DE69613856T2 - Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität - Google Patents

Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität

Info

Publication number
DE69613856T2
DE69613856T2 DE69613856T DE69613856T DE69613856T2 DE 69613856 T2 DE69613856 T2 DE 69613856T2 DE 69613856 T DE69613856 T DE 69613856T DE 69613856 T DE69613856 T DE 69613856T DE 69613856 T2 DE69613856 T2 DE 69613856T2
Authority
DE
Germany
Prior art keywords
dna polymerase
muted
enzymes
derived
exonuclease activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69613856T
Other languages
English (en)
Other versions
DE69613856D1 (de
Inventor
A Mamone
Maria Davis
Dan Sha
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
Amersham Pharmacia Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Pharmacia Biotech Inc filed Critical Amersham Pharmacia Biotech Inc
Publication of DE69613856D1 publication Critical patent/DE69613856D1/de
Application granted granted Critical
Publication of DE69613856T2 publication Critical patent/DE69613856T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
DE69613856T 1995-12-15 1996-12-13 Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität Expired - Lifetime DE69613856T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US868895P 1995-12-15 1995-12-15
PCT/US1996/020225 WO1997021821A1 (en) 1995-12-15 1996-12-13 Thermostable dna polymerase from thermoanaerobacter thermohydrosulfuricus and mutant enzymes thereof with exonuclease activity removed

Publications (2)

Publication Number Publication Date
DE69613856D1 DE69613856D1 (de) 2001-08-16
DE69613856T2 true DE69613856T2 (de) 2002-04-04

Family

ID=21733102

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69613856T Expired - Lifetime DE69613856T2 (de) 1995-12-15 1996-12-13 Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität

Country Status (8)

Country Link
US (1) US5744312A (de)
EP (1) EP0866868B1 (de)
JP (1) JP4073038B2 (de)
AT (1) ATE203056T1 (de)
CA (1) CA2240334C (de)
DE (1) DE69613856T2 (de)
ES (1) ES2162132T3 (de)
WO (1) WO1997021821A1 (de)

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US6406891B1 (en) 1998-09-28 2002-06-18 Board Of Regents, The University Of Texas System Dual RT procedure for cDNA synthesis
US6238868B1 (en) * 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
KR100527265B1 (ko) 1999-09-13 2005-11-09 뉴젠 테크놀로지스 인코포레이티드 폴리뉴클레오티드 서열의 선형 등온 증폭을 위한 방법 및조성물
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
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US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
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US6858413B2 (en) * 2000-12-13 2005-02-22 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US6794141B2 (en) 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification
CA2439074A1 (en) * 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
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WO2002072892A1 (en) 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
KR20010079080A (ko) * 2001-06-12 2001-08-22 김유삼 신규한 dna 중합효소의 dna 서열 및 그로부터 유래된 단백질
EP1436385A4 (de) * 2001-09-14 2005-12-14 Invitrogen Corp Dna-polymerasen und mutanten davon
US20030157533A1 (en) * 2001-12-20 2003-08-21 Maria Davis Nucleic acid labeling by Thermoanaerobacter thermohydrosulfuricus DNA polymerase I variants
US20030180741A1 (en) 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
US20060246434A1 (en) * 2002-03-15 2006-11-02 Erlander Mark G Nucleic acid amplification
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
CA2521084A1 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US7169560B2 (en) * 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
DE602005027700D1 (de) 2004-05-25 2011-06-09 Helicos Biosciences Corp Verfahren zur nukleinsäureimmobilisierung
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
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BRPI0515777A (pt) * 2004-12-11 2008-08-05 Cytogenix Inc biossìntese isenta de células de ácido nucléico de alta qualidade e usos dos mesmos
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2007030759A2 (en) * 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
CA2624324A1 (en) * 2005-10-06 2007-04-19 Lucigen Corporation Thermostable viral polymerases and methods of use
US20090305248A1 (en) * 2005-12-15 2009-12-10 Lander Eric G Methods for increasing accuracy of nucleic acid sequencing
WO2007117331A2 (en) * 2005-12-22 2007-10-18 Ge Healthcare Bio-Sciences Corp. Novel dna polymerase from thermoanaerobacter tengcongenesis
US7397546B2 (en) * 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
WO2008008284A2 (en) 2006-07-14 2008-01-17 The Regents Of The University Of California Cancer biomarkers and methods of use threof
US8034568B2 (en) * 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
US20120003639A1 (en) 2010-04-27 2012-01-05 Prelude, Inc. Cancer biomarkers and methods of use thereof
US9777319B2 (en) 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template
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DE655506T1 (de) * 1994-10-17 1995-09-28 Harvard College DNS Polymerase mit Veränderten Nukleotiden Bindungstelle.

Also Published As

Publication number Publication date
ATE203056T1 (de) 2001-07-15
ES2162132T3 (es) 2001-12-16
JP4073038B2 (ja) 2008-04-09
DE69613856D1 (de) 2001-08-16
EP0866868B1 (de) 2001-07-11
CA2240334C (en) 2008-07-22
EP0866868A1 (de) 1998-09-30
JP2000501616A (ja) 2000-02-15
CA2240334A1 (en) 1997-06-19
US5744312A (en) 1998-04-28
WO1997021821A1 (en) 1997-06-19

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Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: AMERSHAM PHARMACIA BIOTECH INC., PISCATAWAY, N.J.,

8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: GE HEALTHCARE BIO-SCIENCES CORP. (N.D.GES.D.STAATE