ATE245193T1 - Thermostabile dna polymerase - Google Patents

Thermostabile dna polymerase

Info

Publication number
ATE245193T1
ATE245193T1 AT96303880T AT96303880T ATE245193T1 AT E245193 T1 ATE245193 T1 AT E245193T1 AT 96303880 T AT96303880 T AT 96303880T AT 96303880 T AT96303880 T AT 96303880T AT E245193 T1 ATE245193 T1 AT E245193T1
Authority
AT
Austria
Prior art keywords
dna polymerase
polymerase
thermostable dna
thermus
homology
Prior art date
Application number
AT96303880T
Other languages
English (en)
Inventor
Maria Davis
Carl Fuller
Bruce Moffett
John James Cunniff
Original Assignee
Amersham Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Corp filed Critical Amersham Biosciences Corp
Application granted granted Critical
Publication of ATE245193T1 publication Critical patent/ATE245193T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
AT96303880T 1995-05-31 1996-05-30 Thermostabile dna polymerase ATE245193T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US45568695A 1995-05-31 1995-05-31

Publications (1)

Publication Number Publication Date
ATE245193T1 true ATE245193T1 (de) 2003-08-15

Family

ID=23809855

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96303880T ATE245193T1 (de) 1995-05-31 1996-05-30 Thermostabile dna polymerase

Country Status (8)

Country Link
US (1) US5885813A (de)
EP (1) EP0745676B1 (de)
JP (1) JP3966555B2 (de)
AT (1) ATE245193T1 (de)
CA (1) CA2222744C (de)
DE (1) DE69629077T2 (de)
GB (1) GB2301590B (de)
WO (1) WO1996038568A1 (de)

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
WO1997009451A1 (en) * 1995-09-08 1997-03-13 Life Technologies, Inc. Cloned dna polymerases from thermotoga and mutants thereof
US6165765A (en) * 1995-10-18 2000-12-26 Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
US6818431B1 (en) 1995-10-18 2004-11-16 Shanghai Mendel Dna Center Co. Ltd. DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
AU4174397A (en) * 1996-08-30 1998-03-19 Life Technologies, Inc. Methods for identification and isolation of specific nucleotide sequences in cdna and genomic dna
US6306588B1 (en) 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
EP2202312B1 (de) * 1997-03-12 2016-01-06 Applied Biosystems, LLC DNA-Polymerase mit verbesserten etikettierten Nucleotideinbindungseigenschaften
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6130045A (en) * 1998-06-11 2000-10-10 Clontech Laboratories, Inc. Thermostable polymerase
ATE371022T1 (de) 1998-06-17 2007-09-15 Ge Healthcare Bio Sciences Fy7 polymerase
WO2000068411A1 (en) * 1999-05-12 2000-11-16 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
GB9918150D0 (en) * 1999-08-03 1999-10-06 Univ Sheffield Nuclease variant
CA2381206A1 (en) * 1999-08-21 2001-03-01 Amersham Pharmacia Biotech Inc. Taq dna polymerases having an amino acid substitution at e681 and homologs thereof exhibiting improved salt tolerance
DE60027040T2 (de) * 1999-10-29 2006-11-23 Stratagene California, La Jolla Zusammensetzungen und methoden zur verwendung von dna polymerasen
US20040009486A1 (en) 1999-10-29 2004-01-15 Sorge Joseph A. Compositions and methods utilizing DNA polymerases
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
EP3034627B1 (de) 2000-10-06 2019-01-30 The Trustees of Columbia University in the City of New York Massives paralleles verfahren zum entschlüsseln von dna und rna
EP1436385A4 (de) * 2001-09-14 2005-12-14 Invitrogen Corp Dna-polymerasen und mutanten davon
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
EP1456220A4 (de) 2001-11-30 2005-03-09 Applera Corp Nukleinsäurepolymerasen aus thermus thermophilus
WO2003048308A2 (en) 2001-11-30 2003-06-12 Applera Corporation Thermus brockianus nucleic acid polymerases
CA2475785A1 (en) * 2002-02-12 2003-08-21 Applera Corporation Polymerase compositions
WO2004087868A2 (en) 2003-03-25 2004-10-14 Stratagene Dna polymerase fusions and uses thereof
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
AU2004203649B2 (en) 2003-08-12 2006-01-12 F. Hoffmann-La Roche Ag Thermostable Taq polymerase fragment
US7169560B2 (en) * 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP2287341B1 (de) 2003-12-01 2013-02-13 Life Technologies Corporation Rekombinationsstellen enthaltende Nukleinsäuremoleküle und Verfahren zur Verwendung davon
US20060172408A1 (en) * 2003-12-01 2006-08-03 Quake Steven R Device for immobilizing chemical and biochemical species and methods of using same
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US20050196392A1 (en) * 2004-02-20 2005-09-08 Andersen Mark R. Lesion repair polymerase compositions
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US7417133B2 (en) 2004-02-27 2008-08-26 Institut Pasteur Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US20070117104A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070117103A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
DE602005027700D1 (de) * 2004-05-25 2011-06-09 Helicos Biosciences Corp Verfahren zur nukleinsäureimmobilisierung
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US20060118754A1 (en) * 2004-12-08 2006-06-08 Lapen Daniel C Stabilizing a polyelectrolyte multilayer
BRPI0515777A (pt) * 2004-12-11 2008-08-05 Cytogenix Inc biossìntese isenta de células de ácido nucléico de alta qualidade e usos dos mesmos
US7220549B2 (en) * 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
US20060228726A1 (en) 2005-01-06 2006-10-12 Martin Patrick K Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
US20070117102A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
WO2011027966A2 (en) 2009-09-03 2011-03-10 Seegene, Inc. Td probe and its uses
JP5876479B2 (ja) 2010-06-18 2016-03-02 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 増大した3’末端ミスマッチ識別を有するdnaポリメラーゼ
US20240102067A1 (en) * 2022-09-26 2024-03-28 Illumina, Inc. Resynthesis Kits and Methods

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5079352A (en) * 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US4795699A (en) * 1987-01-14 1989-01-03 President And Fellows Of Harvard College T7 DNA polymerase
US5075216A (en) * 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
WO1990012111A1 (en) * 1989-04-12 1990-10-18 President And Fellows Of Harvard College Improved primer extension reactions
DK0506889T3 (da) * 1989-12-22 1997-09-22 Hoffmann La Roche Reverse transkriptaser ved høj temperatur
JP2709311B2 (ja) * 1990-09-28 1998-02-04 エフ.ホフマン−ラ ロシュ アクチェンゲゼルシャフト 熱安定性dnaポリメラーゼの5→3′のエキソヌクレアーゼ突然変異
WO1992006188A2 (en) * 1990-10-05 1992-04-16 Barnes Wayne M Thermostable dna polymerase
FI923911A (fi) * 1992-09-01 1994-03-02 Vsevolod Kiselev DNA-molekylers in vitro-syntes
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5474920A (en) * 1993-11-23 1995-12-12 State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University Modified thermo-resistant DNA polymerases
DE655506T1 (de) * 1994-10-17 1995-09-28 Harvard College DNS Polymerase mit Veränderten Nukleotiden Bindungstelle.

Also Published As

Publication number Publication date
CA2222744A1 (en) 1996-12-05
EP0745676A1 (de) 1996-12-04
WO1996038568A1 (en) 1996-12-05
GB2301590A (en) 1996-12-11
JP3966555B2 (ja) 2007-08-29
GB2301590B (en) 1997-07-09
CA2222744C (en) 2008-03-25
US5885813A (en) 1999-03-23
DE69629077T2 (de) 2004-04-22
GB9611218D0 (en) 1996-07-31
EP0745676B1 (de) 2003-07-16
DE69629077D1 (de) 2003-08-21
JPH11507211A (ja) 1999-06-29

Similar Documents

Publication Publication Date Title
ATE245193T1 (de) Thermostabile dna polymerase
DE69613856T2 (de) Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität
DE69524653T2 (de) Amplifizierung von langen Nukleinsäuresequenzen mit PCR
DE69132477D1 (en) Gereinigte thermostabile nukleinsäurepolymerase aus thermosipho africanus
ATE207543T1 (de) Isotherme amplifikation von nukleinsäuren durch strangverdrängung
ATE391771T1 (de) Stabile enzymzusammensetzung, die eine thermostabile nukleinsäurepolymerase enthält
ATE219095T1 (de) Pns-dns-pns-chimäre makromoleküle
ATE244759T1 (de) Stabilisierte zusammensetzungen von reverser transkriptase und rna polymerase für nukleinsäureamplifikation
DK72593D0 (da) Rekombinant protein
DE69725076D1 (de) Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
ATE181573T1 (de) Dns-polymerasen mit erhöhter temperaturstabilität und vergrössterter länge und effizient der primer-verlängerung
ES2134198T3 (es) Mutaciones en la 5' a 3' exonucleasa de las adn polimerasas.
DK0624641T3 (da) Termostabil nukleinsyrepolymerase
EP1064296A4 (de) Temperaturstabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus
DE69737024D1 (en) Thermostabiles nukleinsäure-polymerase von thermococcus gorgonarius
WO1996014405A3 (en) Biologically active fragments of thermus flavus dna polymerase
KR940021734A (ko) 코리네박테리움 글루타미컴(Corynebacterium glutamicum)의 만노스 효소II를 코드하는 유전자와 그의 발현

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties