DE69629077D1 - Thermostabile DNA Polymerase - Google Patents

Thermostabile DNA Polymerase

Info

Publication number
DE69629077D1
DE69629077D1 DE69629077T DE69629077T DE69629077D1 DE 69629077 D1 DE69629077 D1 DE 69629077D1 DE 69629077 T DE69629077 T DE 69629077T DE 69629077 T DE69629077 T DE 69629077T DE 69629077 D1 DE69629077 D1 DE 69629077D1
Authority
DE
Germany
Prior art keywords
dna polymerase
polymerase
thermostable dna
thermus
homology
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69629077T
Other languages
English (en)
Other versions
DE69629077T2 (de
Inventor
Maria Davis
Carl Fuller
Bruce Moffett
John James Akron Cunniff
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
Amersham Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Corp filed Critical Amersham Biosciences Corp
Application granted granted Critical
Publication of DE69629077D1 publication Critical patent/DE69629077D1/de
Publication of DE69629077T2 publication Critical patent/DE69629077T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pulleys (AREA)
DE69629077T 1995-05-31 1996-05-30 Thermostabile DNA Polymerase Expired - Lifetime DE69629077T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US455686 1989-12-22
US45568695A 1995-05-31 1995-05-31

Publications (2)

Publication Number Publication Date
DE69629077D1 true DE69629077D1 (de) 2003-08-21
DE69629077T2 DE69629077T2 (de) 2004-04-22

Family

ID=23809855

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69629077T Expired - Lifetime DE69629077T2 (de) 1995-05-31 1996-05-30 Thermostabile DNA Polymerase

Country Status (8)

Country Link
US (1) US5885813A (de)
EP (1) EP0745676B1 (de)
JP (1) JP3966555B2 (de)
AT (1) ATE245193T1 (de)
CA (1) CA2222744C (de)
DE (1) DE69629077T2 (de)
GB (1) GB2301590B (de)
WO (1) WO1996038568A1 (de)

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
JP2000502882A (ja) * 1995-09-08 2000-03-14 ライフ・テクノロジーズ・インコーポレイテッド サーモトガ由来のクローン化ｄｎａポリメラーゼ類およびそれらの変異体
US6165765A (en) * 1995-10-18 2000-12-26 Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
US6818431B1 (en) 1995-10-18 2004-11-16 Shanghai Mendel Dna Center Co. Ltd. DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
EP1007730A4 (de) 1996-08-30 2004-04-28 Invitrogen Corp Verfahren zur identifizierung und isolierung von spezifischen nukleotidsequenzen in cdna und genomischer dna
US6306588B1 (en) 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
JP3435416B2 (ja) * 1997-03-12 2003-08-11 ピーイー コーポレイション（エヌワイ） 改善された標識ヌクレオチド取込み特性を有するｄｎａポリメラーゼ
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6130045A (en) * 1998-06-11 2000-10-10 Clontech Laboratories, Inc. Thermostable polymerase
CA2330735C (en) * 1998-06-17 2010-05-11 Amersham Pharmacia Biotech Inc. Fy7 polymerase
AU5008900A (en) * 1999-05-12 2000-11-21 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
GB9918150D0 (en) * 1999-08-03 1999-10-06 Univ Sheffield Nuclease variant
EP1210440B1 (de) * 1999-08-21 2005-07-20 Amersham Biosciences Corp. Taq dna-polymerase mit einer aminosäuresubstitution in position e681 und entsprechende homologe mit einer verbesserten salztoleranz, nützlich für die sequenzierung schwieriger templatesequenzen mittels geladenen oder ungeladenen terminatoren
JP4949585B2 (ja) * 1999-10-29 2012-06-13 アジレント・テクノロジーズ・インク Ｄｎａポリメラーゼを使用した組成物および方法
US20040009486A1 (en) 1999-10-29 2004-01-15 Sorge Joseph A. Compositions and methods utilizing DNA polymerases
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
ATE356222T1 (de) 2000-10-06 2007-03-15 Univ Columbia Massives parallelverfahren zur dekodierung von dna und rna
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
US20070020622A1 (en) * 2001-09-14 2007-01-25 Invitrogen Corporation DNA Polymerases and mutants thereof
WO2003048308A2 (en) 2001-11-30 2003-06-12 Applera Corporation Thermus brockianus nucleic acid polymerases
JP2005511043A (ja) 2001-11-30 2005-04-28 アプレラ コーポレイション Ｔｈｅｒｍｕｓｔｈｅｒｍｏｐｈｉｌｕｓ核酸ポリメラーゼ
WO2003068991A1 (en) * 2002-02-12 2003-08-21 Applera Corporation Polymerase compositions
JP4722035B2 (ja) 2003-03-25 2011-07-13 アジレント・テクノロジーズ・インク Ｄｎａポリメラーゼ融合物およびその使用
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
AU2004203649B2 (en) 2003-08-12 2006-01-12 F. Hoffmann-La Roche Ag Thermostable Taq polymerase fragment
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP1697534B1 (de) 2003-12-01 2010-06-02 Life Technologies Corporation Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
WO2005054441A2 (en) * 2003-12-01 2005-06-16 California Institute Of Technology Device for immobilizing chemical and biomedical species and methods of using same
US7981604B2 (en) 2004-02-19 2011-07-19 California Institute Of Technology Methods and kits for analyzing polynucleotide sequences
WO2006028496A2 (en) * 2004-02-20 2006-03-16 Applera Corporation Lesion repair polymerase compositions
US7417133B2 (en) * 2004-02-27 2008-08-26 Institut Pasteur Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US7635562B2 (en) * 2004-05-25 2009-12-22 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US20070117104A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070117103A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US20060118754A1 (en) * 2004-12-08 2006-06-08 Lapen Daniel C Stabilizing a polyelectrolyte multilayer
AU2005314431B2 (en) * 2004-12-11 2011-02-17 Cytogenix, Inc. Cell free biosynthesis of high-quality nucleic acid and uses thereof
US7220549B2 (en) * 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
AU2006204006A1 (en) 2005-01-06 2006-07-13 Applera Corporation Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
US20070117102A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
WO2011027966A2 (en) 2009-09-03 2011-03-10 Seegene, Inc. Td probe and its uses
EP2582804B1 (de) 2010-06-18 2015-02-25 Roche Diagnostics GmbH Dna polymerasen mit erhöhter unterscheidung von 3' fehlpaarungen
US20240102067A1 (en) * 2022-09-26 2024-03-28 Illumina, Inc. Resynthesis Kits and Methods

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5079352A (en) * 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US4795699A (en) * 1987-01-14 1989-01-03 President And Fellows Of Harvard College T7 DNA polymerase
US5075216A (en) * 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
WO1990012111A1 (en) * 1989-04-12 1990-10-18 President And Fellows Of Harvard College Improved primer extension reactions
DE69030386T2 (de) * 1989-12-22 1997-10-09 F. Hoffmann-La Roche Ag, Basel Bei hoher temperatur aktive reverse transkriptasen
JP2709311B2 (ja) * 1990-09-28 1998-02-04 エフ．ホフマン−ラ ロシュ アクチェンゲゼルシャフト 熱安定性ｄｎａポリメラーゼの５→３′のエキソヌクレアーゼ突然変異
AU8906091A (en) * 1990-10-05 1992-04-28 Wayne M. Barnes Thermostable dna polymerase
FI923911A (fi) * 1992-09-01 1994-03-02 Vsevolod Kiselev DNA-molekylers in vitro-syntes
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5474920A (en) * 1993-11-23 1995-12-12 State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University Modified thermo-resistant DNA polymerases
EP0655506B1 (de) * 1994-10-17 1996-09-18 President And Fellows Of Harvard College DNS Polymerase mit veränderter Nukleotid-Bindungstelle

Also Published As

Publication number Publication date
DE69629077T2 (de) 2004-04-22
CA2222744C (en) 2008-03-25
JP3966555B2 (ja) 2007-08-29
GB2301590B (en) 1997-07-09
US5885813A (en) 1999-03-23
WO1996038568A1 (en) 1996-12-05
ATE245193T1 (de) 2003-08-15
GB9611218D0 (en) 1996-07-31
EP0745676A1 (de) 1996-12-04
EP0745676B1 (de) 2003-07-16
JPH11507211A (ja) 1999-06-29
GB2301590A (en) 1996-12-11
CA2222744A1 (en) 1996-12-05

Similar Documents

Publication Publication Date Title
DE69629077D1 (de) Thermostabile DNA Polymerase
ATE203056T1 (de) Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität
DE69534728D1 (de) Amplifizierung von langen Nukleinsäuresequenzen mit PCR
GR3024616T3 (en) Purified thermostable enzyme and process for amplifying, detecting, and/or cloning nucleic acid sequences using said enzyme
DE69132477D1 (en) Gereinigte thermostabile nukleinsäurepolymerase aus thermosipho africanus
ATE207543T1 (de) Isotherme amplifikation von nukleinsäuren durch strangverdrängung
DE69430818D1 (de) Pns-dns-pns-chimäre makromoleküle
DE69628959D1 (de) Stabilisierte Zusammensetzungen von Reverser Transkriptase und RNA Polymerase für Nukleinsäureamplifikation
ATE274587T1 (de) 5'-nukleasen aus thermostabilen dna-polymerasen
DE69725076D1 (de) Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
DK72593D0 (da) Rekombinant protein
ES2134198T3 (es) Mutaciones en la 5' a 3' exonucleasa de las adn polimerasas.
ES2136730T3 (es) Adn polimerasas que presentan una extension de cebado de termoestabilidad, de longitud y de eficacia mejoradas.
DK0624641T3 (da) Termostabil nukleinsyrepolymerase
EP1064296A4 (de) Temperaturstabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus
DE69737024D1 (en) Thermostabiles nukleinsäure-polymerase von thermococcus gorgonarius
WO1996014405A3 (en) Biologically active fragments of thermus flavus dna polymerase
Rumyantseva et al. Use of esterase isoenzymes for identification of lucerne nodule bacteria.
KR940021734A (ko) 코리네박테리움 글루타미컴(Corynebacterium glutamicum)의 만노스 효소II를 코드하는 유전자와 그의 발현

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: GE HEALTHCARE BIO-SCIENCES CORP., PISCATAWAY, N.J.