ATE203056T1 - Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität - Google Patents

Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität

Info

Publication number
ATE203056T1
ATE203056T1 AT96944875T AT96944875T ATE203056T1 AT E203056 T1 ATE203056 T1 AT E203056T1 AT 96944875 T AT96944875 T AT 96944875T AT 96944875 T AT96944875 T AT 96944875T AT E203056 T1 ATE203056 T1 AT E203056T1
Authority
AT
Austria
Prior art keywords
dna polymerase
exonuclease activity
thermostable dna
enzymes derived
thermoanaerobacter thermohydrosulfuricus
Prior art date
Application number
AT96944875T
Other languages
English (en)
Inventor
Joseph A Mamone
Maria Davis
Dan Sha
Original Assignee
Amersham Life Science Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Life Science Inc filed Critical Amersham Life Science Inc
Application granted granted Critical
Publication of ATE203056T1 publication Critical patent/ATE203056T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
AT96944875T 1995-12-15 1996-12-13 Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität ATE203056T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US868895P 1995-12-15 1995-12-15
PCT/US1996/020225 WO1997021821A1 (en) 1995-12-15 1996-12-13 Thermostable dna polymerase from thermoanaerobacter thermohydrosulfuricus and mutant enzymes thereof with exonuclease activity removed

Publications (1)

Publication Number Publication Date
ATE203056T1 true ATE203056T1 (de) 2001-07-15

Family

ID=21733102

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96944875T ATE203056T1 (de) 1995-12-15 1996-12-13 Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität

Country Status (8)

Country Link
US (1) US5744312A (de)
EP (1) EP0866868B1 (de)
JP (1) JP4073038B2 (de)
AT (1) ATE203056T1 (de)
CA (1) CA2240334C (de)
DE (1) DE69613856T2 (de)
ES (1) ES2162132T3 (de)
WO (1) WO1997021821A1 (de)

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US6406891B1 (en) 1998-09-28 2002-06-18 Board Of Regents, The University Of Texas System Dual RT procedure for cDNA synthesis
US6238868B1 (en) * 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
US6251639B1 (en) 1999-09-13 2001-06-26 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences, using a RNA-DNA composite primer
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
JP2004513617A (ja) * 2000-06-26 2004-05-13 ニューゲン　テクノロジーズ，　インコーポレイテッド 転写に基づく核酸増幅のための方法および組成物
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AU2002228974A1 (en) 2000-12-13 2002-06-24 Nugen Technologies, Inc Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US6794141B2 (en) 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification
CA2439074A1 (en) * 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
US6946251B2 (en) 2001-03-09 2005-09-20 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences using RNA-DNA composite primers
EP1368497A4 (de) 2001-03-12 2007-08-15 California Inst Of Techn Verfahren und vorrichtung zur analyse von polynukleotidsequenzen durch asynchrone basenverlängerung
KR20010079080A (ko) * 2001-06-12 2001-08-22 김유삼 신규한 dna 중합효소의 dna 서열 및 그로부터 유래된 단백질
CA2460546A1 (en) * 2001-09-14 2003-03-27 Invitrogen Corporation Dna polymerases and mutants thereof
US20030157533A1 (en) * 2001-12-20 2003-08-21 Maria Davis Nucleic acid labeling by Thermoanaerobacter thermohydrosulfuricus DNA polymerase I variants
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US20060246434A1 (en) * 2002-03-15 2006-11-02 Erlander Mark G Nucleic acid amplification
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
WO2004092418A2 (en) * 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP1716254B1 (de) 2004-02-19 2010-04-07 Helicos Biosciences Corporation Verfahren zur analyse von polynukleotidsequenzen
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
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US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
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ATE143057T1 (de) * 1994-10-17 1996-10-15 Harvard College Dns polymerase mit veränderter nukleotid- bindungstelle

Also Published As

Publication number Publication date
WO1997021821A1 (en) 1997-06-19
DE69613856T2 (de) 2002-04-04
US5744312A (en) 1998-04-28
EP0866868A1 (de) 1998-09-30
JP4073038B2 (ja) 2008-04-09
EP0866868B1 (de) 2001-07-11
JP2000501616A (ja) 2000-02-15
CA2240334C (en) 2008-07-22
DE69613856D1 (de) 2001-08-16
CA2240334A1 (en) 1997-06-19
ES2162132T3 (es) 2001-12-16

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