-05-01 Abbott Laboratories Desaturase genes, enzymes encoded thereby, and uses thereof
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
WO2003096797A2 (en) 2002-05-15 2003-11-27 Monsanto Technology Llc Method of increasing plant organ and seed size in a plant
WO2003100019A2 (en) 2002-05-24 2003-12-04 Invitrogen Corporation Nested pcr employing degradable primers
EP2345738A1 (en) 2002-08-05 2011-07-20 Quanta Biosciences, Inc. Improved compositions for in vitro amplification of nucleic acids
EP2380900A1 (en) 2002-09-23 2011-10-26 E. I. du Pont de Nemours and Company Isolation and use of ryanodine receptors
US7094879B2 (en) 2002-10-02 2006-08-22 Abbott Laboratories Genetically engineered P30 antigen, improved antigen cocktail, and uses thereof
DE10303974A1 (de) 2003-01-31 2004-08-05 Abbott Gmbh & Co. Kg Amyloid-β(1-42)-Oligomere, Verfahren zu deren Herstellung und deren Verwendung
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ， インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
AU2004203649B2 (en) 2003-08-12 2006-01-12 F. Hoffmann-La Roche Ag Thermostable Taq polymerase fragment
US7807874B2 (en) 2003-12-10 2010-10-05 Monsanto Technology Llc Stress tolerant plants and methods thereof
ZA200609413B (en) * 2004-05-13 2008-09-25 Goel Anita Nano-PCR: Methods and devices for nucleic acid amplification and detection
PT1766023E (pt) 2004-06-04 2010-12-09 Fluxome Sciences As Células metabolicamente manipuladas para a produção de ácidos gordos poliinsaturados
US7456270B2 (en) 2004-09-01 2008-11-25 Abbott Laboratories Δ6-desaturase genes and uses thereof
EP1802775B1 (en) 2004-09-14 2010-05-26 The Regents of the University of Colorado Method for treatment with bucindolol based on genetic targeting
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US7790363B2 (en) 2005-02-07 2010-09-07 Abbott Laboratories Inc. Diagnostic test for vitamin B12
US8624027B2 (en) 2005-05-12 2014-01-07 Abbvie Inc. Combination therapy for treating cancer and diagnostic assays for use therein
AU2006279420B2 (en) 2005-08-16 2010-12-16 Merlogen, Llc Methods for identification of merle gene
CA2621267A1 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
DK1976877T4 (da) 2005-11-30 2017-01-16 Abbvie Inc Monoklonale antistoffer mod amyloid-beta-protein og anvendelser heraf
KR20080090408A (ko) 2005-11-30 2008-10-08 아보트 러보러터리즈 항-Ａβ 글로불로머 항체, 이의 항원-결합 잔기, 상응하는하이브리도마, 핵산, 벡터, 숙주 세포, 당해 항체의 제조방법, 당해 항체를 포함하는 조성물, 당해 항체의 용도 및당해 항체의 사용 방법
US7285388B1 (en) 2006-04-07 2007-10-23 Merlogen, Llc Methods for identification of alport syndrome
US8933209B2 (en) 2006-04-26 2015-01-13 Abbvie Inc. Dep2 and its uses in major depressive disorder and other related disorders
CA2909340C (en) 2006-05-25 2019-07-02 Monsanto Technology Llc A method to identify disease resistant quantitative trait loci in soybean and compositions thereof
US20080038163A1 (en) 2006-06-23 2008-02-14 Applera Corporation Systems and Methods for Cooling in Biological Analysis Instruments
JP4918409B2 (ja) 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
JP4863138B2 (ja) * 2006-10-26 2012-01-25 アイシン・エィ・ダブリュ工業株式会社 ロックアップダンパ及びダンパスプリング間に介在する中間支持部
US8455626B2 (en) 2006-11-30 2013-06-04 Abbott Laboratories Aβ conformer selective anti-aβ globulomer monoclonal antibodies
US20100311767A1 (en) 2007-02-27 2010-12-09 Abbott Gmbh & Co. Kg Method for the treatment of amyloidoses
US8871470B2 (en) 2007-03-21 2014-10-28 Applied Biosystems, Llc Adaptive thermal block temperature control method and system
BRPI0810083B8 (pt) 2007-03-28 2020-10-06 Monsanto Technology Llc métodos para estabelecer onde uma planta de soja ou semente de soja deve ser cultivada, de melhoramento da planta de soja, para selecionar uma semente de soja, de distribuição de uma planta de soja, para isolar sementes de soja de maturidade precoce indeterminada, e para determinar se uma planta de soja tem um grupo de maturidade
CL2008002553A1 (es) 2007-08-29 2009-09-04 Monsanto Technology Llc Metodo de mejoramiento de plantas mediante la introduccion de loci de caracteristicas cuantitativas asociadas con resistencia a la mancha foliar gris.
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US8268981B2 (en) 2008-03-06 2012-09-18 Abbott Laboratories Plasmodium malariae and plasmodium ovale genes and uses thereof
US8030471B2 (en) 2008-03-06 2011-10-04 Abbott Laboratories Plasmodium malariae and Plasmodium ovale genes and uses thereof
GB2470672B (en) 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
CA2991818C (en) 2008-03-28 2022-10-11 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
EP2108451A1 (de) 2008-04-11 2009-10-14 Eppendorf AG Vorrichtung zum Durchführen von Reaktionen in Proben
US8669414B2 (en) 2008-04-24 2014-03-11 Monsanto Technology Llc Method to identify Asian soybean rust resistance quantitative trait loci in soybean and compositions thereof
AU2009302522B2 (en) 2008-10-06 2015-03-26 Abbott Laboratires Delta-8 desaturase genes, enzymes encoded thereby and uses thereof
US8288124B2 (en) 2008-11-20 2012-10-16 Abbott Laboratories Cloning, expression and purification of recombinant porcine intrinsic factor for use in diagnostic assay
EP2371951A4 (en) 2008-11-27 2012-07-18 Riken NOVEL MUTS PROTEIN AND METHOD OF USING SAME FOR DETERMINING MUTATIONS
US9084781B2 (en) 2008-12-10 2015-07-21 Novartis Ag MEK mutations conferring resistance to MEK inhibitors
CA2749945A1 (en) 2009-01-31 2010-08-05 Abbott Laboratories Markers to predict and monitor response to aurora kinase b inhibitor therapy
JP2012517241A (ja) 2009-02-11 2012-08-02 アボット・ラボラトリーズ Ｂｃｌ−２ファミリー阻害剤耐性腫瘍及び癌を有する被験者を同定、分類及びモニターするための方法及び組成物
US8063193B2 (en) 2009-03-27 2011-11-22 Abbott Laboratories Nucleotide and amino acid sequences encoding an exported protein 1 derived from Plasmodium vivax and uses thereof
EP2430452B1 (en) 2009-05-14 2014-06-25 Arizona Board of Regents on Behalf of University of Arizona Carcinoma diagnosis and treatments, based on odc1 genotype
IT1394539B1 (it) 2009-05-19 2012-07-05 Sentinel Ch S P A Uso di uno stabilizzante delle polimerasi per la liofilizzazione e la preparazione di kit pronti per l'uso
EP2449381A1 (en) 2009-06-30 2012-05-09 Abbott Laboratories Markers of xmrv infection and uses thereof
US8188335B2 (en) 2009-07-17 2012-05-29 Abbott Laboratories Δ9-elongase for production of polyunsaturated fatty acid-enriched oils
US9512481B2 (en) 2009-09-11 2016-12-06 The Regents Of The University Of Colorado, A Body Corporate Polymorphisms in the PDE3A gene
CA2822747A1 (en) 2009-12-23 2011-06-30 Arca Biopharma, Inc. Use of s-(6-nitro-oxi-hexahydro-furo[3,2-b]thioacetate in the treatment of cardiovascular disorders associated with oxide synthase dysfunction
EP3028699B1 (en) 2010-02-25 2018-03-21 Dana-Farber Cancer Institute, Inc. Braf mutations conferring resistance to braf inhibitors
ES2714875T3 (es) 2010-03-09 2019-05-30 Dana Farber Cancer Inst Inc Métodos de diagnóstico y tratamiento del cáncer en pacientes que presentan o desarrollan resistencia a una primera terapia del cáncer
JP2013523182A (ja) 2010-04-15 2013-06-17 アボット・ラボラトリーズ アミロイドベータ結合タンパク質
EP2568978B1 (en) 2010-05-14 2019-04-24 Arizona Board of Regents on Behalf of University of Arizona Cancer prevention and treatment based on dietary polyamine content
EA028080B1 (ru) 2010-06-09 2017-10-31 Дана-Фарбер Кэнсер Инститьют, Инк. Мутация в mek1, придающая устойчивость к ингибиторам raf и mek
CN105348387B (zh) 2010-08-14 2020-08-25 Abbvie 公司 β淀粉样蛋白结合蛋白
WO2012024518A1 (en) 2010-08-18 2012-02-23 Abbott Laboratories Molecular detection of xmrv infection
WO2012024513A2 (en) 2010-08-18 2012-02-23 Abbott Laboratories Molecular detection of xmrv infection
WO2012146260A1 (de) 2011-04-23 2012-11-01 Biolytix Ag Herstellung und verwendung von proteinen in der molekularbiologie
WO2012149193A2 (en) 2011-04-29 2012-11-01 Monsanto Technology Llc Diagnostic molecular markers for seed lot purity traits in soybeans
WO2012154735A2 (en) * 2011-05-10 2012-11-15 Danisco Us Inc. Thermostable carbonic anhydrases and methods of use thereof
EP3461807B1 (en) 2011-06-08 2023-07-12 Life Technologies Corporation Design and development of novel detergents for use in pcr systems
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
HUE059863T2 (hu) 2011-08-31 2023-01-28 Seminis Vegetable Seeds Inc Eljárás és készítmények görögdinnye keménységhez
US10314253B2 (en) 2012-12-04 2019-06-11 Seminis Vegetable Seeds, Inc. Methods and compositions for watermelon sex expression
WO2014140309A1 (en) * 2013-03-15 2014-09-18 Sygnis Biotech S.L.U. Methods for amplification and sequencing using thermostable tthprimpol
US10294489B2 (en) 2013-03-15 2019-05-21 Board Of Trustees Of Southern Illinois University Soybean resistant to cyst nematodes
US10059999B2 (en) 2013-06-10 2018-08-28 Monsanto Technology Llc Molecular markers associated with soybean tolerance to low iron growth conditions
US9914964B2 (en) 2013-10-25 2018-03-13 Life Technologies Corporation Compounds for use in PCR systems and applications thereof
NZ791803A (en) 2013-11-27 2023-02-24 Seminis Vegetable Seeds Inc Disease resistance loci in onion
CA2939580C (en) 2014-02-21 2020-07-21 Syngenta Participations Ag Genetic loci associated with increased fertility in maize
NZ630710A (en) 2014-02-27 2016-03-31 Seminis Vegetable Seeds Inc Compositions and methods for peronospora resistance in spinach
US10316369B2 (en) 2014-06-27 2019-06-11 Seminis Vegetable Seeds, Inc. Methods and assays for male sterile watermelon
EP3005862A1 (en) 2014-10-10 2016-04-13 Seminis Vegetable Seeds, Inc. Melon plants with improved disease tolerance
US10448595B2 (en) 2015-09-03 2019-10-22 Seminis Vegetable Seeds, Inc. Downy mildew resistant lettuce plants
EP3368896A1 (en) 2015-10-30 2018-09-05 FMC Corporation Dihydroorotate dehydrogenase inhibitor compositions effective as herbicides
EP3199642A1 (en) 2016-02-01 2017-08-02 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Plant breeding using high throughput sequencing
SG11201808306PA (en) 2016-03-24 2018-10-30 Tragara Pharmaceuticals Inc Treatment of cancer with tg02
US10633386B2 (en) 2016-04-12 2020-04-28 The Regents Of The University Of Michigan BET protein degraders
JP6961684B2 (ja) 2016-09-13 2021-11-05 ザ リージェンツ オブ ザ ユニヴァシティ オブ ミシガン Ｂｅｔタンパク質分解物質としての縮合１，４−オキサゼピン
EP3858837A1 (en) 2016-09-13 2021-08-04 The Regents of The University of Michigan Fused 1,4-diazepines as bet protein degraders
AU2017232187B2 (en) 2016-09-30 2023-11-09 Seminis Vegetable Seeds, Inc. Xanthomonas resistant brassica oleracea plants
WO2018094008A1 (en) 2016-11-21 2018-05-24 Fmc Corporation Cellulose synthase inhibitors effective as herbicides
CN108624640B (zh) * 2017-03-15 2021-09-21 中国科学院微生物研究所 一种利用嗜热引发酶扩增dna的方法
EA202090485A1 (ru) 2017-08-14 2020-05-27 Эпизайм, Инк. Способы лечения рака путем ингибирования setd2
JP7250764B2 (ja) 2017-08-18 2023-04-03 コセラ バイオサイエンス， インコーポレイテッド Ｔｇ０２の多形形態
US11267822B2 (en) 2017-09-13 2022-03-08 The Regents Of The University Of Michigan BET bromodomain protein degraders with cleavable linkers
SG11202103282YA (en) 2018-10-08 2021-04-29 Univ Michigan Regents Small molecule mdm2 protein degraders
AU2020208190B2 (en) 2019-01-15 2022-07-28 Seminis Vegetable Seeds, Inc. Green bean plants with improved disease resistance
EP3983084A1 (en) 2019-06-12 2022-04-20 Vanderbilt University Amino acid transport inhibitors and the uses thereof
CA3141414A1 (en) 2019-06-12 2020-12-17 Vanderbilt University Dibenzylamines as amino acid transport inhibitors
US11542513B2 (en) 2019-09-26 2023-01-03 Seminis Vegetable Seeds, Inc. Lettuce plants having resistance to Nasonovia ribisnigri biotype Nr:1
CN110938603B (zh) * 2019-12-20 2022-11-22 阿吉安(福州)基因医学检验实验室有限公司 一种通用型病毒样本保存缓冲液及其制备方法
AU2021204717A1 (en) 2020-07-15 2022-02-03 Seminis Vegetable Seeds, Inc. Green Bean Plants with Improved Disease Resistance
CN114606575A (zh) * 2020-12-03 2022-06-10 广东菲鹏生物有限公司 组合物及文库构建方法
US20230193310A1 (en) 2021-12-10 2023-06-22 Seminis Vegetabe Seeds, Inc. Lettuce plants having resistance to downy mildew
US20230404003A1 (en) 2022-06-21 2023-12-21 Seminis Vegetable Seeds, Inc. Novel qtls conferring resistance to cucumber mosaic virus

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS51104087A (ja) * 1976-02-02 1976-09-14 Toyo Boseki Kosososeibutsunoseizohoho
US4305837A (en) * 1980-10-30 1981-12-15 The Procter & Gamble Company Stabilized aqueous enzyme composition
US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4735801A (en) * 1982-09-07 1988-04-05 Board Of Trustees Of Leland Stanford Jr. University Novel non-reverting salmonella live vaccines
US4358535A (en) 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
US4419446A (en) 1980-12-31 1983-12-06 The United States Of America As Represented By The Department Of Health And Human Services Recombinant DNA process utilizing a papilloma virus DNA as a vector
CA1219824A (en) 1981-04-17 1987-03-31 David C. Ward Modified nucleotides and methods of preparing and using same
US4601978A (en) 1982-11-24 1986-07-22 The Regents Of The University Of California Mammalian metallothionein promoter system
JPS60160884A (ja) * 1984-02-01 1985-08-22 Showa Denko Kk 酵素安定化法
US4582789A (en) 1984-03-21 1986-04-15 Cetus Corporation Process for labeling nucleic acids using psoralen derivatives
US4617261A (en) 1984-03-21 1986-10-14 Cetus Corporation Process for labeling nucleic acids and hybridization probes
JPS60224499A (ja) * 1984-04-23 1985-11-08 Toyobo Co Ltd 安定なウリカ−ゼ製剤
US4683194A (en) 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
EP0196864A3 (en) 1985-03-25 1988-03-23 Cetus Corporation Alkaline phosphatase-mediated processing and secretion of recombinant proteins, dna sequences for use therein and cells transformed using such sequences
AU606043B2 (en) * 1985-03-28 1991-01-31 F. Hoffmann-La Roche Ag Detection of viruses by amplification and hybridization
DK171161B1 (da) 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
CA1284931C (en) * 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
CA1338457C (en) * 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme

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