WO1996014405A3 - Biologically active fragments of thermus flavus dna polymerase - Google Patents

Biologically active fragments of thermus flavus dna polymerase Download PDF

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Publication number
WO1996014405A3
WO1996014405A3 PCT/US1995/015327 US9515327W WO9614405A3 WO 1996014405 A3 WO1996014405 A3 WO 1996014405A3 US 9515327 W US9515327 W US 9515327W WO 9614405 A3 WO9614405 A3 WO 9614405A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna polymerase
biologically active
dna
flavus
active fragments
Prior art date
Application number
PCT/US1995/015327
Other languages
French (fr)
Other versions
WO1996014405A2 (en
Inventor
Reinhold D Mueller
Piotr M Skowron
Neela Swaminathan
Richard F Piehl
Original Assignee
Molecular Biology Resources
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Molecular Biology Resources filed Critical Molecular Biology Resources
Priority to AU42450/96A priority Critical patent/AU4245096A/en
Publication of WO1996014405A2 publication Critical patent/WO1996014405A2/en
Publication of WO1996014405A3 publication Critical patent/WO1996014405A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention is directed to a DNA encoding a biologically active fragment of a thermostable, full length DNA polymerase I enzyme of Thermus flavus. More particularly, the invention is directed to a DNA encoding an approximately 63,000 dalton DNA polymerase that lacks 274 amino acids from the N-terminus of the approximately 94,000 dalton T. flavus DNA polymerase I, and to the protein encoded thereby which has been designated the T. flavus DNA polymerase I exo fragment. The enzyme fragments are useful in DNA sequencing, Thermal Cycle Labeling, Polymerase Chain Reaction, and other molecular biological applications.
PCT/US1995/015327 1994-11-04 1995-11-03 Biologically active fragments of thermus flavus dna polymerase WO1996014405A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU42450/96A AU4245096A (en) 1994-11-04 1995-11-03 Biologically active fragments of thermus flavus dna polymerase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33464594A 1994-11-04 1994-11-04
US08/334,645 1994-11-04

Publications (2)

Publication Number Publication Date
WO1996014405A2 WO1996014405A2 (en) 1996-05-17
WO1996014405A3 true WO1996014405A3 (en) 1996-10-17

Family

ID=23308134

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/015327 WO1996014405A2 (en) 1994-11-04 1995-11-03 Biologically active fragments of thermus flavus dna polymerase

Country Status (2)

Country Link
AU (1) AU4245096A (en)
WO (1) WO1996014405A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19647055A1 (en) * 1996-11-14 1998-05-20 Boehringer Mannheim Gmbh Stabilized aqueous nucleoside triphosphate solutions
CN104293671A (en) * 2013-07-17 2015-01-21 南京朗恩生物科技有限公司 Disruption method of recombinant escherichia coli

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991009950A1 (en) * 1989-12-22 1991-07-11 F. Hoffmann-La Roche Ag Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase
WO1992003556A1 (en) * 1990-08-13 1992-03-05 F.Hoffmann-La Roche Ag PURIFIED THERMOSTABLE NUCLEIC ACID POLYMERASE ENZYME FROM $i(TERMOTOGA MARITIMA)
WO1992006200A1 (en) * 1990-09-28 1992-04-16 F. Hoffmann-La-Roche Ag 5' to 3' exonuclease mutations of thermostable dna polymerases
US5352778A (en) * 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
WO1994029482A1 (en) * 1993-06-04 1994-12-22 Third Wave Technologies, Inc. 5' nucleases derived from thermostable dna polymerase

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991009950A1 (en) * 1989-12-22 1991-07-11 F. Hoffmann-La Roche Ag Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase
US5352778A (en) * 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
WO1992003556A1 (en) * 1990-08-13 1992-03-05 F.Hoffmann-La Roche Ag PURIFIED THERMOSTABLE NUCLEIC ACID POLYMERASE ENZYME FROM $i(TERMOTOGA MARITIMA)
WO1992006200A1 (en) * 1990-09-28 1992-04-16 F. Hoffmann-La-Roche Ag 5' to 3' exonuclease mutations of thermostable dna polymerases
WO1994029482A1 (en) * 1993-06-04 1994-12-22 Third Wave Technologies, Inc. 5' nucleases derived from thermostable dna polymerase

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
AKHMETZJANOV,A.A. AND VAKHITOV,V.A.: "Molecular cloning and nucleotide sequence of the DNA polymerase gene from Thermus flavus", NUCLEIC ACIDS RESEARCH, vol. 20, no. 21, 1992, pages 5839, XP002010864 *
ASAKURA,K. ET AL.: "Cloning, nucleotide sequence, and expression in Escherichia coli of DNA polymerase gene (polA) from Thermus thermophilus HB8", J.FERMENT.BIOENG., vol. 76, no. 4, 1993, pages 265 - 269, XP002010862 *
KALEDIN,A.S. ET AL.: "Isolation and properties of DNA polymerase from the extremely thermophilic bacterium Thermus flavus", BIOCHEMISTRY (A TRANSLATION OF BIOKHIMIYA), vol. 46, no. 9, September 1981 (1981-09-01), pages 1247 - 1254, XP002010863 *

Also Published As

Publication number Publication date
AU4245096A (en) 1996-05-31
WO1996014405A2 (en) 1996-05-17

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