ATE143057T1 - Dns polymerase mit veränderter nukleotid- bindungstelle - Google Patents
Dns polymerase mit veränderter nukleotid- bindungstelleInfo
- Publication number
- ATE143057T1 ATE143057T1 AT94203433T AT94203433T ATE143057T1 AT E143057 T1 ATE143057 T1 AT E143057T1 AT 94203433 T AT94203433 T AT 94203433T AT 94203433 T AT94203433 T AT 94203433T AT E143057 T1 ATE143057 T1 AT E143057T1
- Authority
- AT
- Austria
- Prior art keywords
- dna polymerase
- binding site
- modified nucleotide
- nucleotide binding
- modified
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US32443794A | 1994-10-17 | 1994-10-17 | |
US08/337,615 US5614365A (en) | 1994-10-17 | 1994-11-10 | DNA polymerase having modified nucleotide binding site for DNA sequencing |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE143057T1 true ATE143057T1 (de) | 1996-10-15 |
Family
ID=26984456
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT94203433T ATE143057T1 (de) | 1994-10-17 | 1994-11-24 | Dns polymerase mit veränderter nukleotid- bindungstelle |
Country Status (12)
Country | Link |
---|---|
EP (1) | EP0655506B1 (de) |
CN (1) | CN100335621C (de) |
AT (1) | ATE143057T1 (de) |
AU (1) | AU4193396A (de) |
CA (1) | CA2201885C (de) |
DE (2) | DE69400567T2 (de) |
DK (1) | DK0655506T3 (de) |
ES (1) | ES2072238T3 (de) |
FI (1) | FI120354B (de) |
GR (1) | GR950300040T1 (de) |
HU (1) | HU220839B1 (de) |
WO (1) | WO1996012042A2 (de) |
Families Citing this family (55)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5912155A (en) * | 1994-09-30 | 1999-06-15 | Life Technologies, Inc. | Cloned DNA polymerases from Thermotoga neapolitana |
US6015668A (en) * | 1994-09-30 | 2000-01-18 | Life Technologies, Inc. | Cloned DNA polymerases from thermotoga and mutants thereof |
CA2222744C (en) * | 1995-05-31 | 2008-03-25 | Amersham Life Science, Inc. | Thermostable dna polymerases |
US6001645A (en) * | 1995-06-07 | 1999-12-14 | Promega Corporation | Thermophilic DNA polymerases from thermotoga neapolitana |
US6077664A (en) * | 1995-06-07 | 2000-06-20 | Promega Corporation | Thermophilic DNA polymerases from Thermotoga neapolitana |
US5773258A (en) * | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
AU7236296A (en) * | 1995-09-08 | 1997-03-27 | Life Technologies, Inc. | Cloned dna polymerases from thermotoga and mutants thereof |
GB9524974D0 (en) * | 1995-12-06 | 1996-02-07 | Amersham Int Plc | An improved method for dye-terminator (cycle) sequencing |
JP4073038B2 (ja) * | 1995-12-15 | 2008-04-09 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | テルモアナエロバクター・テルモヒドロスルフリカスからの熱安定性dnaポリメラーゼおよびエキソヌクレアーゼ活性が除去されているその変異体酵素 |
US5789168A (en) * | 1996-05-01 | 1998-08-04 | Visible Genetics Inc. | Method for amplification and sequencing of nucleic acid polymers |
US5888736A (en) * | 1995-12-22 | 1999-03-30 | Visible Genetics, Inc. | Method, compositions and kit for detection and identification of microorganisms |
US5830657A (en) * | 1996-05-01 | 1998-11-03 | Visible Genetics Inc. | Method for single-tube sequencing of nucleic acid polymers |
DE19612684A1 (de) * | 1996-03-29 | 1997-10-02 | Gsf Forschungszentrum Umwelt | Neue Verwendung extrem thermophiler DNA-Polymerasen |
US6214555B1 (en) | 1996-05-01 | 2001-04-10 | Visible Genetics Inc. | Method compositions and kit for detection |
US6413718B1 (en) | 1996-05-01 | 2002-07-02 | Visible Genetics Inc. | Method for sequencing of nucleic acid polymers |
US6083699A (en) * | 1996-05-01 | 2000-07-04 | Visible Genetics Inc. | Method for bi-directional sequencing of nucleic acid polymers |
DE69709087T2 (de) * | 1996-05-01 | 2002-04-25 | Visible Genetics Inc | Verfahren zum nachweis und zur identifizierung von mikroorganismen |
US5827716A (en) * | 1996-07-30 | 1998-10-27 | Amersham Life Science, Inc. | Modified Pol-II type DNA polymerases |
CZ293215B6 (cs) * | 1996-08-06 | 2004-03-17 | F. Hoffmann-La Roche Ag | Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje |
US6395524B2 (en) | 1996-11-27 | 2002-05-28 | University Of Washington | Thermostable polymerases having altered fidelity and method of identifying and using same |
US6306588B1 (en) | 1997-02-07 | 2001-10-23 | Invitrogen Corporation | Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof |
DE69841023D1 (de) | 1997-03-12 | 2009-09-10 | Applied Biosystems Llc | DNA Polymerasen mit verbesserten Fähigkeiten zum Einbau von markierten Nukleotiden |
JP3172710B2 (ja) | 1997-07-07 | 2001-06-04 | 理化学研究所 | Rnaポリメラーゼ |
JP3318579B2 (ja) | 1997-07-07 | 2002-08-26 | 理化学研究所 | Dnaの塩基配列決定方法 |
US6228628B1 (en) * | 1997-07-09 | 2001-05-08 | Roche Molecular Systems | Mutant chimeric DNA polymerase |
US5882904A (en) * | 1997-08-04 | 1999-03-16 | Amersham Pharmacia Biotech Inc. | Thermococcus barossii DNA polymerase mutants |
US6346379B1 (en) * | 1997-09-11 | 2002-02-12 | F. Hoffman-La Roche Ag | Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes |
EP1071812A4 (de) * | 1998-01-23 | 2002-05-15 | Amersham Pharm Biotech Inc | Methode, reagenzlösung und kits zur dna-sequenzierung |
CA2266755A1 (en) * | 1998-04-24 | 1999-10-24 | James M. Dunn | Method for sequencing of nucleic acid polymers |
US6096875A (en) | 1998-05-29 | 2000-08-01 | The Perlein-Elmer Corporation | Nucleotide compounds including a rigid linker |
GB9907813D0 (en) * | 1999-04-06 | 1999-06-02 | Medical Biosystems Ltd | Synthesis |
JP2003507072A (ja) * | 1999-08-21 | 2003-02-25 | アマシャム・バイオサイエンス・コーポレイション | 耐塩性を改善した、E681にアミノ酸置換を有するTaqDNAポリメラーゼおよびその同族体 |
EP1226255B1 (de) | 1999-10-29 | 2006-03-29 | Stratagene California | Zusammensetzungen und methoden zur verwendung von dna polymerasen |
US20040009486A1 (en) | 1999-10-29 | 2004-01-15 | Sorge Joseph A. | Compositions and methods utilizing DNA polymerases |
AU1491401A (en) | 1999-11-23 | 2001-06-04 | Amersham Pharmacia Biotech Inc. | Improving dideoxynucleotide-triphosphate utilization by the hyper-thermophilic dna polymerase from the archaeon pyrococcus furiosus |
US6329178B1 (en) | 2000-01-14 | 2001-12-11 | University Of Washington | DNA polymerase mutant having one or more mutations in the active site |
US7179590B2 (en) | 2000-04-18 | 2007-02-20 | Roche Molecular Systems, Inc | High temperature reverse transcription using mutant DNA polymerases |
AU2007201277B2 (en) * | 2000-05-24 | 2011-10-13 | Third Wave Technologies, Inc. | Detection of RNA |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
US20050042629A1 (en) | 2002-09-13 | 2005-02-24 | Applera Corporation | Thermus scotoductus nucleic acid polymerases |
US7222059B2 (en) | 2001-11-15 | 2007-05-22 | Siemens Medical Solutions Diagnostics | Electrophoretic trace simulator |
US7148340B2 (en) | 2001-11-30 | 2006-12-12 | Applera Corporation | Thermus thermophilus nucleic acid polymerases |
WO2003048310A2 (en) * | 2001-11-30 | 2003-06-12 | Applera Corporation | Thermus oshimai nucleic acid polymerases |
WO2003048308A2 (en) | 2001-11-30 | 2003-06-12 | Applera Corporation | Thermus brockianus nucleic acid polymerases |
JP2005528114A (ja) * | 2002-05-31 | 2005-09-22 | ユニバーシティー・オブ・ワシントン | 変異性dnaポリメラーゼi突然変異体、および連続培養において変異性dnaポリメラーゼi突然変異体を用いた、標的とされたランダム突然変異誘発のための方法 |
US7309570B2 (en) | 2002-10-21 | 2007-12-18 | University Of Texas | Methods for using double-mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates |
EP1697534B1 (de) | 2003-12-01 | 2010-06-02 | Life Technologies Corporation | Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon |
CN101341247A (zh) | 2005-12-23 | 2009-01-07 | 西门子医疗保健诊断公司 | 对hcv进行基因分型的方法和试剂 |
WO2007130967A2 (en) | 2006-05-01 | 2007-11-15 | Siemens Healthcare Diagnostics Inc. | Novel oligonucleotide primers and methods for dna replication |
US10131898B2 (en) * | 2014-07-22 | 2018-11-20 | Bio-Rad Laboratories, Inc. | Buffers for use with polymerases |
CN106318924B (zh) * | 2016-10-26 | 2018-10-16 | 江南大学 | 一种催化dna合成延伸能力提高的dna聚合酶 |
CN109652499B (zh) * | 2017-10-12 | 2022-06-03 | 深圳华大智造科技股份有限公司 | 快速检测dna聚合酶3′-5′外切活性或错配的方法和试剂盒 |
CN110093410A (zh) * | 2019-05-21 | 2019-08-06 | 通用生物系统(安徽)有限公司 | 一种dna测序反应试剂及其制备方法 |
CN116218814A (zh) * | 2019-11-13 | 2023-06-06 | 深圳市真迈生物科技有限公司 | 改造的Klenow片段及应用 |
CN111826366B (zh) * | 2020-06-05 | 2022-08-02 | 广州英赞生物科技有限公司 | 一种直扩型热启动dna聚合酶及其制备方法与应用 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1338457C (en) * | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Purified thermostable enzyme |
US4942130A (en) * | 1987-01-14 | 1990-07-17 | President & Fellows Of Harvard College | T7 DNA polymerase |
US4962020A (en) * | 1988-07-12 | 1990-10-09 | President And Fellows Of Harvard College | DNA sequencing |
DE69002826T2 (de) * | 1989-02-06 | 1994-03-31 | Eastman Kodak Co | Reaktionskonzentrat zur dna-sequenzierung mit thermostabiler dna-polymerase. |
US5001050A (en) * | 1989-03-24 | 1991-03-19 | Consejo Superior Investigaciones Cientificas | PHφ29 DNA polymerase |
US5108892A (en) * | 1989-08-03 | 1992-04-28 | Promega Corporation | Method of using a taq dna polymerase without 5'-3'-exonuclease activity |
EP0595982A4 (en) * | 1991-07-24 | 1995-10-11 | Univ Partnerships Pty Ltd | Single step amplification and sequencing of nucleic acids |
-
1994
- 1994-11-24 DE DE69400567T patent/DE69400567T2/de not_active Expired - Lifetime
- 1994-11-24 AT AT94203433T patent/ATE143057T1/de active
- 1994-11-24 DK DK94203433.1T patent/DK0655506T3/da active
- 1994-11-24 ES ES94203433T patent/ES2072238T3/es not_active Expired - Lifetime
- 1994-11-24 DE DE0655506T patent/DE655506T1/de active Pending
- 1994-11-24 EP EP94203433A patent/EP0655506B1/de not_active Expired - Lifetime
-
1995
- 1995-07-31 GR GR950300040T patent/GR950300040T1/el unknown
- 1995-10-16 AU AU41933/96A patent/AU4193396A/en not_active Abandoned
- 1995-10-16 CN CNB951968297A patent/CN100335621C/zh not_active Expired - Lifetime
- 1995-10-16 CA CA002201885A patent/CA2201885C/en not_active Expired - Lifetime
- 1995-10-16 WO PCT/US1995/012928 patent/WO1996012042A2/en active IP Right Grant
- 1995-10-16 HU HU9702016A patent/HU220839B1/hu unknown
-
1997
- 1997-04-16 FI FI971611A patent/FI120354B/fi not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
HU220839B1 (en) | 2002-06-29 |
FI971611A (fi) | 1997-06-13 |
EP0655506A1 (de) | 1995-05-31 |
CA2201885A1 (en) | 1996-04-25 |
WO1996012042A2 (en) | 1996-04-25 |
EP0655506B1 (de) | 1996-09-18 |
HUT77037A (hu) | 1998-03-02 |
DE69400567D1 (de) | 1996-10-24 |
FI120354B (fi) | 2009-09-30 |
ES2072238T1 (es) | 1995-07-16 |
CA2201885C (en) | 2009-12-29 |
FI971611A0 (fi) | 1997-04-16 |
ES2072238T3 (es) | 1997-01-16 |
CN1170439A (zh) | 1998-01-14 |
DE69400567T2 (de) | 1997-02-06 |
GR950300040T1 (en) | 1995-07-31 |
DE655506T1 (de) | 1995-09-28 |
CN100335621C (zh) | 2007-09-05 |
DK0655506T3 (da) | 1997-03-10 |
WO1996012042A3 (en) | 1996-08-01 |
AU4193396A (en) | 1996-05-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE143057T1 (de) | Dns polymerase mit veränderter nukleotid- bindungstelle | |
ATE440962T1 (de) | Dns polymerasen | |
DE69729080D1 (de) | Lebensmittelechten tintenstrahltinten | |
DE69728162D1 (de) | Härtbare tintenzusammensetzung | |
DE69828517D1 (de) | Methode zur dns-isolierung. | |
DE69715833T2 (de) | Tintenstrahldrucker mit einzelnem durchgang | |
DE69701873D1 (de) | Tintenstrahltinten | |
DE69730433D1 (de) | Selbstregulierende zusammensetzung zur wasserreinigung | |
DE69835676D1 (de) | Dna polymerase-verwandte faktoren | |
DE69725221D1 (de) | Tintenstrahldrucksystem mit Temperaturkontrolle | |
DE69606158D1 (de) | Geformte Aktivkohle auf der Basis von Lignocellulose | |
PT797682E (pt) | Purificacao de uma formacao de tripla helice com um oligonucleotido imobilizado | |
DE69709856D1 (de) | Aufzeichnungsmedium, methode zur aufzeichnung auf das medium, bildenthaltendes medium | |
DE3576524D1 (de) | Dna-sequenzen. | |
MX9202037A (es) | Purificacion por filtracion de adn. | |
BR9815906B1 (pt) | método para reduzir as impurezas de uma argila de caulim. | |
DE3682516D1 (de) | Zusammensetzungen zur strassenmarkierung. | |
ID19571A (id) | Proses pemurnian 1,4-butanadiol mentah | |
FI944850A (fi) | Itsestään geeliytyviä, parantuneen varastointikestävyyden omaavia sideainehartseja offsetpainoväreihin | |
ATA206394A (de) | Wasserverdünnbare strassenmarkierfarben | |
DE59703541D1 (de) | Tintendruckkopf | |
DE69703919D1 (de) | Geformte Aktivkohle auf der Basis von Lignocellulose | |
DE3586266D1 (de) | Genomische dns menschlichen renins. | |
DE3781747D1 (de) | Fuer das rinderpestvirus-antigen kodierende dns, ihre herstellung und verwendung. | |
DE3671015D1 (de) | Organische peroxyde, tauglich zur polymerisationsregulierung. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
UEP | Publication of translation of european patent specification |