BR9006818A - Amilases microbianas mutantes com maior estabilidade termica,acida e/ou alcalina - Google Patents

Amilases microbianas mutantes com maior estabilidade termica,acida e/ou alcalina

Info

Publication number
BR9006818A
BR9006818A BR909006818A BR9006818A BR9006818A BR 9006818 A BR9006818 A BR 9006818A BR 909006818 A BR909006818 A BR 909006818A BR 9006818 A BR9006818 A BR 9006818A BR 9006818 A BR9006818 A BR 9006818A
Authority
BR
Brazil
Prior art keywords
amylases
acid
alpha
greater thermal
alkaline stability
Prior art date
Application number
BR909006818A
Other languages
English (en)
Inventor
Wilhelmus Johannes Quax
Yves Laroche
Adrianus Wilhelmus Hermanus
Patrick Stanssens
Marc Lauwereys
Original Assignee
Gist Brocades Nv
Plant Genetic Systems Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades Nv, Plant Genetic Systems Nv filed Critical Gist Brocades Nv
Publication of BR9006818A publication Critical patent/BR9006818A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
BR909006818A 1989-06-29 1990-06-27 Amilases microbianas mutantes com maior estabilidade termica,acida e/ou alcalina BR9006818A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP89201735 1989-06-29
PCT/EP1990/001042 WO1991000353A2 (en) 1989-06-29 1990-06-27 MUTANT MICROBIAL α-AMYLASES WITH INCREASED THERMAL, ACID AND/OR ALKALINE STABILITY

Publications (1)

Publication Number Publication Date
BR9006818A true BR9006818A (pt) 1991-08-06

Family

ID=8202426

Family Applications (1)

Application Number Title Priority Date Filing Date
BR909006818A BR9006818A (pt) 1989-06-29 1990-06-27 Amilases microbianas mutantes com maior estabilidade termica,acida e/ou alcalina

Country Status (17)

Country Link
US (1) US5364782A (pt)
EP (1) EP0410498B1 (pt)
JP (2) JP3086249B2 (pt)
KR (1) KR0165550B1 (pt)
CN (1) CN1050220A (pt)
AT (1) ATE166922T1 (pt)
BG (1) BG61081B1 (pt)
BR (1) BR9006818A (pt)
CA (1) CA2030554C (pt)
DD (1) DD301620A9 (pt)
DE (1) DE69032360T2 (pt)
DK (1) DK0410498T3 (pt)
ES (1) ES2117625T3 (pt)
FI (1) FI103285B (pt)
IE (1) IE902369A1 (pt)
PT (1) PT94560B (pt)
WO (1) WO1991000353A2 (pt)

Families Citing this family (132)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992019744A1 (en) * 1991-05-06 1992-11-12 Valio, Finnish Co-Operative Dairies' Association ACID α-AMYLASE
JPH07503363A (ja) * 1991-11-14 1995-04-13 ノボザイムス アクティーゼルスカブ バシラス・リヘニフォルミス中で遺伝子を発現させる方法
WO1994013792A1 (en) * 1992-12-09 1994-06-23 Yoshiyuki Takasaki NOVEL ACID- AND HEAT-RESISTANT α-AMYLASE, PROCESS FOR PRODUCING THE SAME, AND METHOD OF LIQUEFYING STARCH BY USING THE SAME
DK154292D0 (da) * 1992-12-23 1992-12-23 Novo Nordisk As Nyt enzym
ES2126743T5 (es) * 1993-02-11 2010-02-05 Genencor International, Inc. Alfa-amilasa oxidativamente estable.
US5635468A (en) * 1993-05-19 1997-06-03 Kao Corporation Liquefying alkaline α-amylase, process for producing the same, and detergent composition containing the same
DE69434635D1 (en) * 1993-10-08 2006-04-27 Novo Nordisk As Amylasevarianten
US5830837A (en) * 1994-11-22 1998-11-03 Novo Nordisk A/S Amylase variants
WO1995010603A1 (en) * 1993-10-08 1995-04-20 Novo Nordisk A/S Amylase variants
TW268980B (pt) * 1994-02-02 1996-01-21 Novo Nordisk As
ES2250969T3 (es) * 1994-03-29 2006-04-16 Novozymes A/S Amilasa alcalina de bacilo.
TW255887B (en) * 1994-05-25 1995-09-01 Lilly Co Eli Synthesis of benzoquinolinones
EP0772684B1 (en) * 1994-06-17 2005-08-10 Genencor International, Inc. Amylolytic enzymes derived from the b. licheniformis alpha-amylase, having improved characteristics
WO1996017056A1 (en) * 1994-12-02 1996-06-06 Institut Pasteur Hypermutagenesis
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
US6093562A (en) * 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
AU4483496A (en) * 1995-02-03 1996-08-21 Novo Nordisk A/S A method of designing alpha-amylase mutants with predetermined properties
CN101363017A (zh) * 1995-02-03 2009-02-11 诺维信公司 淀粉酶变体
US6440716B1 (en) 1995-02-03 2002-08-27 Novozymes A/S α-amylase mutants
US7115409B1 (en) 1995-02-03 2006-10-03 Novozymes A/S α-amylase mutants
KR19980702782A (ko) * 1995-03-09 1998-08-05 혼 마가렛 에이. 녹말 액화 방법
US5736499A (en) * 1995-06-06 1998-04-07 Genencor International, Inc. Mutant A-amylase
JP3025627B2 (ja) 1995-06-14 2000-03-27 花王株式会社 液化型アルカリα−アミラーゼ遺伝子
AR003020A1 (es) * 1995-07-24 1998-05-27 Procter & Gamble Composicion detergente que comprende una amilasa de estabilidad oxidativa aumentada y un sistema surfactante especifico.
DK0850307T3 (da) * 1995-09-13 2006-03-06 Genencor Int Alkalifile og termofile mikroorganismer og enzymer, der opnås derfra
DK0904360T3 (en) 1996-04-30 2013-10-14 Novozymes As Alpha-amylasemutanter
US6197070B1 (en) 1996-05-15 2001-03-06 The Procter & Gamble Company Detergent compositions comprising alpha combination of α-amylases for malodor stripping
US5958739A (en) * 1996-06-06 1999-09-28 Genencor International Inc. Mutant α-amylase
CA2285579A1 (en) * 1997-04-28 1998-11-05 Wengui Yan Crop heterosis and herbicide
US6080568A (en) * 1997-08-19 2000-06-27 Genencor International, Inc. Mutant α-amylase comprising modification at residues corresponding to A210, H405 and/or T412 in Bacillus licheniformis
US6361989B1 (en) 1997-10-13 2002-03-26 Novozymes A/S α-amylase and α-amylase variants
ES2322825T3 (es) 1997-10-13 2009-06-29 Novozymes A/S Mutantes de alfa-amilasa.
BRPI9813328B1 (pt) 1997-10-30 2016-04-12 Novo Nordisk As variante de uma alfa-amilase, vetor de expressão recombinante, uso de uma variante de alfa-amilase, aditivo detergente, composição detergente, e, composição para lavagem de roupas manual ou automática
EP2287318B1 (en) 1998-06-10 2014-01-22 Novozymes A/S Mannanases
DK2290060T3 (en) 1999-03-30 2017-03-06 Novozymes As The alpha-amylase variants
US6410295B1 (en) 1999-03-30 2002-06-25 Novozymes A/S Alpha-amylase variants
EP1214441A1 (en) * 1999-09-01 2002-06-19 Novozymes A/S Maltogenic amylase-modified starch derivatives
WO2001066712A2 (en) 2000-03-08 2001-09-13 Novozymes A/S Variants with altered properties
BR0112778A (pt) 2000-07-28 2003-07-01 Henkel Kommanditgellschaft Auf Enzima amilolìtica de bacillus sp. a 7-7 (dsm 12368) bem como detergente e agente de limpeza com esta enzima amilolìtica
US20020155574A1 (en) * 2000-08-01 2002-10-24 Novozymes A/S Alpha-amylase mutants with altered properties
JP4855632B2 (ja) 2000-08-01 2012-01-18 ノボザイムス アクティーゼルスカブ 変更された性質を有するα−アミラーゼ突然変異体
AU2002233186A1 (en) 2000-11-28 2002-06-11 Henkel Kommanditgesellschaft Auf Aktien Cyclodextrin glucanotransferase (cgtase), obtained from bacillus agaradherens (dsm 9948) and detergents and cleaning agents containing said novel cyclodextrin glucanotransferase
JP3753945B2 (ja) 2001-02-14 2006-03-08 ヒゲタ醤油株式会社 大腸菌とブレビバチルス属細菌間のプラスミドシャトルベクター
EP2159279A3 (en) 2001-05-15 2010-05-12 Novozymes A/S Alpha-amylase variant with altered properties
DE10138753B4 (de) * 2001-08-07 2017-07-20 Henkel Ag & Co. Kgaa Wasch- und Reinigungsmittel mit Hybrid-Alpha-Amylasen
DE10163748A1 (de) 2001-12-21 2003-07-17 Henkel Kgaa Neue Glykosylhydrolasen
WO2004059074A1 (en) * 2002-12-20 2004-07-15 Novozymes North America, Inc. Treatment of fabrics, fibers, or yarns
PL1627050T3 (pl) * 2003-04-01 2013-10-31 Danisco Us Inc Wariant CBH1.1 Humicola grisea
JP4955546B2 (ja) 2004-07-05 2012-06-20 ノボザイムス アクティーゼルスカブ 変更された性質を有するα−アミラーゼ変異体
DE102004047777B4 (de) 2004-10-01 2018-05-09 Basf Se Alpha-Amylase-Varianten mit erhöhter Lösungsmittelstabilität, Verfahren zu deren Herstellung sowie deren Verwendung
CN100396777C (zh) * 2005-10-28 2008-06-25 南开大学 一种嗜热碱性α-淀粉酶及其编码基因
EP1876285A1 (de) * 2006-07-05 2008-01-09 DyStar Textilfarben GmbH & Co. Deutschland KG Kombinierte Entmineralisierung und Entschlichtung von Textilfasermaterialien
DK2215202T3 (da) 2007-11-05 2017-11-27 Danisco Us Inc VARIANTER AF BACILLUS sp. TS-23 ALPHA-AMYLASE MED ÆNDREDE EGENSKABER
BRPI0819184B1 (pt) 2007-11-05 2022-05-10 Danisco Us Inc Variantes de alfa-amilase com propriedades alteradas, polinucleotídeo, vetor, célula hospedeira, composição, kit, bem como métodos para tratamento de um caldo de amido, para produção de um substrato fermentável, e para tratamento de um material contendo amido
RU2526516C2 (ru) 2008-02-04 2014-08-20 ДАНИСКО ЮЭс ИНК. Варианты альфа-амилазы ts-23 с измененными свойствами
PL2297313T3 (pl) * 2008-06-06 2015-08-31 Danisco Us Inc Warianty alfa-amylazy z Bacillus subtilis i sposoby ich zastosowania
AU2009256280B2 (en) 2008-06-06 2013-03-07 Danisco Us Inc. Geobacillus stearothermophilus alpha-amylase (AmyS) variants with improved properties
WO2010036515A1 (en) 2008-09-25 2010-04-01 Danisco Us Inc. Alpha-amylase blends and methods for using said blends
WO2011076897A1 (en) 2009-12-22 2011-06-30 Novozymes A/S Use of amylase variants at low temperature
DK2521774T3 (en) 2010-01-04 2016-09-26 Novozymes As ALFA AMYLASE VARANTS AND POLYNUCLEOTIDES CODING THESE
CN113186178A (zh) 2010-02-10 2021-07-30 诺维信公司 在螯合剂存在下具有高稳定性的变体和包含变体的组合物
EP2357220A1 (en) 2010-02-10 2011-08-17 The Procter & Gamble Company Cleaning composition comprising amylase variants with high stability in the presence of a chelating agent
EP3421595B1 (en) 2011-06-30 2020-10-07 Novozymes A/S Alpha-amylase-variants
EP3110833B1 (en) 2013-05-29 2020-01-08 Danisco US Inc. Novel metalloproteases
US20160160202A1 (en) 2013-05-29 2016-06-09 Danisco Us Inc. Novel metalloproteases
JP6367930B2 (ja) 2013-05-29 2018-08-01 ダニスコ・ユーエス・インク 新規メタロプロテアーゼ
EP3882346A1 (en) 2013-05-29 2021-09-22 Danisco US Inc. Novel metalloproteases
US20160312204A1 (en) 2013-12-13 2016-10-27 Danisco Us Inc. Serine proteases of bacillus species
US10533165B2 (en) 2013-12-13 2020-01-14 Danisco Us Inc Serine proteases of the bacillus gibsonii-clade
CN106255707B (zh) 2013-12-16 2019-06-18 纳幕尔杜邦公司 聚α-1，3-葡聚糖醚类作为粘度调节剂的用途
JP6559139B2 (ja) 2013-12-18 2019-08-14 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ カチオン性ポリα−１，３−グルカンエーテル
WO2015123323A1 (en) 2014-02-14 2015-08-20 E. I. Du Pont De Nemours And Company Poly-alpha-1,3-1,6-glucans for viscosity modification
ES2734726T3 (es) 2014-03-11 2019-12-11 Du Pont Poli alfa-1,2-glucano oxidado como reforzante de detergente
EP3119884B1 (en) 2014-03-21 2019-07-10 Danisco US Inc. Serine proteases of bacillus species
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
EP3919599A1 (en) 2014-06-19 2021-12-08 Nutrition & Biosciences USA 4, Inc. Compositions containing one or more poly alpha-1,3-glucan ether compounds
EP3207129B1 (en) 2014-10-17 2019-11-20 Danisco US Inc. Serine proteases of bacillus species
EP3212782B1 (en) 2014-10-27 2019-04-17 Danisco US Inc. Serine proteases
EP3212783A1 (en) 2014-10-27 2017-09-06 Danisco US Inc. Serine proteases
DK3212662T3 (da) 2014-10-27 2020-07-20 Danisco Us Inc Serinproteaser
US20180010074A1 (en) 2014-10-27 2018-01-11 Danisco Us Inc. Serine proteases of bacillus species
US20170335306A1 (en) 2014-10-27 2017-11-23 Danisco Us Inc. Serine proteases
WO2016106013A1 (en) 2014-12-23 2016-06-30 E. I. Du Pont De Nemours And Company Enzymatically produced cellulose
EP3872174B1 (en) 2015-05-13 2023-03-01 Danisco US Inc. Aprl-clade protease variants and uses thereof
WO2016201040A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc. Water-triggered enzyme suspension
EP3307427B1 (en) 2015-06-09 2023-08-16 Danisco US Inc. Osmotic burst encapsulates
WO2016201069A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc Low-density enzyme-containing particles
US11499146B2 (en) 2015-06-17 2022-11-15 Danisco Us Inc. Bacillus gibsonii-clade serine proteases
JP7364331B2 (ja) 2015-11-05 2023-10-18 ダニスコ・ユーエス・インク パエニバチルス（Ｐａｅｎｉｂａｃｉｌｌｕｓ）属種マンナナーゼ
US20180320158A1 (en) 2015-11-05 2018-11-08 Danisco Us Inc. Paenibacillus and bacillus spp. mannanases
US10822574B2 (en) 2015-11-13 2020-11-03 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
WO2017083228A1 (en) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
EP3374488B1 (en) 2015-11-13 2020-10-14 DuPont Industrial Biosciences USA, LLC Glucan fiber compositions for use in laundry care and fabric care
US11920170B2 (en) 2015-12-09 2024-03-05 Danisco Us Inc. Alpha-amylase combinatorial variants
WO2017106676A1 (en) 2015-12-18 2017-06-22 Danisco Us Inc Polypeptides with endoglucanase activity and uses thereof
CN105802940B (zh) * 2016-04-18 2019-04-16 广西大学 一种地衣芽胞杆菌高温α-淀粉酶突变体及其应用
EP3452585A1 (en) 2016-05-03 2019-03-13 Danisco US Inc. Protease variants and uses thereof
EP3845642B1 (en) 2016-05-05 2023-08-09 Danisco US Inc. Protease variants and uses thereof
JP2019523645A (ja) 2016-05-31 2019-08-29 ダニスコ・ユーエス・インク プロテアーゼ変異体およびその使用
CA3027745A1 (en) 2016-06-17 2017-12-21 Danisco Us Inc. Protease variants and uses thereof
EP3535365A2 (en) 2016-11-07 2019-09-11 Danisco US Inc. Laundry detergent composition
EP3559227A1 (en) 2016-12-21 2019-10-30 Danisco US Inc. Protease variants and uses thereof
EP4212622A3 (en) 2016-12-21 2023-11-29 Danisco US Inc. Bacillus gibsonii-clade serine proteases
CN110621778A (zh) 2017-03-15 2019-12-27 丹尼斯科美国公司 胰蛋白酶样丝氨酸蛋白酶及其用途
WO2018183662A1 (en) 2017-03-31 2018-10-04 Danisco Us Inc Delayed release enzyme formulations for bleach-containing detergents
CN110809624A (zh) 2017-06-30 2020-02-18 丹尼斯科美国公司 低团聚的含酶颗粒
US20200354708A1 (en) 2017-11-29 2020-11-12 Danisco Us Inc. Subtilisin variants having improved stability
MX2020006518A (es) 2017-12-21 2020-10-28 Danisco Us Inc Gránulos de fusión en caliente, que contienen enzimas, que comprenden un desecante termotolerante.
WO2019156670A1 (en) 2018-02-08 2019-08-15 Danisco Us Inc. Thermally-resistant wax matrix particles for enzyme encapsulation
US20210214703A1 (en) 2018-06-19 2021-07-15 Danisco Us Inc Subtilisin variants
US20210363470A1 (en) 2018-06-19 2021-11-25 Danisco Us Inc Subtilisin variants
WO2020047215A1 (en) 2018-08-30 2020-03-05 Danisco Us Inc Enzyme-containing granules
US20220033737A1 (en) 2018-09-27 2022-02-03 Danisco Us Inc Compositions for medical instrument cleaning
EP3887515A1 (en) 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin variants having improved stability
WO2020242858A1 (en) 2019-05-24 2020-12-03 Danisco Us Inc Subtilisin variants and methods of use
WO2020247582A1 (en) 2019-06-06 2020-12-10 Danisco Us Inc Methods and compositions for cleaning
US20240034960A1 (en) 2020-08-27 2024-02-01 Danisco Us Inc Enzymes and enzyme compositions for cleaning
CN116997642A (zh) 2021-01-29 2023-11-03 丹尼斯科美国公司 清洁组合物及其相关的方法
WO2023278297A1 (en) 2021-06-30 2023-01-05 Danisco Us Inc Variant lipases and uses thereof
WO2023034486A2 (en) 2021-09-03 2023-03-09 Danisco Us Inc. Laundry compositions for cleaning
WO2023039270A2 (en) 2021-09-13 2023-03-16 Danisco Us Inc. Bioactive-containing granules
WO2023114939A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
WO2023114932A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
WO2023114936A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
WO2023168234A1 (en) 2022-03-01 2023-09-07 Danisco Us Inc. Enzymes and enzyme compositions for cleaning
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN114875057A (zh) * 2022-06-14 2022-08-09 中农华威生物制药(湖北)有限公司 一种可高效表达饲用低温酸性α-淀粉酶的枯草芽孢杆菌的构建方法
WO2023250301A1 (en) 2022-06-21 2023-12-28 Danisco Us Inc. Methods and compositions for cleaning comprising a polypeptide having thermolysin activity
WO2024050339A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Mannanase variants and methods of use
WO2024050346A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Detergent compositions and methods related thereto
WO2024050343A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Subtilisin variants and methods related thereto
CN117305279B (zh) * 2023-10-08 2024-03-26 态创生物科技(广州)有限公司 高活性和高耐热性的α-淀粉酶突变体及其制备方法和应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4394443A (en) * 1980-12-18 1983-07-19 Yale University Method for cloning genes
CA1170202A (en) * 1981-01-15 1984-07-03 Susan Mickel Process for cloning the gene coding for a thermostable alpha-amylase into escherichia coli and bacillus subtilis
WO1985000382A1 (en) * 1983-07-06 1985-01-31 Gist-Brocades N.V. Molecular cloning and expression in industrial microorganism species
US4740461A (en) * 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
US4717662A (en) * 1985-01-31 1988-01-05 Miles Laboratories, Inc. Thermal stabilization of alpha-amylase
EP0208491B1 (en) * 1985-07-03 1993-08-25 Genencor International, Inc. Hybrid polypeptides and process for their preparation
US5024943A (en) * 1985-11-07 1991-06-18 Gist-Brocades Regulatory region cloning and analysis plasmid for bacillus
DK311186D0 (da) * 1986-06-30 1986-06-30 Novo Industri As Enzymer
IL83192A (en) * 1986-07-18 1992-11-15 Gist Brocades Nv Method for the preparation of proteins with factor viii activity by microbial host cells;expression vectors,host cells,antibodies
EP0285123A3 (en) * 1987-04-03 1989-02-01 Stabra AG A method for complete mutagenesis of nucleic acids
WO1989001520A1 (en) * 1987-08-11 1989-02-23 Cetus Corporation Procaryotic xylose isomerase muteins and method to increase protein stability

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KR0165550B1 (ko) 1999-01-15
EP0410498B1 (en) 1998-06-03
IE902369A1 (en) 1991-06-19
DE69032360D1 (de) 1998-07-09
WO1991000353A2 (en) 1991-01-10
AU638263B2 (en) 1993-06-24
CA2030554A1 (en) 1990-12-30
US5364782A (en) 1994-11-15
EP0410498A3 (en) 1991-11-06
KR920701449A (ko) 1992-08-11
WO1991000353A3 (en) 1991-02-21
DK0410498T3 (da) 1999-03-22
PT94560B (pt) 1998-01-30
CA2030554C (en) 2001-08-28
EP0410498A2 (en) 1991-01-30
ATE166922T1 (de) 1998-06-15
JP3086249B2 (ja) 2000-09-11
ES2117625T3 (es) 1998-08-16
FI103285B1 (fi) 1999-05-31
AU5953890A (en) 1991-01-17
FI103285B (fi) 1999-05-31
CN1050220A (zh) 1991-03-27
FI910907A0 (fi) 1991-02-25
BG61081B1 (bg) 1996-10-31
JPH04500756A (ja) 1992-02-13
DE69032360T2 (de) 1998-12-03
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